Abstract:
:Electrochemotherapy (ECT), chemotherapy administered in combination with electric fields, has the potential to be an effective localized treatment for cutaneous malignancies. Bleomycin's cytotoxicity was enhanced by exposing tumour cells to electrical fields following intravenous injection of the chemotherapeutic agent. Two issues associated with this procedure are the existence of a narrow but optimal time-window for effective treatment and the fact that a systemic drug dose is administered for a localized therapy. In order to address these issues, a study was initiated to examine the effectiveness of administering bleomycin by intratumoural injection. A dose-response relationship for intratumoural injection was determined. In addition, the minimal effective field strength necessary for ECT was established. Results of this study indicated drastic reductions in tumour volume for ECT-treated groups. In addition, ECT-treated groups showed increased survival over control groups. The minimum effective dose for the ECT intratumour bleomycin group was 0.025 units. The minimum effective field strength was found to be 1250 V/cm. The results demonstrate that intratumoural injection of bleomycin in combination with electric pulses is effective, and this information will be used to initiate clinical trials.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Heller R,Jaroszeski M,Perrott R,Messina J,Gilbert Rdoi
10.1097/00008390-199702000-00003subject
Has Abstractpub_date
1997-02-01 00:00:00pages
10-8issue
1eissn
0960-8931issn
1473-5636journal_volume
7pub_type
杂志文章abstract::In this study we investigated the antitumour effect and mechanism of action of cationic liposome-mediated murine interferon-beta (IFNbeta) gene therapy in mouse B16F1 melanoma cells in vitro and in vivo. Murine IFNbeta gene transfer by cationic liposome resulted in substantial growth inhibition of B16F1 melanoma cells...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200308000-00003
更新日期:2003-08-01 00:00:00
abstract::The nm23 gene is a putative tumour and metastasis suppressor gene. A number of studies have found that reduction of its expression is associated with increased metastatic potential in several human malignancies. Similarly, clinical studies have shown correlation between reduced nm23 protein expression and a poor progn...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200402000-00004
更新日期:2004-02-01 00:00:00
abstract::Prognostic blood biomarkers for patients with uveal melanoma have not been identified. Tumor monosomy-3 is strongly associated with the development of metastatic disease. Tumor expression of human leukocyte antigen class I molecules and insulin-like growth factor (IGF)-1 receptor has also been associated with the deve...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835b7154
更新日期:2013-02-01 00:00:00
abstract::Prognostic groups defined by lactate dehydrogenase concentration and number of organ sites containing metastases have been reported for patients treated with dabrafenib and trametinib for advanced melanoma. We aimed to validate these prognostic groups for patients treated with vemurafenib and cobimetinib in the coBRIM...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000634
更新日期:2020-06-01 00:00:00
abstract::An increased risk of melanoma has been variously reported in patients with chronic lymphocytic leukaemia (CLL), analogous with other immunosuppressed populations. To fully assess this association, we performed a systematic review and meta-analysis of available evidence from observational cohort studies. All such longi...
journal_title:Melanoma research
pub_type: 杂志文章,meta分析,评审
doi:10.1097/CMR.0000000000000219
更新日期:2016-04-01 00:00:00
abstract::Cell free extracts from metastases of human melanoma contain a highly active ribonucleoside diphosphate reductase (RR) which uses guanosine diphosphate (GDP) as substrate and deoxythymidine triphosphate (dTTP) as effector. No activity could be detected in these extracts when cytidine diphosphate (CDP) was used as the ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199212000-00014
更新日期:1992-12-01 00:00:00
abstract::Melanogenesis appears to be a unique target to develop anti-tumour agents specific for malignant melanoma. Among the anti-melanoma compounds that we have examined, 4-S-cysteaminylphenol (4-S-CAP), a phenolic amine, was found to have the most promising anti-melanoma effects. To further improve the efficacy as anti-mela...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200404000-00006
更新日期:2004-04-01 00:00:00
abstract::The universal second messenger cAMP regulates numerous cellular processes. Although the cAMP-signaling pathway leads to induction of gene transcription, it remains unknown whether this pathway contributes toward suppression of transcription. Here, we show that blockade of cAMP signaling using MDL12330A led to an incre...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000363
更新日期:2017-10-01 00:00:00
abstract::Significant advances have been made in the treatment of melanoma by targeting key cellular pathways, but additional targets are needed as many patients do not respond or relapse with resistant disease. MicroRNA-155 (MiR-155) has previously been shown to regulate melanoma cell growth and acts as a tumor suppressor. We ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000545
更新日期:2019-04-01 00:00:00
abstract::The growth rate (GR) of melanomas is not uniform. A fast-growing subtype has been identified and seems to have a role in the stabilization of the mortality rates because of melanoma. To examine features associated with fast-growing melanomas (FGMs) and to determine the relationship between the GR and well-recognized p...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328342f312
更新日期:2011-04-01 00:00:00
abstract::Resistance to various antineoplastic agents is common in the clinical management of malignant melanoma. The biological mechanisms conferring these different drug-resistant phenotypes are still unclear. To identify potential factors mediating drug resistance to melanoma cells, the mRNA expression profiles of the parent...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000215037.23188.58
更新日期:2006-04-01 00:00:00
abstract::The aim of this study was to evaluate S100B in bone marrow (BM) plasma from malignant melanoma patients. BM aspirates and peripheral blood (PB) plasma from 56 patients and BM aspirates from 29 healthy volunteers were collected. S100B was measured using an immune radiometric assay, which is a two-site sandwich assay ba...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3282f623d9
更新日期:2008-04-01 00:00:00
abstract::Paraffin-embedded tissue from the primary tumours of 116 patients with malignant melanoma, and in 40 cases also from corresponding metastases, were examined for accumulation of p53 protein. The fraction of tumours with positive p53 immunostaining was 13% in the least invasive and 36% in the most invasive primary lesio...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199506000-00007
更新日期:1995-06-01 00:00:00
abstract::Single-agent anti-PD1 antibodies are usually very well tolerated, but serious toxicity can still occur. Despite the PD-1 pathway seems to be relevant in the pathogenesis of immune-related myositis, anti-PD1-related myositis is generally a rare side effect of the treatment and usually not serious. However, its frequenc...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000706
更新日期:2021-02-01 00:00:00
abstract::The aim was to evaluate toxicity and oncological outcome of combined stereotactic radiosurgery (SRS) and immunotherapy or targeted therapy in patients with multiple brain metastases originating from malignant melanoma. Despite the fact that both SRS and kinase inhibitors or immune checkpoint inhibitors are considered ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000542
更新日期:2019-04-01 00:00:00
abstract::In this study we compared the strength of the association of constitutional factors and sun exposure with cutaneous malignant melanoma (CMM) and basal cell carcinoma (BCC). We analysed 260 incident cases of CMM, 425 incident cases of BCC and two sets of population controls from previous case-control studies conducted ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199812000-00013
更新日期:1998-12-01 00:00:00
abstract::Genes in the S100 family are abnormally expressed in a variety of tumor cells and are associated with clinical pathology, but their prognostic value in melanoma patients has not yet been fully elucidated. In this study, we extracted and profiled S100 family mRNA expression data and corresponding clinical data from the...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000512
更新日期:2019-02-01 00:00:00
abstract::Radioiodinated N-(2-(diethylamino)ethyl)benzamides have recently been discovered as selective agents for melanotic melanoma and are used for scintigraphic imaging in nuclear medicine. Owing to the high binding capacity, benzamide derivatives conjugated with alkylating cytostatics were synthesized and tested for their ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200410000-00004
更新日期:2004-10-01 00:00:00
abstract::When cancer cells, including melanoma cells, are genetically altered to secrete cytokines, irradiated and injected into subjects, long-term antitumour immunity is induced. Optimally, existing melanomas induced to produce cytokines in vivo could stimulate this same immune response. Although in vivo electroporation enha...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200012000-00010
更新日期:2000-12-01 00:00:00
abstract::Pelvic sentinel lymph nodes (SLNs) are commonly identified during inguinal SLN biopsy for melanoma, but retrieval is not uniform among surgeons/centers. Few studies have assessed rates of micrometastases in pelvic versus superficial inguinal SLNs. Previous studies suggested that presence of pelvic SLNs was predicted b...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000498
更新日期:2019-02-01 00:00:00
abstract::Ipilimumab (anti-CTLA-4 antibody) is a new tool for the treatment of metastatic melanoma patients that has led to an improvement in survival rates worldwide. New types of toxicities have been described with ipilimumab called 'immune-related adverse events' or irAEs. Here, we report an acute and steroid resistant case ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835fb524
更新日期:2013-06-01 00:00:00
abstract::Staging of melanoma patients by means of whole body functional imaging in a single evaluation session using positron emission tomography (PET) with fluorine-18- labelled deoxy-d-glucose (FDG) as a metabolic tracer has created much interest over the last decade. After enthusiastic pilot studies, more attention has been...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200209000-00010
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Melanoma is the leading cause of death from skin cancers and its etiology is complex. Recent discoveries related to genetic risk factors are helping us to understand melanoma pathogenesis better. Nuclear factor-κB (NF-κB) has a critical role in immunity, inflammation, and tumor growth. The 94ins/del ATTG (rs...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000267
更新日期:2016-08-01 00:00:00
abstract::The proto-oncogene c-Kit encodes a membrane receptor protein with intrinsic tyrosine kinase activity. Activation of c-Kit induces cell proliferation, differentiation or migration among different cell types. The present study provides evidence that c-Kit plays an important role in the cell differentiation rather than i...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199510000-00002
更新日期:1995-10-01 00:00:00
abstract::The liver is the predominant site of metastases in the majority of patients with uveal melanoma, suggesting the evaluation of regional treatment approaches. Here we report our experience with transarterial chemoembolization (TACE) in uveal melanoma patients with pretreated liver metastases. Twenty-five patients were t...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328334c36e
更新日期:2010-06-01 00:00:00
abstract::The aim of this study was to present the epidemiological, clinicopathological, and treatment characteristics of patients diagnosed and treated in a tertiary referral center and to analyze independent factors associated with these characteristics. In this cohort study, epidemiological, clinicopathological, and treatmen...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000456
更新日期:2018-08-01 00:00:00
abstract::Immunosuppressed patients are at increased risk of skin cancer. A 67-year-old renal transplant recipient developed a nodular malignant melanoma after 30 years of immunosuppression with azathioprine and prednisolone. The patient died of metastatic disease 3 months after the diagnosis was made. The function of the renal...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32831bc44a
更新日期:2009-08-01 00:00:00
abstract::Melanomas are promising targets for immunotherapy, as they express a number of tissue-specific antigens against which immune responses can be elicited. We have previously described transgenic mice in which malignant cutaneous melanomas are produced. The 1042 melanoma cell line, derived from a primary melanoma in one o...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200412000-00019
更新日期:2004-12-01 00:00:00
abstract::Several hypotheses have explicitly implicated the role of an altered redox status of melanin in the aetiology of melanoma and macular degeneration. The balance between the intrinsic anti-oxidant and pro-oxidant properties of melanin is lost, resulting in an altered redox phenotype. We propose that such an alteration o...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/01.cmr.0000195699.35143.df
更新日期:2006-02-01 00:00:00
abstract::The transcription factor CUTL1 (CCAAT displacement protein 1) has been reported to participate in the proliferation of diverse types of cancer. In the present study, we investigated the potential involvement of CUTL1 in the proliferation of malignant melanoma. We found that CUTL1 expression was upregulated in malignan...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000064
更新日期:2014-06-01 00:00:00