Abstract:
:Dissemination of uveal melanomas is almost exclusively haematogenous, making angiogenesis of the tumour a prerequisite for the formation of metastases. Uveal melanomas must employ strategies to evade the immune system in order to escape immune surveillance. We therefore determined the expression of the following angiogenic and immunosuppressive factors in seven human uveal melanoma cell lines using reverse transcriptase-polymerase chain reaction (RT-PCR): secreted interleukin-1 receptor antagonist (sIL-1ra), interleukin (IL)-6, IL-8, IL-10, transforming growth factor (TGF)-alpha, TGFbeta, vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF), basic fibroblast growth factor (bFGF), angiopoietin-1 and angiopoietin-2. In addition, the secretion of sIL-1ra, IL-6, IL-8, IL-10, TGFbeta and VEGF was assayed by enzyme linked immunosorbent assay (ELISA). The potential of uveal melanoma cell lines to convert plasminogen to angiostatin was tested in an in vitro assay. All the factors except angiopoietin-1 were determined in one or more cell lines using RT-PCR, although these results were not necessarily confirmed by ELISA. Expression of VEGF and angiopoietin-2 was found in all seven cell lines. Production of angiostatin was observed in one cell line. All seven cell lines examined expressed angiogenic factors and most cell lines expressed immunosuppressive factors. The expression of VEGF and angiopoietin-2 in combination with a lack of angiopoietin-1 expression suggest high vascular remodelling capacity and could be of great relevance for the metastatic potential of uveal melanoma.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Ijland SA,Jager MJ,Heijdra BM,Westphal JR,Peek Rdoi
10.1097/00008390-199910000-00003subject
Has Abstractpub_date
1999-10-01 00:00:00pages
445-50issue
5eissn
0960-8931issn
1473-5636journal_volume
9pub_type
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,多中心研究
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pub_type: 杂志文章
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journal_title:Melanoma research
pub_type: 杂志文章,多中心研究
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journal_title:Melanoma research
pub_type: 杂志文章
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journal_title:Melanoma research
pub_type: 杂志文章
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