当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > HIV protease inhibitor ritonavir increases endothelial monolayer permeability.

HIV protease inhibitor ritonavir increases endothelial monolayer permeability.

Abstract：

:HIV protease inhibitors (PIs) are often associated with metabolic and cardiovascular complications although they are effective anti-HIV drugs. In this study, we determined whether HIV PI ritonavir could increase endothelial permeability, one of the important mechanisms of vascular lesion formation. Human dermal microvascular endothelial cells (HMECs) treated with ritonavir showed a significant increase of endothelial permeability in a dose- and time-dependent manner assayed with a transwell system. Ritonavir significantly reduced the mRNA levels of tight junction proteins zonula occluden-1, occludin, and claudin-1 by 40-60% as compared to controls (P<0.05) by real-time PCR analysis. Protein levels of these tight junction molecules were also substantially reduced in the ritonavir-treated cells. In addition, HMECs treated with ritonavir (7.5, 15, and 30microM) showed a substantial increase of superoxide anion production by 10%, 32%, and 65%, respectively, as compared to controls. Antioxidants (EGCG and SeMet) effectively reduced ritonavir-induced endothelial permeability. Furthermore, ritonavir activated ERK1/2 (phosphorylation), but not P38 and JNK. Specific ERK1/2 inhibitor, PD89059, significantly abolished ritonavir-induced endothelial permeability by 92%. Thus, HIV PI ritonavir increases endothelial permeability, decreases levels of tight junction proteins, and increases superoxide anion production. ERK1/2 activation is involved in the signal transduction pathway of ritonavir-induced endothelial permeability.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Chen C,Lu XH,Yan S,Chai H,Yao Q

doi

10.1016/j.bbrc.2005.07.155

subject

Has Abstract

pub_date

2005-09-30 00:00:00

pages

874-82

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)01633-5

journal_volume

335

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Nuclear localization signals in the core protein of hepatitis C virus.

    abstract：:The core protein of the hepatitis C virus is derived from the N-terminal 191 amino acids of the viral polyprotein by proteolytic cleavage. In the current study, subcellular localizations of the HCV core and its beta-galactosidase fusion proteins in transfected cells were examined by indirect immunofluorescence and cyt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2804

    authors： Chang SC,Yen JH,Kang HY,Jang MH,Chang MF

    更新日期：1994-12-15 00:00:00

  • Organization of human ACAT-2 gene and its cell-type-specific promoter activity.

    abstract：:Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4612

    authors： Song BL,Qi W,Yang XY,Chang CC,Zhu JQ,Chang TY,Li BL

    更新日期：2001-03-30 00:00:00

  • Evidence for translational repression of the SOCS-1 major open reading frame by an upstream open reading frame.

    abstract：:The suppressor of cytokine signalling 1 protein (SOCS-1) belongs to a novel family of cytokine inducible factors which function as inhibitors of the JAK/STAT pathway. While SOCS-1 previously has been described as a single-exon gene, here we present evidence for an additional 5' exon, separated by a 509 bp intron from ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2109

    authors： Schlüter G,Boinska D,Nieman-Seyde SC

    更新日期：2000-02-16 00:00:00

  • In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining.

    abstract：:Genome editing technology using programmable nucleases has rapidly evolved in recent years. The primary mechanism to achieve precise integration of a transgene is mainly based on homology-directed repair (HDR). However, an HDR-based genome-editing approach is less efficient than non-homologous end-joining (NHEJ). Rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.130

    authors： Shin JH,Jung S,Ramakrishna S,Kim HH,Lee J

    更新日期：2018-07-07 00:00:00

  • Genomic organization, promoter cloning, and chromosomal localization of the Dif-2 gene.

    abstract：:We describe the genomic organization and the functional promoter of the monocyte specific gene Dif-2, the human homologue to genes in mouse (gly96) and rat (PRG1), that is downregulated during cell differentiation. The Dif-2 gene consists of two exons and a single intron of 112 bp in length. RNase protection assay ind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8500

    authors： Pietzsch A,Büchler C,Schmitz G

    更新日期：1998-04-28 00:00:00

  • Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function.

    abstract：:We report here on the cloning and functional characterization of human LAT1, a subunit of the amino acid transport system L. The hLAT1 cDNA, obtained from a human placental cDNA library, codes for a protein of 507 amino acids. When functionally expressed in mammalian cells together with the heavy chain of the rat 4F2 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0206

    authors： Prasad PD,Wang H,Huang W,Kekuda R,Rajan DP,Leibach FH,Ganapathy V

    更新日期：1999-02-16 00:00:00

  • A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum.

    abstract：:From the inner shoots of the chive Allium tuberosum, a single-chained protein with a molecular weight of 36 kDa and an N-terminal sequence manifesting resemblance to chitinases but lacking in cysteine residues characteristic of a cysteine-rich domain present in chitinases of other Allium species, was purified. The iso...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3821

    authors： Lam YW,Wang HX,Ng TB

    更新日期：2000-12-09 00:00:00

  • Chondrocyte-specific ablation of Osterix leads to impaired endochondral ossification.

    abstract：:Osterix (Osx) is an essential transcription factor required for osteoblast differentiation during both intramembranous and endochondral ossification. Endochondral ossification, a process in which bone formation initiates from a cartilage intermediate, is crucial for skeletal development and growth. Osx is expressed in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.064

    authors： Oh JH,Park SY,de Crombrugghe B,Kim JE

    更新日期：2012-02-24 00:00:00

  • The soluble metalloendoprotease required in myoblast fusion remains intracellular.

    abstract：:The fusion of myoblasts to myotubes requires an endogenous soluble metalloendoprotease. To determine whether this protease is released by fusing myoblasts, or stays within the cell, we examined the effects of membrane-impermeant and a membrane-permeant metalloendoprotease inhibitors. Membrane-permeant 1,10-phenanthrol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80145-6

    authors： Farach HA Jr,Strittmatter WJ

    更新日期：1987-08-31 00:00:00

  • Solution structure of human steroidogenic acute regulatory protein STARD1 studied by small-angle X-ray scattering.

    abstract：:Intracellular cholesterol transfer to mitochondria, a bottleneck of adrenal and gonadal steroidogenesis, relies on the functioning of the steroidogenic acute regulatory protein (StAR, STARD1), for which many disease-associated mutations have been described. Despite significant progress in the field, the exact mechanis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.167

    authors： Sluchanko NN,Tugaeva KV,Maksimov EG

    更新日期：2017-08-05 00:00:00

  • Amphitrite ornata dehaloperoxidase: enhanced activity for the catalytically active globin using MCPBA.

    abstract：:Dehaloperoxidase (DHP) from Amphitrite ornata is the only heme-containing, hydrogen peroxide-dependent globin capable of oxidatively dehalogenating halophenols to yield the corresponding quinones. To ascertain that this enzymatic activity is intrinsic to DHP, we have cloned and expressed the enzyme in Escherichia coli...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.174

    authors： Osborne RL,Taylor LO,Han KP,Ely B,Dawson JH

    更新日期：2004-11-26 00:00:00

  • Both nitric oxide and nitrite prevent homocysteine-induced endoplasmic reticulum stress and subsequent apoptosis via cGMP-dependent pathway in neuronal cells.

    abstract：:Growing evidence indicates that endoplasmic reticulum (ER) stress and/or ER stress-mediated apoptosis may play a role in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease. The present study investigated the effects of non-cytotoxic concentrations of nitric oxide (NO) and nitrite, a metabolite ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.054

    authors： Jeong SO,Son Y,Lee JH,Choi SW,Kim SH,Cheong YK,Chung HT,Pae HO

    更新日期：2017-11-04 00:00:00

  • Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases.

    abstract：:Sulfation is an important pathway in the metabolism of thyroid hormones. Sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal human fetal circulation. We assayed and characterized COS-1 cell expressed recombinant human liver dehydroepiandrosterone sulfotransferase (DHEA ST or SULT2A1) and e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1419

    authors： Li X,Anderson RJ

    更新日期：1999-10-05 00:00:00

  • Glycyrrhetinic acid alleviates acute lung injury by PI3K/AKT suppressing macrophagic Nlrp3 inflammasome activation.

    abstract：:Glycyrrhetinic acid (GA), a triterpene saponins, has been widely proven to have multiple medicinal properties. Our study aimed to figure out the protective effect of GA on acute lung injury (ALI) and the underlying mechanism. The LPS-induced ALI model mice were intratracheally administrated with 10 mg/kg LPS. Pretreat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.08.044

    authors： Wang K,Zhang Y,Cao Y,Shi Z,Lin Y,Chen Y,Zhao H,Liu X

    更新日期：2020-11-19 00:00:00

  • Physical activity in the androgen receptor knockout mouse: evidence for reversal of androgen deficiency on cancellous bone.

    abstract：:Disruption of the androgen receptor (AR) in male mice reduces cortical bone expansion and muscle mass during puberty and results in high bone turnover-related cancellous osteopenia. We hypothesized that voluntary wheel running during growth is able to rescue the effects of AR disruption on bone. To this end, 5-week-ol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.016

    authors： Ophoff J,Callewaert F,Venken K,De Gendt K,Ohlsson C,Gayan-Ramirez G,Decramer M,Boonen S,Bouillon R,Verhoeven G,Vanderschueren D

    更新日期：2009-01-02 00:00:00

  • Cathepsin Q, a novel lysosomal cysteine protease highly expressed in placenta.

    abstract：:The complete nucleotide sequence of a novel cathepsin cDNA derived from rat placenta was determined and is termed cathepsin Q. The predicted protein of 343 amino acid is a member of the family C1A protease related to cathepsin L. Rat cathepsin Q and its mouse counterpart were found highly expressed in placenta, wherea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.2051

    authors： Sol-Church K,Frenck J,Mason RW

    更新日期：2000-01-27 00:00:00

  • Nucleotide sequence of a full-length cDNA for human fibroblast poly(ADP-ribose) polymerase.

    abstract：:The complete nucleotide sequence of human fibroblast poly(ADP-ribose) polymerase cDNA was determined. The cDNA contains an open reading frame for a 1014 amino acid polypeptide. In the DNA binding domain of poly(ADP-ribose) polymerase, there are predicted alpha-helix-turn-alpha-helix structures and two sequences each o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90921-1

    authors： Uchida K,Morita T,Sato T,Ogura T,Yamashita R,Noguchi S,Suzuki H,Nyunoya H,Miwa M,Sugimura T

    更新日期：1987-10-29 00:00:00

  • Characteristics of alpha-adrenoceptors in two human colorectal cancer cell lines.

    abstract：:The DiFi and HT-29 human colorectal cancer cell lines were characterized and compared with respect to binding properties of alpha adrenoceptors present on the cell surface. Both cell lines possessed alpha-1 and alpha-2 adrenoceptors of high affinity; however, DiFi cells were rich in alpha-1 adrenoceptors, whereas HT-2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80972-6

    authors： Agrawal DK,Wildrick DM,Boman BM

    更新日期：1992-05-29 00:00:00

  • Activation of the p21(Waf1/Cip1) promoter by the ets oncogene family transcription factor E1AF.

    abstract：:p21(Waf1/Cip1) is one of the key regulatory proteins in cell cycle, terminal differentiation, and apoptosis. Its promoter was shown to be transactivated by the wild-type p53 protein as well as in a p53-independent manner. In this report, we demonstrate that E1AF, an ets-related transcription factor, activates the huma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6909

    authors： Funaoka K,Shindoh M,Yoshida K,Hanzawa M,Hida K,Nishikata S,Totsuka Y,Fujinaga K

    更新日期：1997-07-09 00:00:00

  • The sequence of sites recognised by a member of the RNase E/G family can control the maximal rate of cleavage, while a 5'-monophosphorylated end appears to function cooperatively in mediating RNA binding.

    abstract：:Members of the RNase E/G family are multimeric, 5'-end-sensing, single-strand-specific endoribonucleases that are found in chloroplasts as well as bacteria, and have central roles in RNA processing and degradation. A well-studied member of this family is Escherichia coli RNase G. Recently, we have shown that the inter...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.156

    authors： Jourdan SS,Kime L,McDowall KJ

    更新日期：2010-01-01 00:00:00

  • Detection and analysis of the human D4 dopamine receptor gene (DRD4) sequence variant in the Japanese and Mongolian population.

    abstract：:A PCR-based Sma I restriction fragment length polymorphism (RFLP) in the human D4 dopamine receptor (DRD4) gene was investigated in 200 subjects each of Japanese and Mongolian populations. When compared to allele frequencies for Caucasians previously reported by Petronis et al., Japanese and Mongolian populations show...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0479

    authors： Nakatome M,Honda K,Tun Z,Wakasugi C

    更新日期：1996-03-27 00:00:00

  • Specific desensitization of actin polymerization of bovine platelets.

    abstract：:Polymerization of actin induced by activation of platelets was investigated using deoxyribonuclease I inhibition assay. When platelets were activated with ADP or 5-hydroxytryptamine, actin was polymerized quickly followed by rapid depolymerization to the initial level. Reactivation with the same agonist, however, did ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80184-9

    authors： Imada T,Kikuchi T,Shimada H,Inada Y,Saito Y

    更新日期：1984-05-16 00:00:00

  • Differential control of the metal-mediated activation of the human heme oxygenase-1 and metallothionein IIA genes.

    abstract：:Heme oxygenase-1, an essential enzyme in heme catabolism, and metallothionein IIA, a small metal-binding protein with clusters of cysteins, are remarkably induced in HeLa cells following the treatment with cadmium or zinc. Both proteins are considered to be involved in the defense system against metal toxicity. Here w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1167

    authors： Takeda K,Fujita H,Shibahara S

    更新日期：1995-02-06 00:00:00

  • UDP-glucuronosyltransferase 1A7 polymorphisms are associated with liver cirrhosis.

    abstract：:Variations in the UDP-glucuronosyltransferase (UGT) 1A7 gene have been found to be related to the development of hepatocellular carcinoma (HCC). Since the pathogenesis of liver cirrhosis is not dissimilar to that of HCC, we hypothesized that UGT1A7 genetic polymorphisms may be associated with liver cirrhosis. PCR-rest...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.125

    authors： Tang KS,Lee CM,Teng HC,Huang MJ,Huang CS

    更新日期：2008-02-15 00:00:00

  • Visualization of local phosphatidylcholine synthesis within hippocampal neurons using a compartmentalized culture system and imaging mass spectrometry.

    abstract：:Neurons extend neurites with an increased synthesis of phosphatidylcholine (PC) that is not only a membrane component but also a functional regulator with specific fatty acid composition. To analyze the local synthesis of the PC molecular species within neurons, we combined a compartmentalized culture system with matr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.108

    authors： Sugiyama E,Yao I,Setou M

    更新日期：2018-01-01 00:00:00

  • 3-D Model of the bee venom acid phosphatase: insights into allergenicity.

    abstract：:The acid phosphatase Api m 3 is the major allergen of the honeybee venom. Except for the amino acid sequence, no other structural information for the enzyme is available. We applied homology modeling to assign the three-dimensional structure of Api m 3. The structure of the homodimeric human prostatic acid phosphatase...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.101

    authors： Georgieva D,Greunke K,Genov N,Betzel C

    更新日期：2009-01-23 00:00:00

  • Specific uptake of asialofetuin-tacked liposomes encapsulating interferon-gamma by human hepatoma cells and its inhibitory effect on hepatitis B virus replication.

    abstract：:Asialofetuin-tacked liposomes (AF-liposomes) encapsulating interferon (IFN)-gamma were bound and internalized into a human hepatoma cell line, HepG2 cells, selectively through asialoglycoprotein receptor, but not non-tacked liposomes (N-liposomes). AF-liposomal IFN-gamma was more effective for inhibition of viral DNA ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91494-w

    authors： Ishihara H,Hayashi Y,Hara T,Aramaki Y,Tsuchiya S,Koike K

    更新日期：1991-01-31 00:00:00

  • Expression and characterization of the olfactomedin domain of human myocilin.

    abstract：:The olfactomedin-domain has been first identified in olfactomedin, an extracellular matrix protein of the olfactory neuroepithelium. Members of this extracellular domain-family have since been shown to be present in several metazoan proteins, such as latrophilins, myocilins, and noelins, but their biological function ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00198-0

    authors： Nagy I,Trexler M,Patthy L

    更新日期：2003-03-14 00:00:00

  • miR-130b targets NKD2 and regulates the Wnt signaling to promote proliferation and inhibit apoptosis in osteosarcoma cells.

    abstract：:miR-130b was significantly up-regulated in osteosarcoma (OS) cells. Naked cuticle homolog 2 (NKD2) inhibited tumor growth and metastasis in OS by suppressing Wnt signaling. We used three miRNA target analysis tools to identify potential targets of miR-130b, and found that NKD2 is a potential target of miR-130b. Based ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.02.050

    authors： Li Z,Li Y,Wang N,Yang L,Zhao W,Zeng X

    更新日期：2016-03-18 00:00:00

  • Characterization of beta-carotene 15,15'-dioxygenase activity in TC7 clone of human intestinal cell line Caco-2.

    abstract：:The purpose of this study was to identify mammalian cell line(s) which possess intrinsic enzymatic activity of beta-carotene 15, 15'-dioxygenase. This enzyme (EC1.13.11.21) converts beta-carotene to retinal (precursor of retinol and retinoic acid). To assess activity, cellular enzyme preparations were incubated with b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9160

    authors： During A,Albaugh G,Smith JC Jr

    更新日期：1998-08-19 00:00:00