Nuclear localization signals in the core protein of hepatitis C virus.

abstract：:The ouabain-resistant and ouabain-sensitive alpha-subunit cRNAs in various molar ratios were injected into Xenopus oocytes together with cRNA for the beta-subunit. The ouabain-resistant ATPase activity, as well as ouabain-resistant Rb+ uptake, of the injected oocytes increased linearly with increasing the amount of cR...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Kawamura M

更新日期：2001-02-09 00:00:00

abstract：:Nitric oxide (NO*) is produced endogenously from NOS isoforms bound to sarcolemmal (SL) and sarcoplasmic reticulum (SR) membranes. To investigate whether locally generated NO* directly affects the activity of enzymes mediating ion active transport, we studied whether knockout of selected NOS isoforms would affect the ...

journal_title：Biochemical and biophysical research communications

authors： Zhou L,Burnett AL,Huang PL,Becker LC,Kuppusamy P,Kass DA,Kevin Donahue J,Proud D,Sham JS,Dawson TM,Xu KY

更新日期：2002-06-28 00:00:00

abstract：:CDX2 is an intestine-specific tumor suppressor gene encoding homeodomain-containing transcription factor, which is involved in a variety of developmental, proliferating, and differentiating processes. Moreover, the expression of CDX2 is reduced in a subset of primary colorectal cancers. In contrast, cyclooxygenase-2 (...

journal_title：Biochemical and biophysical research communications

authors： Kim SP,Park JW,Lee SH,Lim JH,Jang BC,Lee SH,Jang IH,Freund JN,Suh SI,Mun KC,Song DK,Ha EM,Lee WJ,Kwon TK

更新日期：2004-02-27 00:00:00

abstract：:While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purif...

journal_title：Biochemical and biophysical research communications

authors： Feizabadi MS,Jun Y

更新日期：2014-11-28 00:00:00

abstract：:The phosphonodifluoromethyl phenylalanine (F2Pmp) is superior to phosphonomethyl phenylalanine (Pmp) as a non-hydrolyzable phosphotyrosine (pTyr) mimetic. The difluoromethyl moiety increases the inhibitory potency of a F2Pmp-containing peptide over a Pmp-containing counterpart by 1000-fold toward the protein tyrosine ...

journal_title：Biochemical and biophysical research communications

authors： Chen L,Wu L,Otaka A,Smyth MS,Roller PP,Burke TR Jr,den Hertog J,Zhang ZY

更新日期：1995-11-22 00:00:00

abstract：:Oct4-Sox2-Nanog transcriptional networks are critical for the maintenance of embryonic stem (ES) cell self-renewal and induction of pluripotency. However, in transcription factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), Nanog is initially dispensable and Oct4 remains the sole...

journal_title：Biochemical and biophysical research communications

authors： Moon JH,Yun W,Kim J,Hyeon S,Kang PJ,Park G,Kim A,Oh S,Whang KY,Kim DW,Yoon BS,You S

更新日期：2013-02-15 00:00:00

abstract：:Using a monospecific antibody to the major cytosolic glutathione-S-transferase of human liver, we have isolated a cDNA clone from a human liver cDNA expression vector library in lambda gt11. The clone cross-hybridizes with a rat liver ligandin (glutathione-S-transferase 1-2) cDNA probe. The clone has an insert of 1.25...

journal_title：Biochemical and biophysical research communications

authors： Butera L,Monnier JR,Campbell E,Bhargava MM

更新日期：1987-02-13 00:00:00

abstract：:A human cell line established from a patient of an acute monocytic leukemia (THP-1) retained an ability to synthesize and secrete plasma apolipoprotein E like protein. The protein was identified with monospecific antibody raised against human plasma apolipoprotein E. The cells also secreted lipoprotein lipase (EC 3.1....

journal_title：Biochemical and biophysical research communications

authors： Tajima S,Hayashi R,Tsuchiya S,Miyake Y,Yamamoto A

更新日期：1985-01-16 00:00:00

abstract：:Deformation of vacuolar membranes mediated by endosomal sorting complex required for transport (ESCRT) is necessary for microautophagy. Target of rapamycin complex 1 (TORC1) protein kinase negatively regulates ESCRT-0 (Vps27-Hse1) recruitment onto vacuolar membranes and microautophagy induction. However, whether and h...

journal_title：Biochemical and biophysical research communications

authors： Sharmin T,Morshed S,Ushimaru T

更新日期：2020-04-09 00:00:00

abstract：:Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglyc...

journal_title：Biochemical and biophysical research communications

authors： Dhand R,Sharma VK,Teng AL,Krishnasamy S,Gross NJ

更新日期：1998-03-27 00:00:00

abstract：:We have extracted and purified proteins from calcium containing urinary stones with 4 M guanidine chloride and sequenced the amino acids of the proteins. The protein of approximately 49kDa was defined as human alpha-one antitrypsin (AT, from 23rd to 42nd amino acids of NH2-terminal) by amino acid sequence analysis. Th...

journal_title：Biochemical and biophysical research communications

authors： Umekawa T,Kohri K,Amasaki N,Yamate T,Yoshida K,Yamamoto K,Suzuki Y,Sinohara H,Kurita T

更新日期：1993-06-30 00:00:00

abstract：:In this study, a new skin-depigmenting agent, 2,6-dimethoxy-N-(4-methoxyphenyl)benzamide (DMPB), was synthesized using a combination of benzoic acid and aniline. DMPB exhibited significant depigmentation ability on the UV B-induced hyperpigmentation of the brown guinea pig skin. In addition, the 100ppm treatment with ...

journal_title：Biochemical and biophysical research communications

authors： Choi SY,Hwang JS,Kim S,Kim SY

更新日期：2006-10-13 00:00:00

abstract：:Class II transactivator (CIITA) plays a critical role in controlling major histocompatibility complex (MHC) class II gene expression. In this study, two novel alternatively spliced variants of human interferon (IFN)-γ-inducible CIITA, one missing exon 7 (CIITAΔE7), the other with TAG inserted at exon 4/5 junction (CII...

journal_title：Biochemical and biophysical research communications

authors： Chiu BL,Li CH,Chang CC

更新日期：2013-10-11 00:00:00

abstract：:In this investigation, the activities of five enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-dehydrogenase) involved in oxygen radical (OR)-detoxification and antioxidative defense were measured in conjunction with an index of lipid peroxidation in major prostatic lob...

journal_title：Biochemical and biophysical research communications

authors： Ghatak S,Ho SM

更新日期：1996-05-15 00:00:00

abstract：:Xenopus XmN-cadherin gene appears to have dual functions, since its mRNA is maternally provided in unfertilized eggs, once disappears almost completely during gastrula stage, then accumulates again specifically in neural tissues in later stage embryos. In the present experiment, we first followed the change in XmN-cad...

journal_title：Biochemical and biophysical research communications

authors： Hojyo T,Tooi O,Tashiro K,Shiokawa K

更新日期：1998-01-06 00:00:00

abstract：:The hereditary renal carcinoma (RC) in the rat, originally reported by R. Eker in 1954, is an excellent example of a Mendelian dominantly inherited predisposition to a specific cancer in an experimental animal. Recently, we have identified a germline mutation of the sclerosis (Tsc2) gene in the Eker rat (Nature Geneti...

journal_title：Biochemical and biophysical research communications

authors： Orimoto K,Tsuchiya H,Kobayashi T,Matsuda T,Hino O

更新日期：1996-02-06 00:00:00

abstract：:The aim of this work was to characterize folates modified by ONOO(-) and HOCl and to evaluate the binding capacity of folates modified by ONOO(-) to folate receptor alpha and beta. For the modification of folate by ONOO(-), folic acid was reacted with the combination of PMA activated PMN and PAPA NONOate or chemically...

journal_title：Biochemical and biophysical research communications

authors： Nakamura M,Nagayoshi R,Ijiri K,Nakashima-Matsushita N,Takeuchi T,Matsuyama T

更新日期：2002-10-11 00:00:00

abstract：:Chitin oligosaccharides elicited chitinase activity in melon plants. Hexamer to nonamer were the most efficient elicitors: hexamer for maximal stimulation of colorimetrically assessed chitinase activity, heptamer for maximal stimulation of radiochemically assessed chitinase activity. Chitinase elicitation was a rapid ...

journal_title：Biochemical and biophysical research communications

authors： Roby D,Gadelle A,Toppan A

更新日期：1987-03-30 00:00:00

abstract：:The ribonucleotide reductase from the strictly anaerobic methanogen Methanobacterium thermoautotrophicum has been partially purified by ion-exchange and gel-filtration chromatography. Its molecular weight is estimated to be 100,000 by the latter step. Unlike all previously studied ribonucleotide reductases, the enzyme...

journal_title：Biochemical and biophysical research communications

authors： Sze IS,McFarlan SC,Spormann A,Hogenkamp HP,Follmann H

更新日期：1992-04-30 00:00:00

abstract：:A hydrogenase from a new species of sulfate reducing bacterium has been isolated and characterized. In contrast to other hydrogenases isolated from Desulfovibrio, this enzyme is found in the cytoplasmic fraction rather than in the periplasm. The specific activity of the enzyme, as measured in the hydrogen evolution as...

journal_title：Biochemical and biophysical research communications

authors： Czechowski MH,He SH,Nacro M,DerVartanian DV,Peck HD Jr,LeGall J

更新日期：1984-12-28 00:00:00

abstract：:Lipid accumulation in pancreatic beta-cells during high-fat (HF) feeding may be involved in inducing a defective insulin secretion due to lipotoxicity. Hormone-sensitive lipase (HSL) is expressed and active in beta-cells, but its importance for islet dysfunction during the development of type 2 diabetes is not known. ...

journal_title：Biochemical and biophysical research communications

authors： Winzell MS,Holm C,Ahrén B

更新日期：2003-05-02 00:00:00

abstract：:Phospholipases A2 (PLA2) catalyze the hydrolytic cleavage of free fatty acids from the sn-2 OH-moiety of glycerophospholipids. These enzymes have a number of functions, from digestion to signaling and toxicity of several venoms. They have also been implicated in inflammation and are connected to diverse diseases, such...

journal_title：Biochemical and biophysical research communications

authors： Mahalka AK,Kinnunen PK

更新日期：2013-06-28 00:00:00

abstract：:Diabetic cardiomyopathy is an essential complication of diabetes and characterized by persistent diastolic dysfunction, leading to myocardial fibrosis. Oxidative stress and inflammation lead to cell damage and are implicated in many disease states. In our study, we evaluated the effects of toll-like receptor 6 (TLR6) ...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Zhang Y

更新日期：2016-04-29 00:00:00

abstract：:Several RNAs, including rRNAs, snRNAs, snoRNAs, and some mRNAs, are known to be localized at specific sites in a cell. Although methods have been established to visualize RNAs in a living cell, no large-scale visual screening of localized RNAs has been performed. In this study, we constructed a genomic library in whic...

journal_title：Biochemical and biophysical research communications

authors： Andoh T,Oshiro Y,Hayashi S,Takeo H,Tani T

更新日期：2006-12-29 00:00:00

abstract：:We have identified a genomic DNA fragment, using the PCR method with degenerate oligonucleotide primers which contain the conserved sequence of the RING finger domain. Using the DNA fragment as a probe, a novel cDNA was cloned from human and mouse testis. The cDNA had a domain structure of the typical RING-B box-coile...

journal_title：Biochemical and biophysical research communications

authors： Orimo A,Yamagishi T,Tominaga N,Yamauchi Y,Hishinuma T,Okada K,Suzuki M,Sato M,Nogi Y,Suzuki H,Inoue S,Yoshimura K,Shimizu Y,Muramatsu M

更新日期：2000-09-16 00:00:00

abstract：:Mutations in the WNK1 and WNK4 genes result in an inherited hypertensive disease, pseudohypoaldosteronism type II (PHAII). Recently, the KLHL3 and Cullin3 genes were also identified as responsible genes for PHAII. Although we have reported that WNK4 is a substrate for the KLHL3-Cullin3 E3 ligase complex, it is not cle...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Mori T,Wakabayashi M,Mori Y,Susa K,Zeniya M,Sohara E,Rai T,Sasaki S,Uchida S

更新日期：2013-08-02 00:00:00

abstract：:Five commonly used cationic liposome formulations were tested for their ability to deliver DNA to established subcutaneous human tumor xenografts in SCID mice. Liposomes were complexed with a mammalian expression plasmid containing the bacterial beta-galactosidase gene and delivered to tumors by direct injection. The ...

journal_title：Biochemical and biophysical research communications

authors： Egilmez NK,Iwanuma Y,Bankert RB

更新日期：1996-04-05 00:00:00

abstract：:Using isolated liver mitochondria we show that low concentrations of TBT (0.5 microM) cause the release of mitochondrial cytochrome c, in the presence of Ca(2+). This is reflected in a rapid loss of membrane potential (DeltaPsi(m)), and a large-amplitude swelling characteristic of mitochondrial permeability transition...

journal_title：Biochemical and biophysical research communications

authors： Gogvadze V,Stridh H,Orrenius S,Cotgreave I

更新日期：2002-04-12 00:00:00

abstract：:Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation. Microglia surrounding the subventr...

journal_title：Biochemical and biophysical research communications

authors： Yamamiya M,Tanabe S,Muramatsu R

更新日期：2019-06-11 00:00:00

abstract：:Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 A. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. Thes...

journal_title：Biochemical and biophysical research communications

authors： Malicka J,Gryczynski I,Kusba J,Shen Y,Lakowicz JR

更新日期：2002-06-21 00:00:00