Abstract:
:Dengue fever is a growing public health concern around the world and despite vaccine development efforts, there are currently no effective dengue vaccines. In the present study we report the induction of protective antibodies against dengue virus by DNA immunization with domain III (DIII) region of the envelope protein (E) in a mouse model. The DIII region of all four dengue virus serotypes were cloned separately into pcDNA 3 plasmid. Protein expression was tested in COS-7 cells. Each plasmid, or a tetravalent combination, were used to immunize BALB/c mice by intramuscular route. Presence of specific antibodies was evaluated by ELISA, and neutralizing antibodies were tested using a cytopathogenic effect (CPE) inhibition assay in BHK-21 cells, as well as in newborn mice challenged intracranially with dengue 2 virus. Mice immunized with individual DIII constructs or the tetravalent formulation developed antibodies against each corresponding dengue serotype. Antibody titers by ELISA were similar for all serotypes and no significant differences were observed when boosters were administered, although antibody responses were dose-dependent. CPE inhibition assays using Den-2 virus showed neutralization titers of 1:10 in mice immunized with individual DIII plasmid or those immunized with the tetravalent formulations. 43% of newborn mice challenged with Den-2 in combination with sera from mice immunized with Den-2 DIII plasmid were protected, whereas sera from mice immunized with the tetravalent formulation conferred 87% protection. Our results suggest that DIII can be used as a tetravalent DNA formulation to induce neutralizing and protective antibodies against dengue virus.
journal_name
Vaccinejournal_title
Vaccineauthors
Mota J,Acosta M,Argotte R,Figueroa R,Méndez A,Ramos Cdoi
10.1016/j.vaccine.2004.12.028subject
Has Abstractpub_date
2005-05-16 00:00:00pages
3469-76issue
26eissn
0264-410Xissn
1873-2518pii
S0264-410X(05)00204-5journal_volume
23pub_type
杂志文章相关文献
VACCINE文献大全abstract::In this study, a recombinant truncated West Nile virus envelope protein antigen (rWNV-E) was produced in serum-free cultures of the expresSF+ insect cell line via baculovirus infection. This production system was selected based on its use in the production of candidate human and animal vaccine antigens. A defined ferm...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.10.046
更新日期:2009-01-07 00:00:00
abstract::Ethnicity may be associated with the incidence of pneumococcal infections and the frequency of protective vaccine responses. Earlier studies have suggested that HIV-infected persons of black ethnicity develop less robust immune responses to pneumococcal vaccination that may relate to their higher incidence of invasive...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.056
更新日期:2010-11-10 00:00:00
abstract::Safe RSV vaccine development has challenged the medical community since a formalin-killed RSV vaccine caused disease exacerbation in the 1960s. Disease was replicated using the bovine RSV system in one of two studies. The studies differed in viral protein dose and length of time between vaccination and infection. Dise...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.10.024
更新日期:2004-03-29 00:00:00
abstract::We demonstrated the feasibility of collecting and disseminating mid-season estimates of influenza vaccine coverage. We surveyed a nationally representative sample of U.S. adults (n = 3972) via the Internet about use of influenza vaccination as of mid-November 2008. Findings were presented on 10 December 2008 to the me...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.04.007
更新日期:2009-06-08 00:00:00
abstract::In clinical trials recombinant-modified vaccinia virus Ankara expressing the Mycobacterium tuberculosis antigen 85A (MVA85A) induces approximately 10 times more effector T cells than any other recombinant MVA vaccine. We have found that in BCG primed subjects MVA85A vaccination reduces transforming growth factor beta ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.07.040
更新日期:2008-09-26 00:00:00
abstract::Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), is an important human pathogen. Bacillus Calmette-Guérin (BCG), a live, attenuated variant of Mycobacterium bovis, is currently the only available TB vaccine despite its low efficacy against the infectious pulmonary form of the disease in adults. ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.10.124
更新日期:2015-12-16 00:00:00
abstract:BACKGROUND:Although pregnant women are the highest priority group for seasonal influenza vaccination, maternal influenza vaccination rates remain suboptimal. The purpose of this study was to evaluate the effect of a brief education intervention on maternal influenza vaccine uptake. METHODS:During the 2013-14 and 2014-...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.vaccine.2016.09.019
更新日期:2016-10-17 00:00:00
abstract:BACKGROUND:Pregnant women are at risk of severe influenza disease and are a priority group for influenza vaccination programs. Nicaragua expanded recommendations to include influenza vaccination to all pregnant women in the municipality of Managua in 2013. METHODS:We carried out a survey among 1,807 pregnant women who...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.065
更新日期:2016-02-17 00:00:00
abstract::The long-term duration of recombinant hepatitis B vaccine-induced immunity among persons vaccinated starting at birth is still not well understood. Waning of vaccine-induced immunity could leave young adults at risk of hepatitis B virus infection due to behavioral or occupational exposures. We followed a cohort of chi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.12.106
更新日期:2012-02-21 00:00:00
abstract::Respiratory syncytial virus (RSV) is the most common cause of viral bronchiolitis and pneumonia in children. The present study compares the level of attenuation, genetic stability and efficacy of three conditional-lethal temperature-sensitive (ts) mutants of the RSV A2 wild-type virus, designated ts-1, ts-1-NG1, and t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90168-w
更新日期:1993-11-01 00:00:00
abstract:CONTEXT:The true level of influenza vaccine efficacy is controversial and many factors may influence its estimation. OBJECTIVES:To estimate the efficacy of vaccination of children and non-elderly adults for the prevention of influenza and to explore the impact of type of vaccine, age, degree of strain matching, influe...
journal_title:Vaccine
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.vaccine.2012.10.084
更新日期:2012-12-17 00:00:00
abstract::Dengue fever and dengue hemorrhagic fever are endemic throughout tropical and subtropical countries. Four serotypes of dengue viruses (DENV-1 to DENV-4), each with several genotypes including various subclades, are co-distributed in most endemic areas. Infection-neutralizing and -enhancing antibodies are believed to p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.06.017
更新日期:2014-07-23 00:00:00
abstract::Respiratory syncytial virus F-protein (RSV-F) is poorly expressed from DNA expression plasmids containing the wild type RSV-F open reading frame. By codon optimization, premature polyadenylation signals were deleted and a striking enhancement of RSV-F expression levels was achieved. Therefore, the immunogenicity and e...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.07.025
更新日期:2007-10-10 00:00:00
abstract::Tuberculosis caused by Mycobacterium tuberculosis continues to be one of the major public health problems in the world. The eventual control of this disease will require the development of a safe and effective vaccine. One of the approaches receiving a great deal of attention recently is subunit vaccination. An effica...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2006.11.014
更新日期:2007-02-19 00:00:00
abstract::Vaccine induced responses are often weaker in those individuals most susceptible to infection, namely the very young and the elderly, highlighting the need for safe and effective vaccine adjuvants. Herein we evaluated different archaeosome formulations as an adjuvant to the H1N1 influenza hemagglutinin protein and com...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.07.010
更新日期:2019-11-08 00:00:00
abstract::The IgG isotype response in Balb/c mice infected with FMDV or immunized with different vaccine formulations using inactivated virus particles as antigen was analyzed at various times post-inoculation. For this purpose an ELISA based on polyclonal antibodies for detection and quantification of mouse IgG isotypes with F...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)00078-f
更新日期:1995-07-01 00:00:00
abstract::The prospective studies available today confirm the experience gained from several retrospective studies that TBE is a disease with a severe acute clinical course and considerable long-term morbidity. A defined post-encephalitic TBE syndrome exists, causing long-lasting morbidity that often affects the quality of life...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(02)00811-3
更新日期:2003-04-01 00:00:00
abstract::A bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. So cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrh...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.09.023
更新日期:2008-11-11 00:00:00
abstract::Induction of immune response as characterised by expression of cytokine genes in the spleen following immunization of pre- and post-hatch chickens with herpesvirus of turkeys (HVT) vaccine was studied. The pattern of expression of IFN-gamma and IL-10 genes in pre-hatch immunized chickens was different from that observ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.069
更新日期:2008-05-02 00:00:00
abstract::The annual winter outbreak of influenza is one of the major cause of morbidity and mortality among frail elderly people. The aim of the present study was to identify prevalence and predictors of influenza vaccination in a large European population of frail and old people living in community. This was an observational ...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2005.03.008
更新日期:2005-06-10 00:00:00
abstract::Chagas' disease is a major tropical disease for which a cure for chronic phase does not exist yet. Trypanosoma cruzi trans-sialidase (TS) seems to be involved in relevant processes such as infectivity, host survival and, very importantly, disease pathogenesis. In this study, we show that mice vaccinated with an engine...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.060
更新日期:2008-05-02 00:00:00
abstract::Nasal mucosal barrier is a key impediment for the absorption of influenza whole inactivated virus (WIV) intranasal vaccine. Yet it is still unclear how WIV cross the epithelial cells (ECs) in nasal cavity. Here, in vitro, a coculture system was well established, consisting of surrogate nasal ECs (Calu-3) and dendritic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.022
更新日期:2015-03-10 00:00:00
abstract:BACKGROUND:Maternal immunization with tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis vaccine (Tdap) has recently been implemented to prevent infant pertussis. Tdap is still not routinely recommended in Thailand, and there are limited data to support or challenge this strategy. OBJECTIVES:The primar...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.01.074
更新日期:2020-03-10 00:00:00
abstract::In an agent-based computer model, we simulate the formation and recall of anti-virus immunological memory. Specifically we try to predict what will happen, both to the response and to memory, when the second infecting virus is partly different from the first one, and when the cross-reactivity of the two branches of th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.11.109
更新日期:2009-02-05 00:00:00
abstract::The hepatitis B virus (HBV) has infected more than 2000 million persons alive today and 350 million persons are chronically infected carriers of the virus, at high risk of death from active hepatitis, cirrhosis and primary hepatocellular cancer. Each year approximately 1 million people die from the acute and chronic s...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)80050-n
更新日期:1995-01-01 00:00:00
abstract::Haemagglutinin (HA), a mixture of haemagglutinin and neuraminidase (HA + NA), and matrix (M) protein were isolated from the influenza A virus and covalently coupled to a synthetic polyelectrolyte (P). A single injection into mice of the resultant conjugates (virogates) brought about efficient stimulation of the primar...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(85)90130-6
更新日期:1985-12-01 00:00:00
abstract::A mass vaccination campaign against influenza A/H1N1 was launched in France in October 2009. Vaccination was offered free of charge to the entire population according to a pre-defined order of priority. Demographic data and data on vaccinations given were recorded in a dedicated database. We analysed vaccine uptake by...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.096
更新日期:2010-11-29 00:00:00
abstract::Clinical trials with pandemic influenza vaccine candidates have focused on aluminium hydroxide as an adjuvant to boost humoral immune responses. In this study we investigated the effect of aluminium hydroxide on the magnitude and type of immune response induced by whole-inactivated virus (WIV) vaccine. Balb/c mice wer...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.063
更新日期:2008-05-02 00:00:00
abstract:OBJECTIVE:To estimate current age-specific rates of immunity to hepatitis A virus (HAV) in world regions by conducting a systematic review and meta-analysis of published data. The estimation of the global burden of hepatitis A and policies for public health control are dependent on an understanding of the changing epid...
journal_title:Vaccine
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.vaccine.2010.08.037
更新日期:2010-09-24 00:00:00
abstract::Brucellosis is one of the most prevalent zoonotic diseases of worldwide distribution caused by the infection of genus Brucella. Live attenuated vaccines such as B. abortus S19, B. abortus RB51 and B. melitensis Rev1 are found most effective against brucellosis infection in animals, contriving a number of serious side ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.07.106
更新日期:2014-09-29 00:00:00