A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells.


:In this study, a recombinant truncated West Nile virus envelope protein antigen (rWNV-E) was produced in serum-free cultures of the expresSF+ insect cell line via baculovirus infection. This production system was selected based on its use in the production of candidate human and animal vaccine antigens. A defined fermentation and purification process for the rWNV-E antigen was established to control for purity and immunogenicity of each protein batch. The material formulated with aluminum hydroxide was stable for greater than 8months at 4 degrees C. The recombinant vaccine candidate was evaluated for immunogenicity and protective efficacy in several animal models. In mouse and hamster WNV challenge models, the vaccine candidate induced viral protection that correlated with anti-rWNV-E immunogenicity and WNV neutralizing antibody titers. The rWNV-E vaccine candidate was used to boost horses previously immunized with the Fort Dodge inactivated WNV vaccine and also to induce WNV neutralizing titers in naïve foals that were at least 14weeks of age. Furthermore, the vaccine candidate was found safe when high doses were injected into rats, with no detectable treatment-related clinical adverse effects. These observations demonstrate that baculovirus-produced rWNV-E can be formulated with aluminum hydroxide to produce a stable and safe vaccine which induces humoral immunity that can protect against WNV infection.






Bonafé N,Rininger JA,Chubet RG,Foellmer HG,Fader S,Anderson JF,Bushmich SL,Anthony K,Ledizet M,Fikrig E,Koski RA,Kaplan P




Has Abstract


2009-01-07 00:00:00
















  • Knowledge, attitudes and beliefs related to seasonal influenza vaccine among pregnant women in Thailand.

    abstract:BACKGROUND:In 2009, Thailand recommended pregnant women be prioritized for influenza vaccination. Vaccine uptake among Thai pregnant women is lower than other high-risk groups. METHODS:During December 2012-April 2013, we conducted a cross-sectional survey of a convenience sample of Thai pregnant women aged ≥ 15 years ...


    pub_type: 杂志文章


    authors: Ditsungnoen D,Greenbaum A,Praphasiri P,Dawood FS,Thompson MG,Yoocharoen P,Lindblade KA,Olsen SJ,Muangchana C

    更新日期:2016-04-19 00:00:00

  • Immunogenicity, reactogenicity and persistence of meningococcal A, C, W-135 and Y-tetanus toxoid candidate conjugate (MenACWY-TT) vaccine formulations in adolescents aged 15-25 years.

    abstract::Development of meningococcal serogroups A, C, W-135 and Y conjugate vaccines could expand coverage against devastating meningococcal diseases. The immunogenicity of one dose of each one of five MenACWY-TT formulations versus a licensed ACWY polysaccharide vaccine was evaluated in 175 healthy subjects of 15-25 years. S...


    pub_type: 杂志文章,随机对照试验


    authors: Ostergaard L,Lebacq E,Poolman J,Maechler G,Boutriau D

    更新日期:2009-01-01 00:00:00

  • Evaluation of 12 stabilizers in a developed attenuated Salmonella Enteritidis vaccine.

    abstract::The development of a stable live attenuated Salmonella Enteritidis (SE) vaccine, resisting heat stress during transportation and storage in unequipped tropical and subtropical zones of the world, is highly recommended. Twelve stabilizers were individually supplemented into a 9 ml volume of sterile distilled water resu...


    pub_type: 杂志文章


    authors: Barbour EK,Abdelnour A,Jirjis F,Faroon O,Farran MT

    更新日期:2002-05-22 00:00:00

  • Analysis of efficacy of CVD 103-HgR live oral cholera vaccine against all-cause travellers' diarrhoea in a randomised, double-blind, placebo-controlled study.

    abstract::Enterotoxigenic Escherichia coli (ETEC), which produces heat labile toxin (LT) and/or heat stable toxin (ST), is considered to be the most common known cause of travellers' diarrhoea (TD). Owing to the antigenic similarity between cholera toxin and LT, immunization with inactivated oral B-subunit/whole-cell cholera va...


    pub_type: 杂志文章,随机对照试验


    authors: Leyten EM,Soonawala D,Schultsz C,Herzog C,Ligthelm RJ,Wijnands S,Visser LG

    更新日期:2005-10-17 00:00:00

  • Retrospective evaluation of foot-and-mouth disease vaccine effectiveness in Turkey.

    abstract::Foot-and-mouth disease (FMD) is present in much of Turkey and its control is largely based on vaccination. The arrival of the FMD Asia-1 serotype in Turkey in 2011 caused particular concern, spreading rapidly westwards across the country towards the FMD free European Union. With no prior natural immunity, control of s...


    pub_type: 杂志文章


    authors: Knight-Jones TJ,Bulut AN,Gubbins S,Stärk KD,Pfeiffer DU,Sumption KJ,Paton DJ

    更新日期:2014-04-01 00:00:00

  • Effectiveness of the 2010-2011 seasonal influenza vaccine in preventing confirmed influenza hospitalizations in adults: a case-case comparison, case-control study.

    abstract:INTRODUCTION:We estimated influenza vaccine effectiveness (IVE) to prevent laboratory-confirmed influenza-related hospitalizations in patients 18 years old or older during the 2010-2011 influenza season. METHODS:We conducted a prospective case-control study in five hospitals, in Valencia, Spain. Study subjects were co...


    pub_type: 杂志文章,多中心研究


    authors: Puig-Barberà J,Díez-Domingo J,Arnedo-Pena A,Ruiz-García M,Pérez-Vilar S,Micó-Esparza JL,Belenguer-Varea A,Carratalá-Munuera C,Gil-Guillén V,Schwarz-Chavarri H

    更新日期:2012-08-24 00:00:00

  • A recombinant hybrid outer membrane protein for vaccination against Pseudomonas aeruginosa.

    abstract::Among the numerous targets which can be used for the development of vaccines against Pseudomonas aeruginosa we focused on the outer membrane proteins OprF and OprI. The C-terminal part of OprF from aa 190 to aa 350 was investigated for its conservation and its localization of B-cell epitopes. A hybrid protein which co...


    pub_type: 杂志文章


    authors: Knapp B,Hundt E,Lenz U,Hungerer KD,Gabelsberger J,Domdey H,Mansouri E,Li Y,von Specht BU

    更新日期:1999-03-26 00:00:00

  • Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters.

    abstract::We demonstrated earlier that immunization with recombinant Leptospira immunoglobulin like protein A (LigA) induced significant protection against virulent Leptospira interrogans serovar Pomona challenge in hamsters. However, the protective immune mechanism remains unclear. In the present study we demonstrated the prot...


    pub_type: 杂志文章


    authors: Faisal SM,Yan W,Chen CS,Palaniappan RU,McDonough SP,Chang YF

    更新日期:2008-01-10 00:00:00

  • Soluble recombinant human metapneumovirus G protein is immunogenic but not protective.

    abstract::Human metapneumovirus (HMPV) expresses the major surface glycoproteins F and G. We evaluated the protective efficacy of immunization with G. We generated a recombinant form of G ectodomain (GDeltaTM) that was secreted from mammalian cells and purified by affinity chromatography. We tested the immunogenicity of GDeltaT...


    pub_type: 杂志文章


    authors: Ryder AB,Tollefson SJ,Podsiad AB,Johnson JE,Williams JV

    更新日期:2010-06-07 00:00:00

  • rOmpF and OMVs as efficient subunit vaccines against Salmonella enterica serovar Enteritidis infections in poultry farms.

    abstract::Salmonella enterica serovar Enteritidis remains the most prevalent serotype causing human salmonellosis through the consumption of contaminated foods, especially poultry products. The development of a subunit vaccine against S. Enteritidis can not only protect chickens against Salmonella infection in the poultry indus...


    pub_type: 杂志文章


    authors: Li Q,Ren J,Xian H,Yin C,Yuan Y,Li Y,Ji R,Chu C,Qiao Z,Jiao X

    更新日期:2020-10-21 00:00:00

  • Nature, evolution, and appraisal of adverse events and antibody response associated with the fifth consecutive dose of a five-component acellular pertussis-based combination vaccine.

    abstract::We performed a randomized, controlled clinical trial to characterize the evolution of the adverse events associated with the fifth consecutive dose of an acellular pertussis vaccine, and to assess the level of discomfort associated with the injection and the attitude of parents concerning these events. A total of 505 ...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Halperin SA,Scheifele D,Mills E,Guasparini R,Humphreys G,Barreto L,Smith B

    更新日期:2003-06-02 00:00:00

  • Current approaches to the development of vaccines against disease caused by respiratory syncytial virus (RSV) and parainfluenza virus (PIV). A meeting report of the WHO Programme for Vaccine Development.

    abstract::The paramyxoviruses respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV3) are the two most common agents of severe lower respiratory tract disease in infants and children throughout the world. RSV causes yearly epidemics of bronchiolitis and pneumonia in infants and young children, while PIV3 is a co...




    authors: Crowe JE Jr

    更新日期:1995-03-01 00:00:00

  • Use of a booster dose of capsular group C meningococcal glycoconjugate vaccine to demonstrate immunologic memory in children primed with one or two vaccine doses in infancy.

    abstract:BACKGROUND:Use of a polysaccharide vaccine challenge to demonstrate immunologic memory after priming with capsular group C meningococcal conjugate vaccines (MenCC) risks induction of immunologic hyporesponsiveness. For this reason, MenCC vaccines are now used as probes of immunologic memory, however, no studies have de...


    pub_type: 杂志文章,随机对照试验


    authors: Pace D,Khatami A,Attard-Montalto S,Voysey M,Finn A,Faust SN,Heath PT,Borrow R,Snape MD,Pollard AJ

    更新日期:2016-12-07 00:00:00

  • Local and regional adverse reactions to BCG-SSI vaccination: a 12-month cohort follow-up study.

    abstract::In a 12-month cohort follow-up study of 2435 children vaccinated in 2007 by Statens Serum Institute BCG strain (BCG SSI, 17.8% had an adverse event (AE): erythema 12.4%, induration 12.2%, abscesses 2.5%, ulceration 0.9%, lymphadenitis 0.1%. The factors associated with a lower risk of AE were: age at vaccination <1 yea...


    pub_type: 杂志文章,评审


    authors: Dommergues MA,de La Rocque F,Guy C,Lécuyer A,Jacquet A,Guérin N,Fagot JP,Boucherat M,d'Athis P,Cohen R

    更新日期:2009-11-23 00:00:00

  • Iscom and iscom-matrix enhance by intranasal route the IgA responses to OVA and rCTB in local and remote mucosal secretions.

    abstract::Iscoms, with rCTB incorporated via the GM1 receptor, enhanced in mice the mucosal immunogenicity of rCTB as antigen after intranasal (i.n.) administration both by inducing IgA response in the remote intestinal tract mucosa and by a 100-fold increase of the specific IgA locally in the lungs. Iscom-matrix as a separate ...


    pub_type: 杂志文章


    authors: Ekström J,Hu KF,Bengtsson KL,Morein B

    更新日期:1999-06-04 00:00:00

  • The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.

    abstract::Here we demonstrated that bis-(3',5')-cyclic dimeric inosine monophosphate (c-di-IMP) exhibits potent adjuvant properties. BALB/c or C57BL/6 mice were immunized with the model antigens beta-galactosidase (beta-Gal) or Ovalbumin (OVA) alone or co-administered with c-di-IMP by the intranasal route. Animals receiving c-d...


    pub_type: 杂志文章


    authors: Libanova R,Ebensen T,Schulze K,Bruhn D,Nörder M,Yevsa T,Morr M,Guzmán CA

    更新日期:2010-03-02 00:00:00

  • What maintains parental support for vaccination when challenged by anti-vaccination messages? A qualitative study.

    abstract::This study sought to explore how parents respond to competing media messages about vaccine safety. Six focus groups with mothers of infants were shown television vignettes of typical pro- and anti-vaccination claims. Thematic analysis of transcripts was undertaken. Mothers expressed surprise and concern about alleged ...


    pub_type: 杂志文章


    authors: Leask J,Chapman S,Hawe P,Burgess M

    更新日期:2006-11-30 00:00:00

  • Boosting immune response with a candidate varicella-zoster virus glycoprotein subunit vaccine.

    abstract::A varicella-zoster virus (VZV) seropositive individual was immunized with 100 micrograms of purified VZV TgpI-511 glycoprotein subunit antigen formulated with monophosphoryl lipid A. Serum samples were obtained during a 40-day period post-immunization (PI) and analysed by immunoprecipitation and virus neutralization t...


    pub_type: 杂志文章


    authors: Vafai A

    更新日期:1995-10-01 00:00:00

  • The epidemiology and burden of rotavirus in China: a review of the literature from 1983 to 2005.

    abstract::We reviewed studies of rotavirus in MEDLINE and the Chinese literature to get a preliminary estimate of the burden of rotavirus gastroenteritis in China and the epidemiology of the disease. Studies were selected if they were conducted for a period 1 year or more, had more than 100 patients enrolled, and used an accept...


    pub_type: 杂志文章


    authors: Orenstein EW,Fang ZY,Xu J,Liu C,Shen K,Qian Y,Jiang B,Kilgore PE,Glass RI

    更新日期:2007-01-05 00:00:00

  • A combination vaccine confers full protection against co-infections with influenza, herpes simplex and respiratory syncytial viruses.

    abstract::Combined/composite vaccines should be useful in reducing the number of vaccinations and provide more flexibility in confronting biological warfare scenarios. We tested the effectiveness of a composite genetic vaccine designed from previously known protective antigens directed against influenza A virus (INF-A), herpes ...


    pub_type: 杂志文章


    authors: Talaat AM,Lyons R,Johnston SA

    更新日期:2001-11-12 00:00:00

  • Oral immunization with a Streptococcal pneumoniae polysaccharide conjugate vaccine in enterocoated microparticles induces serum antibodies against type specific polysaccharides.

    abstract::The authors examined the antibody responses of mice orally immunized with pneumococcal polysaccharide (type 23F) or a pneumococcal polysaccharide conjugated to the outer membrane protein complex of Neisseria meningitides (23F-OMPC). These antigens were administered either in solution or entrapped within microcapsules....


    pub_type: 杂志文章


    authors: Flanagan MP,Michael JG

    更新日期:1999-01-01 00:00:00

  • Technological investigations with attenuated strains of Shigella for production of live vaccines. 1. Cultivation in a fermenter of an attenuated strain S. flexneri 2a with two markers.

    abstract::A method was developed for cultivation of the strain Shigella flexneri 2a 77 with two attenuated markers. It ensures the preservation of the initial properties of the strain. The regimen for control of dissolved oxygen level was optimized. It was established that yeast extract is a necessary component of the nutritive...


    pub_type: 杂志文章


    authors: Nenkov P,Bratoeva M,Vitanov T,Marinova S,Denchev V,Linde K

    更新日期:1987-09-01 00:00:00

  • An alternative signal 3: CD8⁺ T cell memory independent of IL-12 and type I IFN is dependent on CD27/OX40 signaling.

    abstract::Type I IFN and IL-12 are well documented to serve as so called "signal 3" cytokines, capable of facilitating CD8(+) T cell proliferation, effector function and memory formation. While their ability to serve in this capacity is well established, to date, no non-cytokine signal 3 mediators have been clearly identified. ...


    pub_type: 杂志文章


    authors: Sanchez PJ,Kedl RM

    更新日期:2012-02-01 00:00:00

  • Pandemic H1N1 influenza virus-like particles are immunogenic and provide protective immunity to pigs.

    abstract::The outbreak of the 2009 influenza pandemic underscored the important role of swine in influenza virus evolution and the emergence of novel viruses with pandemic potential. Vaccination is the most common practice to control swine influenza in swine industry. Influenza virus-like particle (VLP) vaccines are an alternat...


    pub_type: 杂志文章


    authors: Pyo HM,Masic A,Woldeab N,Embury-Hyatt C,Lin L,Shin YK,Song JY,Babiuk S,Zhou Y

    更新日期:2012-02-08 00:00:00

  • Comparing pneumococcal conjugate vaccine schedules based on 3 and 2 primary doses: systematic review and meta-analysis.

    abstract:BACKGROUND:Pneumococcal conjugate vaccines (PCV) were first licensed for use with 3 primary doses in infancy and a booster dose. The evidence for the effects of different schedules was examined in this systematic review and meta-analysis. METHODS:We searched 12 databases and trial registers up to March 2010. We select...


    pub_type: 杂志文章,meta分析,评审


    authors: Scott P,Rutjes AW,Bermetz L,Robert N,Scott S,Lourenço T,Egger M,Low N

    更新日期:2011-12-06 00:00:00

  • Immune response to a Tdap booster in vertically HIV-infected adolescents.

    abstract:BACKGROUND:Pertussis cases have increased worldwide and knowledge on immune response and cytokine profile after Tdap vaccine in immunodeficient adolescents is scarce. OBJECTIVE:To evaluate the immune response after Tdap in HIV-infected (HIV) and in healthy adolescents (CONTROL). METHODOLOGY:Thirty HIV adolescents wit...


    pub_type: 杂志文章


    authors: Spina FG,Gouvea A,Succi RCM,Calanca F,Weckx LY,Terreri MT,Takano MAS,de Moraes-Pinto MI

    更新日期:2018-09-05 00:00:00

  • Implementation of good clinical practice guidelines in vaccine trials in developing countries.

    abstract::The practicalities when applying the ICH GCPs (International Conference on Harmonization 1996 Good Clinical Practices [EU, MHLW, FDA. International Conference on Harmonization Guideline for Good Clinical Practice; 1997] in less developed countries (ldcs) are seldom discussed and we found no guidelines as how to "adapt...


    pub_type: 杂志文章


    authors: Acosta CJ,Galindo CM,Ochiai RL,Danovaro-Holliday MC,Laure-Page A,Thiem VD,Jin Y,Khan MI,Sahito SM,Hamza HB,Park JK,Lee H,Bock H,Elyazeed RA,Albert MJ,Ascaso C,Robles TQ,Ali M,Ngai P,Puri MK,Koo YM,Agtini MD,So

    更新日期:2007-04-12 00:00:00

  • Protection induced in mice against a lethal orthopox virus by the Lister strain of vaccinia virus and modified vaccinia virus Ankara (MVA).

    abstract::The Lister (Elstree) strain of vaccinia virus, used in Israel for vaccination against smallpox, was studied in tissue cultures and in a mouse model. The virus failed to reach the brain of the mice when inoculated intranasally at a dose of 500,000 plaque forming units, but was lethal for 50% of them, when injected intr...


    pub_type: 杂志文章


    authors: Abdalrhman I,Gurt I,Katz E

    更新日期:2006-05-08 00:00:00

  • Potentiation of an anthrax DNA vaccine with electroporation.

    abstract::DNA vaccines are a promising method of immunization against biothreats and emerging infections because they are relatively easy to design, manufacture, store and distribute. However, immunization with DNA vaccines using conventional delivery methods often fails to induce consistent, robust immune responses, especially...


    pub_type: 杂志文章


    authors: Luxembourg A,Hannaman D,Nolan E,Ellefsen B,Nakamura G,Chau L,Tellez O,Little S,Bernard R

    更新日期:2008-09-19 00:00:00

  • Complete protection of cats against feline panleukopenia virus challenge by a recombinant canine adenovirus type 2 expressing VP2 from FPV.

    abstract::Feline panleukopenia virus (FPV) is an important infectious pathogen of all members of the family Felidae. Here, we describe construction of a replication-competent recombinant canine adenovirus type 2 (CAV-2) expressing the VP2 protein of FPV (CAV-2-VP2) by transfection of MDCK cells with recombinant CAV-2 genome car...


    pub_type: 杂志文章


    authors: Yang S,Xia X,Qiao J,Liu Q,Chang S,Xie Z,Ju H,Zou X,Gao Y

    更新日期:2008-03-10 00:00:00