Abstract：
:In this study, a recombinant truncated West Nile virus envelope protein antigen (rWNV-E) was produced in serum-free cultures of the expresSF+ insect cell line via baculovirus infection. This production system was selected based on its use in the production of candidate human and animal vaccine antigens. A defined fermentation and purification process for the rWNV-E antigen was established to control for purity and immunogenicity of each protein batch. The material formulated with aluminum hydroxide was stable for greater than 8months at 4 degrees C. The recombinant vaccine candidate was evaluated for immunogenicity and protective efficacy in several animal models. In mouse and hamster WNV challenge models, the vaccine candidate induced viral protection that correlated with anti-rWNV-E immunogenicity and WNV neutralizing antibody titers. The rWNV-E vaccine candidate was used to boost horses previously immunized with the Fort Dodge inactivated WNV vaccine and also to induce WNV neutralizing titers in naïve foals that were at least 14weeks of age. Furthermore, the vaccine candidate was found safe when high doses were injected into rats, with no detectable treatment-related clinical adverse effects. These observations demonstrate that baculovirus-produced rWNV-E can be formulated with aluminum hydroxide to produce a stable and safe vaccine which induces humoral immunity that can protect against WNV infection.
journal_name
Vaccinejournal_title
Vaccineauthors
Bonafé N,Rininger JA,Chubet RG,Foellmer HG,Fader S,Anderson JF,Bushmich SL,Anthony K,Ledizet M,Fikrig E,Koski RA,Kaplan Pdoi
10.1016/j.vaccine.2008.10.046subject
Has Abstractpub_date
2009-01-07 00:00:00pages
213-22issue
2eissn
0264-410Xissn
1873-2518pii
S0264-410X(08)01450-3journal_volume
27pub_type
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