MAP kinase phosphorylation of plant profilin.


:Profilin is a small actin-binding protein and is expressed at high levels in mature pollen where it is thought to regulate actin filament dynamics upon pollen germination and tube growth. The majority of identified plant profilins contain a MAP kinase phosphorylation motif, P-X-T-P, and a MAP kinase interaction motif (KIM). In in vitro kinase assays, the tobacco MAP kinases p45(Ntf4) and SIPK, when activated by the tobacco MAP kinase kinase NtMEK2, can phosphorylate the tobacco profilin NtProf2. Mutagenesis of the threonine residue in this motif identified it as the site of MAP kinase phosphorylation. Fractionation of tobacco pollen extracts showed that p45(Ntf4) is found exclusively in the high-speed pellet fraction while SIPK and profilin are predominantly cytosolic. These data identify one of the first substrates to be directly phosphorylated by MAP kinases in plants.


Limmongkon A,Giuliani C,Valenta R,Mittermann I,Heberle-Bors E,Wilson C




Has Abstract


2004-11-05 00:00:00














  • Some fused heterocyclic compounds as eukaryotic topoisomerase II inhibitors.

    abstract::Our previously synthesized 37 compounds, which are 2,5,6-substituted benzoxazole, benzimidazole, benzothiazole, and oxazolo(4,5-b)pyridine derivatives, were tested for their eukaryotic DNA topoisomerase II inhibitory activity in cell free system and 28 were found to inhibit the topoisomerase II at an initial concentra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pinar A,Yurdakul P,Yildiz I,Temiz-Arpaci O,Acan NL,Aki-Sener E,Yalcin I

    更新日期:2004-04-30 00:00:00

  • Immunocytochemical localization of the Ca2+-ATPase polypeptide in human platelets.

    abstract::Specific polyclonal antibodies raised against purified human platelet Ca2+-ATPase were used with protein A-gold immunocytochemistry to localize this protein in human platelets. Immunolabeling specifically detected Ca2+-ATPase over the surface connected membrane system (SCS) in sections of paraformaldehyde-fixed, Lowic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Herbener GH,Dean WL

    更新日期:1988-06-16 00:00:00

  • ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle.

    abstract::In this study, we describe the identification and cloning of a novel member of the nuclear receptor superfamily. This orphan receptor, referred to as ROR gamma, belongs to the ROR/RZR subfamily. The open reading frame of ROR gamma encodes a protein of 560 amino acid residues with a predicted molecular mass of 63 kD. T...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hirose T,Smith RJ,Jetten AM

    更新日期:1994-12-30 00:00:00

  • Stoichiometry of ATP hydrolysis and chlorophyllide formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus.

    abstract::Dark-operative protochlorophyllide (Pchlide) oxidoreductase (DPOR) is a nitrogenase-like enzyme catalyzing a reduction of the C17 = C18 double bond of Pchlide to form chlorophyllide a (Chlide) in bacteriochlorophyll biosynthesis. DPOR consists of an ATP-dependent reductase component, L-protein (a BchL dimer), and a ca...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nomata J,Terauchi K,Fujita Y

    更新日期:2016-02-12 00:00:00

  • Software for precise tracking of cell proliferation.

    abstract::We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to ad...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kurokawa H,Noda H,Sugiyama M,Sakaue-Sawano A,Fukami K,Miyawaki A

    更新日期:2012-01-20 00:00:00

  • Expression of functional thrombin receptors in xenopus oocytes injected with human endothelial cell mRNA.

    abstract::Human endothelial cell thrombin receptors were functionally expressed in Xenopus laevis oocytes by injection of RNA extracted from human umbilical vein endothelial cells. Oocytes injected with endothelial cell RNA responded to thrombin with a Ca2(+)-dependent depolarizing current whose size depended on the amount of R...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pipili-Synetos E,Gershengorn MC,Jaffe EA

    更新日期:1990-09-28 00:00:00

  • On the respective roles of the two proteins encoded by the Bacillus sphaericus 1593M toxin genes expressed in Escherichia coli and Bacillus subtilis.

    abstract::The 3.6 kb HindIII DNA fragment of B. sphaericus 1593M chromosomal DNA bears two genes encoding two polypeptides of 41.9 kDa (protein "42") and 51.4 kDa (protein "51"). DNA fragments carrying only one of these two genes when expressed in E. coli yield products that are inactive towards Culex larvae. The larvicidal act...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: de la Torre F,Bennardo T,Sebo P,Szulmajster J

    更新日期:1989-11-15 00:00:00

  • LAMP-2 mediates oxidative stress-dependent cell death in Zn2+-treated lung epithelium cells.

    abstract::Zinc is an essential element for the biological system. However, excessive exogenous Zn2+ would disrupt cellular Zn2+ homeostasis and cause toxicity. In particular, Zinc salts or ZnO nanoparticles exposure could induce respiratory injury. Although previous studies have indicated that organelle damage (including mitoch...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Qin X,Zhang J,Wang B,Xu G,Zou Z

    更新日期:2017-06-17 00:00:00

  • Supramolecular surfactants: amphiphilic polymers designed to disrupt lipid membranes.

    abstract::Simple polyesters derived from poly(ethylene glycol)s and alpha, omega-dicarboxylic acids exhibit a broad range of activity in disrupting phospholipid membranes. This activity has been analyzed by measuring the release of liposome-encapsulated 5(6)-carboxy-fluorescein (CF). Comparison with an analogous monomeric surfa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Regen SL,Jayasuriya N,Fabianowski W

    更新日期:1989-03-15 00:00:00

  • Structure and analysis of the bovine atrial natriuretic peptide precursor gene.

    abstract::The isolation and sequence analysis of the gene encoding the bovine atrial natriuretic peptide (ANP) precursor is described. The bovine-ANP coding sequences are located on three exons which are interrupted by two intervening sequences. Comparison of the bovine, human, rat and mouse ANP gene sequences reveals a common ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Vlasuk GP,Miller J,Bencen GH,Lewicki JA

    更新日期:1986-04-14 00:00:00

  • Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle.

    abstract::Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. To understand the possible cellular processes in which Nek2 participates during the cell cycle, we investigated the expression and subcellular localization of Nek2 in mitotic cells. The Nek2 protein...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ha Kim Y,Yeol Choi J,Jeong Y,Wolgemuth DJ,Rhee K

    更新日期:2002-01-18 00:00:00

  • Antibiotics-induced gut microbiota dysbiosis promotes tumor initiation via affecting APC-Th1 development in mice.

    abstract::Gut microbiota is critical for maintaining body immune homeostasis and thus affects tumor growth and therapeutic efficiency. Here, we investigated the link between microbiota and tumorgenesis in a mice model of subcutaneous melanoma cell transplantation, and explored the underlying mechanism. We found disruption of gu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu C,Ruan B,Jiang Y,Xue T,Wang Z,Lu H,Wei M,Wang S,Ye Z,Zhai D,Wang L,Lu Z

    更新日期:2017-06-24 00:00:00

  • Stimulation of sphingomyelin hydrolysis by cannabidiol in fibroblasts from a Niemann-Pick patient.

    abstract::The hydrolysis of sphingomyelin in cells derived from a Niemann-Pick patient was studied using both a labelled precursor and measurement of endogenous levels. In vitro exposure of the cells to cannabidiol resulted in a large decrease in both the relative and absolute amounts of this lipid; the drug had a smaller effec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Burstein S,Hunter SA,Renzulli L

    更新日期:1984-05-31 00:00:00

  • The role of MAP4K3 in lifespan regulation of Caenorhabditis elegans.

    abstract::The TOR pathway is a kinase signaling pathway that regulates cellular growth and proliferation in response to nutrients and growth factors. TOR signaling is also important in lifespan regulation - when this pathway is inhibited, either naturally, by genetic mutation, or by pharmacological means, lifespan is extended. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Khan MH,Hart MJ,Rea SL

    更新日期:2012-08-24 00:00:00

  • Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells.

    abstract::Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocyt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tang K,Yang J,Gao X,Wang C,Liu L,Kitani H,Atsumi T,Jing N

    更新日期:2002-04-26 00:00:00

  • Cloning and expression of a cDNA for human cytochrome P-450aldo as related to primary aldosteronism.

    abstract::A cDNA clone encoding human aldosterone synthase cytochrome P-450 (P-450aldo) has been isolated from a cDNA library derived from human adrenal tumor of a patient suffering from primary aldosteronism. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 3 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kawainoto T,Mitsuuchi Y,Ohnishi T,Ichikawa Y,Yokoyama Y,Sumimoto H,Toda K,Miyahara K,Kuribayashi I,Nakao K

    更新日期:1990-11-30 00:00:00

  • MT1-MMP prevents growth inhibition by three dimensional fibronectin matrix.

    abstract::The extracellular microenvironment plays a key role in regulation of cellular functions and growth control. We show here that membrane-type 1 matrix metalloproteinase (MT1-MMP) acts as a growth promoter in confluent culture. When MT1-MMP was silenced in HT1080 fibrosarcoma cells, cells created three dimensional (3D) f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takino T,Guo L,Domoto T,Sato H

    更新日期:2013-07-05 00:00:00

  • Glucose effect on the expression of 150 kDa oxygen-regulated protein in HeLa cells.

    abstract::Chaperones assist in the correct folding of newly synthesised proteins in the endoplasmic reticulum (ER) of cells, this being essential for the translocation of protein molecules to specific subcellular compartments, extracellular matrix or to biological fluids. The biosynthesis of some ER chaperones is regulated by g...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cechowska-Pasko M,Bańkowski E,Chene P

    更新日期:2005-11-25 00:00:00

  • Molecular tilting in dried elastoidin and its implications for the structures of other collagen fibrils.

    abstract::Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hukins DW,Woodhead-Galloway J,Knight DP

    更新日期:1976-12-20 00:00:00

  • Identification of genetic variation in the human serotonin 1D beta receptor gene.

    abstract::Disturbances of serotonergic pathways have been implicated in a wide variety of neuropsychiatric disorders such as depression, anxiety, migraine, and substance abuse. Genetic variation in genes coding for serotonin receptor proteins might well be involved in the genetic predisposition to these diseases and/or of pharm...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nöthen MM,Erdmann J,Shimron-Abarbanell D,Propping P

    更新日期:1994-12-15 00:00:00

  • S-Nitrosoglutathione induces functional DeltaF508-CFTR in airway epithelial cells.

    abstract::S-Nitrosoglutathione (GSNO) is an endogenous bronchodilator levels of which are reduced in the airways of cystic fibrosis (CF) patients. GSNO has recently been shown to increase maturation of CFTR in CF cell lines at physiological concentrations. The ability of S-nitrosoglutathione to direct the DeltaF508-CFTR to the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Andersson C,Gaston B,Roomans GM

    更新日期:2002-09-27 00:00:00

  • Specific internalization of estrogen and binding to nuclear matrix in isolated uterine cells.

    abstract::Fractionation of rat uterine cells incubated at 22 degrees C with 0.2 nM [3H]-estradiol-17 beta (E2 beta) was performed to analyze the subcellular distribution of internalized hormone. The postnuclear supernatant of homogenates was resolved in Percoll density gradients into six major fractions defined by enzyme marker...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pietras RJ,Szego CM

    更新日期:1984-08-30 00:00:00

  • Role of protein kinase C-alpha in activation of ecto-5'-nucleotidase in the preconditioned canine myocardium.

    abstract::We have reported that activation of protein kinase C (PKC) increases ecto-5'-nucleotidase activity, which may contribute to the infarct size-limiting effect of ischemic preconditioning. Since we have reported that Ca(2+)- and phospholipid-sensitive PKC is activated due to ischemic preconditioning, we further tested 1)...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kitakaze M,Funaya H,Minamino T,Node K,Sato H,Ueda Y,Okuyama Y,Kuzuya T,Hori M,Yoshida K

    更新日期:1997-10-09 00:00:00

  • Physiological role of the association complexes of alpha-crystallin and its substrates on the chaperone activity.

    abstract::Previous reports on the chaperone activity of alpha-crystallin to prevent protein denaturation and thermal aggregation have suggested that partially denatured proteins can bind alpha-crystallin in its central region. Likewise, beta- and gamma-crystallin can also be localized to the central cavity of alpha-crystallin p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee JS,Samejima T,Liao JH,Wu SH,Chiou SH

    更新日期:1998-03-17 00:00:00

  • Regulatory role of GM3 ganglioside in integrin function, as evidenced by its effect on function of alpha 5 beta 1-liposomes: a preliminary note.

    abstract::Mouse mammary carcinoma mutant cell line FUA169, characterized by high GM3 ganglioside content, was established from parent cell line FM3A/F28-7, which has high LacCer content but no GM3. Although both cell lines showed the same quantity and quality of integrin receptors, FUA169 showed much stronger adhesion to fibron...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zheng M,Tsuruoka T,Tsuji T,Hakomori S

    更新日期:1992-08-14 00:00:00

  • Calmodulin-dependent protein kinase IV during T-cell development.

    abstract::In a primary cell culture system of fetal rat brain, the calmodulin-dependent protein-kinase IV (CaMKIV) could be induced by the thyroid hormone T3 in a time- and concentration-dependent manner, provided the tissue was excised not later than day 15 of gestation (E15) (Krebs et al., J. Biol. Chem. 271, 11055, 1996). We...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Krebs J,Wilson A,Kisielow P

    更新日期:1997-12-18 00:00:00

  • Enhanced expression of Silencer of death domains (SODD/BAG-4) in pancreatic cancer.

    abstract::Pancreatic cancers are resistant to TNF-alpha-mediated apoptosis. Silencer of death domains (SODD) binds to TNF-alpha receptor TNFR-1, and prevents spontaneous self-association of death domains and inappropriate receptor signaling. In addition, overexpression of SODD suppresses TNF-alpha-induced cell death. In this re...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ozawa F,Friess H,Zimmermann A,Kleeff J,Büchler MW

    更新日期:2000-05-10 00:00:00

  • 1,25-Dihydroxyvitamin D3 enhancement of concanavalin-A induced bovine lymphocyte proliferation: requirement of monocytes.

    abstract::The effect of 1,25-(OH)2D3 on Concanavalin-A (ConA) mediated bovine lymphocyte proliferation was dependent on the magnitude of the proliferative response. Lymphocytes from cows with high inherent proliferative responses (134,904 +/- 15,488 cpm/well) were unaffected by 1,25-(OH)2D3; whereas, lymphocytes from cows with ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hustmyer FG,Nonnecke BJ,Beitz DC,Horst RL,Reinhardt TA

    更新日期:1988-04-29 00:00:00

  • Zinc-binding protein-89 (ZBP-89) cooperates with NF-κB to regulate expression of matrix metalloproteinases (MMPs) in response to inflammatory cytokines.

    abstract::Matrix metalloproteinases (MMPs) have both protective and pathological roles in inflammation, and transcriptional mechanisms are important in regulating physiological levels to maintain health. Zinc-binding protein-89 (ZBP-89) is a transcription factor with roles in regulating vital cellular processes, acting through ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Borghaei RC,Gorski G,Seutter S,Chun J,Khaselov N,Scianni S

    更新日期:2016-03-18 00:00:00

  • Determination of genes involved in the early process of molar root development initiation in rat by modified subtractive hybridization.

    abstract::Rat molars and incisors have different root patterning mechanisms: the former form a multirooted pattern while the latter form a single-rooted analogue. But the genetic signals that control root patterning are poorly understood. In this study, to identify the special molecular signals which may lead to the molar root ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xing X,Deng Z,Yang F,Watanabe S,Wen L,Jin Y

    更新日期:2007-11-30 00:00:00