Abstract:
:Isoforms of the recently cloned vesicular glutamate transporters (VGLUT1-3) selectively accumulate glutamic acid into synaptic vesicles in excitatory axon terminals and are viewed as reliable markers for glutamatergic neurons. Our present studies provided dual-label in situ hybridization evidence that virtually all (99.5%) GnRH neurons express VGLUT2 mRNA in the preoptic region of the adult male rat. Dual-label immunofluorescent experiments were carried out to examine the presence of VGLUT2 protein in GnRH axon terminals. Confocal laser microscopic analysis of the organum vasculosum of the lamina terminalis and the external zone of the median eminence, the major termination fields for GnRH-secreting axons, demonstrated the frequent occurrence of VGLUT2 immunoreactivity in GnRH axon terminals. Together these mRNA hybridization and immunocytochemical data indicate that GnRH neurons of the adult male rat possess marked glutamatergic characteristics. The physiological significance of endogenous glutamate in the regulation of gonadotropin secretion requires clarification.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Hrabovszky E,Turi GF,Kalló I,Liposits Zdoi
10.1210/en.2004-0589subject
Has Abstractpub_date
2004-09-01 00:00:00pages
4018-21issue
9eissn
0013-7227issn
1945-7170pii
en.2004-0589journal_volume
145pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::PTH-related protein (PTHrP) is the principle mediator of the syndrome of humoral hypercalcemia of malignancy and has potential paracrine actions on smooth muscle, epithelial cell growth, and placental calcium transport. The human PTHrP gene is complex: a combination of three promoters, one 5' alternative splicing even...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.4.8625910
更新日期:1996-04-01 00:00:00
abstract::The inhibitory effect of stress on reproductive function is potentially mediated by high concentrations of circulating glucocorticoids (GCs) acting via the GC receptor (GR). Gonadotropin-inhibitory hormone (GnIH) is a hypothalamic neuropeptide that inhibits gonadotropin secretion. GnIH may mediate stress-induced repro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2076
更新日期:2014-05-01 00:00:00
abstract::Progesterone, which is normally produced in the endoplasmic reticulum, was found to be rapidly degraded in the cytosolic fraction of the guinea pig adrenal cortex in vitro. Assuming this finding reflects what happens in vivo raises a question as to the source of progesterone for interacting with a nuclear progesterone...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1114
更新日期:1990-09-01 00:00:00
abstract::T4-binding globulin (TBG), the principal carrier of thyroid hormone in serum, is a glycoprotein containing 20% carbohydrate. The importance of the carbohydrate moiety has been previously studied by enzymatic deglycosylation, which showed that deglycosylated TBG retains its original immunological an T4-binding properti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1614
更新日期:1986-04-01 00:00:00
abstract::The adipokine resistin is an insulin-antagonizing factor that also plays a regulatory role in inflammation, immunity, food intake, and gonadal function. Although adipose tissue is the primary source of resistin, it is also expressed in other tissues and organs, including the pituitary. However, there is no information...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0116
更新日期:2009-10-01 00:00:00
abstract::Estrogen-based hormone therapy (HT) in postmenopausal women may reduce the risk of Alzheimer's disease (AD), although HT remains controversial. One key concern with HT is the potential of adverse outcomes such as breast and uterine cancer. A promising strategy to maximize HT benefits and minimize HT risks is the use o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1346
更新日期:2008-05-01 00:00:00
abstract::Specific receptors for tetradecapeptide somatostatin (S-14) in rat adrenal cortical membranes were quantitated by direct binding studies using [125I-Tyr11]S-14. Competitive inhibition of this radioligand by S-14 showed that these receptors constitute a single class of high affinity binding sites [dissociation constant...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-5-1717
更新日期:1985-05-01 00:00:00
abstract::Primary astroglial cells produce insulin-like growth factor (IGF)-binding proteins (IGFBPs), which modulate the biological activity of IGFs on the developing astroglia. Alterations in the synthesis of astroglial IGFBPs by hormones and growth factors may, therefore, influence the paracrine regulatory actions of IGFs. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.7691580
更新日期:1993-10-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP)-I has been reported to inhibit gastric acid secretion through stimulation of gastric somatostatin-14 (S-14) release. To establish whether some of the effects of CGRP-I on intestinal function might also be mediated through somatostatin, fetal rat intestinal cultures were treated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.7902270
更新日期:1993-12-01 00:00:00
abstract::Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains cha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0341
更新日期:2010-09-01 00:00:00
abstract::The experimental induction of puberty by GnRH administration to prepubertal lambs increases serum bioactive FSH (B-FSH) as measured in the rat Sertoli cell aromatase induction bioassay. Serum immunoreactive FSH (I-FSH) levels are unchanged. The increase in serum B-FSH is associated with an increase in the proportion o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.4.8462450
更新日期:1993-04-01 00:00:00
abstract::B-cell lymphoma 2 (BCL2) family kin (BFK) is a recently identified novel protein that is similar to proteins of the BCL2 family. In the present study, we discovered that the mouse Bfk transcript is expressed at the highest level in the epididymis. Two transcripts of 0.9 and 2.6 kb in size were identified, with alterna...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0018
更新日期:2007-07-01 00:00:00
abstract::To identify factors that regulate the tempo of growth and puberty, the present study examined how the environment influenced the timing of menarche and first ovulation in rhesus monkeys and how these events were related to differential rates of growth. Spring-born females were raised from 12 months of age under natura...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2653
更新日期:1988-12-01 00:00:00
abstract::Immunoreactive beta-endorphin (ir-beta EP) and immunoreactive N-acetyl-endorphin (ir-NacEP) have been demonstrated in the rat thyroid by specific RIA and characterized by reverse phase HPLC. In addition, pituitary and thyroid ir-beta EP and ir-NacEP levels have been determined after manipulation of the pituitary-thyro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-642
更新日期:1986-08-01 00:00:00
abstract::Epigenetic aberrations, including DNA methylation, histone modifications, and micro-RNA dysregulation, are now well established in the development and progression of ovarian cancer, and their gradual accumulation is associated with advancing disease stage and grade. Epigenetic aberrations are relatively stable, associ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2009-0404
更新日期:2009-09-01 00:00:00
abstract::In situ hybridization detected a transient, sex-specific transcription of somatostatin gene in the central part of the rat medial preoptic nucleus, which coincides with the sexually dimorphic nucleus of the preoptic area (SDN-POA), during, but not after, the establishment of sex difference. On postnatal d 1 (day of bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1214
更新日期:2007-03-01 00:00:00
abstract::We recently identified a 32 K mol wt insulin-like growth factor (IGF)-binding protein (BP) which is markedly increased in the serum of streptozotocin-diabetic rats and recognized by antiserum against the human amniotic fluid IGFBP (hIGFBP-1). In the present study we sought to confirm that this protein represents the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-789
更新日期:1990-08-01 00:00:00
abstract::The nature and role of the peptidergic innervation of the ovary were examined by determining the location and function of vasoactive intestinal peptide (VIP)-containing nerve fibers in the immature rat ovary. Immunohistofluorescence analysis of prepubertal ovaries using a specific VIP antibody revealed sparse delicate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1682
更新日期:1986-04-01 00:00:00
abstract::In the present study we have used a high affinity photoaffinity label (PAL) agonist (pGlu-His-Trp-Ser-125I-iodoTyr-D-Lys(para-N3-Benzoyl)-Leu-Arg-P roNHEt) and a PAL antagonist (NAcD2Nal-D4ClPhe-D3Pal-Ser-125I-iodo-NMeTyr-D-L ys(para-N3-Benzoyl)Leu-Lys(Isp) - Pro-D-Ala-NH2) to covalently label the GnRH receptor. Rat p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8393780
更新日期:1993-08-01 00:00:00
abstract::Endocrine-disrupting chemicals (EDCs) that interfere with the steroid axis can affect amelogenesis, leading to enamel hypomineralization similar to that of molar incisor hypomineralization, a recently described enamel disease. We investigated the sex steroid receptors that may mediate the effects of EDCs during rat am...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1342
更新日期:2016-11-01 00:00:00
abstract::Neurotensin (NT) is a peptide expressed in the brain and in the gastrointestinal tract. Brain NT inhibits food intake, but the effects of peripheral NT are less investigated. In this study, peripheral NT decreased food intake in both mice and rats, which was abolished by a NT antagonist. Using c-Fos immunohistochemist...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1329
更新日期:2016-09-01 00:00:00
abstract::Characterization of glycoconjugates synthesized by polarized immature rabbit uterine epithelial (UE) cells in vitro, their vectorial patterns of secretion, and regulation by ovarian steroid hormones are reported. Large (mol wt, greater than 230 kDa) sialomucoglycoproteins and hyaluronate were primarily (86-96%) secret...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1727700
更新日期:1992-01-01 00:00:00
abstract::Atrial natriuretic peptide (ANP), via its guanylyl cyclase (GC)-A receptor, plays a key role in the regulation of arterial blood pressure (ABP) and volume. Endothelial-restricted deletion of GC-A in mice [endothelial cell (EC) GC-A knockout (KO)] resulted in hypervolemic hypertension, demonstrating that the endotheliu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1360
更新日期:2009-05-01 00:00:00
abstract::Activin A is a dimeric protein hormone that regulates numerous cellular functions. A clear physiological role for this molecule in pregnancy is suggested by previous studies in the human, wherein activin A rises dramatically as women approach parturition. To determine whether the rodent is a suitable animal model for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5231
更新日期:1997-07-01 00:00:00
abstract::Gonadotropin (GTH)-inhibitory hormone (GnIH) is a novel hypothalamic neuropeptide that inhibits GTH secretion in mammals and birds by acting on gonadotropes and GnRH neurons within the hypothalamic-pituitary-gonadal axis. GnIH and its orthologs that have an LPXRFamide (X = L or Q) motif at the C terminus (LPXRFamide p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-2046
更新日期:2012-05-01 00:00:00
abstract::The proglucagon gene (glu) encodes the incretin hormone glucagon-like peptide-1 (GLP-1), produced in the intestinal endocrine L cells. We found previously that the bipartite transcription factor beta-catenin/T cell factor (cat/TCF), the major effector of the canonical Wnt signaling pathway, activates intestinal glu ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1142
更新日期:2008-05-01 00:00:00
abstract::Kisspeptin (encoded by the Kiss1 gene) is an important regulator of reproduction. In rodents, Kiss1 is expressed in two hypothalamic regions, the arcuate nucleus and anteroventral periventricular/ periventricular continuum, where it is regulated by sex steroids. However, the distribution, regulation, and functional si...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1498
更新日期:2011-05-01 00:00:00
abstract::Japanese quail, a species with a precocial pattern of development, were used as a model for studying the ontogeny of thyroid responses to TSH. Thiourea was administered to embryos early in incubation, and the effects were assessed by measuring thyroid 32P uptake, serum thyroid hormones, thyroid weights, and body weigh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1238
更新日期:1984-04-01 00:00:00
abstract::Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1028
更新日期:1990-09-01 00:00:00
abstract::We have previously established the value of 2-dimensional electrophoretic mRNA activity profiles for investigating the hepatic genomic response to several metabolic perturbations, such as thyroid hormone or GH treatment, diabetes, high carbohydrate diet, starvation, and uremia. We now report the effects of adrenalecto...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2669
更新日期:1985-06-01 00:00:00