Regulation of Kiss1 expression by sex steroids in the amygdala of the rat and mouse.


:Kisspeptin (encoded by the Kiss1 gene) is an important regulator of reproduction. In rodents, Kiss1 is expressed in two hypothalamic regions, the arcuate nucleus and anteroventral periventricular/ periventricular continuum, where it is regulated by sex steroids. However, the distribution, regulation, and functional significance of neural kisspeptin outside of the hypothalamus have not been studied and are poorly understood. Here, we report the expression of Kiss1 in the amygdala, predominantly in the medial nucleus of the amygdala (MeA), a region implicated in social and emotional behaviors as well as various aspects of reproduction. In gonadally intact rats and mice, Kiss1-expressing neurons were identified in the MeA of both sexes, with higher Kiss1 expression levels in adult males than females in diestrus. In rats, Kiss1 expression in the MeA changed as a function of the estrous cycle, with highest levels at proestrus. Next, we tested whether Kiss1 in the MeA is regulated by the circulating sex steroid milieu. Kiss1 levels in the MeA were low in gonadectomized mice and rats of both sexes, and treatment with either testosterone or estradiol amplified Kiss1 expression in this region. Testosterone's inductive effect on Kiss1 expression in the MeA likely occurs via estrogen receptor-dependent pathways, not through the androgen receptor, because dihydrotestosterone (a nonaromatizable androgen) did not affect MeA Kiss1 levels. Thus, in rodents, Kiss1 is expressed and regulated by sex steroids in the MeA of both sexes and may play a role in modulating reproduction or brain functions that extend beyond reproduction.






Kim J,Semaan SJ,Clifton DK,Steiner RA,Dhamija S,Kauffman AS




Has Abstract


2011-05-01 00:00:00














  • Hormonal control of uterine growth: alterations in luminal epithelial deoxyribonucleic acid synthesis after intraluminal application of estrogen.

    abstract::Administration of a single injection of estradiol (E2) causes a maximum increase in DNA synthesis in the uterine luminal epithelium approximately 24 h later. We previously reported that animals receiving a second injection of E2 15-18 h after the first show an apparent decrease in DNA synthesis measured in this cell t...


    pub_type: 杂志文章


    authors: Kirkland JL,Mukku VR,Hardy M,Stancel GM

    更新日期:1984-03-01 00:00:00

  • The role of gonadotropin-releasing hormone in murine preimplantation embryonic development.

    abstract::Previous studies have established the presence of an extrahypothalamic GnRH in a variety of tissues. GnRH receptor is known to be present in the placenta, which produces and secretes the decapeptide from the very early stages of placentation. We hypothesized that GnRH may play a role in the preimplantation development...


    pub_type: 杂志文章


    authors: Raga F,Casañ EM,Kruessel J,Wen Y,Bonilla-Musoles F,Polan ML

    更新日期:1999-08-01 00:00:00

  • Modulation of endogenous opioid influence on luteinizing hormone secretion by progesterone and estrogen.

    abstract::Studies were undertaken using the opiate receptor antagonist naloxone (NAL) to evaluate the relative influence of endogenous opioid peptides (EOP) on LH secretion in cycling and ovariectomized, steroid-treated adult rats. Intact animals received NAL (2 mg/kg, sc) or saline (control vehicle) at 0800 and 1400 h on estru...


    pub_type: 杂志文章


    authors: Gabriel SM,Simpkins JW,Kalra SP

    更新日期:1983-11-01 00:00:00

  • The Effects of 20-kDa Human Placental GH in Male and Female GH-deficient Mice: An Improved Human GH?

    abstract::A rare 20K isoform of GH-V (here abbreviated as GHv) was discovered in 1998. To date, only 1 research article has characterized this isoform in vivo, observing that GHv treatment in male high-fat fed rats had several GH-like activities, but unlike GH lacked diabetogenic and lactogenic activities and failed to increase...


    pub_type: 杂志文章


    authors: List EO,Berryman DE,Basu R,Buchman M,Funk K,Kulkarni P,Duran-Ortiz S,Qian Y,Jensen EA,Young JA,Yildirim G,Yakar S,Kopchick JJ

    更新日期:2020-08-01 00:00:00

  • The adipose obese gene product, leptin: evidence of a direct inhibitory role in ovarian function.

    abstract::Leptin, a recently-discovered hormonal product of the obese gene, circulates in the blood at levels paralleling those of fat reserves and regulates satiety and improves reproductive performance if injected into mice lacking circulating leptin. Therefore, we tested the hypothesis that leptin signals metabolic informati...


    pub_type: 杂志文章


    authors: Spicer LJ,Francisco CC

    更新日期:1997-08-01 00:00:00

  • Prostaglandins modulate hormonal effects on rabbit myometrial alpha 1-adrenergic responses.

    abstract::Estrogen increases the alpha 1-adrenergic contractile sensitivity of the rabbit uterus. Since estrogen treatment increases prostaglandin (PG) production by the perfused rabbit uterus, and PGs contribute to the alpha 1-adrenergic contractile response, we postulated that estrogen's effects on PG production or response m...


    pub_type: 杂志文章


    authors: Hurd WW,Riemer RK,Goldfien A,Roberts JM

    更新日期:1991-09-01 00:00:00

  • NADPH-cytochrome P-450 reductase from untreated rat testicular and liver microsomes: isolation and comparison.

    abstract::NADPH-cytochrome P-450 reductase (EC, the enzyme involved in progesterone 17-hydroxylation, was purified to apparent homogeneity by detergent solubilization of the microsomal fractions of liver and testis from untreated rats. The enzymes from these two tissues were then compared with regard to several paramet...


    pub_type: 杂志文章


    authors: Hales DB,Betz G

    更新日期:1986-08-01 00:00:00

  • An autocrine progesterone positive feedback loop mediates oxytocin upregulation in bovine granulosa cells during luteinization.

    abstract::During luteinization of bovine granulosa cells in vitro in the presence of insulin, or insulin plus forskolin, there is a massive upregulation not only of progesterone production, but also of the gene for the peptide hormone oxytocin, with secretion of the peptide into the medium. By using the progesterone receptor an...


    pub_type: 杂志文章


    authors: Lioutas C,Einspanier A,Kascheike B,Walther N,Ivell R

    更新日期:1997-11-01 00:00:00

  • Further evidence for a central stimulatory action of catecholamines on adrenocorticotropin release in the rat.

    abstract::Catecholamines may stimulate ACTH secretion during stress. To investigate the nature and site of such an action, plasma ACTH was measured in four groups of unanesthetized adult female rats with an indwelling carotid cannula. Sequential 300-microliter blood samples were taken 60 min, 30 min, and immediately before an i...


    pub_type: 杂志文章


    authors: Szafarczyk A,Malaval F,Laurent A,Gibaud R,Assenmacher I

    更新日期:1987-09-01 00:00:00

  • Selective deletion of leptin receptors in gonadotropes reveals activin and GnRH-binding sites as leptin targets in support of fertility.

    abstract::The adipokine, leptin (LEP), is a hormonal gateway, signaling energy stores to appetite-regulatory neurons, permitting reproduction when stores are sufficient. Dual-labeling for LEP receptors (LEPRs) and gonadotropins or GH revealed a 2-fold increase in LEPR during proestrus, some of which was seen in LH gonadotropes....


    pub_type: 杂志文章


    authors: Akhter N,CarlLee T,Syed MM,Odle AK,Cozart MA,Haney AC,Allensworth-James ML,Beneš H,Childs GV

    更新日期:2014-10-01 00:00:00

  • Physiological role of growth hormone (GH)-releasing factor and somatostatin in the dynamics of GH secretion in adult male rat.

    abstract::To investigate the physiological role of GRF and somatostatin (SRIF) in GH secretion in adult male rats, we prepared in vitro models using the perifusion system of cultured rat anterior pituitary cells exposed to various combinations of human GRF-(1-44)NH2 (hGRF) and SRIF. We studied the following three models on GRF ...


    pub_type: 杂志文章


    authors: Sato M,Takahara J,Fujioka Y,Niimi M,Irino S

    更新日期:1988-10-01 00:00:00

  • Age-related changes in pulsatile luteinizing hormone release precede the transition to estrous acyclicity and depend upon estrous cycle history.

    abstract::Aging of the female reproductive system in rats is marked by discrete stages in the disappearance of regular estrous cyclicity. We determined at which point in the transition to acyclicity changes in the LH pulse generator could be detected by comparing pulsatile LH release in ovariectomized young rats to three groups...


    pub_type: 杂志文章


    authors: Scarbrough K,Wise PM

    更新日期:1990-02-01 00:00:00

  • Ontogeny of hypothalamic luteinizing hormone-releasing hormone (GnRH) and pituitary GnRH receptors in fetal and neonatal rats.

    abstract::Although it is known that LH secretion starts at 17 days of gestation in the fetal rat and that this first LH release is most likely driven by hypothalamic GnRH, an earlier role for GnRH during fetal life has been postulated with the observation that presence of GnRH is important before day 13 of gestation for the dif...


    pub_type: 杂志文章


    authors: Aubert ML,Begeot M,Winiger BP,Morel G,Sizonenko PC,Dubois PM

    更新日期:1985-04-01 00:00:00

  • Coculture with primary visceral rat adipocytes from control but not streptozotocin-induced diabetic animals increases glucose uptake in rat skeletal muscle cells: role of adiponectin.

    abstract::We developed a coculture system comprising primary rat adipocytes and L6 rat skeletal muscle cells to allow investigation of the effects of physiologically relevant mixtures of adipokines. We observed that coculture, or adipocyte-conditioned media, increased glucose uptake in muscle cells. An adipokine that could pote...


    pub_type: 杂志文章


    authors: Vu V,Kim W,Fang X,Liu YT,Xu A,Sweeney G

    更新日期:2007-09-01 00:00:00

  • Cyclic nucleotide-dependent protein kinase modulators in thyroid tissue.

    abstract::A heat-stable protein which inhibits cAMP dependent protein kinase was prepared from the bovine thyroid 100,000 X g supernatant. This protein inhibited the cAMP-dependent protein kinase both from bovine thyroid cytosol and bovine thyroid plasma membranes. The inhibitory effect was noncompetitive with histone as substr...


    pub_type: 杂志文章


    authors: Hashizume K,DeGroot LJ

    更新日期:1979-07-01 00:00:00

  • Insulin mediators are the signal transduction system responsible for insulin's actions on human placental steroidogenesis.

    abstract::To test the hypothesis that insulin mediators serve as the signal transduction system for insulin's steroidogenic actions in human placental cytotrophoblasts, we examined the effects of two inositolglycan insulin mediators, the insulin pH 2.0 chiro-inositol mediator (IM-pH 2.0) and the insulin pH 1.3 myo-inositol medi...


    pub_type: 杂志文章


    authors: Nestler JE,Romero G,Huang LC,Zhang CG,Larner J

    更新日期:1991-12-01 00:00:00

  • Short-term in vivo inhibition of insulin receptor substrate-1 expression leads to insulin resistance, hyperinsulinemia, and increased adiposity.

    abstract::Insulin receptor substrate-1 (IRS-1) has an important role as an early intermediary between the insulin and IGF receptors and downstream molecules that participate in insulin and IGF-I signal transduction. Here we employed an antisense oligonucleotide (IRS-1AS) to inhibit whole-body expression of IRS-1 in vivo and eva...


    pub_type: 杂志文章


    authors: Araújo EP,De Souza CT,Gasparetti AL,Ueno M,Boschero AC,Saad MJ,Velloso LA

    更新日期:2005-03-01 00:00:00

  • Prolactin messenger ribonucleic acid levels in the normal and hypogonadal mouse pituitary gland.

    abstract::The effects of estrogen on the synthesis of pituitary PRL mRNA and PRL and the release of PRL into plasma were investigated in male and female normal and hypogonadal (hpg) mice. Because the hpg mouse is totally deficient in hypothalamic GnRH, it provides an excellent model for investigating the effects of estrogen and...


    pub_type: 杂志文章


    authors: Stanley HF,Curtis A,Sheward WJ,Roberts JL,Fink G

    更新日期:1986-12-01 00:00:00

  • In vitro evidence that an 11-kilodalton N-terminal fragment of proopiomelanocortin is a growth factor specifically stimulating the development of lactotrophs in rat pituitary during postnatal life.

    abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...


    pub_type: 杂志文章


    authors: Tilemans D,Andries M,Proost P,Devreese B,Van Beeumen J,Denef C

    更新日期:1994-07-01 00:00:00

  • Identification of binding proteins for nuclear localization signals of the glucocorticoid and thyroid hormone receptors.

    abstract::Nuclear entry of proteins the size of the glucocorticoid and thyroid hormone receptors appears to be mediated by an interaction of nuclear localization signals (NLSs) within the proteins and specific NLS-binding proteins. NLSs have been identified in the hinge region of both receptors. We have identified the cellular ...


    pub_type: 杂志文章


    authors: LaCasse EC,Lochnan HA,Walker P,Lefebvre YA

    更新日期:1993-03-01 00:00:00

  • Physiologically significant effect of neuropeptide Y to suppress growth hormone release by stimulating somatostatin discharge.

    abstract::Neuropeptide Y (NPY) is a peptide found in a variety of hypothalamic loci which is frequently colocalized with catecholamines. It is also secreted into hypophyseal portal vessels. The injection of NPY into the third ventricle (3V) lowered plasma GH levels in conscious, freely moving male rats. To determine the physiol...


    pub_type: 杂志文章


    authors: Rettori V,Milenkovic L,Aguila MC,McCann SM

    更新日期:1990-05-01 00:00:00

  • Rapid changes in anterior pituitary cell phenotypes in male and female mice after acute cold stress.

    abstract::The anterior pituitary (AP) is made of five different cell types. The relative abundance and phenotype of AP cells may change in different physiological situations as an expression of pituitary plasticity. Here, we analyze in detail the phenotype of mouse corticotropes and the effects of acute cold stress on AP cell p...


    pub_type: 杂志文章


    authors: Senovilla L,Núñez L,Villalobos C,García-Sancho J

    更新日期:2008-05-01 00:00:00

  • Thyroid hormone, retinoic acid, and testosterone suppress proliferation and induce markers of differentiation in cultured rat sertoli cells.

    abstract::This study uses a high purity cell culture system to extend previous observations of factors controlling the end of the Sertoli cell proliferative phase. Thyroid hormone, retinoic acid, and testosterone were assessed for their ability to halt the proliferative phase and regulate the expression of markers associated wi...


    pub_type: 杂志文章


    authors: Buzzard JJ,Wreford NG,Morrison JR

    更新日期:2003-09-01 00:00:00

  • Leuprolide, a gonadotropin-releasing hormone agonist, reestablishes spermatogenesis after 2,5-hexanedione-induced irreversible testicular injury in the rat, resulting in normalized stem cell factor expression.

    abstract::2,5-Hexanedione (2,5-HD) exposure in the rat produces irreversible testicular atrophy, a model of human male infertility that can be used for mechanistic and therapeutic studies. Following testicular injury by 2,5-HD, stem cell factor (SCF), a Sertoli cell-derived growth factor that binds the c-kit receptor on spermat...


    pub_type: 杂志文章


    authors: Blanchard KT,Lee J,Boekelheide K

    更新日期:1998-01-01 00:00:00

  • Up-regulation of advanced glycated products receptors in the brain of diabetic rats is prevented by antioxidant treatment.

    abstract::Diabetics have at least twice the risk of stroke and may show performance deficit in a wide range of cognitive domains. The mechanisms underlying this gradually developing end-organ damage may involve both vascular changes and direct damage to neuronal cells as a result of overproduction of superoxide by the respirato...


    pub_type: 杂志文章


    authors: Aragno M,Mastrocola R,Medana C,Restivo F,Catalano MG,Pons N,Danni O,Boccuzzi G

    更新日期:2005-12-01 00:00:00

  • Adrenocorticotropin response and nicotine-induced norepinephrine secretion in the rat paraventricular nucleus are mediated through brainstem receptors.

    abstract::Nicotine is a potent stimulus for the secretion of ACTH, and norepinephrinergic neurons originating in the brainstem are involved. Prior reports using in vivo microdialysis in alert rats have shown that nicotine, administered i.p. or into the fourth ventricle, stimulated the release of norepinephrine (NE) into the hyp...


    pub_type: 杂志文章


    authors: Fu Y,Matta SG,Valentine JD,Sharp BM

    更新日期:1997-05-01 00:00:00

  • Alpha 2-macroglobulin and tissue inhibitor of metalloproteinases: collagenase inhibitors in human preovulatory ovaries.

    abstract::Extensive remodeling of the follicular extracellular matrix occurs during the process of ovulation. This remodeling involves the breakdown of collagen, which is regulated, in part, by the action of the metalloproteinase collagenase and its associated inhibitors. In the present study, follicular metalloproteinase inhib...


    pub_type: 杂志文章


    authors: Curry TE Jr,Mann JS,Estes RS,Jones PB

    更新日期:1990-07-01 00:00:00

  • Impaired leptin expression and abnormal response to fasting in corticotropin-releasing hormone-deficient mice.

    abstract::Leptin has been postulated to comprise part of an adipostat, whereby during states of excessive energy storage, elevated levels of the hormone prevent further weight gain by inhibiting appetite. A physiological role for leptin in this regard remains unclear because the presence of excessive food, and therefore the nee...


    pub_type: 杂志文章


    authors: Jeong KH,Sakihara S,Widmaier EP,Majzoub JA

    更新日期:2004-07-01 00:00:00

  • Insulin-like growth factor II is a potent inhibitor of the aromatase activity of human placental cytotrophoblasts.

    abstract::The placenta is the primary source of estrogens and progesterone during pregnancy. Because pregnant diabetic women are reported to have lower serum estrogen and higher progesterone levels than nondiabetic pregnant women, and placental insulin-like growth factor II (IGF-II) production may be elevated during diabetic pr...


    pub_type: 杂志文章


    authors: Nestler JE

    更新日期:1990-11-01 00:00:00

  • Effects of human growth hormone on insulin-stimulated glucose metabolism in 3T3-F442A adipocytes.

    abstract::GH is believed to play a role in promoting insulin resistance in patients with diabetes and with GH excess. The means by which GH produces insulin resistance may be through direct suppression of glucose metabolism in target cells (insulin-independent) or by interfering with the ability of insulin to stimulate glucose ...


    pub_type: 杂志文章


    authors: Foster CM,Hale PM,Jing HW,Schwartz J

    更新日期:1988-08-01 00:00:00