Abstract:
:Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains challenging. Here, we describe the use of transgenic mice for cAMP imaging in living pituitary slices and primary pituitary cells. This technique can be widely used to assess the contribution of various pituitary receptors, including individual G(i) protein-coupled somatostatin receptors, to the regulation of cAMP levels under physiologically relevant settings.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Jacobs S,Calebiro D,Nikolaev VO,Lohse MJ,Schulz Sdoi
10.1210/en.2010-0341subject
Has Abstractpub_date
2010-09-01 00:00:00pages
4560-5issue
9eissn
0013-7227issn
1945-7170pii
en.2010-0341journal_volume
151pub_type
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