Abstract:
:PTH-related protein (PTHrP) is the principle mediator of the syndrome of humoral hypercalcemia of malignancy and has potential paracrine actions on smooth muscle, epithelial cell growth, and placental calcium transport. The human PTHrP gene is complex: a combination of three promoters, one 5' alternative splicing event and alternative 3' splicing, which produces three PTHrP isoforms (139, 141, or 173 amino acids), results in multiple PTHrP messenger RNA (mRNA) species. We employed the RT-PCR technique to identify promoter usage and splicing patterns in a range of human cell lines. Cell line-specific utilization of the promoters and the 3' alternative splicing pathways was detected among bone, breast, kidney, and lung cell lines, although each cell line could potentially produce the three PTHrP isoforms. We also determined whether some of the known regulators of PTHrP differentially modulate promoter usage or splicing patterns. Dexamethasone decreased the abundance of each of the alternative mRNA species. In contrast, epidermal growth factor and transforming growth factor-beta treatment increased the abundance of each PTHrP mRNA species, with particularly marked effects on promoter 1- and promoter 2-initiated transcripts, especially those containing exon VII or VIII. Epidermal growth factor treatment was found to alter PTHrP splicing patterns in a manner consistent with increased transcription from promoters 1 and 2 and stabilization of exon VII- and IX-containing transcripts.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Southby J,Murphy LM,Martin TJ,Gillespie MTdoi
10.1210/endo.137.4.8625910subject
Has Abstractpub_date
1996-04-01 00:00:00pages
1349-57issue
4eissn
0013-7227issn
1945-7170journal_volume
137pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::To determine the effect of aromatase inhibitors on peripheral aromatization, male rhesus monkeys were infused with [7-3H]androstenedione and [4-14C]estrone before and during treatment with 4-hdyroxy-androstene-3,17-dione (50 mg/kg each; four monkeys) or 4-acetoxy-androstene-3,17-dione (700 mg each; two monkeys). In al...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-1-19
更新日期:1980-01-01 00:00:00
abstract::Centrally administered histamine (HA) stimulates the secretion of adenohypophysial POMC-derived peptides, which subsequently cause release of corticosterone. The effect of HA on POMC-derived peptide release is indirect, and it is possible that hypothalamic neurons containing corticotropin-releasing hormone (CRH), argi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070360
更新日期:1994-09-01 00:00:00
abstract::The receptors for the two structurally related insulinotropic hormones Glucose-dependent Insulinotropic Polypeptide (GIP) and Glucagon-Like Peptide-1 (GLP-1) share approximately 40% sequence identity and demonstrate complete specificity for their endogenous ligands, while utilizing similar second messenger pathways. I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.6.9104
更新日期:1997-06-01 00:00:00
abstract::Prader-Willi syndrome (PWS) has a biphasic clinical phenotype with failure to thrive in the neonatal period followed by hyperphagia and severe obesity commencing in childhood among other endocrinological and neurobehavioral abnormalities. The syndrome results from loss of function of several clustered, paternally expr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0371
更新日期:2005-10-01 00:00:00
abstract::In the present study we have examined the effects of FSH, forskolin, and (Bu)2cAMP on messenger RNA (mRNA) levels for all known subunits of cAMP-dependent protein kinase in rat Sertoli cells, using newly developed complementary DNA (cDNA) probes. mRNAs for the three regulatory subunits [RI alpha, RII51, (RII beta), an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2658
更新日期:1988-06-01 00:00:00
abstract::In vivo studies have suggested that serotonin (5HT) influences anterior pituitary function at the hypothalamic level. The present in vitro study investigated the possibility that 5HT may act directly on the anterior pituitary. The high affinity uptake of [3H]5HT into adult rat anterior pituitary tissue was examined in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-3-754
更新日期:1982-03-01 00:00:00
abstract::The possibility of direct pituitary effects of sex steroids on gonadotropin gene expression and synthesis was studied in male rats. The animals were treated with a potent GnRH antagonist, Ac-D-pClPhe-D-pClPhe-D-Trp-Ser-Tyr-D-Arg-Leu-Arg-Pro-D-Ala-+ ++NH2CH3COOH (Org 30276; 0.5 mg/kg BW, sc, twice daily) for 10 days. G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-3204
更新日期:1990-06-01 00:00:00
abstract::Low basal GH secretion and reduced GH responsiveness to different GH secretagogues, including GHRF, have been reported in aged animals and humans. Parallel to the in vivo findings, an impaired GH responsiveness to GHRF is evident in somatotropes from old rats of either sex. We report here that in anterior pituitaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1649
更新日期:1987-11-01 00:00:00
abstract::The 3'5'-cyclic GMP (cGMP) pathway is known to influence ovarian functions, including steroidogenesis, ovulation, and granulosa cell proliferation. We show here that cGMP-phosphodiesterase (PDE) activity increased in a gonadotropin-dependent manner more than 3-fold in the cumulus-oocyte complex (COC) after 24 h in vit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0547
更新日期:2008-11-01 00:00:00
abstract::Nutritional programming, taking place in utero or early after birth, is closely linked with metabolic and appetite disorders in adulthood. Following the hypothesis that nutritional programming impacts hypothalamic neuronal organization, we report on discrepancies of multiple molecular and cellular early events that ta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0893
更新日期:2010-02-01 00:00:00
abstract::The effect of an i.v. administration of different doses (250, 500, and 1000 pmol) of ovine CRF (oCRF) on plasma glucose and insulin levels in lean and genetically obese fa/fa rats was investigated. In both phenotypes, i.v. CRF promoted a rapid (peak at 1 min) transient doubling of basal insulin levels without a concom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1312430
更新日期:1992-04-01 00:00:00
abstract::The injection of chlorpropamide into Brattleboro homozygous rats (di/di) has previously been shown to result in enhanced activation of renal medullary adenylate cyclase activity and increased renal medullary content of cAMP in response to 1-desamino-8-D-arginine vasopressin (dDAVP). In contrast, in vivo chlorpropamide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2025
更新日期:1982-12-01 00:00:00
abstract::Magnocellular hypothalamic neurons in the rat supraoptic nucleus (SON) normally produce either vasopressin (VP) or oxytocin (OT). Here we demonstrate that many magnocellular neurons in the SON of lactating rats synthesize both hormones at the same time. We show the colocalization of the messenger (m) RNA that encodes ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1814
更新日期:1991-10-01 00:00:00
abstract::The present studies were conducted to further evaluate inositol phosphate formation and metabolism in prostaglandin F2 alpha (PGF2 alpha)-stimulated bovine luteal cells. Corpora lutea were dispersed with collagenase, and luteal cells were prelabeled for 3 h with [3H]inositol. Inositol phosphates produced in response t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1519
更新日期:1991-03-01 00:00:00
abstract::Immune activation results in the activation of adrenal steroidogenesis and inhibition of gonadal steroidogenesis. Previous studies indicated that these effects were caused primarily by activation and suppression of the secretion of ACTH and LH, respectively. However, other evidence indicated a direct effect of the imm...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7780
更新日期:2000-11-01 00:00:00
abstract::Somatostatin-like immunoreactivity (SRIF-LI) has previously been demonstrated immunohistochemically in rat thyroid parafollicular cells. Studies were therefore performed to determine whether SRIF-LI was present in a transplantable medullary carcinoma of the thyroid (MCT) in the WAG/Rij strain of rat. SRIF-LI was found...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-5-1418
更新日期:1980-11-01 00:00:00
abstract::This investigation examined the effects of repeated injections of LH on ovarian function in the immature sheep approximately 12 weeks before the time of the first expected spontaneous ovulation. The frequency of endogenous LH pulses during the pretreatment period was approximately one pulse each 3 h. The first experim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1179
更新日期:1984-09-01 00:00:00
abstract::Corticotropin-releasing factor (CRF) overexpressing (OE) mice are a genetic model that exhibits features of chronic stress. We investigated whether the adaptive feeding response to a hypocaloric challenge induced by food deprivation is impaired under conditions of chronic CRF overproduction. Food intake response to a ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0723
更新日期:2009-01-01 00:00:00
abstract::The SMRT coregulator functions as a dual coactivator and corepressor for estrogen receptor-α (ERα) in a gene-specific manner, and in several studies its elevated expression correlates with poor outcome for breast cancer patients. A specific role of SMRT in breast cancer progression has not been elucidated, but SMRT kn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1002
更新日期:2014-09-01 00:00:00
abstract::Media from large vessel endothelial cells (pulmonary artery, aorta) contained intact connective tissue growth factor (CTGF) and a dominant 19-kDa band. N-terminal analysis of the 19-kDa band showed sequence corresponding to CTGF amino acid 181-190, suggesting that the 19-kDa band represented a proteolytic fragment of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6633
更新日期:1999-04-01 00:00:00
abstract::Apolipoprotein-E (apoE) has been shown by noncovalent binding and photoaffinity labeling with [125I]T4 to possess a single L-T4 binding site with a K5 of about 3 x 10(7) M-1 and a relative affinity for analogs of L-T4 = D-T4 = rT3 = triiodothyroacetic acid > L-T3. T4 binding was not affected by the flavonoid EMD 21388...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.8365370
更新日期:1993-09-01 00:00:00
abstract::We have recently identified gonadotropes as target cells for ATP action via ATP receptors of the P2U subtype. The present studies have used gonadotrope-derived alpha T3-1 cells to examine the possible signaling mechanisms subserving ATP action in gonadotropes. Addition of ATP produced a biphasic intracellular Ca2+ (Ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536620
更新日期:1996-01-01 00:00:00
abstract::Amphibian skin has functional characteristics of epithelium in the mammalian distal nephron and plays an important role in sodium and water metabolism in these animals. A peptide extracted from the skin of the Australian frog Crinia georgiana has been purified, has been determined to have the amino acid sequence of [A...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-1-359
更新日期:1988-01-01 00:00:00
abstract::Despite a wealth of evidence for CRH mediating stress-induced suppression of the hypothalamic GnRH pulse generator, and hence reproductive dysfunction, the site and mechanism of action remains elusive. The locus coeruleus (LC), a prominent noradrenergic brain stem nucleus, is innervated by CRH neurons, mediates severa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1053
更新日期:2005-01-01 00:00:00
abstract::Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis because a loss-of-function mutation in the SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1492
更新日期:2004-10-01 00:00:00
abstract::GH controls its own secretion through a mechanism involving short-loop feedback regulation of the synthesis and release of GH-releasing hormone (GHRH). GHRH neurons coexpress the peptide galanin, but the functional significance of this coexpression is unknown. In this study, we tested the hypotheses that 1) galanin ge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940350
更新日期:1996-12-01 00:00:00
abstract::The mechanisms by which bacterial endotoxin [lipopolysaccharide (LPS)] stimulates the hypothalamo-pituitary-adrenal axis (HPAA) have not been elucidated. The present study was designed to investigate the involvement of macrophages in plasma ACTH and corticosterone responses to LPS administration in rats using selectiv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-1-330
更新日期:1991-07-01 00:00:00
abstract::There are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium L-glutamate (MSG). In our study, newborn male pups were ip injected with MSG (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0640
更新日期:2006-03-01 00:00:00
abstract::Thyroid hormone produces a prompt, plasma membrane-mediated increase in several metabolic functions in the rat thymocyte. These effects of thyroid hormone require calcium and are inhibited by the beta-adrenergic antagonist alprenolol. In the present study, the interrelationship between thyroid hormone and adrenergic a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2240
更新日期:1988-05-01 00:00:00
abstract::This study was initiated to investigate the significance of uterine cell death and proliferation during the estrous cycle and early pregnancy and their correlation with sex steroids in hamsters where blastocyst implantation occurs in only progesterone-primed uteri. The results obtained in hamsters were also compared w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1555
更新日期:2006-05-01 00:00:00