Abstract:
BACKGROUND:A novel pancreatic carcinoma cell line, FAMPAC, was identified from investigation of poorly differentiated pancreatic adenocarcinoma cells found in a patient with a familial predisposition to pancreatic carcinoma. A gene responsible for familial pancreatic carcinoma has not been identified to date. METHODS:The FAMPAC cell line was characterized by its morphology, growth rate, tumorigenicity, and chromosomal analysis. Three known tumor suppressor genes, p16/CDKN2, BRCA2, and p53, all of which are important in the development of pancreatic carcinoma and frequently are involved in a variety of cancer syndromes, were analyzed. RESULTS:FAMPAC cells grew as an adhering monolayer in culture medium supplemented with 10% fetal bovine serum and formed tumors rapidly in nude mice. The doubling time ranged from 24 to 48 hours. Karyotype analysis demonstrated the complexity of chromosomal deletions and rearrangements. The cells were negative for ductal differentiation markers such as cytokeratin 7 and MUC1, indicating poor differentiation. Analysis of FAMPAC cells revealed overexpression of the mutated p53 gene (exon 5, codon 175: CGC --> CAC), the presence of a homozygous deletion in the p16 gene, and the presence of wild-type BRCA2 in the tested hot spots. CONCLUSIONS:To the authors' knowledge, FAMPAC is the first established human pancreatic carcinoma cell line associated with a familial background. FAMPAC is a tumorigenic cell line with a complex molecular pattern of mutations. These findings may be useful in understanding the mechanisms responsible for the development of sporadic or hereditary forms of pancreatic carcinoma.
journal_name
Cancerjournal_title
Cancerauthors
Eisold S,Ryschich E,Linnebacher M,Giese T,Nauheimer D,Wild A,Bartsch DK,Büchler MW,Schmidt Jdoi
10.1002/cncr.20193subject
Has Abstractpub_date
2004-05-01 00:00:00pages
1978-86issue
9eissn
0008-543Xissn
1097-0142journal_volume
100pub_type
杂志文章相关文献
CANCER文献大全abstract:BACKGROUND:Epidemiologic studies have recently related benzene hexachloride (BHC) to breast carcinoma risk. Experimental studies have also shown that beta-BHC is weakly estrogenic, hence supporting the alleged association. By directly comparing beta-BHC levels in breast adipose tissue from incident breast carcinoma cas...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0142(19990515)85:10<2212::aid-c
更新日期:1999-05-15 00:00:00
abstract:BACKGROUND:While the question of whether the trials of breast cancer screening have resulted in a reduction in breast cancer death has been the subject of much scrutiny, there has been less attention to the reduction in tumor size achieved by screening. METHODS:Size data for invasive breast tumors were assembled from ...
journal_title:Cancer
pub_type: 杂志文章,meta分析
doi:10.1002/cncr.11766
更新日期:2003-11-15 00:00:00
abstract:BACKGROUND:The authors administered a combination of docetaxel and topotecan with granulocyte-colony-stimulating factor (G-CSF) support in a Phase I study to define the maximum tolerated dose (MTD) of this regimen. METHODS:Patients with advanced-stage solid tumors were eligible for this trial if they had a Zubrod perf...
journal_title:Cancer
pub_type: 临床试验,杂志文章
doi:10.1002/cncr.20238
更新日期:2004-05-15 00:00:00
abstract::Seventeen cases of malignant melanoma of soft parts (clear cell sarcoma) are reported. The patients ranged from 9 to 70 years of age, but 13 were between 10 and 40 years of age. There were eight male patients and nine female patients. The most common tumor location (seven patients) was the foot, followed by the area a...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19900115)65:2<367::aid-cncr28206
更新日期:1990-01-15 00:00:00
abstract::Kaposi's sarcoma of the gingiva and skin developed in an HIV-negative renal transplant patient while he was receiving cyclosporine therapy. The Kaposi's sarcoma developed shortly after the patient had an acute infection with cytomegalovirus (CMV). Electron microscopy of the tumor's established cell line showed two typ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19900201)65:3<492::aid-cncr28206
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:There is global variation in the onset of hepatocellular carcinoma (HCC). The objective of the current study was to investigate the impact of country of birth on age at the time of HCC diagnosis in the United States. METHODS:Incident HCC cases diagnosed between 2000 and 2012 in the Surveillance, Epidemiolog...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.30246
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:This study was performed to determine the incidence of central venous device-related blood stream infection and thrombosis in patients treated with moderate dose continuous-infusion interleukin-2 (IL-2). METHODS:The records of 160 consecutive patients treated at the University of Wisconsin Hospital and Clin...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:2001-02-15 00:00:00
abstract:BACKGROUND:Cytologic distinction between follicle center lymphoma (FCL) and mantle cell lymphoma (MCL) is difficult with cytomorphology alone and requires immunophenotyping. The current study describes the distinction between follicle center and mantle cell lymphoma made with fine-needle aspiration (FNA) material. MET...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:1999-08-25 00:00:00
abstract:BACKGROUND:Efficient quality assurance and improvement measures are essential ingredients in a well organized cytology-based program for cervical carcinoma screening. Various pap smear review procedures, aiming for optimization of accuracy, are described throughout the literature. Evaluation and synthesis of those meth...
journal_title:Cancer
pub_type: 杂志文章,meta分析
doi:10.1002/cncr.10921
更新日期:2003-02-25 00:00:00
abstract::The 4F2 antigen is a cell-membrane glycoprotein which arises early in the G0-G1 phases of the cell cycle. This molecule is present in all established human cell lines and most malignant human cells. The authors used an indirect immunophosphatase method to study 50 squamous cell carcinomas of the larynx and ten lymph-n...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19901001)66:7<1493::aid-cncr2820
更新日期:1990-10-01 00:00:00
abstract::The current study was undertaken to reevaluate the histogenesis of adenomatoid tumor by an immunoperoxidase method of detecting factor VIII related antigen, a tissue specific marker for vascular endothelium. Three of eight adenomatoid tumors showed diffuse cytoplasmic staining of the cells lining the lumina. When thes...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19820901)50:5<932::aid-cncr28205
更新日期:1982-09-01 00:00:00
abstract:BACKGROUND:The objective of this study was to compare findings after a cytologic report of low-grade squamous intraepithelial lesion, cannot exclude high-grade squamous intraepithelial lesion (LSIL-H) with findings after a report of low-grade squamous intraepithelial lesion (LSIL). METHODS:A review of patient records ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.23984
更新日期:2008-12-25 00:00:00
abstract::An autopsy case of prostaglandin E-producing hepatocellular carcinoma with hypercalcemia is presented in this article. A 72-year-old man showed high serum calcium levels (14.2 to 17.3 mg/100 ml) and hypophosphatemia. The plasma level of immunoreactive parathyroid hormone was below the normal range. Administration of o...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19880501)61:9<1813::aid-cncr2820
更新日期:1988-05-01 00:00:00
abstract:LAY SUMMARY:During the coronavirus disease 2019 (COVID-19) pandemic, cancer screening decreased precipitously; home screening for colorectal cancer diminished less than that for colonoscopy and breast and cervical cancer screening. The authors have highlighted approaches for home cancer screening in addition to telemed...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.33274
更新日期:2021-02-15 00:00:00
abstract::Hepatoblastoma differs from the adult type of hepatoma in clinical and pathologic features. The ratio of fucosylation of serum alpha-fetoprotein (AFP) was determined in seven patients with hepatoblastoma and in 21 infants and children with otherwise elevated serum AFP, using the improved technique of lentil agglutinin...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19890601)63:11<2174::aid-cncr282
更新日期:1989-06-01 00:00:00
abstract::The diagnosis of brain metastasis from prostate carcinoma is rarely made antemortem, and has previously been reported only in patients with known extensive metastatic disease of long duration. A case of adenocarcinoma of the prostate presenting as a brain metastasis is described. Current concepts of metastatic spread ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19841015)54:8<1723::aid-cncr2820
更新日期:1984-10-15 00:00:00
abstract:BACKGROUND:Hospital services are expectantly reduced over the weekend, which may result in a delay in treatment or in obtainment of medical procedures. The authors investigated quality of care and clinical outcomes of newly diagnosed acute myeloid leukemia (AML) patients who were hospitalized on weekends versus weekday...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.25086
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Sedentary behaviors are associated with poor health outcomes in the general population, but their clinical impact on adult survivors of childhood acute lymphoblastic leukemia (ALL) has not been characterized to date. In the current study, we described the prevalence of sedentary behaviors in survivors of ALL...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.31162
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Idarubicin (IDR), an anthracycline that is a derivative of daunorubicin, was synthesized in an attempt to find new analogs of daunorubicin with an improved spectrum of activity and diminished acute or chronic toxicity. Because of the favorable pharmacokinetic profile of IDR (with the persistence of its activ...
journal_title:Cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/(sici)1097-0142(19980815)83:4<813::aid-cnc
更新日期:1998-08-15 00:00:00
abstract:BACKGROUND:Breast conservation is now established treatment for patients with small breast cancers. The authors reviewed a large series of patients with long term follow-up who underwent conservative treatment. Clinical and pathologic factors were analyzed to identify patients at an increased risk of relapse in the bre...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19951201)76:11<2260::aid-cncr282
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND:Prostatic cancer is an unusual malignancy because, commonly, it is slowly progressive and occurs at an age where the patient's life expectancy is short as a result of considerable competing mortality rates from other diseases. In addition, the place of radical surgery, i.e., radical prostatectomy is still no...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19930201)71:3+<992::aid-cncr2820
更新日期:1993-02-01 00:00:00
abstract::Blood vessel invasion and axillary lymph node involvement were examined in 175 breast cancer patients. The incidence of blood vessel invasion was 35%. The presence of blood vessel invasion was highly associated with early disease recurrence. The association of poor prognosis with blood vessel invasion was independent ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19820901)50:5<962::aid-cncr28205
更新日期:1982-09-01 00:00:00
abstract:BACKGROUND:In spite of many reports, it remains unclear whether the presence of tumor cells in circulating blood flow predicts a poor prognosis. METHODS:Competitive seminested reverse transcriptase-polymerase chain reaction (RT-PCR), a technique for the quantitative detection of tumor cells, was applied to detect the ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(20010901)92:5<1251::aid-cncr1445
更新日期:2001-09-01 00:00:00
abstract::The kinetics of initial arrest in organs, distribution, survival, and fate of 125I-iododeoxyuridine-labeled B16 melanoma tumor cells injected intravenously into normal, tumor-sensitized and immune manipulated syngeneic and allogeneic mice were investigated. Groups of animals were killed at intervals ranging from two m...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197707)40:1<46::aid-cncr28204001
更新日期:1977-07-01 00:00:00
abstract:BACKGROUND:The optimal treatment of the primary tumor in patients with brain metastases (BM) from newly diagnosed nonsmall cell lung cancer (NSCLC) remains unclear. The authors aimed to identify patient groups with synchronous BM for whom radical treatment of the primary site may be appropriate. METHODS:The medical re...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.25416
更新日期:2011-02-01 00:00:00
abstract::Acute nonlymphocytic leukemia (ANLL) developed in four patients 66 to 157 months following the diagnosis of nodular poorly differentiated lymphocytic lymphoma (PDL-N). The ANLL was signalled by increasing anemia and thrombocytopenia. A characteristic cytologic pattern of the leukemia was found. In each patient, a panm...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197710)40:4<1748::aid-cncr282040
更新日期:1977-10-01 00:00:00
abstract:BACKGROUND:Six human pancreatic carcinoma cell lines, designated as KMP-1 to KMP-6, were established and maintained in vitro for > 3 years. All were derived from pancreatic ductal adenocarcinomas. The six cell lines originated from either primary pancreatic tumors, metastatic liver tumors, or metastases to lymph nodes....
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0142(19990215)85:4<832::aid-cnc
更新日期:1999-02-15 00:00:00
abstract::The relationships between cigarette smoking, occupational exposures, dietary vitamin A and lung cancer is explored in a case-control study. The study is based on 1002 primary lung cancers among white men and 1119 white male controls between 35 and 79 years of age admitted to Roswell Park Memorial Institute from 1957 t...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19850815)56:4<910::aid-cncr28205
更新日期:1985-08-15 00:00:00
abstract::This is a statistical analysis of the use of mammography in the symptomatic patient. Eighty-eight percent of women older than 50 years who had a palpable cancer of the breast had a positive mammogram; only 57% of women younger than 51 years of age had a positive mammogram. When the cancer presented as nipple discharge...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19890401)63:7<1412::aid-cncr2820
更新日期:1989-04-01 00:00:00
abstract::Six patients with breast cancer developed pulmonary toxicity following mitomycin therapy. The presenting symptoms were shortness of breath and a dry cough. The radiological pictures varied from a normal chest x-ray to extensive bilateral pneumonitis. The histological findings consisted of diffuse alveolar damage progr...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19800115)45:2<236::aid-cncr28204
更新日期:1980-01-15 00:00:00