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A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions.

Abstract：

:We developed a method for site-selective CpG methylation of the budding yeast genome. The method recruits LexA-fused M.SssI DNA methyltransferase to LexA operator sequences integrated adjacent to the target site. Microarray analysis of methylated DNAs indicated that the tethered enzyme selectively methylates the region around the target site. Exploiting this method to methylate bait DNA in the one-hybrid system, we demonstrated methylation-dependent DNA binding of methyl-CpG binding proteins, MBD1 and Kaiso, in vivo. This methylation-dependent one-hybrid system would provide a versatile tool for the search and analysis of proteins that recognize methylated DNA to participate in epigenetic regulation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Feng SY,Ota K,Yamada Y,Sawabu N,Ito T

doi

10.1016/j.bbrc.2003.12.027

subject

Has Abstract

pub_date

2004-01-23 00:00:00

pages

922-5

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X03026226

journal_volume

313

pub_type

杂志文章

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