Abstract:
:A cytometrical image analyzing method for nuclear protein was established using WinROOF, a commercially available, inexpensive software, to determine the status of both estrogen and progesterone receptors. Immunohistochemical evaluation of estrogen receptors (ER) and progesterone receptors (PR) was performed with the anti-ER (clone 1D5) and the anti-PR (clone PgR636), respectively, combined with dextran polymer reagent EnVision+, all of which are approved in vitro diagnostics in Japan. The immunostained results were captured as digital images in Windows, and then analyzed in WinROOF with macroinstructions for analyzing each captured area either immunolabeled with chromogen or counterstained with hematoxylin. This image analysis method graded the immunostained nuclei of carcinoma cells based on staining intensities, and calculated the labeling index (LI) for both ER and PR. Furthermore, the LI correlated highly with the results from a histology score (HSCORE) when 20 breast carcinomas were quantified. Regarding ER, when 20% in the LI was considered as the cut-off point for positive, the positivity of ER in computer-assisted analysis was 75% (15 of 20 cases), and was completely concordant with that of HSCORE-based analysis. These results indicate that the cytometrical image analysis-based quantification could be appropriately applied to the objective determination of the immunohistochemical status of both ER and PR.
journal_name
Pathol Intjournal_title
Pathology internationalauthors
Hatanaka Y,Hashizume K,Nitta K,Kato T,Itoh I,Tani Ydoi
10.1046/j.1440-1827.2003.01547.xsubject
Has Abstractpub_date
2003-10-01 00:00:00pages
693-9issue
10eissn
1320-5463issn
1440-1827pii
1547journal_volume
53pub_type
杂志文章abstract::An autopsy case of malignant mesothelioma with asbestosis caused by asbestos exposure for 17 years is reported. Autopsy revealed that mesothelioma spread extensively in all serosal tissues including pleura, pericardium, diaphragm, peritoneum and tunica vaginalis testis. Histopathologically, most of the tumor showed an...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1994.tb02927.x
更新日期:1994-10-01 00:00:00
abstract::In pulmonary fibrosis, it is known that fibrotic changes develop in the intra-alveolar spaces and that intra-alveolar fibrosis can be classified into three patterns, namely intra-alveolar buds, mural incorporation and obliterative changes. In order to clarify the evolution of intra-alveolar fibrosis, immunohistochemic...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1995.tb03503.x
更新日期:1995-08-01 00:00:00
abstract::Teratoid Wilms' tumor is an unusual morphological entity characterized by a classic triphasic malignancy with predominantly heterologous tissue. The authors describe two cases of teratoid Wilms' tumor with an extrarenal site: one in a 13-year-old girl with vaginal spotting (patient 1) and another in a 1-day-old girl w...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2009.02468.x
更新日期:2010-01-01 00:00:00
abstract::Pulmonary tumor thrombotic microangiopathy (PTTM) is a rare clinicopathological entity causing severe pulmonary hypertension. Its histological features include widespread tumor emboli along with fibrocellular intimal proliferation and thrombus formation in the small arteries and arterioles of the lungs. The result is ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2005.01783.x
更新日期:2005-01-01 00:00:00
abstract::Diffuse pulmonary ossification (DPO) is a rare condition characterized by diffuse metaplastic bone formation in the lungs. Two patterns have been described: dendriform, with a coral-like network of bone spiculae along the alveolar septa; and nodular, with lobulated fragments in the alveolar spaces. Dendriform DPO is f...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12228
更新日期:2015-01-01 00:00:00
abstract::A unique case of duodenal stromal tumor in a 51-year-old man is reported. The tumor histologically showed spindle cell proliferation and numerous eosinophilic globules. Most globules were composed of tangled 45 nm thick fibrils, which were ultrastructurally identical to 'skeinoid fibers'. The presence of glycogen gran...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1997.tb04459.x
更新日期:1997-11-01 00:00:00
abstract::Differentiation of malignant pleural mesothelioma (MPM) from benign mesothelial proliferation remains problematic. Loss of nuclear staining of BRCA1-associated protein 1 (BAP1; detected using immunohistochemistry (IHC)) and homozygous deletion (HD) of p16 (detected using fluorescence in situ hybridization (FISH)) are ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12453
更新日期:2016-10-01 00:00:00
abstract::An 18-year-old man who suffered from panniculitis involving the entire left lower limb after exertional rhabdomyolysis is reported. A high fever (>39 degrees C) and leukocytosis (>20,000/microL) persisted for 1 week, and his general status deteriorated rapidly into pre-disseminated intravascular coagulation, complicat...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2000.01127.x
更新日期:2000-10-01 00:00:00
abstract::Malignant transformation of ovarian mature cystic teratomas is reported to occur in about 1% of cases. The most common malignant evolution is that of squamous cell carcinoma and one of the least common is that of small cell carcinoma. We report a case of distant metastasis from a small cell carcinoma arising in a matu...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1998.tb03846.x
更新日期:1998-10-01 00:00:00
abstract::We investigated the diagnostic and prognostic value of p53 expression and proliferative activity, as indicated by the Ki-67, in endoscopic biopsy specimens. Specimens were immunologically stained with p53 and MIB-1 (Ki-67), and the MIB-1/Ki-67 labeling index (LI) was calculated. Classification of adenomas was based on...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2003.01482.x
更新日期:2003-06-01 00:00:00
abstract::To determine the prognostic significance of the methods used to determine the presence of metastasis in second-tier lymph nodes of patients with gastric cancer, the authors studied lymph nodes surgically removed from 100 patients with gastric cancer (55 with early cancer, 45 with progressive). The results of HE staini...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2007.02187.x
更新日期:2008-01-01 00:00:00
abstract::A case of primary esophageal adenocarcinoma in a 64-year-old man is reported. An ulcerating tumor was located in the middle intrathoracic esophagus. Histopathological examination revealed a moderately differentiated adenocarcinoma, which had invaded down to the adventitia. The cancerous tubuli were lined by flattened ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.1999.00838.x
更新日期:1999-02-01 00:00:00
abstract::Pilomyxoid astrocytoma, first described by Tihan et al., was recently included as an established variant of pilocytic astrocytoma in the World Health Organization classification of CNS tumors. Histologically, it much resembles pilocytic astrocytoma, but monomorphic myxoid tumor of pilocytic cells with prominent angioc...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2008.02332.x
更新日期:2009-02-01 00:00:00
abstract::A case of multiple myeloma, who died of congestive heart failure of unknown cause, is presented. Microscopically, Anitschkow cells with caterpillar and owl-eyed nuclei were scattered in the interstitium of the myocardium. To know the pathological significance of their appearance, histological observations of normal, d...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.1999.00827.x
更新日期:1999-01-01 00:00:00
abstract::Microscopic colitis (MC), encompassing collagenous colitis and lymphocytic colitis, is featured by chronic diarrhea, normal-looking endoscopic findings and unique microscopic appearance. After reviewing biopsied nonspecific colitis, we propose the third type of MC: colitis nucleomigrans (CN). Histopathological criteri...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12996
更新日期:2020-10-01 00:00:00
abstract::We report a case of gastrointestinal stromal tumor (GIST) of the stomach mimicking extragastrointestinal origin. The tumor presented as a large isolated mass in the transverse mesocolon with a minor adhesion to the stomach. Microscopic examination revealed c-kit gene protein product (KIT)-positive tumor cells with epi...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2011.02719.x
更新日期:2011-11-01 00:00:00
abstract::A pulmonary Langerhans cell histiocytosis is presented in a 40 year-old woman two years after bilateral lung transplantation for emphysema without any signs of Langerhans cells proliferation in the explanted lungs. A microsatellite molecular analysis showed the proliferating cells were generated in a recipient cellula...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12597
更新日期:2017-12-01 00:00:00
abstract::A 79-year-old man presented with back pain. Chest CT scan showed elevated nodular lesions in the right parietal pleurae with pleural effusion. There were no intrapulmonary or mediastinal abnormalities. Under local anesthesia, right thoracoscopy and subsequent thoracoscopic pleural resection were performed using an ins...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2008.02220.x
更新日期:2008-04-01 00:00:00
abstract::An autopsy case of giant cell myocarditis (GCM) in a 74-year-old woman is presented. She suffered from hepatic dysfunction, skin eruption and disseminated intravascular coagulation due to the side-effects of a non-steroidal anti-inflammatory drug. After admission, heart failure progressed rapidly, and the patient died...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2001.01166.x
更新日期:2001-02-01 00:00:00
abstract::We report a case of ALK-positive renal cell carcinoma coincident with Hodgkin lymphoma. The patient was a 19 year-old-girl without sickle cell trait. The right renal tumor was discovered concomitantly with Hodgkin lymphoma (HL). After chemotherapy for HL, right nephrectomy was performed. Microscopically, the tumor sho...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12592
更新日期:2017-12-01 00:00:00
abstract::A case of low-grade endometrial stromal sarcoma with extensive epithelial-like element (ELE) is reported. This tumor was composed of classical endometrial stromal sarcoma (CESS) showing diffuse proliferation, and ELE occupying approximately 72% of the tumor mass. On immunohistochemistry, ELE was negative for sex-cord ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1320-5463.2003.01463.x
更新日期:2003-04-01 00:00:00
abstract::Phyllodes tumor is a very rare neoplasm which accounts for 2.5% of all fibroepithelial lesions of the breast. The mesenchymal component of a malignant phyllodes tumor frequently contains heterologous components. We report a case of malignant phyllodes tumor. The patient was a 40-year-old woman with a lump on the left ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2001.01195.x
更新日期:2001-04-01 00:00:00
abstract::The purpose of the present study was to clarify the difference of expression of two stem cell markers, nestin and c-kit, among various pancreatic epithelial tumors and evaluate their utility. Immunohistochemistry was done for 99 surgically resected pancreatic tumor specimens, including 20 ductal adenocarcinoma (DAC), ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2007.02143.x
更新日期:2007-09-01 00:00:00
abstract::We established a dedifferentiated liposarcoma cell line (NDDLS-1) that produces interleukin-6 (IL-6) and granulocyte-colony stimulating factor (G-CSF). The parental tumor showed high leukemoid reactions. The NDDLS-1 cell line was established from a pleural effusion associated with a lung metastasis. Pleomorphic tumor ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2011.02683.x
更新日期:2011-08-01 00:00:00
abstract::We describe a case of minute pancreatic ductal adenocarcinoma featuring stenosis of the main pancreatic duct (MPD) and associated with histological findings of periductal elastosis and fibroblast proliferation. A 53-year-old Japanese man with preoperative radiological evidence of MPD stricture and dilation of the dist...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2002.01383.x
更新日期:2002-09-01 00:00:00
abstract::The purpose of the present study was to determine whether the amount and the location of residual tumor are associated with outcome in surgically treated rectal cancer patients who receive preoperative chemoradiation therapy. Forty-three rectal cancer patients who underwent sphincter-saving operations after preoperati...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2009.02464.x
更新日期:2009-12-01 00:00:00
abstract::An unusual case of uterine clear cell tumor is reported in a 56-year-old woman. The neoplasm was identified as a peculiar low-grade leiomyosarcoma, composed mainly of watery, clear large cells with round, fairly regular nuclei. No appreciable pleomorphism or high mitotic activity was noted. There was no necrosis. The ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1998.tb03833.x
更新日期:1998-01-01 00:00:00
abstract::Ovarian non-gestational choriocarcinomas co-existing with adenocarcinoma are extremely rare and have been reported as epithelial ovarian carcinomas of a "non-germ cell origin" with "choriocarcinomatous differentiation". Although the cellular origin of non-gestational choriocarcinoma may be post-meiotic ovarian germ ce...
journal_title:Pathology international
pub_type:
doi:10.1111/pin.12445
更新日期:2016-09-01 00:00:00
abstract::The incidence of lymphoma has rapidly increased over the last 40 years in Japan, following a trend that is very similar to that of breast cancer. In particular, the relative frequency of follicular lymphoma (FL) has reached that in Western countries. Given its indolence, a "watch-and-wait" approach is often applied to...
journal_title:Pathology international
pub_type: 杂志文章,评审
doi:10.1111/pin.12733
更新日期:2018-12-01 00:00:00
abstract::The alpha subunit of a GTP-binding protein, Go, was investigated in pulmonary neuroendocrine neoplasms and fetal tissues of the lung by an immunohistochemical method. Positive immunostaining for the alpha subunit of Go (Go alpha) was found predominantly on the cell membrane and found occasionally in the cytoplasm. Typ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1996.tb03629.x
更新日期:1996-06-01 00:00:00