Cicerarin, a novel antifungal peptide from the green chickpea.

Abstract:

:A peptide designated cicerarin, with an N-terminal amino acid sequence VKSTGRADDDLAVKTKYLPP dissimilar from known proteins and peptides and a molecular mass of 8kDa, was isolated from seeds of the green chickpea Cicer arietinum cv green chickpea. Cicerarin was isolated with a procedure that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Cicerarin was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel in 10mM Tris-HCl buffer (pH 7.3). Cicerarin exerted antifungal activity against Botrytis cinerea, Mycosphaerella arachidicola, and Physalospora piricola. The antifungal activity was preserved after exposure to 100 degrees C for 15min.

journal_name

Peptides

journal_title

Peptides

authors

Chu KT,Liu KH,Ng TB

doi

10.1016/s0196-9781(03)00134-7

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

659-63

issue

5

eissn

0196-9781

issn

1873-5169

pii

S0196978103001347

journal_volume

24

pub_type

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