Abstract：
:Cyclin D1 plays an important role in the multi-step process of gastrointestinal tumorigenesis. We hypothesize that normal enterocytes over-expressing cyclin D1 will demonstrate a transformed phenotype. The nontumorigenic intestinal epithelial cell line, IEC-18, was transfected with the vector pMV7-CCND1, encoding cyclin D1. Three clones, with cyclin D1 levels similar to those seen in colon cancer cell lines, were further evaluated in comparison to the vector control cells. They proliferated faster and demonstrated anchorage-independent growth in soft agar, higher saturation density, and higher plating efficiency. When injected into nude mice, tumors were generated after 6-8 weeks. On the other hand these cells were more sensitive to induction of apoptosis. There was no change in the level of beta-catenin protein. In conclusion, cyclin D1 can act as an oncogene in vitro and in vivo, when produced in immortalized normal intestinal epithelial cells. This model may be useful for understanding the role and interrelationships of cyclin D1 in colorectal tumorigenesis.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Kazanov D,Shapira I,Pick M,Kolker O,Liberman E,Deutsch V,Strier L,Dvory-Sobol H,Kunik T,Arber Ndoi
10.1023/a:1024138605802subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1251-61issue
7eissn
0163-2116issn
1573-2568journal_volume
48pub_type
杂志文章abstract：BACKGROUND:MicroRNA is essential for the malignant progression of human gastric cancer (GC), which is a leading cause of cancer deaths. However, the mechanism is still not so clear. AIMS:In our present research, we investigated the effect of miR-9-5p in GC. METHODS:We detected miR-9-5p expression in human gastric epi...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05626-2
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:Hepatic encephalopathy is a syndrome whose physiopathology is poorly understood; therefore, current diagnostic tests are imperfect and modern therapy is nonspecific. Particularly, it has been suggested that inflammation plays an important role in the pathogenesis of portal hypertensive encephalopathy in the ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1625-y
更新日期：2011-08-01 00:00:00
abstract：:Achalasia is an esophageal motility disorder of unknown etiology. Several studies suggest possible herpes or measles virus etiology, but results are inconclusive. The aim of this study was to test whether herpesvirus (HV), measles (MV), or human papilloma virus (HPV) sequences could be detected in myotomy specimens fr...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1018805600276
更新日期：1997-02-01 00:00:00
abstract：:We evaluated the alveolar-arterial oxygen difference (deltaA-a) and the ratio between PaO2 and the fractional concentration of inspired oxygen (P/F) in acute pancreatitis. Eleven patients had mild uncomplicated disease, six showed acute abdominal fluid collections, six had acute abdominal collections and asymptomatic ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1019668728058
更新日期：2002-09-01 00:00:00
abstract：:Occult gastrointestinal bleeding occurs in 8-30% of marathon runners. We hypothesized that cimetidine would decrease bleeding by reducing acid-mediated injury and conducted a blinded, placebo-controlled prospective trial to determine the impact of cimetidine on gastrointestinal symptoms and bleeding during a marathon....
journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01296804
更新日期：1991-10-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection has an estimated prevalence of 3% around the world. Unfortunately, many persons with HCV infection are asymptomatic. The aim of this study was to determine the prevalence of hepatitis C Virus infection in asymptomatic persons. This study was carried out in a population-based cross-sec...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-005-2565-1
更新日期：2005-04-01 00:00:00
abstract：:Serum and urine concentrations of pancreatic polypeptide (PP) were measured during experimental acute hemorrhagic pancreatitis and small-bowel infarction in dogs. The mean serum PP concentration in animals with pancreatitis increased markedly after 1 hr and remained elevated, while the mean serum PP concentration in t...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01314764
更新日期：1981-11-01 00:00:00
abstract：INTRODUCTION:A significant proportion of patients with Crohn's disease (CD) lose response to antibodies directed against tumor necrosis factor α (TNF). Prior TNF-antagonist failure is associated with lower rates of response to subsequent TNF-antagonist therapy. In patients failing two anti-TNF agents, a choice exists b...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1896-3
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND:Data on alcohol-related HCC are limited. AIMS:Our aim was to describe the incidence, management, and prognosis of alcohol compared to Hepatitis C (HCV)-related HCC at a national level. METHODS:Incident cases of HCC were identified in French healthcare databases between 2009 and 2012 and analyzed retrospect...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05724-1
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Systemic sclerosis (SS) patients with severe esophageal affection have impaired peristalsis. However, motor function evaluated in vivo by manometry and fluoroscopy does not provide detailed information about the individual contraction cycles. AIMS:To apply, for the first time in gastrointestinal (GI) patien...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1777-9
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND AND AIM:Dietary fiber shortens gut transit time, but data on the effects of fiber components (including resistant starch, RS) on intestinal contractility are limited. We have examined RS effects in male Sprague-Dawley rats fed either a high-amylose maize starch (HAMS) or a wholemeal made from high-amylose wh...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-015-3537-8
更新日期：2015-06-01 00:00:00
abstract：:Hepatitis B virus (HBV) DNA integrates into the host DNA and shows a series of potentially oncogenetic properties, but HBV is not an acutely transforming virus, because HCC develops decades after infection. Other factors, namely cirrhosis, inflammation, alcohol intake, and viral superinfections, could promote the onco...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01297460
更新日期：1991-08-01 00:00:00
abstract：BACKGROUND:Diabetic gastroparesis is a common complication of diabetes mellitus, which mainly affects women. Previous studies have demonstrated that oxidative stress is involved in its onset and development. AIMS:This study evaluated the role of vitamin C on diabetes-associated gastric dysmotility. METHODS:Female rat...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-017-4632-9
更新日期：2017-09-01 00:00:00
abstract：AIM:Several models of experimental ulcerative colitis have been previously reported. To date, only one model of Crohn's colitis that was induced by trinitrobenzene sulfonic acid has been described. MATERIALS AND METHODS:Twenty-one male albino rats were divided into three groups: group I: sham control group (n = 7), gr...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1697-8
更新日期：2011-10-01 00:00:00
abstract：:The potential effect of recombinant human interleukin-11 (rhIL-11) on trinitrobenzene sulfonic acid (TNB)-induced colitis was investigated in rats. Intrarectal TNB (40 mg in 0.25 ml 40% ethanol) produced significant ulcerative colitis. The lesions were most severe at three days after TNB instillation, and then decline...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02087911
更新日期：1996-08-01 00:00:00
abstract：:We studied intraesophageal pressure changes in patients with symptoms of gastroesophageal reflux and an abnormal 24-hr pH monitoring record (N = 52). Our method was simultaneous esophageal manometry and pH monitoring. We observed a three-component esophageal manometric sequence (EMS). When this sequence recurred over ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01538066
更新日期：1989-09-01 00:00:00
abstract：:Up to 60% of patients with IBS have lowered perception thresholds in the rectum to balloon distension. The current study sought to test the hypothesis that IBS patients with normal perception thresholds in the rectum show hypersensitivity of afferent pathways in the sigmoid colon. Eleven healthy normal subjects and ei...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1018817132213
更新日期：1997-06-01 00:00:00
abstract：:The study investigated the effect of either nocturnal acid suppression by the H2 antagonist nizatidine 300 mg at night or prolonged acid suppression by the proton- pump inhibitor omeprazole 20 mg in the morning, during four weeks, on intragastric pH profile, occurrence of bacterial growth in gastric fluid and biopsies...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02093609
更新日期：1996-10-01 00:00:00
abstract：UNLABELLED:The purpose of this study was to investigate and compare the specificity, sensitivity, and area under curve (AUC) of the lactulose/mannitol ratio, lactulose/creatinine ratio, and lactulose recovery and their diagnostic value for intestinal permeability assessment within the absorption lactulose/mannitol (L/M...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-007-0184-8
更新日期：2008-10-01 00:00:00
abstract：:A variety of human disease conditions are associated with chronic intestinal disorders or enteropathies that are characterized by intestinal inflammation, increased gut permeability, and reduced capacity to absorb nutrients. Such disruptions in the homeostasis of the gastrointestinal (GI) tract can lead to symptoms of...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-014-3322-0
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Dextran sodium sulphate (DSS) is commonly used to induce intestinal inflammation in rodents. Despite its continuing importance as a model system for examining IBD pathogenesis, the mucosal and systemic immune responses have not been comprehensively documented. AIMS:The purpose of this study was to dissect f...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-010-1240-3
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:This study aimed to objectively investigate whether the addition of polydextrose to sterilized probiotic containing Lactobacillus helveticus will confer benefits to constipation-predominant irritable bowel syndrome patients. METHODS:A total of 163 patients were randomized into two groups: Group A to consume...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10620-019-05695-3
更新日期：2020-02-01 00:00:00
abstract：:Patients with previous gastric surgery for benign conditions are thought to be at increased risk for gastric cancer based on studies from Europe. Recommendations have been made to screen these patients repeatedly in order to detect early gastric cancers. We conducted a case-control study to estimate the risk in the Un...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01312941
更新日期：1984-08-01 00:00:00
abstract：:Concentration and voltage dependence of Mg transport across the rat duodenum was measured in an Ussing chamber. Mucosa (m) to serosa (s) Mg flux exhibits a cellular fraction comparable to that found for Ca. Mg sm flux is purely diffusive and probably restricted to the paracellular pathway. At all concentrations betwee...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01296406
更新日期：1991-11-01 00:00:00
abstract：:The original version of the article unfortunately contained an error in the surname of the second author, Dr. Ferdinando D'Amico in the author group. ...
journal_title：Digestive diseases and sciences
pub_type： 已发布勘误
doi：10.1007/s10620-020-06613-8
更新日期：2020-09-25 00:00:00
abstract：:The aim of this study was to examine the seroprevalence of antibody to CagA antigen, as well as other major H. pylori antigens by Western blot in H. pylori-infected subjects with endoscopically normal mucosa (N = 54) and duodenal ulcer (N = 51). The role of the host and environmental factors was also evaluated. There ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1026634721351
更新日期：1999-10-01 00:00:00
abstract：:Colonic diverticula have been generally accepted as a source of massive hemorrhaging. Little is known, however, about fecal occult blood loss from colonic diverticula and diverticulosis. We retrospectively investigated the possibility of minor bleeding in 737 diverticula cases diagnosed from April 1989 to May 1994. Th...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1018866915782
更新日期：1998-07-01 00:00:00
abstract：:Octreotide is a crucial drug used for treating patients with chylous ascites; however, there have been few reports related to octreotide that are being used in cirrhotic patients. Thus, this thesis is designed to determine the effects of octreotide on patients with chylous ascites after liver cirrhosis. Eight patients...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-008-0566-6
更新日期：2009-08-01 00:00:00
abstract：:A previous study showed that supine gastroesophageal reflux occurs mainly in the early nighttime period and is greater if the patient retires within 2 hr of a meal. We analyzed 306 consecutive esophageal pH studies between January and July 2003. Ninety-two patients had abnormal supine reflux. The recumbent period was ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-006-9707-y
更新日期：2007-10-01 00:00:00
abstract：BACKGROUND:Controlled attenuation parameter (CAP) has been developed to estimate the extent of hepatic steatosis. AIMS:The purpose was to evaluate the usefulness of CAP in assessing hepatic steatosis and its change using magnetic resonance imaging proton density fat fraction (MRI-PDFF) as reference standard. METHODS:...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05883-1
更新日期：2020-05-01 00:00:00
