Abstract：
:The C-terminus of the transcription factor p53 seems to play an important role by controlling the specific DNA-binding activity, which is directly associated with sensing damaged DNA. Another region located in the N-terminus of the protein has also been shown to regulate the DNA-binding activity of the protein. This activity can be promoted by peptides derived from these two negative regulatory regions or by binding of antibodies directed against the C-terminus of the p53 protein. Using both phage display peptide and multiple peptide synthesis technologies, we demonstrated that mAbs HR231 and Pab421, two p53-activating antibodies, recognize peptides derived from the C-terminus of p53, as previously described, but also peptides from the N-terminus of the protein, suggesting that these peptides are part of a conformational epitope. Furthermore, the sequences of these peptides are located in the two negative regulatory regions identified on the p53 protein, which is consistent with the biological activity of mAbs HR231 and Pab421.
journal_name
Peptidesjournal_title
Peptidesauthors
Portefaix JM,Rio MD,Granier C,Roquet F,Pau B,Navarro-Teulon Idoi
10.1016/s0196-9781(03)00047-0subject
Has Abstractpub_date
2003-03-01 00:00:00pages
339-45issue
3eissn
0196-9781issn
1873-5169pii
S0196978103000470journal_volume
24pub_type
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