Abstract：
:We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) epsilon chain, p56(lck), Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR epsilon chain and p56(lck) expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P<0.001), FasL expression was detected in 45% cells from melanomas (P<0.001) and in 3% from colorectal carcinomas (P=0.09), and Bax-positive cells increased from 17.5 to 70% (P=0.005). Moreover, TCR zeta chain-positive cells were significantly increased from baseline (P=0.001), Bcl-2-positive cells dropped from 50 to 1% (P=0.007) and perforin content was high, while Fas expression was not significantly modified by IL-2 culture. In conclusion, our data suggest that the degree of immunosuppression in TIL from melanomas and colorectal carcinomas is very high, and the apoptosis markers' repertoire of cancer cells resembles that of immune-privileged tissue. Interleukin-2 culture appears to restore lymphocyte activation mechanisms, resulting in consistent FasL expression and perforin production.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
De Paola F,Ridolfi R,Riccobon A,Flamini E,Barzanti F,Granato AM,Mordenti GL,Medri L,Vitali P,Amadori Ddoi
10.1038/sj.bjc.6600679subject
Has Abstractpub_date
2003-01-27 00:00:00pages
320-6issue
2eissn
0007-0920issn
1532-1827pii
6600679journal_volume
88pub_type
杂志文章abstract：BACKGROUND:The association between gall bladder removal (cholecystectomy) and hepatocellular carcinoma warrants investigation. An increased intrahepatic bile duct pressure following cholecystectomy might cause chronic inflammation in the surrounding liver tissue, which might induce cancer development. METHODS:A nation...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.181
更新日期：2011-06-28 00:00:00
abstract：:The aims of this study were to identify prognostic variables for toxicity and survival in patients with cancer participating in phase I clinical trials and compare characteristics of those treated with cytotoxic chemotherapy (CT) and non-cytotoxic drugs (non-CT). Data were collected from 420 (114 CT, 306 non-CT) patie...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601218
更新日期：2003-10-06 00:00:00
abstract：:Serum levels of pepsinogen and gastrin are parameters that can be used as biomarkers for gastric mucosa. The aim of this study was to validate these serum biomarkers, that is pepsinogen A (PGA), pepsinogen C (PGC), PGA/PGC ratio, and gastrin, as screening tests for precancerous lesions: atrophic chronic gastritis (ACG...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600877
更新日期：2003-04-22 00:00:00
abstract：BACKGROUND:Patients with malignant pleural effusions (MPEs) generally have advanced disease with poor survival and few therapeutic options. Cells within MPEs may be used to stratify patients for targeted therapy. Targeted therapy with poly(ADP ribose) polymerase inhibitors (PARPi) depends on identifying homologous reco...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.261
更新日期：2014-07-08 00:00:00
abstract：BACKGROUND:The 1997 international consensus conference on renal cell cancer (RCC) prognosis suggested erythrocyte sedimentation rate (ESR), alkaline phosphatase (ALP), and anaemia as prognostic biomarkers, but most studies reviewed were limited by small sample sizes. METHODS:The Cox proportional hazards model was used...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.565
更新日期：2013-02-05 00:00:00
abstract：:Deregulated expression of myc proto-oncogenes is implicated in several human neoplasias. We analysed the expression of c-myc, N-myc, L-myc, max and RB1 mRNAs in a panel of human gliomas and glioma cell lines and compared the findings with normal neural cells. The max and RB1 genes were included in the study because th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.3
更新日期：1994-01-01 00:00:00
abstract：:A partially purified fraction of the water soluble photosensitive dye sulphonated aluminium phthalocyanine (AlSPc) was encapsulated in liposomes which were then linked to a targeting monoclonal antibody 791T/36 using a heterobifunctional linking agent. The photocytotoxic effects of the liposomes were determined on two...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1989.73
更新日期：1989-03-01 00:00:00
abstract：:There are two main histological groups of testicular germ cell tumours, which may have different risk factors. Some authors have analysed potential risk factors by histological group but few consistent differences have been identified. In this paper we examine risk factors for pure seminoma and other tumours using dat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690611
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:We evaluated the efficacy of aprepitant plus granisetron and an increased dose of dexamethasone in selected patients undergoing moderately emetogenic chemotherapy (MEC). METHODS:Nondrinking women <70 years undergoing MEC were randomly assigned to aprepitant (day 1, 125 mg; days 2 and 3, 80 mg) or placebo. D...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2013.400
更新日期：2013-08-20 00:00:00
abstract：:The inability to assess the extent of tumour damage immediately following treatment is a major clinical obstacle to improving the management of cancer patients. Normally, the effectiveness of chemotherapy or radiation therapy cannot be determined for at least several weeks after treatment. We studied the increase in m...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.455
更新日期：1994-12-01 00:00:00
abstract：:The aim of this work was to test in vitro and in vivo the efficacy of the derivatives of 5-aminolevulinic acid (ALA): hexyl-ALA (He-ALA), undecanoyl-ALA and R,S-2-(hydroximethyl)tetrahydropyranyl-ALA (THP-ALA) as pro-photosensitising agents. The compounds were assayed in a cell line derived from a murine mammary tumou...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601722
更新日期：2004-04-19 00:00:00
abstract：:We present the cytogenetic investigations of five histiocytic tumour lesions from children. In four cases there was a confirmed diagnosis of Langerhans cell histiocytosis (LCH) and one case of histiocytosis that did not fulfil all the criteria for true LCH. All five cases showed cytogenetic abnormalities, including th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.89
更新日期：1998-02-01 00:00:00
abstract：:Two subtypes of thrombospondin (TSP-1 and TSP-2) have inhibitory roles in angiogenesis in vitro, although the biological significance of these TSP isoforms has not been determined in vivo. We examined TSP-1 and TSP-2 gene expression by reverse transcription polymerase chain reaction (RT-PCR) analysis in 61 colon cance...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690056
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Anti-EGFR antibody, cetuximab, improves overall survival (OS) in K-ras wild-type chemotherapy-refractory colorectal cancer. Epidermal growth factor receptor ligand epiregulin (EREG) gene expression may further predict cetuximab benefit. METHODS:Tumour samples from a phase III clinical trial of cetuximab plu...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.753
更新日期：2014-02-04 00:00:00
abstract：:Peripheral blood mononuclear cells (PBMCs) were obtained from patients receiving radioactive murine monoclonal antibody (MAb) therapy for malignant epithelial tumours, as well as normal controls, and were tested for the ability of T cells to proliferate in vitro in the presence of the MAb administered for therapy (HMF...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.337
更新日期：1991-09-01 00:00:00
abstract：:Protein kinases play a key role in intracellular signalling, participating at multiple levels along the transduction cascades that trigger mitogenic response. Because protein kinases are involved in mitogenic pathways, they are likely to play a role in the abnormal proliferation of malignant cells. In this study we co...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.675
更新日期：1998-11-01 00:00:00
abstract：:The orally administered tubulin-binding agent 1069C85 was developed with the hope of overcoming the multidrug resistance associated with existing anti-tubulin agents, such as the vinca alkaloids. A phase I study was performed using a single oral dose every 3 weeks, administered as a suspension reconstituted in 0.1% Tw...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1997.107
更新日期：1997-01-01 00:00:00
abstract：:Non-small-cell lung cancer (NSCLC) prognosis is strictly related to well-established clinicopathological parameters which have unfortunately become insufficient in the prognostic evaluation of this type of cancer. As p53 and bcl-2 gene deregulations are frequently involved in several types of epithelial malignancies, ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.193
更新日期：1995-05-01 00:00:00
abstract：:Imaging has an essential role in the planning and delivery of radiotherapy. Recent advances in imaging have led to the development of advanced radiotherapy techniques-including image-guided radiotherapy, intensity-modulated radiotherapy, stereotactic body radiotherapy and proton beam therapy. The optimal use of imagin...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/s41416-019-0412-y
更新日期：2019-04-01 00:00:00
abstract：:Bryostatin 1 is a novel antitumour agent derived from Bugula neritina of the marine phylum Ectoprocta. Nineteen patients with advanced solid tumours were entered into a phase I study to evaluate the toxicity and biological effects of bryostatin 1. Bryostatin 1 was given as a one hour intravenous infusion at the beginn...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1993.352
更新日期：1993-08-01 00:00:00
abstract：BACKGROUND:We evaluated the association between smoking status and thyroid cancer risk and whether this association is mediated by body mass index (BMI) and thyroid-stimulating hormone (TSH). METHODS:We performed a cohort study of 96,855 Korean adults who were followed annually or biennially for a median of 5.9 years....
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-018-0224-5
更新日期：2018-08-01 00:00:00
abstract：:The hybrid cell line BH2 was derived by fusion between an AKR thymoma BW5147 (HGPRT-) and C57BL thymoma EL-4R (TK-). The hybrid cells showed a near-tetraploid modal number of chromosomes, in contrast to the near-diploid stem-lines of both parental cell populations; most of the BH2 hybrid cells acquired marker chromoso...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.255
更新日期：1981-11-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：British journal of cancer
pub_type： 已发布勘误
doi：10.1038/s41416-020-0830-x
更新日期：2020-05-01 00:00:00
abstract：:Maturation of monocytes into macrophages in vitro has been assayed by a quantitative microtitre assay in patients with squamous-cell carcinoma of the lung. Monocyte maturation in autologous serum was significantly depressed in patients with both limited and extensive disease compared with normal controls, patients wit...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.71
更新日期：1981-04-01 00:00:00
abstract：BACKGROUND:Currently, none of the available colorectal adenocarcinoma (CAC) testing has been established as a well-accepted diagnosis tool, particularly for the early stage of CAC. The recent discovery of serum microRNA (miRNA) profile has provided a new auxiliary approach for tumour diagnosis. Our study is involved in...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.489
更新日期：2014-11-11 00:00:00
abstract：:Dendritic cells (DC) are professional antigen-presenting cells that play a pivotal role in the induction of immunity. Ex vivo-generated, tumour antigen-loaded mature DC are currently exploited as cancer vaccines in clinical studies. However, antigen loading and maturation of DC directly in vivo would greatly facilitat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603341
更新日期：2006-10-09 00:00:00
abstract：:An antigen common to human sarcomas, S3, has been further characterized. It is antigenically distinct from human blood-group substances A and B and from heterophile antigens such as Forssman, infectious mononucleosis and serum sickness antigens. Whilst S3 antigen preparations may contain small amounts of CEA and AFP t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.43
更新日期：1981-03-01 00:00:00
abstract：:Previous studies have shown that multidrug resistance (MDR) in the doxorubicin-selected lung tumour cell lines COR-L23/R, GLC4/ADR and MOR/R is associated with overexpression of the MRP gene. In this study we report that resistance to daunorubicin, vincristine and rhodamine 123 can be partially reversed in these cell ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.281
更新日期：1995-07-01 00:00:00
abstract：:Foetal acinar components associated with the development of the hamster pancreas have been previously defined with the aid of an antifoetal pancreas serum. In immunohistology this antiserum also stained malignant ductal cells in N-nitrobis (2-oxopropyl) amine (BOP)-induced pancreatic adenocarcinoma. It did not stain a...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1985.28
更新日期：1985-02-01 00:00:00
abstract：:In the present study we have used flow cytometric DNA measurements on synchronised human NHIK 3025 cells to measure cell cycle progression under various conditions of reduced oxygenation. Our data indicate that addition of 0.1 mM deoxycytidine or uridine has no effect on the oxygen-dependent arrest in late G1 or on th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.411
更新日期：1994-11-01 00:00:00
