Abstract:
:In the present study we have used flow cytometric DNA measurements on synchronised human NHIK 3025 cells to measure cell cycle progression under various conditions of reduced oxygenation. Our data indicate that addition of 0.1 mM deoxycytidine or uridine has no effect on the oxygen-dependent arrest in late G1 or on the inhibition of cell proliferation through S-phase under extremely hypoxic conditions. Following reoxygenation of cells exposed to extremely hypoxic conditions in G2 initiation of DNA synthesis in the subsequent cell cycle is delayed by several hours. This G2-induced delay is completely abolished for approximately 60% of the cell population by addition of deoxycytidine to hypoxic G2 cells. This finding supports our previous proposal that important steps in the preparation for DNA synthesis occur during G2 of the previous cell cycle, and it indicates that this preparation is connected to the de novo synthesis of pyrimidine deoxynucleotide precursors. The results show that cells are able to enter S-phase in the presence of 100-1,300 p.p.m. (0.01-0.13%) oxygen (here denoted 'moderate hypoxia'), but they are not able to complete DNA synthesis under such conditions. However, the cell cycle inhibition induced under moderate hypoxia is partially reversed in the presence of exogenously added deoxycytidine and uridine, while no such reversal is seen in the presence of purine deoxynucleosides (deoxyadenosine and deoxyguanosine). Thus, both deoxycytidine and uridine could replace reoxygenation under these conditions. These results indicate that the reduction of CDP to dCTP by ribonucleotide reductase, an enzyme which requires oxygen as an essential factor for the formation of tyrosyl radicals for its catalytic activity, does not seem to be the limiting step responsible for the reduced dCTP pool observed under moderate hypoxia. We conclude that the oxygen-dependent catalytic activity of the M2 subunit of ribonucleotide reductase is still intact and functional in NHIK 3025 cells even at oxygen concentration as low as 100 p.p.m. Therefore the cell cycle inhibition observed is probably due to inhibition of the respiratory chain-dependent UMP synthesis at the stage of dihydroorotate dehydrogenase.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Amellem O,Löffler M,Pettersen EOdoi
10.1038/bjc.1994.411subject
Has Abstractpub_date
1994-11-01 00:00:00pages
857-66issue
5eissn
0007-0920issn
1532-1827journal_volume
70pub_type
杂志文章abstract::The combination of 5-fluorouracil (5-FU) and interferon-alpha (IFN-alpha) has reported activity in the treatment of advanced colorectal carcinoma. Laboratory studies of IFN-beta suggest that this agent may offer theoretical advantages over IFN-alpha in combination with 5-FU. A total of 27 patients with advanced or rec...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1997.69
更新日期:1997-01-01 00:00:00
abstract::Complex biological pathways including angiogenesis, invasion, osteoclastic activation and bone matrix degradation are involved in the formation of bone metastasis (BM). The aim of our study was to investigate the cross-sectional and longitudinal associations of a panel of 12 serum biochemical markers reflecting biolog...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603285
更新日期:2006-08-21 00:00:00
abstract::Endogamous groups in Western India have been known to have a wide degree of genetic diversity. Analysis of the data available on some of the endogamous groups belonging to two main communities attending the Tata Memorial Hospital, Bombay, viz. Hindus from Maharashtra, and Hindus from Gujarat show that one of the endog...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1971.77
更新日期:1971-12-01 00:00:00
abstract:BACKGROUND:Pancreatic cancer is highly malignant and characterised by rapid and uncontrolled growth. While some of the important regulatory networks involved in pancreatic cancer have been determined, the cancer relevant genes have not been fully identified. METHODS:We screened genes that may control proliferation in ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2017.165
更新日期:2017-07-11 00:00:00
abstract::In rapidly frozen livers from chickens affected with myeloblastosis and Marek's disease and from unaffected control birds there exists a strong correlation between catalase activity and catalase Electron Paramagnetic Resonance (EPR) signal intensities. The diseased chickens had activities and signals reduced to as lit...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1984.189
更新日期:1984-09-01 00:00:00
abstract::We investigated the predictors of the risk of developing a second primary cancer after breast cancer, this occurring in about 12% of affected women. The analysis included 335 191 females, registered in the National Cancer Institute's Surveillance Epidemiology and End Results (SEER) database, who had been diagnosed wit...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603172
更新日期:2006-06-05 00:00:00
abstract::Activation of Met by its ligand HGF has been shown to elicit both mitogenic and motogenic responses in thyrocytes in vitro. In the present study we have investigated the expression of Met in human anaplastic thyroid carcinoma cells in culture. There was a variation in expression level and size of Met in the different ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690406
更新日期:1999-05-01 00:00:00
abstract::Counter-irritation (CI) triggered by different non-specific irritant stimuli delayed the growth of a murine tumour of non-detected immunogenicity. The syngeneic LB tumour transplant by itself also induced CI and decreased the number of leukocytes migrating to a secondary s.c. irritant stimulus, e.g. sponge or carragee...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.349
更新日期:1989-11-01 00:00:00
abstract::The effect of 6 putative prognostic factors on survival was studied in patients with Stages III and IV malignant teratoma of the testis. Differences between survival curves were tested for statistical significance. A diameter greater than 5 cm in the largest tumour mass, and greater than 8 pulmonary metastases were ad...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.332
更新日期:1980-12-01 00:00:00
abstract::Erbicin is a human anti-ErbB2 single-chain antibody fragment with high affinity and selectivity for ErbB2-positive cancer cells. Two anti-ErbB2 immunoconjugates, called Erb-hRNase and Erb-hcAb, have been prepared and found to be selectively cytotoxic on ErbB2-positive cancer cells in vitro and vivo. In Erb-hRNase, Erb...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604022
更新日期:2007-11-19 00:00:00
abstract::The present study was designed to examine whether different p53 haplotypes of exon 4-intron 3-intron 6 affect the frequency of mutations and loss of heterozygosity (LOH) of the p53 gene in male oral squamous cell carcinomas (OSCCs) in Taiwan. We found that individuals without two Pro-W-G alleles had significantly high...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602271
更新日期:2005-01-17 00:00:00
abstract:BACKGROUND:Melatonin induces apoptosis in many different cancer cell lines, including hepatocellular carcinoma cells. However, the responsible pathways have not been clearly elucidated. A member of the forkhead transcription factors' family, FoxO3a, has been implicated in the expression of the proapoptotic protein Bim ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.563
更新日期:2013-02-05 00:00:00
abstract:BACKGROUND:Neuroblastoma is the most common malignancy of infancy but little is known about the aetiological factors associated with the development of this tumour. A number of epidemiological studies have previously examined the risk associated with paternal occupational exposures but most have involved small numbers ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605504
更新日期:2010-02-02 00:00:00
abstract::It remains unclear whether any aspect of quality of life has a role in predicting survival in an unselected cohort of patients with gastro-oesophageal cancer. Therefore the aim of the present study was to examine the relationship between quality of life (EORTC QLQ-C30), clinico-pathological characteristics and surviva...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604248
更新日期:2008-03-11 00:00:00
abstract:BACKGROUND:Robot-assisted radical prostatectomy (RARP) has been rapidly adopted without robust evidence comparing its functional outcomes against laparoscopic radical prostatectomy (LRP) or open retropubic radical prostatectomy (ORP) approaches. This study compared patient-reported functional outcomes following RARP, L...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2017.454
更新日期:2018-02-20 00:00:00
abstract::We developed a decision aid (DA) for patients with metastatic non-small cell lung cancer (NSCLC), to better inform patients of their prognosis and treatment options, and facilitate involvement in decision-making. In a pilot study, 20 patients with metastatic NSCLC attending outpatient clinics at a major cancer centre,...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604395
更新日期:2008-06-03 00:00:00
abstract::Capecitabine is an oral prodrug of 5-fluorouracil (FU). Since FU concentrations achieved in malignant lesions are an important determinant of efficacy, we investigated the intratumoral transcapillary transfer of capecitabine and its metabolites in vivo. A total of 10 patients with skin metastases from breast cancer re...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600809
更新日期:2003-03-10 00:00:00
abstract::Vascular endothelial growth factor-C (VEGF-C) functions specifically to induce lymphangiogenesis. We examined the relationship between expression of VEGF-C and clinicopathological features in patients with colorectal cancer. The expression of VEGF-C in the 99 primary tumours and 18 metastatic lymph nodes from colorect...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2000.1396
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND:The 1997 international consensus conference on renal cell cancer (RCC) prognosis suggested erythrocyte sedimentation rate (ESR), alkaline phosphatase (ALP), and anaemia as prognostic biomarkers, but most studies reviewed were limited by small sample sizes. METHODS:The Cox proportional hazards model was used...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.565
更新日期:2013-02-05 00:00:00
abstract:BACKGROUND:A combination of S-1 and cisplatin has been shown to be effective with acceptable safety for the first-line treatment of far-advanced gastric cancer in Japan. This is the first randomised phase II trial to compare S-1+paclitaxel with S-1+cisplatin in this setting. METHODS:Patients with unresectable and/or r...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2012.222
更新日期:2012-06-26 00:00:00
abstract::The present study set out to evaluate perceptions of risk, psychological morbidity and health behaviours in women with a family history of breast cancer who have attended genetic counselling and determine how these differ from general population risk women. Data were collected from 62 genetic counselees (cases) attend...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.387
更新日期:1996-08-01 00:00:00
abstract::Besides its cardiotoxic effect, doxorubicin also elicits inflammatory effects in vivo. 7-Monohydroxyethylrutoside (monoHER) has recently been used as a protector against doxorubicin-induced cardiotoxicity in vivo. It is not known yet whether monoHER can also protect against doxorubicin-induced inflammatory effects. Th...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601022
更新日期:2003-07-21 00:00:00
abstract::Hyperthermia (42° C.) exerted an inhibitory effect on the O(2) uptake of rabbit VX2 carcinoma cells in vitro, and led to a decrease in viability and growth potential of the cells, as measured by their ability to produce tumours in rabbits. Anaerobic glycolysis of the tumour cells was unaltered by hyperthermia. Respira...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1971.91
更新日期:1971-12-01 00:00:00
abstract::Circulating anti-p53 antibodies have been described and used as tumoural markers in patients with various cancers and strongly correlate with the p53 mutated status of the tumours. No study has yet looked at the prevalence of such antibodies in skin carcinoma patients although these tumours have been shown to be frequ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.2185
更新日期:2001-12-14 00:00:00
abstract::We have previously reported that supplementation with folic acid (1.2 mg day(-1) for 12 week) elicited a significant improvement in the folate status of 61 healthy volunteers. We have examined effects of this supplement on markers of genomic stability. Little is known about the effect of folate supplementation on DNA ...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/sj.bjc.6603197
更新日期:2006-06-19 00:00:00
abstract:BACKGROUND:Tumour carbonic anhydrase IX (CAIX), a hypoxia-inducible tumour-associated cell surface enzyme, is thought to acidify the tumour microenvironment by hydrating CO2 to form protons and bicarbonate, but there is no definitive evidence for this in solid tumours in vivo. METHODS:We used 1H magnetic resonance spe...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0216-5
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The urokinase plasminogen activator (uPA) system is one of the best-investigated protease systems, both under physiological and pathological conditions, including various types of cancer. However, effects of co-expression of members of the uPA system in soft-tissue sarcoma (STS) patients at the protein level...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605520
更新日期:2010-02-16 00:00:00
abstract:BACKGROUND:For patients with locally advanced rectal cancer (LARC), it is unclear whether neoadjuvant chemoradiotherapy-induced pathologic complete response (pCR) individuals would further benefit from adjuvant chemotherapy (ACT). METHODS:The pCR individuals who received different ACT cycles were paired by propensity ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0989-1
更新日期:2020-10-01 00:00:00
abstract::Kaposi's sarcoma (KS) is a highly vascular tumour and is the most common neoplasm associated with human immunodeficiency virus (HIV-1) infection. Growth factors, in particular vascular endothelial growth factor (VEGF), have been shown to play an important role in its development. The role of insulin-like growth factor...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602408
更新日期:2005-04-25 00:00:00
abstract::The relation between pretreatment night-time urinary catecholamine excretion and chemotherapy-induced nausea and vomiting was studied. The first cohort included 17 women and three men with various cancer forms receiving low or moderately emetogenic chemotherapy. The second cohort included 42 women receiving cisplatinu...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/bjc.1994.364
更新日期:1994-10-01 00:00:00