Abstract:
:Protein kinases play a key role in intracellular signalling, participating at multiple levels along the transduction cascades that trigger mitogenic response. Because protein kinases are involved in mitogenic pathways, they are likely to play a role in the abnormal proliferation of malignant cells. In this study we compared activity of mitogen-activated protein (MAP) kinase and several renaturable kinases in homogenates of 30 surgically resected colorectal cancers and their adjacent normal tissues. Using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and membrane autophosphorylation assay on homogenates obtained from normal colon mucosa and adenocarcinoma, we identified at least four renaturable kinases (50, 55, 85, 200 kDa). Compared with adjacent tissue, in most of the cancer samples only the 85-kDa kinase exhibited a higher level of autophosphorylation activity than those in normal matched tissue (P < 0.001). Moreover, the 85-kDa kinase from nearly all cancer homogenates showed faster electrophoretic mobility than the 85-kDa kinase from normal tissue homogenates. Interestingly, the 50-kDa kinase had significantly lower autophosphorylation activity in cancer tissues than those of normal tissue (P< 0.05). To assess p42-p44 MAP kinase activity, proteins were immunoprecipitated from adjacent colon mucosa and adenocarcinoma with anti-extracellular signal-related kinase (ERK) 1/2 antibodies, and MAP kinase activity was measured using MBP as a substrate. These studies revealed that MAP kinase activity in colorectal cancer was significantly higher (P < 0.001) than that in adjacent mucosa. Thus, the constitutive activity of MAP kinase and autophosphorylation activity of 85-kDa kinase are increased, whereas the autophosphorylation activity of another kinase, 50 kDa, is decreased in colorectal adenocarcinoma. However, although signal transduction pathways are markedly altered in this cancer, neither p42/p44 MAP kinase activity nor 85-kDa autokinase activity could be correlated with the established prognostic indicators.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Ostrowski J,Trzeciak L,Kołodziejski J,Bomsztyk Kdoi
10.1038/bjc.1998.675subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1301-6issue
10eissn
0007-0920issn
1532-1827journal_volume
78pub_type
杂志文章abstract::The Type 1 family of growth factor receptors includes epidermal growth factor receptor (EGFR), c-erbB-2, c-erbB-3 and c-erbB-4. Overexpression of the first two members is associated with poorer prognosis in patients with breast carcinoma. In this study we examined the expression of c-erbB-4 protein using the monoclona...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.1057
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:Tumoural interstitial hypertension, possibly modulated by platelet-derived and vascular endothelial growth factor receptors (PDGFR and VEGFR), may mediate resistance to chemotherapy. METHODS:Forty-eight patients with advanced solid tumours received oral PDGFR inhibitor CP-868,596 (60-100 mg twice daily (BID...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
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更新日期:2010-11-09 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.97
更新日期:1997-01-01 00:00:00
abstract::Testicular germ cell tumour (GCT) is not an AIDS-defining illness despite an increased incidence in men with HIV infection. We performed a matched case-control study comparing outcomes in HIV-positive men and the general population with GCT, using three age and stage matched controls for each case. There was no differ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601762
更新日期:2004-04-19 00:00:00
abstract::Patients with rectal cancer are at high risk of disease recurrence despite neoadjuvant radiochemotherapy with 5-Fluorouracil (5FU), a regimen that is now widely applied. In order to develop a regimen with increased antitumour activity, we previously established the recommended dose of neoadjuvant CPT-11 (three times w...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/sj.bjc.6603322
更新日期:2006-09-18 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.1997.87
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abstract::We investigated breast cancer incidence in migrants in the Netherlands in 1988-1998. The standardised incidence ratio for breast cancer in Northwest-Netherlands was statistically significantly reduced for women born in Surinam (0.56), Turkey (0.29) and Morocco (0.22). The proportion of women with advanced stages (III ...
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6601821
更新日期:2004-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690136
更新日期:1999-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-01254-0
更新日期:2021-01-21 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.459
更新日期:1996-09-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.137
更新日期:1997-01-01 00:00:00
abstract::To our knowledge, very few data about the role of Topoisomerase IIalpha (TOPO-IIalpha), an enzyme involved in critical steps of tumour cell proliferation and chemoresistance are currently available in ovarian cancer patients. The aim of this study was to investigate the prognostic value of TOPO-IIalpha expression in a...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604410
更新日期:2008-06-17 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690222
更新日期:1999-03-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.1013
更新日期:2000-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0081-2
更新日期:2018-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.1936
更新日期:2001-08-17 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2017.488
更新日期:2018-03-20 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.75
更新日期:1992-03-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1970.24
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.123
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0359-4
更新日期:2019-02-01 00:00:00
abstract::We investigated expression of a human p53-inducible gene, P2XM, a member of the P2X-receptor family of ATP-gated ion channels, in 56 human primary soft-tissue tumours including 47 sarcomas and nine benign tumors. Among the 47 sarcomas examined by reverse transcription polymerase chain reaction, 12 had lost expression ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690484
更新日期:1999-06-01 00:00:00
abstract::Question prompt lists (QPLs) have been shown to be an inexpensive and effective communication tool for patients in oncology consultations. We aimed to develop and pilot a QPL for palliative care (PC) patients. In order to identify suitable questions for inclusion in the QPL, we conducted focus groups and individual in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601380
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:Clear cell renal cancer frequently harbours von Hippel-Lindau (VHL) gene mutations, leading to stabilisation of the hypoxia-inducible factors (HIFs) and expression of their target genes. We investigated HIF-1 and HIF-2 in the regulation of microRNA-210 (miR-210), and its clinical relevance in renal tumours. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.56
更新日期:2013-03-19 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.2089
更新日期:2001-11-16 00:00:00
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pub_type: 杂志文章
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更新日期:2001-11-30 00:00:00
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pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600708
更新日期:2003-01-13 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.157
更新日期:1998-03-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690668
更新日期:1999-09-01 00:00:00