Abstract：
:Accumulation of beta-catenin in cytoplasm and nuclei is frequently observed in a wide variety of tumors arising, for example, in the colon, liver, uterus, or brain. In association with Tcf/LEF transcription factors, beta-catenin regulates expression of genes involved in the Wnt/wingless signaling pathway, but the precise mechanisms are unclear. Here we report evidence that the claudin-1 (CLDNI) gene is one of the genes regulated by beta-catenin. Not only did expression of CLDN1 decrease significantly in response to reduction of intracellular beta-catenin by adenovirus-mediated transfer of wild-type APC into the APC-deficient colon cancer cells, but also two putative Tcf4 binding elements in the 5' flanking region of CLDN1 were confirmed to be responsible for activating its transcription. We documented increased expression of CLDN1 in all 16 primary colorectal cancers we examined, compared with adjacent noncancerous mucosae. Furthermore, immunohistochemical staining demonstrated that claudin-1 was weakly stained at apical boarder of lateral membrane of noncancerous epithelial cells and that it was strongly stained at all cell-cell boundaries and in the cytoplasms of cancer cells. Our results imply that claudin-1 is involved in the beta-catenin-Tcf/LEF signaling pathway, and that increased expression of claudin-1 may have some role in colorectal tumorigenesis.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Miwa N,Furuse M,Tsukita S,Niikawa N,Nakamura Y,Furukawa Ydoi
10.3727/096504001108747477subject
Has Abstractpub_date
2001-01-01 00:00:00pages
469-76issue
11-12eissn
0965-0407issn
1555-3906journal_volume
12pub_type
杂志文章abstract：:Spinal osteosarcoma (OS) is a malignant tumor that has a poor outcome. MicroRNA-520b (miR-520b) acts as a cancer suppressor in various types of cancer. Because of the limited amount of literature on OS, we aimed to identify the role of miR-520b in OS. The miR-520b level in clinical spinal OS tissues and adjacent nontu...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14873430389189
更新日期：2017-09-21 00:00:00
abstract：:Astragaloside IV (AS-IV) is an active ingredient in Astragalus membranaceus and is involved in various biological processes, such as regulating the immune system, and counteracting inflammation and malignancy. The aim of this study was to explore the effect of AS-IV on non-small cell lung cancer (NSCLC) cells. Cell co...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15344989701565
更新日期：2019-03-29 00:00:00
abstract：:ErbB4 is a member of the ErbB family of receptor tyrosine kinases. Because of a paucity of appropriate pharmacologic tools, little is known about ErbB4 functions in vivo. In response to this need, we hypothesized that a recombinant form of the extracellular domain of ErbB4 would antagonize ligand-induced receptor tyro...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/0965040042707907
更新日期：2004-01-01 00:00:00
abstract：:Sixteen desmuramyl analogs of muramyl dipeptide (MDP) were tested for their abilities to stimulate cytotoxic factor secretion by mouse peritoneal macrophages. Among them, the pseudohexapeptide Boc-Gly psi [CH2O]-D-Ala-Ala-D-Glu[Lys(H-Gly)NHEt]-NH2 appeared to be four times more effective than MDP. From this study, the...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:PPAR-gamma is known to have a growth-suppressive effect in different cancers. In this study, we investigated the role of PPAR-gamma in the development of Barrett's esophagus (BE) and esophageal adenocarcinoma (EA). We used immunohistochemistry and real-time PCR to analyze differences in PPAR-gamma protein and mRNA exp...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504011x12935427587849
更新日期：2011-01-01 00:00:00
abstract：:Accumulated studies have strongly implicated aberrantly expressed microRNAs (miRNAs) in carcinogenesis and cancer progression of various cancers, including colorectal cancer (CRC). Hence, a comprehensive study of miRNAs and their association with CRC may be a promising therapeutic method for patients with this maligna...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15188747585738
更新日期：2018-10-17 00:00:00
abstract：:Cervical cancer is one of the most common neoplastic diseases affecting women, with a combined worldwide incidence of almost half a million new cases. Considering the fact that IL-18 plays an important role in the interactions among T cells, NK cells, and macrophages and induces IFN-gamma production, efforts should be...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008785114156
更新日期：2008-01-01 00:00:00
abstract：:Medullary thyroid carcinoma (MTC) is an uncommon and highly aggressive tumor of the neuroendocrine system, which derives from the neuroendocrine C cells of the thyroid gland. Except for surgical resection, there are not very many effective systemic treatment options for MTC. N-Myc downstream-regulated gene 2 (NDRG2) h...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504015X14452563486093
更新日期：2016-01-21 00:00:00
abstract：:Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) are used as first-line agents for treating nonsquamous cell lung cancer with EGFR mutation, there are many patients who have to receive these drugs following platinum-based chemotherapy. This study was designed to define whether exposure ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504012x13285365944337
更新日期：2011-01-01 00:00:00
abstract：:Apoptosis proteins may play a role in prognosis and therapy response; however, they have not been fully investigated in gastric cancer. We aimed to assess the expression of proteins in the Bcl-2 family. Immunohistochemistry was employed to examine the expression of the antiapoptotic proteins Bcl-2 and Bcl-XL and the p...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504005776568246
更新日期：2006-01-01 00:00:00
abstract：:The effects of treatment of HeLa cells with the antitumor nucleoside analog 2'-deoxy-2'-(fluoromethylene) cytidine (FMdC) on ultraviolet and X-ray sensitivity were examined. A 1-h pretreatment with FMdC increased cellular sensitivity to both X-rays and ultraviolet irradiation as determined by survival curve analysis. ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The regular RIN1 gene is a molecule located on chromosome 1lq13.2, and contains a coding region of 2352 bp with a 3' domain that binds to H-Ras protein, suggesting that it is an important molecule in the intracellular signaling pathway. In this study, we confirmed the existence of a novel form of the RIN1 gene with a ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009789745566
更新日期：2009-01-01 00:00:00
abstract：:Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14666990347671
更新日期：2016-01-01 00:00:00
abstract：:Venous thromboembolism (VTE) occurs at an increased incidence in cancer patients. A cancer-related hypercoagulable state has been considered to play role in this phenomenon. Preclinical data suggest an association between tumor expression of MET proto-oncogene (MET) and a hypercoagulable state, resulting in VTE. We in...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504010x12828372551786
更新日期：2010-01-01 00:00:00
abstract：:The patterns of DNA fragmentation were evaluated following a brief exposure (2 h) of the human ileocecal adenocarcinoma cell line, HCT-8, to several specific thymidylate synthase inhibitors, a quinazoline (ZD1694) and benz[cd]indole-containing molecule (AG-331). The magnitude and size of DNA fragmentation induced by t...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) ranks as the sixth most frequent cancer worldwide. HNSCC cell lines are typically refractory to transforming growth factor-beta (TGF-beta)-mediated cell cycle arrest. A number of these cell lines carry inactivating mutations of the TGF-beta type II (TbetaR-II) receptor, an...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000003108748612
更新日期：2003-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC ce...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14685034103950
更新日期：2016-10-27 00:00:00
abstract：:To investigate the effect of Kim-1 on 786-0 cells in vivo and in vitro, several experiments such as quantitative real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with Kim-1 siRNA. Furthermore, the tumor xenograft model was ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15140544654946
更新日期：2018-08-23 00:00:00
abstract：:We previously reported that 9-methylstreptimidone, a piperidine compound isolated from a culture filtrate of Streptomyces, induces apoptosis selectively in adult T-cell leukemia cells. It was screened for a compound that inhibits LPS-induced NF-kappaB and NO production in mouse macrophages. However, 9-methystreptimido...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504012x13425470196056
更新日期：2012-01-01 00:00:00
abstract：:A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist showed cell death in th...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14721731148893
更新日期：2017-04-14 00:00:00
abstract：:Our recent studies have shown that vanadium, a dietary micronutrient, has an inhibitory response against experimentally induced rat liver carcinogenesis. In the present study, the effect of vanadium on hepatic xenobiotic biotransformation in rats exposed to diethylnitrosamine (DENA, 200 mg/kg, IP) was investigated to ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Carbonic anhydrase (CA) IX has emerged as a promising target for cancer therapy. It is highly upregulated in hypoxic regions and mediates pH regulation critical for tumor cell survival as well as extracellular acidification of the tumor microenvironment, which promotes tumor aggressiveness via various mechanisms, such...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14965111926391
更新日期：2018-03-05 00:00:00
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journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Human papillomavirus type 16 (HPV16) is a major causative factor in the development of uterine cervical carcinomas. We investigated the role of E6/E7 in tumor formation. Skin-specific E6/E7 transgenic mice showed approximately twice as many tumors compared with nontransgenic mice in dimethylbenz[a]anthracene (DMBA)-in...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000006783980964
更新日期：2007-01-01 00:00:00
abstract：:Protein disulfide isomerases A6 (PDIA6) belongs to the PDI family. Recently, PDIA6 was found to have a close association with various cancers. However, there has been little investigation into the biological functions of PDIA6 in bladder cancer (BC). In this study, we explored the expression pattern and functional sig...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14761811155298
更新日期：2017-04-14 00:00:00
abstract：:MicroRNAs (miRNAs) play important roles in the carcinogenesis of cervical cancer. However, the expression and underlying mechanisms of miRNA in cervical cancer progression remain unclear. In the present study, our data showed that the expression of miR-138-5p was significantly downregulated in cervical cancer tissues,...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15017209042610
更新日期：2018-04-10 00:00:00
abstract：:Cancer-associated fibroblasts (CAFs) play a predominant role in regulating tumor progression. Understanding how CAFs communicate with osteosarcoma is crucial for developing novel approaches for osteosarcoma therapy. Exosomes are able to transmit messages between cells. In this study, we demonstrated that CAFs transfer...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15336368805108
更新日期：2019-09-23 00:00:00
abstract：:Epidermal growth factor receptor (EGFR) is commonly overexpressed in non-small cell lung cancer (NSCLC) and its tyrosine kinase phosphorylation is thought to be an ideal target in the treatment of patients with NSCLC. In the present study, we examined surgically obtained specimens from a series of 36 NSCLC patients fo...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504003108748348
更新日期：2003-01-01 00:00:00
abstract：:HL-60 cells isolated for resistance to vincristine are multidrug resistant and defective in the cellular accumulation of drug. Further studies demonstrate that these cells are also highly defective in 12-O-tetradecanoylphorbol-13-acetate (TPA) induced differentiation to macrophages. Analysis of this system demonstrate...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Phosphorothioate oligonucleotides have a potential as therapeutic agents. The pharmacokinetics, tissue distribution, stability, and cellular uptake by LOX ascites tumor of p120 antisense phosphorothioate oligonucleotide, ISIS 3466, were studied in vivo. The oligonucleotide, which was quickly cleared from the circulati...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
