Selective removal of circulating immune complexes from patient plasma.

Abstract:

:The principle of a patient-specific immunoadsorber (PsIA) is demonstrated. Studies with model systems (HSA/anti-HSA) on immobilization, stability, and leakage form the basis for the presented fast-performance liquid chromatography (FPLC) and batch experiments, which were conducted using two different protein A adsorbers and autologous and heterologous PsIA systems. Experiments to determine the binding capacity of protein A adsorbers and PsIAs are described. In all experiments, the adsorption of plasma IgG, total protein, and C1q and C3d circulating immune complexes were measured. Plasma of patients with autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus) was investigated. Analysis was performed in both the initial plasma and the flow-through or supernatant. Results of the investigations using FPLC and batch experiments were compared. Autologous PsIA systems are suitable for the selective removal of elevated levels of circulating immune complexes in the plasma.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Kunkel S,Holtz M,Grossjohann B,Schuett W,Klinkmann H,Kong D,Yamada R,Kimura H,Matic GB,Ramlow W,Boeden HF

doi

10.1046/j.1525-1594.2002.06875.x

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

124-32

issue

2

eissn

0160-564X

issn

1525-1594

journal_volume

26

pub_type

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