Abstract:
:A nonhemorrhagic proteinase B-20 from the venom of Bitis arietans has been purified to apparent electrophoretic homogeneity by chromatography on Sephadex G-100, Q-Sepharose, and CM-cellulose. It has a molecular weight of 20 k Da as determined by size exclusion chromatography on Sephadex G-100 and migrated as a single 20-k Da band on SDS polyacrylamide. It has an optimum pH of 6-8 and is inactive at pH 4.0. EDTA and 1,10-phenanthroline strongly inhibited the enzyme suggesting it is a metalloenzyme. Also it is inhibited by antipain but is unaffected by trasylol, antitrypsin, and pepsptatin. Colombin, an identified active component of Aristolochia albida used in the treatment of snake poisoning, did not inhibit the protease activity. It lost over 90% of its activity in the presence of 0.5 microM Hg(2+) but the inhibition was completely blocked in the presence of 10 microM mercaptoethanol implicating sulfhydryl groups in the catalytic entity of the protein. The activity was also inhibited competitively by glutathione and cysteine with inhibition binding constants K(i) of 240 and 40 microM, respectively. The enzyme is unaffected by several divalent cations but activated by 1 mM Fe(3+). It had a prolyl endopeptidase and thermolysin-like activity. The enzyme displayed a fast acting alpha-fibrinolytic and delayed gamma-fibrinolytic activity when tested on human fibrinogen. The relevance of these findings is discussed.
journal_name
J Biochem Mol Toxicoljournal_title
Journal of biochemical and molecular toxicologyauthors
Nok AJdoi
10.1002/jbt.19subject
Has Abstractpub_date
2001-01-01 00:00:00pages
215-20issue
4eissn
1095-6670issn
1099-0461pii
10.1002/jbt.19journal_volume
15pub_type
杂志文章abstract::Chrysin (CH) or 5,7-dihydroxyflavone is a flavonoid present in various plants, bee propolis, and honey. Cyclophosphamide (CYP) is a chemotherapeutic drug, which is extensively used in the treatment of multiple human malignancies. In our study, we aimed to investigate the effects of CYP and CH on some metabolic enzymes...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22313
更新日期:2019-06-01 00:00:00
abstract::The present study was designed to elucidate the involvement of acid phosphatase (ACP) in metastasis and lactate dehydrogenase (LDH) as an immediate compensatory alleviation mechanism for energy stress in liver lesions induced by hexachlorocyclohexane in Swiss mice. Animals were continuously exposed to hexachlorocycloh...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21441
更新日期:2012-11-01 00:00:00
abstract::Hemolysis is the red blood cell abnormality most often associated with adverse effect of drug therapy. Drug-induced or drug-associated hyperglycemia could decrease the activity of hexokinase. The aim of this study was to investigate the inhibitory effects of some commonly used drugs that have hyperglycemic side effect...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21910
更新日期:2017-08-01 00:00:00
abstract::It has been recently postulated from our laboratory that Arabic gum (AG) offers a protective effect in the kidney of rats against nephrotoxicity induced by gentamicin via inhibiting lipid peroxidation. It has also recently shown a powerful antioxidant effect through scavenging superoxide anions. In this study we utili...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.10072
更新日期:2003-01-01 00:00:00
abstract::Allyl alcohol hepatotoxicity is mediated by an alcohol dehydrogenase-derived biotranformation product, acrolein. This highly reactive alpha,beta-unsaturated aldehyde readily alkylates model proteins in vitro, forming, among other products, Michael addition adducts that possess a free carbonyl group. Whether such damag...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.10007
更新日期:2001-01-01 00:00:00
abstract::Netrin-1 has been found to protect kidneys from ischemia/reperfusion injury. In this study, we aimed to address whether the protective effects were mediated through suppression of oxidative stress and neuropeptide Y. Compared to sham-operated animals, animals after ischemia/reperfusion showed marked kidney damage and ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21474
更新日期:2013-04-01 00:00:00
abstract::The present study investigated the protective effect of gossypol, selenium, zinc, or glutathione (GSH) against dimethylnitrosamine (DMN)-induced hepatotoxicity in the livers of male mice. The expression and the activity of glutathione S-transferase (GST), levels of GSH, and free radicals (malondialdehyde (MDA)), as we...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20255
更新日期:2008-11-01 00:00:00
abstract::Hyperglycemia, a major metabolic disturbance present in diabetes, promotes oxidative stress. Activation of antioxidant defense is an important mechanism to prevent cell damage. Levels of heavy metals and their binding proteins can contribute to oxidative stress. Antiradical capacity and levels of metallothionein (MT),...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,多中心研究
doi:10.1002/jbt.21891
更新日期:2017-06-01 00:00:00
abstract::Significant changes in drug-metabolizing enzyme (DME) expression occur during ontogeny. Such changes can have a profound effect on therapeutic efficacy in the fetus and child, as well as the risk for adverse drug reactions. To gain a better understanding of DME ontogeny, enzyme contents for six key cytochromes P450 we...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.20179
更新日期:2007-01-01 00:00:00
abstract::Chalcones and chalcone epoxides are important synthetic intermediates in organic and medicinal chemistry. Chalcones possess a broad spectrum of biological activities; however, 1,3-diphenyl-2-propenone or chalcone has not been given the attention it deserve as its substituted derivatives. In this study, the inhibition ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22240
更新日期:2019-02-01 00:00:00
abstract::Mitochondria play an important role in myocardial tissue homeostasis; therefore, deterioration in mitochondrial function will eventually lead to cardiomyocyte and endothelial cell death and consequently cardiovascular dysfunction. Lithium (Li+ ) is an effective drug for bipolar disorder with known cardiotoxic side eff...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21836
更新日期:2017-02-01 00:00:00
abstract::The purpose of our study is to assist in understanding the effects of wireless electromagnetic waves on carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes activities in the different tissues of the rats. For this purpose, two different groups each of which contains eight rats (n = 8) were formed as being ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22031
更新日期:2018-03-01 00:00:00
abstract::Casearia esculenta root (Roxb.) is widely used in traditional system of medicine to treat diabetes in India. An active compound, 3-hydroxymethyl xylitol (3-HMX), has been isolated, and its optimum dose has been determined in a short duration study and patented. In addition, the long-term effect of 3-HMX in type 2 diab...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20317
更新日期:2010-03-01 00:00:00
abstract:UNLABELLED:Cellular induction of reductase enzymes can alter the susceptibility of cells toward drugs and chemicals. In this study, we compared the capacity of a single dose of sodium selenite and 3H-1,2-dithiole-3-thione (D3T) to influence the drug-relevant reducing capacity of HT29 cells over time, and defined the pr...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21601
更新日期:2015-01-01 00:00:00
abstract::Changes in the chemical or physical conditions of the cell that impose a negative effect on growth demand rapid cellular responses, which are essential for survival. Molecular mechanisms induced upon exposure of cells to such adverse conditions are commonly designated as stress responses. Herein, different methods whi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.20091
更新日期:2005-01-01 00:00:00
abstract::Recent studies have established the importance of estrogen-related receptor γ (ERRγ) as a required participant for insulin secretion in pancreatic β cells. Key downstream genes of ERRγ remain unclear in the pancreatic β cell. To understand the molecular role of ERRγ and elucidate potential key candidate genes involved...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22390
更新日期:2019-10-01 00:00:00
abstract::Certain ginsenosides, also known as triterpene glycosides, have been recently reported to have a characteristic effect on cultured intestinal and leukemia cell growth. Ginsenoside aglycones 20(S)-protopanaxadiol (PD), 20(S)-protopanaxatriol (PT), and ginsenoside Rh2 have been identified as having a strong effect on re...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20019
更新日期:2004-01-01 00:00:00
abstract::The fluoroquinolones absorb light in the 320 to 330 nm ultraviolet A (UV-A) wavelength and produce reactive oxygen species (ROS) such as superoxide anion, hydroxyl radical, and hydrogen peroxide; thus, the photodynamic generation of ROS may be the basis of phototoxicity of quinolones in human beings and animals. This...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22495
更新日期:2020-07-01 00:00:00
abstract::Cerium oxide nanoparticles (CeO2 NPs) are among the important nanoparticles that are extensively utilized in cosmetics, automotive industries, ultraviolet (UV) filtration, gas sensors, and pharmaceutical products. In this study, CeO2 NPs were synthesized using an aqueous extract of Ziziphus jujube fruit. The synthesiz...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22475
更新日期:2020-06-01 00:00:00
abstract::In this study, a series of novel bis-sulfone compounds (2a-2j) were synthesized by oxidation of the bis-sulfides under mild reaction conditions. The bis-sulfone derivatives were characterized by 1 H-NMR, 13 C-NMR, Fourier-transform infrared spectroscopy, and elemental analysis techniques. Nuclear Overhauser effect exp...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22401
更新日期:2019-11-01 00:00:00
abstract::Aluminum and thallium may reach life-threatening levels in aquatic systems in the near future because of their extensive use in various industrial fields. It is therefore important to study the mechanism of toxicity of aluminum and thallium on fish enzymes. To this aim, the effects of aluminum and thallium on the acti...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20200
更新日期:2007-01-01 00:00:00
abstract::The present study is the first attempt to report the characterization of a nucleotidase from Cerastes cerastes venom. A 70 kDa 5'-nucleotidase (Cc-5'NTase) was purified to homogeneity. The amino acid sequence of Cc-5'NTase displayed high homology with many nucleotidases. Its activity was optimal at pH 7 with a specifi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21885
更新日期:2017-05-01 00:00:00
abstract::Schisandrin A (SchA) has been reported as a multidrug resistance-reversing agent; however, its antitumor effects have been rarely reported. Consequently, we attempted to explore whether SchA per se possesses an antitumor property in choriocarcinoma JEG-3 and BeWo cells and its potential mechanisms. JEG-3, BeWo, and HT...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22455
更新日期:2020-04-01 00:00:00
abstract::Alkaline phosphatase (ALP) activity in the serum and liver from rats administered with cadmium (Cd) in drinking water was studied. After metal administration, Cd showed a time-dependent accumulation in the liver, meanwhile metallothionein had a maximum increase at 1 month, remaining in this level until the end of the ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21732
更新日期:2015-12-01 00:00:00
abstract::With the rapid development of nanotechnology, nanomaterials are now being used for cancer treatment. Although studies on the application of silver nanoparticles in cancer treatment are burgeoning, few studies have investigated the toxicology mechanisms of autophagy in cancer cells under exposure to sublethal silver na...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22474
更新日期:2020-05-01 00:00:00
abstract::Cisplatin is a widely used chemotherapeutic drug; however, it induces damage on kidney and liver at clinically effective higher doses. Morin hydrate possesses antioxidant, anti-inflammatory, and anticancer properties. Therefore, we aimed to investigate the effects of morin hydrate (50 and 100 mg/kg, orally) against th...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21817
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Cadmium is a nonessential toxic heavy metal, which enters the body easily and damages the cellular system. The sonic hedgehog (Shh) signaling pathway is one of the key regulatory pathways, which define neural growth and development. OBJECTIVES:This study aimed to explore how cadmium exposure affects neural ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22274
更新日期:2019-04-01 00:00:00
abstract::Uroporphyrinogen decarboxylase is an essential enzyme in all organisms and functions in the heme biosynthetic pathway, catalyzing the decarboxylation of the four acetate groups of uroporphyrinogen to form coproporphyrinogen. This work examines whether the four sequential decarboxylations occur at the same active site,...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20055
更新日期:2005-01-01 00:00:00
abstract::Paraoxon, the active metabolite of parathion, can be detoxified through a noncatalytic pathway by carboxylesterases and a catalytic pathway by calcium-dependent A-esterases, producing p-nitrophenol as a common metabolite. The detoxication patterns of carboxylesterases and A-esterases were investigated in vitro in the ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0461(1999)13:5<261::aid-jbt6>3.
更新日期:1999-01-01 00:00:00
abstract::The effect of 2-(2-nitrovinyl)furan on the redox status of male rat liver and kidney was evaluated. Twenty male rats were randomized into four groups; group A received olive oil and groups B, C, and D rats received 12.5, 25, and 50 mg/kg bodyweight of 2-(2-nitrovinyl)furan intraperitoneally, daily at 24 h interval, re...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21674
更新日期:2015-03-01 00:00:00