Abstract:
:The use of more lipophilic derivatives of 5-aminolevulinic acid (ALA) is expected to have better diffusing properties, and after conversion into the parent ALA, to reach a higher protoporphyrin IX (PPIX) formation rate, thus improving the efficacy of topical photodynamic therapy (PDT). Here we have analysed the behaviour of 3 ALA derivatives (ALA methyl-ester, hexyl ester and a 2-sided derivative) regarding PPIX formation, efficiency in photosensitizing cells and mechanism of cellular death. The maximum amount of porphyrins synthesized from 0.6 mM ALA was 47 +/- 8 ng/10(5)cells. The same amount was formed by a concentration 60-fold lower of hexyl-ALA and 2-fold higher of methyl-ALA. The 2-sided derivative failed to produce PPIX accumulation. Applying a 0.6 J cm(-2)light dose, cell viability decreased to 50%. With the 1.5 J cm(-2) light dose, less than 20% of the cells survive, and higher light doses produced nearly total cell killing. Comparing the PPIX production and the induced phototoxicity, the more the amount of porphyrins, the greater the cellular killing, and PPIX formed from either ALA or ALA-esters equally sensitize the cells to photoinactivation. ALA-PDT treated cells exhibited features of apoptosis, independently on the pro-photosensitizer employed. ALA-PDT can be improved with the use of ALA derivatives, reducing the amount of ALA necessary to induce efficient photosensitization.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Casas A,Fukuda H,Di Venosa G,Batlle Adoi
10.1054/bjoc.2001.1875subject
Has Abstractpub_date
2001-07-20 00:00:00pages
279-84issue
2eissn
0007-0920issn
1532-1827pii
S0007092001918750journal_volume
85pub_type
杂志文章abstract::The human gene sequences encoding the translation-associated functions of alpha-subunit of elongation factor 1 (EF-1 alpha) and the ubiquitin carboxyl extension protein (HUBCEP80) have been isolated by differential cDNA screening, and found to have significantly higher levels of expression in fibroadenomas (benign) co...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.12
更新日期:1992-01-01 00:00:00
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journal_title:British journal of cancer
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abstract::We investigated whether cholecystectomy is associated with subsequent cancer and, if so, whether the association is likely to be causal, by undertaking a retrospective cohort study using linked medical statistics, comprising a cholecystectomy group (n=39 254) and a reference cohort admitted for a range of other medica...
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2012-09-25 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1971.27
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doi:10.1038/bjc.1976.94
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doi:10.1038/bjc.1982.269
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abstract:BACKGROUND:Aspirin use has been shown to lower incidence and mortality in cancer patients. The aim of this population-based study was to determine the effect of postdiagnosis low-dose aspirin use on survival of patients with oesophageal cancer. METHODS:Patients with oesophageal cancer (1998-2010) were selected from th...
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.406
更新日期:1995-09-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1979.55
更新日期:1979-03-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.663
更新日期:1996-12-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2005-05-09 00:00:00
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