Abstract:
:Progressive renal dysfunction in 5/6 nephrectomized (NX) rats can be physiologically divided into three stages, coinciding with morphological stages, after definition of physiological parameters for identification of stage. Now, for the establishment of a toxicity screening approach using 5/6 NX rats, our concept, "Differential toxicity synchronized with renal dysfunction process could be identified using 5/6 NX rats" was examined by dosing gentamicin. Firstly, electrophoretic fractional changes of urinary proteins during gentamicin treatment were clarified with determination of amino acid sequences and the three differential features were proven, revealing the unpredictable depression of urinary albumin with progression of the stages in NX rats. Secondly, marked elevation of urinary lactate dehydrogenase (LDH) and glucose (GLU) was evident, indicating the intensified hypoxic conditions and glycolysis in tubular cells synchronized with increased tubular damage. Thirdly, these transit metabolic changes were proven as intensive cause for the advancement of renal dysfunction by the reduction of FRelectrolytes and water at the end of each dosing period. These results indicate that toxicity studies of newly developed drugs using 5/6 NX rats have potentiality prior to clinical dosing to the patients.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Barata K,Yoshida M,Hokao R,Imai S,Takahashi S,Harada E,Maekawa Adoi
10.1191/096032701666840048subject
Has Abstractpub_date
2001-02-01 00:00:00pages
100-10issue
2eissn
0960-3271issn
1477-0903journal_volume
20pub_type
杂志文章abstract::The objective of the present study was to perform histological and stereological examination of alcohol-induced changes in the optic nerve, considered an extension of white matter of the brain, in rats. This study included 20 male Wistar albino rats aged 60 days and weighing 190-220 g. The rats were divided into three...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119828123
更新日期:2019-05-01 00:00:00
abstract::1. A study was carried out to find out if digoxin and acipimox interact. 2. Six elderly patients on digoxin were each given acipimox 150 mg three daily for a week, after informed consent. Digoxin and acipimox plasma concentrations and urinary excretion were measured after the first dose of acipimox and after a week of...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719201100509
更新日期:1992-09-01 00:00:00
abstract::The polybrominated diphenyl ether flame retardants decabromodiphenyl ether (BDE-209) and bisphenol A (BPA) are environmental contaminants that can cross the placenta and exert toxicity in the developing fetal nervous system. Copy number variants (CNVs) play a role in a number of genetic disorders and may be implicated...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327117710535
更新日期:2018-05-01 00:00:00
abstract::The common effects of acetaminophen (paracetamol), isopropylantipyrine (propyphenazone) and caffeine on fetal development were examined in rats. The mixture was given in Tween 80 solution once daily, in a constant proportion of 5:3:1, during days 8-14 of gestation in three different doses. Dose S1 - 3.5 mg/kg acetamin...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327102ht224oa
更新日期:2002-01-01 00:00:00
abstract::Formaldehyde is a physiological intermediary metabolite taking part in many biological process in the body. It is a constituent of many items of daily use, including foods. It is also used in medicine for treatment of some conditions. A 40% solution of formaldehyde in water is known as formalin. Formalin is irritating...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,评审
doi:10.1191/096032700678815954
更新日期:2000-06-01 00:00:00
abstract:UNLABELLED:A single dose of monocrotaline, a pyrrolizidine alkaloid, was injected into rats in order to produce 25 (Group I) and 45 (Group II) days later a progressive and so called delayed liver injury. The present study investigated the prostanoid production of Kupffer cells and endothelial cells separated from Monoc...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719801701007
更新日期:1998-10-01 00:00:00
abstract::Sodium hypochlorite (NaClO) is used extensively as a disinfectant or bleaching agent. Most studies describe ingestion or inhalation route of this product with rare complication and fatalities. Despite global daily exposures, data about bleach injection is limited. Here we report intentional infusion of 20 mL, sodium h...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327111432506
更新日期:2012-06-01 00:00:00
abstract::The mechanism of doxorubicin (DOX)-induced cardiotoxicity remains controversial. Wistar rats (n = 66) received DOX injections intraperitoneally and were randomly assigned to 2 experimental protocols: (1) rats were killed before (-24 h, n = 8) and 24 h after (+24 h, n = 8) a single dose of DOX (4 mg/kg body weight) to ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327113512342
更新日期:2014-07-01 00:00:00
abstract::1. Polyvinyl chloride (PVC) cot mattress covers from SIDS cases were investigated as potential sources of soluble (potentially ingestable) antimony in the cot environment. 2. Body fluids (urine, saliva) and proprietary domestic detergents/sterilizing fluids markedly enhanced leaching of antimony from PVC. Release of a...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719801700302
更新日期:1998-03-01 00:00:00
abstract::The benefits and costs are discussed for the possibility of using pups generated in breeding studies for additional assessments, such as evaluating immunotoxicity. Such an approach is logistically challenging, but not overwhelming. ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327102ht291oa
更新日期:2002-09-01 00:00:00
abstract::4-hydroxynonenal (4-HNE) and 6-hydroxydopamine (6-OHDA)-mediated damage in dopaminergic neurons is well documented. Protective potential of steroidal hormone (17β-estradiol) has also been suggested. However, therapeutic potential of such promising hormone is hampered due to complex brain anatomy and physiology. Thus, ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110382130
更新日期:2011-08-01 00:00:00
abstract::Recent years have seen substantial shifts in cultural attitudes towards cannabis for medical and recreational use. However, legalizing recreational marijuana may have adverse effects on individual and public health. As the most widely used illicit agent, cannabis is commonly reported to disrupt learning and memory. Un...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119886037
更新日期:2020-03-01 00:00:00
abstract::The malignancy of non-small cell lung cancer (NSCLC) largely results from its invasive manner. Secreted frizzled-related proteins (SFRPs) have been recently found to suppress the invasiveness of some cancers. On the other hand, the methylation of SFRPs increases protein degradation to reduce the activity of SFRPs, res...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327117693071
更新日期:2018-02-01 00:00:00
abstract::1. Four different concentrations of quinalphos and methyl parathion were tested on human peripheral lymphocytes over different time periods, for the analysis of chromosomal aberrations and sister chromatid exchanges (SCEs). 2. A significant increase in chromosomal aberrations was observed when cells were treated with ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719000900605
更新日期:1990-11-01 00:00:00
abstract::Coronary heart disease and in particular its most serious form - acute myocardial infarction (AMI) - represents the most common cause of mortality in developed countries. Better prognosis may be achieved by understanding the etiopathogenetic mechanisms of AMI. Therefore, a catecholamine model of myocardial injury, whi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327109350665
更新日期:2009-10-01 00:00:00
abstract::Previous studies have shown that environmental exposure to heavy metals has been related to epigenetic changes, such as DNA methylation in receptors involved in pathogenesis of asthma. One of these receptors is beta-2 adrenergic receptor (ADRB2). We conducted this study to examine the association between blood aluminu...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120921436
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients' outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injurie...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120979014
更新日期:2020-12-16 00:00:00
abstract::In Southern Africa, the medicinal plant Warburgia salutaris is commonly used for the treatment of inflammatory and other diseases. The methanol extracts of W. salutaris were investigated with regard to a) production of proinflammatory cytokines tumor necrosis factor-alpha, interleukin-1beta, and interferon-gamma; b) a...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327108099536
更新日期:2008-11-01 00:00:00
abstract::Paraquat poisoning was studied in 174 patients over a 12-month period when a new, low concentration paraquat product (4.5% w/v paraquat ion mixed with 4.5% w/v diquat ion; 63 cases) replaced the original high concentration paraquat product (20% w/v paraquat ion only; 111 cases). In both groups approximately 60% of the...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719201100401
更新日期:1992-07-01 00:00:00
abstract::The effects of the environmental toxicant, triethyltin, on Ca2+ mobilization in Madin-Darby canine kidney (MDCK) cells have been examined. Triethyltin induced an increase in cytosolic free Ca2+ levels ([Ca2+]i) at concentrations larger than 2 microM in a concentration-dependent manner. Within 5 min, the [Ca2+]i signal...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327102ht276oa
更新日期:2002-08-01 00:00:00
abstract:AIM:Diazinon (DZN) is one of the most important organophosphorus compounds used to control pests in agriculture in many countries. Several studies have shown that exposure to DZN may alter protein expression in the liver. In order to further investigate the mechanism of DZN toxicity, differentially expressed ATP-intera...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327115619771
更新日期:2016-10-01 00:00:00
abstract::This report highlights a rare presentation of vitamin B(12) deficiency (concurrent psychotic disorder, seizures and hypertension). A 16-year-old girl presented with nervousness that had been persisting for 2 weeks. She had stopped eating and there was decreased self-care and she could not walk and sleep. Two days prio...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327111422404
更新日期:2012-04-01 00:00:00
abstract::Exposure of Co631 cells to plutonium leads to increasing cell death depending on the dose and incubation time. The effects, however, are more pronounced with 239Pu, than with 238Pu. Uptake and binding of the two radionuclides during a 5-h incubation are about equal, but 238Pu is lost more rapidly from the cell. There ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719201100303
更新日期:1992-05-01 00:00:00
abstract::Genotoxicants can be identified as aneugens and clastogens through a micronucleus (MN) assay. The current high-content screening-based MN assays usually discriminate an aneugen from a clastogen based on only one parameter, such as the MN size, intensity, or morphology, which yields low accuracies (70-84%) because each...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327117695635
更新日期:2018-03-01 00:00:00
abstract::Pesticides, including organophosphate (OP), organochlorine (OC), and carbamate (CB) compounds, are widely used in agricultural and indoor purposes. OP and CB act as acetyl cholinesterase (AChE) inhibitors that affect lots of organs such as peripheral and central nervous systems, muscles, liver, pancreas, and brain, wh...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,评审
doi:10.1177/0960327110388959
更新日期:2011-09-01 00:00:00
abstract::The effect of Glycyrrhiza glabra extract (GgE) as a natural antioxidant and melatonin (MEL) on ochratoxin A (OTA)-induced histopathological damages on the testes and oxidative stress was evaluated in male rats. The animals were assigned into four groups (n = 8) including control and test groups. The rats in control gr...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110368416
更新日期:2011-02-01 00:00:00
abstract::The pharmacokinetics of the herbicide fluazifop-butyl have been determined in female rats following oral and intravenous dosing, and described by a mathematical model. Penetration of fluazifop-butyl through epidermal membranes has been determined using three different receptor fluids. It is demonstrated how this in vi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200302
更新日期:1993-05-01 00:00:00
abstract::Acute exposure to systemic poisons represents an important challenge in clinical toxicology. We aimed to analyze the potential role of cardiac biomarkers, routine laboratory tests, and clinical scores as morbidity and in-hospital mortality predictors in patients intoxicated with various systemic poisons. We conducted ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327116660866
更新日期:2017-07-01 00:00:00
abstract::1. Carbofuran was administered orally to adult male rats at dose levels of 0.1, 0.2, 0.4 or 0.8 mg kg-1 body weight, 5 d wk-1 for 60 days. A dose dependent decrease was observed in body weight of rats treated with 0.2-0.8 mg carbofuran kg-1 body weight. 2. A significant decrease in the weight of epididymides, seminal ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719501401106
更新日期:1995-11-01 00:00:00
abstract::In this study, genotoxic activities of four halogenated anesthetics (halothane, isoflurane, sevoflurane and desflurane) were investigated in human peripheral blood lymphocytes (PBLs) and sperm cells in vitro by alkaline comet assay. For this purpose, sperm or lymphocyte suspension was exposed to different concentratio...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327112446818
更新日期:2012-12-01 00:00:00