Abstract:
:Inflammation elicits an acute phase response, which includes changes in plasma concentrations of a number of cytokines, reflecting changes in their gene transcription in the liver. In this study, the induction of complement factor 3 (C3) was investigated in HepG2 cells, a human hepatoma cell line often used as a model system for cytokine-dependent expression of acute phase proteins of the liver. By using a very sensitive RT-PCR assay, the amount of mRNA for C3 was measured after induction with lipopolysaccharide (LPS) and interleukin-6 (IL-6). Both substances were found to up-regulate C3 gene expression. C3 mRNA level was lower in LPS-treated cells compared to IL-6 induction and also reached maximum expression at an earlier time point. These findings suggest a coordinate stimulation of C3 expression in the hepatocytes, which then maintains the host response to infectious agents.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Wright MS,Sund NJ,Abrahamsen TGdoi
10.1016/s0165-2478(01)00180-8subject
Has Abstractpub_date
2001-03-01 00:00:00pages
119-23issue
2eissn
0165-2478issn
1879-0542pii
S0165247801001808journal_volume
76pub_type
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