Optimal expression of a Fab-effector fusion protein in Escherichia coli by removing the cysteine residues responsible for an interchain disulfide bond of a Fab molecule.

abstract：:When murine BALB/c splenocytes are pretreated for 2 h with different concentrations of 25-hydroxycholesterol (25-HC) or 7 beta-hydroxycholesterol (7-HC), washed and stimulated either with irradiated C57BL/6 splenocytes or with Con A, IL-2 secretion is inhibited in a dose-dependent way as well as the subsequent cell pr...

journal_title：Immunology letters

authors： Richert L,Beck JP,Shinitzky M

更新日期：1986-11-03 00:00:00

abstract：:Early diagnosis and immediately started appropriate treatment are mandatory for the prevention of radiographic progression, functional disability and unfavourable disease outcome in rheumatoid arthritis (RA). The current classification criteria for RA include two different types of biomarkers representing inflammatory...

journal_title：Immunology letters

authors： Nakken B,Papp G,Bosnes V,Zeher M,Nagy G,Szodoray P

更新日期：2017-09-01 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) is an attractive target for the treatment of epithelial cancers. However, EGFR antagonists have low clinical response rates and frequently induce resistance mainly caused by the hypermutation of the extracellular and intracellular domains and the heterodimerization of EGFR. Dime...

journal_title：Immunology letters

authors： Guo T,Zhao L,Zhang Y,Liu G,Yao Y,Li H

更新日期：2016-12-01 00:00:00

abstract：:H-2-linked gene(s) have been found to play a role in the quantitative regulation of response to human gamma-globulin (HGG) in mice selected for high or low antibody responsiveness to sheep erythrocytes. Unexpectedly, in a random genetically heterogeneous population of F2 interline hybrids, the gene(s) linked to the H-...

journal_title：Immunology letters

authors： Heumann AM,Mouton D,Biozzi G

更新日期：1982-11-01 00:00:00

abstract：:Administration of opioids that bind to the classical mu opioid receptor has been shown to lead to unintended alterations in immune function. Traditionally, altered immune function has been investigated with circulating immune cells. Effects of mu agonists on intestinal epithelial immune function have not been describe...

journal_title：Immunology letters

authors： Neudeck BL,Loeb JM

更新日期：2002-12-03 00:00:00

abstract：:Liposome-encapsulated conalbumin (L(conalbumin)) is an antigen that is efficiently phagocytosed by bone marrow-derived macrophages and presented to effector cells as part of the major histocompatibility complex (MHC) class I complex. In this report, we show that the conalbumin component of L(conalbumin) is degraded to...

journal_title：Immunology letters

authors： Rothwell SW,Wassef NM,Alving CR,Rao M

更新日期：2000-10-03 00:00:00

abstract：:This study aimed to investigate the influence of physical activity in innate immunity to conduce to an effective antitumoral immune response analyzing the phenotype and activation status of infiltrating cells. We analysed the intracellular cytokines and the transcription factors of tumor infiltrating lymphocytes (TILS...

journal_title：Immunology letters

authors： Bianco TM,Abdalla DR,Desidério CS,Thys S,Simoens C,Bogers JP,Murta EFC,Michelin MA

更新日期：2017-10-01 00:00:00

abstract：:Work done in our laboratories, using a murine model, indicates that suppression of host immune responses might be due to secretion of soluble factors by tumor cells. The H238 cells (BALB/c embryonic fibroblasts transformed by UV-inactivated herpes simplex virus Type 2) exhibit progressive tumor growth with subsequent ...

journal_title：Immunology letters

authors： Das MR,Gridley DS,Kettering JD

更新日期：1991-09-01 00:00:00

abstract：:Experiments with malaria in mice suggest that protective immunity depends not only on antibody but also on activation of macrophages. Activated macrophages may cause intra-erythrocytic death of parasites by releasing reactive oxygen intermediates and/or tumour necrosis factor. Macrophage activation for both types of p...

journal_title：Immunology letters

authors： Playfair JH,Dockrell H,Taverne J

更新日期：1985-01-01 00:00:00

abstract：:Recombinant gp120 of the HIV-1(IIIB) isolate (BH10 clone) has been mutated to form the PR12 protein with the first 74 C-terminal amino acids and the V1, V2 and V3 hypervariable loops deleted. A variety of studies have shown that the CD4 binding domain (CD4bd) is very well exposed in PR12 in contrast to rgp120(LAI). Us...

journal_title：Immunology letters

authors： Jeffs SA,Gorny MK,Williams C,Revesz K,Volsky B,Burda S,Wang XH,Bandres J,Zolla-Pazner S,Holmes H

更新日期：2001-12-03 00:00:00

abstract：:Multiple antigen peptides (MAP), consisting of a number of peptide copies synthesized on a branching lysyl core, offer a novel approach for rendering small peptides immunoreactive in solid-phase immunoassays. An octameric MAP, carrying 6 repeats of the sequence -N-A-A-G-, tandem repeated in the immunodominant region o...

journal_title：Immunology letters

authors： Habluetzel A,Pessi A,Bianchi E,Rotigliano G,Esposito F

更新日期：1991-09-01 00:00:00

abstract：:There is great interest in the antibodies-to-DNA transformation, since this change is characteristic of autoimmune diseases and contributes to its pathology. After immunization and fusions, 14 hybridomas bearing DNA-hydrolysis activity against pUC19 plasmid DNA were obtained. Genes coding for V(H) and V(L) regions of ...

journal_title：Immunology letters

authors： Zein HS,Teixeira da Silva JA,Miyatake K

更新日期：2010-03-10 00:00:00

abstract：:We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...

journal_title：Immunology letters

authors： Blank M,Smorodinsky IN,Keydar I,Chaitchik S,Shoenfeld Y

更新日期：1991-04-01 00:00:00

abstract：:Interleukin 18 (IL-18) gene polymorphisms have been reported to be associated with the outcomes of hepatitis C virus (HCV) infection in Americans, Indians and Europeans. We aimed to investigate whether the association can be replicated in Chinese Han population. Three IL-18 variants, -656G>T, -137G>C and +105A>C, were...

journal_title：Immunology letters

authors： Yue M,Wang JJ,Tang SD,Feng L,Zhang Y,Liu Y,Wang J,Deng XZ,Xu K,Zhang J

更新日期：2013-07-01 00:00:00

abstract：:Four murine monoclonal antibodies (mAbs) showing similar reactivity with a cell-wall extract and mannan preparation obtained from Candida albicans were examined for epitope specificity. An enzyme-linked immunosorbent assay (ELISA) was used to determine total mAb binding to extracted cell-wall antigen when each mAb was...

journal_title：Immunology letters

authors： Chaffin WL,Morrow KJ Jr

更新日期：1989-07-01 00:00:00

abstract：:When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4delta2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of...

journal_title：Immunology letters

authors： Chang JS,Dheda K,Huggett JF,Kim LU,Zumla A,Rook GA

更新日期：2006-08-15 00:00:00

abstract：:A fragment of non-glycosylated E2 antigen of classical swine fever virus (CSFV), lacking the trans-membrane anchor (TM-) of the native glycoprotein, was produced in recombinant Escherichia coli strain BL21(DE3) in the form of inclusion bodies. These inclusion bodies isolated from the bacteria cells were administrated ...

journal_title：Immunology letters

authors： Kesik M,Saczyńska V,Szewczyk B,Płucienniczak A

更新日期：2004-02-15 00:00:00

abstract：:The spontaneous migration in vitro, production of T cell migration inhibitory factor (TIF) and the response to TIF of OKT4+ and OKT8+ human T cell subsets were studied. The OKT4+ lymphocytes migrated far better than the OKT8+ cells although the movement of both subsets was comparably inhibited by TIF. The OKT8+ subset...

journal_title：Immunology letters

authors： Kowalczyk D,Pryjma J,Zembala M

更新日期：1986-08-01 00:00:00

abstract：:Disease-modifying osteoarthritis (OA) therapy is not yet available. Several adjuvant therapies have demonstrated promising results in the treatment of OA. The present study aimed to investigate the therapeutic effects and underlying mechanisms of a combination of Lactobacillus acidophilus, vitamin B, and curcumin in t...

journal_title：Immunology letters

authors： Jhun J,Min HK,Na HS,Kwon JY,Ryu J,Cho KH,Choi J,Jung K,Lee SY,Kim SJ,Yang CW,Park SH,Cho ML

更新日期：2020-12-01 00:00:00

abstract：:We have utilized a limiting dilution assay, involving a minimum of in vitro culture, to determine the frequencies of CD4+ T cells which have the capacity to secrete IL-2 (Th1), IL-4 (Th2) or IL-2 and IL-4 (Th0). CD4+ lymph node cells obtained from unimmunized mice were found to contain 40% Th1, 34% Th2, and 26% Th0 ce...

journal_title：Immunology letters

authors： Torbett BE,Laxer JA,Glasebrook AL

更新日期：1990-01-01 00:00:00

abstract：:A survey of the appearance of natural antibodies in C57BL/6 mice, measured by a complement-dependent cytotoxicity test against syngeneic radiation leukemia virus-induced lymphoma, was performed. Activity could be detected in sera from 5- to 14-month-old animals. This activity could be attributed mainly to IgM antibodi...

journal_title：Immunology letters

authors： Rubio N

更新日期：1981-10-01 00:00:00

abstract：:We have examined the effect of different monoclonal antibodies (MAb) to the CD11/CD18 family of surface antigens on the production of superoxide anion by phorbol myristate acetate (PMA)-differentiated U-937 cells upon stimulation with concanavalin A (Con A). The anti-CD11c MAb HC1/1 displayed a significant inhibition,...

journal_title：Immunology letters

authors： Cabañas C,Lacal P,Mollinedo F,López-Rivas A,Sánchez-Madrid F,Bernabeu C

更新日期：1989-02-01 00:00:00

abstract：:The effect of priming with various antigens on subsequent vaccination with the pneumococcal conjugate vaccine (CPV) was determined using BALB/c mice. Priming with pneumococcal polysaccharide or cross-reactive polysaccharide did not inhibit the IgG response to CPV immunization. Additionally, live intranasal colonizatio...

journal_title：Immunology letters

authors： Rabquer B,Smithson SL,Shriner A,Julie Westerink MA

更新日期：2006-08-15 00:00:00

abstract：:An autoreactive T lymphocyte clone, designated as F1C4 was established from an autoimmune mouse strain (NZB/NZW)F1. This clone proliferated in the presence of mitomycin C-treated splenic adherent cells (MMC-SAC) from syngeneic mice. This response was dependent on the numbers of MMC-SAC. The specificity of F1C4 for I-A...

journal_title：Immunology letters

authors： Naiki M,Imai Y,Kamita Y,Osawa T

更新日期：1988-08-01 00:00:00

abstract：:Administration of cyclosporin A (CS-A; 25 mg/kg daily) to rats from the time of immunization with ovalbumin (OVA) in complete Freund's adjuvant abolished the production of anti-OVA antibodies, including IgE. Cyclophosphamide (Cy; 150 mg/kg) given 2 days before immunization also inhibited specific antibody production b...

journal_title：Immunology letters

authors： Thomson AW,Brown PA,Simpson JG,Whiting PH,Davidson RJ

更新日期：1985-01-01 00:00:00

abstract：:BAFF deficiency in mice impairs B cell development beyond the transitional stage 1 in the spleen and thus severely reduces the size of follicular and marginal zone B cell compartments. Moreover, humoral immune responses in these mice are dramatically impaired. We now addressed the question whether the decrease in matu...

journal_title：Immunology letters

authors： Swee LK,Tardivel A,Schneider P,Rolink A

更新日期：2010-06-15 00:00:00

abstract：:Even though IgA is considered to play an important role in immunity, surprisingly little is known about the presence of IgA Fc receptor (Fc alpha R)-expressing effector cells in tissues. Difficulties in obtaining human tissue macrophages, led us to study peritoneal macrophages in a human Fc alpha RI transgenic (Tg) mo...

journal_title：Immunology letters

authors： van Egmond M,Hanneke van Vuuren AJ,van de Winkel JG

更新日期：1999-05-03 00:00:00

abstract：:Two cell lines producing monoclonal antibodies have been established from peripheral blood of a negative Rhesus blood donor which has been immunized with positive Rhesus red blood cells. Two monoclonal antibodies Co II 8.8 and Co II 7.12 have been selected. Both are IgG1 antibodies, but recognize different epitopes on...

journal_title：Immunology letters

authors： Paire J,Monestier M,Rigal D,Martel F,Desgranges C

更新日期：1986-09-01 00:00:00

abstract：:Many details in the expression pattern of Epstein-Barr virus (EBV)-encoded proteins, their role in blast and growth transformation in infected B lymphocytes are known, but 'black holes' still exist. The two main types of virus-B lymphocyte interactions are denoted as Type I and Type III. These are characterized by the...

journal_title：Immunology letters

authors： Klein E,Teramoto N,Gogolák P,Nagy N,Björkholm M

更新日期：1999-05-03 00:00:00

abstract：:Cellular membranes contain microdomains known as 'lipid rafts' or detergent-insoluble microdomains (DRM), enriched in cholesterol and sphingolipids. DRM can play an important role in many cellular processes, including signal transduction, cytoskeletal organization, and pathogen entry. Many receptors like T cell recept...

journal_title：Immunology letters

authors： Beekman JM,van der Linden JA,van de Winkel JG,Leusen JH

更新日期：2008-03-15 00:00:00