Abstract:
PURPOSE:In keratoconus corneas, it has been shown that levels of degradative enzymes and transcription factor Sp1 are elevated and those of inhibitors are reduced, especially in the epithelial layer. This study is to determine whether the biochemical abnormalities identified in corneas also exist in conjunctival tissues. METHODS:Conjunctival tissues were collected from normal subjects and from patients with keratoconus, senile cataract, and other corneal diseases. Immunohistochemical staining for cathepsins B and G, alpha 1-proteinase inhibitor, alpha 2-macro-globulin and Sp1 was performed. RESULTS:The epithelium of all conjunctival specimens showed immunoreactivity toward the antibodies. The staining for cathepsins B and G, and the inhibitors was mostly cytoplasmic, while that for Sp1 was nuclear. The staining intensity in keratoconus specimens was all within normal range. CONCLUSIONS:These results suggest that the abnormalities in cathepsins B and G, protease inhibitors and Sp1 identified in keratoconus corneas are not manifested in the conjunctiva. Keratoconus appears to be a disease localized to the cornea.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Maruyama I,Zhou L,Sugar J,Yue BYdoi
10.1076/ceyr.21.5.886.5538subject
Has Abstractpub_date
2000-11-01 00:00:00pages
886-90issue
5eissn
0271-3683issn
1460-2202journal_volume
21pub_type
杂志文章abstract:PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1250278
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Form-deprivation myopia (FDM) is believed to result principally from actions of substances that modulate information processing in the retina. We used a chick model to investigate what role nitric oxide (NO), a gaseous neuromodulator, might play in the development of FDM. METHODS:We injected different concentr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.10.992.9021
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:To determine the plasma levels of the sulfur-containing amino-acids homocysteine, cysteine, cysteinylglycine, glutamylcysteine, glutathione, and taurine in patients with central retinal vein occlusion (CRVO) and in healthy subjects and to ascertain whether there are statistically significant differences between...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003698863
更新日期:2010-07-01 00:00:00
abstract::Activity of RNA polymerases I, II and III (distinguished using alpha-amanitin) and activity of DNA polymerases alpha and beta (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carba...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709025204
更新日期:1987-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500246957
更新日期:2005-11-01 00:00:00
abstract:PURPOSE:Taurolidine is a broad-spectrum, non antibiotic antimicrobial agent, not previously tested against the common causes of bacterial keratitis. This study, employing an experimental rabbit model of Staphylococcus aureus keratitis, investigated the effectiveness of topical taurolidine in reducing the number of bact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490908733
更新日期:2005-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1789664
更新日期:2021-02-01 00:00:00
abstract::Goggles that degrade the retinal image produce axial enlargement of the ocular globe and large myopic refractive errors. Many authors have assumed that visual image degradation itself leads to myopia. Hodos and co-authors have shown, however, that goggled eyes in chicks are considerably warmer than normal. Such temper...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999467
更新日期:1993-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1484144
更新日期:2018-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1522649
更新日期:2019-02-01 00:00:00
abstract::We studied the protective effects of passive immunization with virus specific antibody in newborn rabbits inoculated subcutaneously with type 2 herpes simplex virus (HSV-2). Newborn rabbits given anti-HSV-2 antibody intraperitoneally (IP) on days 0, 2 and 4 post infection had smaller herpetic skin lesions and reduced ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709020084
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.669005
更新日期:2012-06-01 00:00:00
abstract::Four groups of BALB/c mice were treated locally with different concentrations (0.1-1.0%) of retinoic acid (Etretinate) ointment for a period of one to four weeks. A fifth group was treated with the ointment base only. Ia-positive dendritic cells were identified by an indirect immunofluorescence technique which was con...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689509033511
更新日期:1995-03-01 00:00:00
abstract::Phosphatidic acid (PA) is an obligate intermediate in the phosphatidylinositol (PI) cycle and may serve as a sensitive marker for this pathway of reactions. Previously, we have shown that acetylcholine (ACh) stimulates formation of labeled PA in whole rat corneas whose phospholipids were prelabeled with [14C]arachidon...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034832
更新日期:1987-05-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells ar...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001869
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To assess changes in retinal vascular caliber in response to short-term use of nepafenac eye drops in patients with mild diabetic macular edema. MATERIALS AND METHODS:Thirty-four patients with previously untreated bilateral mild diabetic macular edema were included in this prospective study. For each participa...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2017.1399425
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:To characterize the pupil light reflex (PLR), electroretinographic (ERG) and tonometric parameters in healthy rat eyes. METHODS:Brown Norway rats were used for experiments. The PLR was evaluated with a computerized pupillometer (n = 27), ERGs were recorded simultaneously from both eyes (n = 27) and IOP was mea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.2.69.10156
更新日期:2002-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.1.1.5422
更新日期:2001-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1540704
更新日期:2019-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000822
更新日期:1984-10-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.2.132.5610
更新日期:1998-02-01 00:00:00
abstract::Recently, all the structural features of non-proliferative diabetic retinopathy have been demonstrated in mice fed 30% galactose for 21-26 months. To determine whether changes in retinal matrix protein mRNA levels occur early in the course of murine diabetes we used a competitive RT-PCR method to quantitate retinal mR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract:PURPOSE:To identify vitreous proteins that were differentially expressed in patients suffering from proliferative diabetic retinopathy with active neovascularization. METHODS:The vitreous samples of 15 active proliferative diabetic retinopathy patients were analyzed by two-dimensional gel electrophoresis and mass spec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600557030
更新日期:2006-03-01 00:00:00
abstract:PURPOSE:To determine the role of PKCγ in the regulation of gap junction coupling in the normal lens, we have compared the properties of coupling in lenses from wild type (WT) and PKC-γ knockout (KO) mice. METHODS:Western blotting, confocal immunofluorescence microscopy, immunoprecipitation, RT-PCR and quantitative rea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.573899
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:To evaluate the toxicity of indocyanine green (ICG) in combination with light. METHODS:Human retinal pigment epithelial cells (ARPE-19) and rat neurosensory retinal cells (R28) were treated with four different concentrations of ICG in combination with light exposure. Cell viability, mitochondrial function, and...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590959312
更新日期:2005-06-01 00:00:00
abstract::A model system for retinal adhesion, consisting of interacting bovine pigment, epithelium (PE) cells and photoreceptor outer segments, was utilized to examine any adhesive role of the interphotoreceptor matrix. PE cells were dissociated by trypsin treatment of the eyecup, and were allowed to replenish their surface pr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001859
更新日期:1981-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.542867
更新日期:2011-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1420198
更新日期:2018-04-01 00:00:00
abstract::Two alternative methods for the study of the lens cytoskeleton are described which serve to overcome some of the difficulties imparted by the unique biology of the lens. The first technique involves rapid freezing, thick sectioning, and selective extraction and/or fixation of the lens section. This approach offers sev...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997582
更新日期:1990-11-01 00:00:00