Diagnosis and monitoring of hepatic injury. II. Recommendations for use of laboratory tests in screening, diagnosis, and monitoring.

abstract：:Extensive data implicate cholesterol in the atherosclerotic process responsible for coronary disease. Of the atherosclerotic disease outcomes, serum cholesterol is most strongly related to coronary disease. A significant relationship of serum cholesterol to all clinical manifestations of coronary heart disease has bee...

journal_title：Clinical chemistry

authors： Kannel WB

更新日期：1988-01-01 00:00:00

abstract：BACKGROUND:Monogenic autoinflammatory diseases are caused by pathogenic variants in genes that regulate innate immune responses, and are characterized by sterile systemic inflammatory episodes. Since symptoms can overlap within this rapidly expanding disease category, accurate genetic diagnosis is of the utmost importa...

journal_title：Clinical chemistry

authors： Shinar Y,Ceccherini I,Rowczenio D,Aksentijevich I,Arostegui J,Ben-Chétrit E,Boursier G,Gattorno M,Hayrapetyan H,Ida H,Kanazawa N,Lachmann HJ,Mensa-Vilaro A,Nishikomori R,Oberkanins C,Obici L,Ohara O,Ozen S,Sarkisian T

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:We explored the utility of multiplexed temperature gradient capillary electrophoresis (TGCE) as a screening tool for identifying genetic changes in the human mitochondrial genome. We examined changes in mitochondrial DNA (mtDNA) in nonmelanoma skin cancers (NMSCs), using TGCE to resolve genetic differences c...

journal_title：Clinical chemistry

authors： Girald-Rosa W,Vleugels RA,Musiek AC,Sligh JE

更新日期：2005-02-01 00:00:00

abstract：:Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nea...

journal_title：Clinical chemistry

authors： Acton WJ,Van Duyn OM,Allen LV,Flournoy DJ

更新日期：1982-01-01 00:00:00

abstract：:The isoenzyme pattern of N-acetyl-beta-glucosaminidase (NAG) in serum and urine was studied in two groups of patients with diabetes mellitus and in 30 control subjects. Total NAG activity was significantly (P less than 0.001) increased in the serum and urine of the 20 diabetics with vascular complications, but was ins...

journal_title：Clinical chemistry

authors： Severini G,Aliberti LM,Di Girolamo M

更新日期：1988-12-01 00:00:00

abstract：BACKGROUND:An accelerated stability model based on the Arrhenius Equation can be used to estimate stability of diagnostic reagents. Here we review 3 examples in which the model does not accurately predict the stability of diagnostic reagents. METHODS:We prepared several pilot lots of quality controls materials contain...

journal_title：Clinical chemistry

authors： Ebrahim A,DeVore K,Fischer T

更新日期：2020-12-04 00:00:00

abstract：:Autocorrelation of clinical chemistry quality-control (Q/C) measurements causes one of the basic assumptions underlying the use of Levey-Jennings control charts to be violated and performance to be degraded. This is the requirement that the observations be statistically independent. We present a proposal for a new app...

journal_title：Clinical chemistry

authors： Alwan LC,Bissell MG

更新日期：1988-07-01 00:00:00

abstract：:A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without...

journal_title：Clinical chemistry

authors： Ohkubo A,Kamei S,Yamanaka M,Arai F,Kitajima M,Kondo A

更新日期：1981-07-01 00:00:00

abstract：:We assayed norepinephrine and epinephrine by utilizing solvent extraction, fluorescence derivatization, and "high-performance" liquid chromatography. A 100-microL aliquot of urine was extracted twice according to Smedes et al. (J Chromatogr 1982;231:25-39) and subsequently incubated with 1,2-diphenylethylenediamine. A...

journal_title：Clinical chemistry

authors： Moleman P,van Dijk J

更新日期：1990-05-01 00:00:00

abstract：:We describe a method for determination of guanidino compounds in plasma and erythrocytes. The compounds are separated by liquid chromatography, the concentrations in the effluent being estimated fluorometrically (Trans. Am. Soc. Artif. Intern. Organs 24: 61, 1978). Guanidinoacetic acid, guanidinosuccinic acid, guanidi...

journal_title：Clinical chemistry

authors： Kikuchi T,Orita Y,Ando A,Mikami H,Fujii M,Okada A,Abe H

更新日期：1981-11-01 00:00:00

abstract：BACKGROUND:Hyperglycosylated human chorionic gonadotropin (hCG-h) contains larger and more complex carbohydrate chains than regular human chorionic gonadotropin (hCG). hCG-h is thought to be the major form of hCG produced by testicular cancers and it has been suggested to play a key role in tumor invasion, but studies ...

journal_title：Clinical chemistry

authors： Lempiäinen A,Hotakainen K,Blomqvist C,Alfthan H,Stenman UH

更新日期：2012-07-01 00:00:00

abstract：:Plasma concentrations of delta-9-tetrahydrocannabinol, the principal psychoactive cannabinoid in marijuana, decline to values substantially less than 1 microgram/L within a few hours after a subject has smoked a marijuana cigarette. Using a single-quadrupole gas chromatograph-mass spectrometer (GC/MS) operated in the ...

journal_title：Clinical chemistry

authors： Shaw LM,Edling-Owens J,Mattes R

更新日期：1991-12-01 00:00:00

abstract：BACKGROUND:The liver is frequently subject to insult because of viral infection, alcohol abuse, or toxic chemical exposure. Extensive research has been conducted to identify blood markers that can better discern liver damage, but little progress has been achieved in clinical practice. Recently, circulating microRNAs (m...

journal_title：Clinical chemistry

authors： Zhang Y,Jia Y,Zheng R,Guo Y,Wang Y,Guo H,Fei M,Sun S

更新日期：2010-12-01 00:00:00

abstract：:We describe the case of a three-year-old girl with Wilms' tumor, whose serum showed at least a fivefold increase in relative viscosity although concentrations of albumin and immunoglobulins were normal. An unusual electrophoretogram of serum protein prompted further investigation. The increased viscosity was caused by...

journal_title：Clinical chemistry

authors： Wu AH,Parker OS,Ford L

更新日期：1984-06-01 00:00:00

abstract：BACKGROUND:Age-adjusted morbidity and mortality rates from coronary heart disease (CHD) are higher in men than in women. Androgens are suspected to be responsible for the male disadvantage. The genomic effect of androgens is mediated by the androgen receptor (AR), which has a polymorphic CAG repeat in exon 1. The numbe...

journal_title：Clinical chemistry

authors： Hersberger M,Muntwyler J,Funke H,Marti-Jaun J,Schulte H,Assmann G,Lüscher TF,von Eckardstein A

更新日期：2005-07-01 00:00:00

abstract：BACKGROUND:The "unit" for "enzymic activity" (U = 1 micromol/min) was recommended by the International Union of Biochemistry and Molecular Biology (IUB) in 1961 and is widely used in medical laboratory reports. The general trend in metrology, however, is toward global standardization through defining units coherent wit...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：2002-03-01 00:00:00

abstract：:Monoclonal antibody CKM-G01 inhibited greater than 99% of the activity of porcine and human creatine kinase(CK)-MM isoenzyme purified from muscle. However, it inhibited only 54% of CK-MM in human serum. Chromatofocusing of serum CK-MM showed that CKM-G01 inhibited 100% of MM3 but not isoform MM1. CKM-G01 inhibited CK-...

journal_title：Clinical chemistry

authors： Suzuki T,Shiraishi T,Tomita K,Totani M,Murachi T

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Rapid intraoperative parathyroid hormone (PTH) measurements are an important prerequisite for minimally invasive parathyroidectomy, serving as a feasible marker for "cure" because of the short half-life of PTH. Because automated analysis may facilitate monitoring, two automated PTH assays were compared with ...

journal_title：Clinical chemistry

authors： Bieglmayer C,Prager G,Niederle B

更新日期：2002-10-01 00:00:00

abstract：BACKGROUND:The aims of this study were to measure the N-terminal fragment of pro-brain natriuretic peptide (proBNP) in plasma in medical conditions commonly found in primary care and to evaluate the utility of these measurements in identifying impaired cardiac function in elderly patients with symptoms associated with ...

journal_title：Clinical chemistry

authors： Alehagen U,Lindstedt G,Eriksson H,Dahlström U

更新日期：2003-08-01 00:00:00

abstract：:The currently available radioimmunoassays of trypsin measure total immunoreactive trypsin (EC 3.1.1.7), which includes both trypsinogen and alpha 1-protease inhibitor-bound trypsin. Hitherto, the only way to differentiate these two forms of trypsin has been to fractionate them on a gel-filtration column. We describe h...

journal_title：Clinical chemistry

authors： Fletcher TS,Tsukamoto H,Largman C

更新日期：1986-09-01 00:00:00

abstract：BACKGROUND:Low concentrations of cardiac troponin (cTn) have been recommended for rapid rule-out of acute myocardial infarction (AMI). We examined the Beckman Coulter Access high-sensitivity cardiac troponin I (hs-cTnI) assay to identify a single test threshold that can safely rule out AMI. METHODS:This analysis used ...

journal_title：Clinical chemistry

authors： Greenslade J,Cho E,Van Hise C,Hawkins T,Parsonage W,Ungerer J,Tate J,Pretorius C,Than M,Cullen L

更新日期：2018-05-01 00:00:00

abstract：:The kidney and its response to the antidiuretic hormone (ADH) are the principal protective mechanisms necessary to maintain a normal plasma tonicity (osmolality). Hence, determination of the response of the ADH-renal axis to an abnormal plasma tonicity is an important step to understanding water homeostasis. Determina...

journal_title：Clinical chemistry

authors： Shoker AS

更新日期：1994-07-01 00:00:00

abstract：BACKGROUND:Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and diagnosis of MPS II. Our...

journal_title：Clinical chemistry

authors： Dean CJ,Bockmann MR,Hopwood JJ,Brooks DA,Meikle PJ

更新日期：2006-04-01 00:00:00

abstract：:We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this me...

journal_title：Clinical chemistry

authors： Shihabi ZK,Oles KS

更新日期：1994-10-01 00:00:00

abstract：BACKGROUND:A sensitive gas chromatography-negative ion chemical ionization mass spectrometry (GC/MS-NICI) method was developed and validated for the measurement of Delta(9)-tetrahydrocannabinol (THC) in human sweat patches. METHODS:THC-d(0) and THC-d(3) were added to worn blank sweat patches (PharmChek; PharmChem Inco...

journal_title：Clinical chemistry

authors： Saito T,Wtsadik A,Scheidweiler KB,Fortner N,Takeichi S,Huestis MA

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：:We describe a new multilayer immunoassay element for the determination of haptens in undiluted serum and plasma. Polysaccharide layers are coated onto a plastic base. The signal layer contains an immobilized antibody and a fluorescent-labeled hapten. A second layer, containing a pigment, acts as an optical screen. Sam...

journal_title：Clinical chemistry

authors： Grenner G,Inbar S,Meneghini FA,Long EW,Yamartino EJ Jr,Bowen MS,Blackwood JJ,Padilla AJ,Maretsky D,Staedter M

更新日期：1989-09-01 00:00:00

abstract：:We describe the use of electron-capture gas chromatography or reversed-phase "high-performance" liquid chromatography for concurrent analysis for diazepam and nordiazepam in serum. In the gas-chromatographic analysis our use of a new chemically deactivated stationary liquid phase, SP 2250-DB (Supelco, Inc.), resulted ...

journal_title：Clinical chemistry

authors： Wallace JE,Schwertner HA,Shimek EL Jr

更新日期：1979-07-01 00:00:00

abstract：BACKGROUND:Lead is an environmental pollutant, and human exposure is assessed by monitoring lead concentrations in blood. Because the main source of environmental exposure has been the use of leaded gasoline, its phase-out has led to decreased lead concentrations in the general population. Therefore, validated analytic...

journal_title：Clinical chemistry

authors： Patriarca M,Castelli M,Corsetti F,Menditto A

更新日期：2004-08-01 00:00:00

abstract：:We describe a solid-phase radioimmunometric sandwich assay for a new tumor marker defined by a monoclonal antibody (19-9). This antibody reacts with a carbohydrate antigenic determinant (CA 19-9) found at low concentrations in sera from healthy individuals but frequently increased in sera from patients with adenocarci...

journal_title：Clinical chemistry

authors： Del Villano BC,Brennan S,Brock P,Bucher C,Liu V,McClure M,Rake B,Space S,Westrick B,Schoemaker H,Zurawski VR Jr

更新日期：1983-03-01 00:00:00