Abstract:
:A deletional analysis of the SP-A1 promoter in NCI-H441 cells was performed to identify potential cis-acting elements involved in phorbol ester-mediated repression of human SP-A transcription. The phorbol ester TPA reduced SP-A1 and SP-A2 promoter activity to approximately 35% to 45% compared to that of control cells. The inhibitory effect of TPA was significantly reduced upon removal of the region +64/+394 relative to the SP-A1 transcription start site. Using NCI-H441 nuclear proteins, electromobility shift assay analysis showed that the intron region +309/+329 of SP-A1 and the corresponding region of SP-A2 formed sequence-specific DNA/protein complexes that were induced by TPA exposure. The region +318/+324 of SP-A1 contains sequences similar to a consensus AP-1 binding site, TGACTGA (TCACTGA for SP-A2), which when mutated (TGAGAGT) prevented the formation of the TPA-induced DNA/protein complex. The TPA-induced complex was supershifted in the presence of antibody against the Jun family of proteins, but not the Fos family of proteins. These results suggest that the binding of AP-1 or an AP-1--like factor to the first intron of SP-A1 and SP-A2 may be involved in the phorbol ester inhibition of human SP-A gene expression.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Hoover RR,Pavlovic J,Floros Jdoi
10.1080/019021400408272subject
Has Abstractpub_date
2000-07-01 00:00:00pages
303-17issue
5eissn
0190-2148issn
1521-0499journal_volume
26pub_type
杂志文章abstract::Diesel exhaust particles (DEP) are associated with respiratory disease and exposure to diesel exhaust induces an inflammatory response associated with marked leukocytic infiltration in the lung. This study examined whether neutrophils are activated by the active component of DEP (methanol extract of DEP [me-DEP]). The...
journal_title:Experimental lung research
pub_type: 杂志文章
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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abstract::The Ca(2+)-mobilizing actions of adenosine 5'-triphosphate (ATP), bradykinin, and histamine were compared in phenotypically distinct human nasal epithelial (HNE) cell types and as a function of time in cell culture. Single-cell measurements of intracellular free Ca2+ (Ca2+i, Fura-2 fluorescence) were recorded in cilia...
journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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更新日期:2011-02-01 00:00:00
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doi:10.3109/01902148.2013.860568
更新日期:2014-03-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148609064295
更新日期:1986-01-01 00:00:00
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journal_title:Experimental lung research
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doi:10.1080/019021401750069366
更新日期:2001-03-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2013.816803
更新日期:2013-10-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061513
更新日期:1983-11-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148.2015.1054530
更新日期:2015-01-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209069657
更新日期:1982-11-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609046037
更新日期:1996-07-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148009065463
更新日期:1980-08-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148809064185
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doi:10.3109/01902148.2014.900156
更新日期:2014-04-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1080/01902140701756778
更新日期:2007-12-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2009-12-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2020-11-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2000-10-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148709064313
更新日期:1987-01-01 00:00:00
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更新日期:2012-05-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148209039246
更新日期:1982-12-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2002-07-01 00:00:00