Abstract：
:Rats were given unilateral 6-hydroxydopamine (6-OHDA) lesions and subsequently received transplants of fetal ventral mesencephalic tissue into the denervated striatum. Four weeks later transplanted animals were tested for graft-mediated reduction of amphetamine-induced rotational behavior. Subsequently, transplanted animals received an intrastriatal injection of either GDNF (10 microg) or citrate buffer into a site lateral to the transplant, and then 6 h later received an injection of either 4.0 microg of 6-OHDA, 8.0 microg of 6-OHDA, or vehicle using the same stereotaxic coordinates that were used for the GDNF/citrate buffer injection. Animals were re-tested for amphetamine-induced rotational behavior 2 weeks later. Histological analysis revealed a significant reduction in the number of cell bodies immunostained for tyrosine hydroxylase (TH+) within the transplant for those animals pretreated with an intrastriatal injection of citrate buffer and subsequently given either dose of 6-OHDA. Transplanted animals pretreated with GDNF and subsequently administered 8.0 microg of 6-OHDA showed a significant reduction of TH+ neurons within the transplant compared to controls, however TH+ cell counts for this group remained significantly higher than the TH+ cell counts for the group of animals receiving the same dose of 6-OHDA but pretreated with citrate buffer. GDNF pretreatment completely protected TH+ cell bodies against 4.0 microg of 6-OHDA. Rotational scores indicated that GDNF provided only partial protection against 6-OHDA neurotoxicity in terms of transplant function. For both groups of transplanted animals receiving GDNF pretreatment and 6-OHDA injections, amphetamine-induced rotational scores dropped below the scores for animals pretreated with citrate buffer but remained significantly higher than the scores for transplanted animals that were not injected with 6-OHDA. Both histological and behavioral measures indicate GDNF partially protects integrated transplants against neurotoxic insult.
journal_name
Brain Resjournal_title
Brain researchauthors
Yurek DM,Fletcher-Turner Adoi
10.1016/s0006-8993(99)01921-6subject
Has Abstractpub_date
1999-10-16 00:00:00pages
21-7issue
1eissn
0006-8993issn
1872-6240pii
S0006-8993(99)01921-6journal_volume
845pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The main purpose of the present study was to determine whether specific lymphokines may be neurotrophic, by testing their effects on the survival of hippocampal neuronal cultures. Previous studies have shown that a variety of interleukins may be neurotrophic or neurotoxic, depending upon the culture conditions, as wel...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90400-h
更新日期：1993-01-08 00:00:00
abstract：:We studied the effects of nitric oxide (NO) and the NO-releasing agents sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP) and isosorbide dinitrate (ISDN) on N-methyl-D-aspartate (NMDA)-induced increases in intracellular Ca2+ ([Ca2+]i), whole-cell patch-clamp currents and on glutamate-stimulated [3H]di...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91690-g
更新日期：1992-10-02 00:00:00
abstract：:Zinc and other cations were iontophoretically applied to neurones in the frontoparietal cortex of urethane-anaesthetised rats and their effect on the activity of these cells was examined. Slow and often prolonged increases in firing rate occurred in 45.2% of neurones after zinc application and 72.7% after barium. Calc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90097-0
更新日期：1984-10-08 00:00:00
abstract：:In anaesthetized, spontaneously breathing cats and dogs, extracellular recordings were made of the spontaneous activities of 28 single neurones with cardiac rhythm. It was confirmed by histological examination that the neurones were situated in the mediodorsal portion of the nucleus of the solitary tract. Different di...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90049-x
更新日期：1977-09-09 00:00:00
abstract：:The visual system in the pigeon is composed of the tectofugal, thalamofugal and accessory optic pathways. Though their anatomy and physiology have been extensively studied, the functional interactions between these pathways are largely unknown. The present study shows by using multiple electrophysiological techniques ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.11.063
更新日期：2006-01-19 00:00:00
abstract：:The compound m-chlorophenylpiperazine (m-CPP) is used clinically to manipulate serotonergic function, though its precise mechanisms of actions are not well understood. m-CPP alters synaptic transmission and neuronal function in vertebrates by non-selective agonistic actions on 5-HT(1) and 5-HT(2) receptors. In this st...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02271-6
更新日期：2003-04-18 00:00:00
abstract：:Acute forebrain ischemia reduced protein kinase C (PKC) activity in the adult rat cortex, striatum and hippocampus by 60-70% after 20 min ischemia episodes, followed by 48 h of recirculation. Ischemia of 1 min, followed by recirculation, produced a less pronounced but significant decrease in PKC activity. The ischemia...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91094-h
更新日期：1991-02-08 00:00:00
abstract：:Auditory working memory (WM) is the cognitive faculty that allows us to actively hold and manipulate sounds in mind over short periods of time. We develop here a particular perspective on WM for non-verbal, auditory objects as well as for time based on the consideration of possible parallels to visual WM. In vision, t...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2016.01.044
更新日期：2016-06-01 00:00:00
abstract：:While moderate calorie restriction (CR) in the absence of malnutrition has been consistently shown to have a systemic, beneficial effect against aging in several animals models, its effect on the brain microstructure in a non-human primate model remains to be studied using post-mortem histopathologic techniques. In th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.02.046
更新日期：2013-05-01 00:00:00
abstract：:We tested the hypothesis that a single systemic injection of 380 mg/kg of the muscarinic agonist pilocarpine would produce more diffuse and severe seizure-induced brain damage than a single injection of lithium (3 mEq/kg) followed 4 h later by < 1/10 the dosage of pilocarpine. The hypothesis was not supported; the pat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91126-6
更新日期：1994-06-20 00:00:00
abstract：:This review summarizes the anatomical and functional organization of the frontal cortex of the rat in comparison to primates. Lesions of the primary motor or of the prefrontal cortex of both primates and rodents produce a consistent constellation of symptoms that are strikingly similar across species as diverse as rat...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/0165-0173(84)90018-3
更新日期：1984-11-01 00:00:00
abstract：:The results showed that the development of seizures (pentylenetetrazole, PTZ-induced kindling; PTZ was administered to rats at a subconvulsive dose of 35mg/kg three times a week, i.p.) was accompanied by a progressive recruitment of limbic structures and a characteristic pattern of changes in the brain tissue concentr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.04.045
更新日期：2010-06-25 00:00:00
abstract：:SC1 is an extracellular matrix glycoprotein that is related to the multifunctional protein SPARC. These matricellular members play regulatory roles in modulating cellular interactions. SC1 expression is enriched in the central nervous system during embryonic and postnatal development as well as in the adult brain. In ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02270-9
更新日期：2002-03-29 00:00:00
abstract：:The hypoxia tracer fluorine-18 fluoromisonidazole ([18F]FMISO) and its tritiated counterpart ([(3)H]FMISO) have been used as markers of potentially salvageable brain (ischemic penumbra) after stroke. In experimental models, the dynamics and half-life of [3H]FMISO allow concurrent histology after 24 h. Our aim was to f...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.06.102
更新日期：2009-09-08 00:00:00
abstract：:The postnatal development of acetylcholinesterase (AChE, EC 3.1.1.7) and NADH-diaphorase was examined in the caudate-putamen nucleus and substantia nigra of rats ranging from 3 to 90 days in age. From 3 to 15 days post partum islands of AChE and NADH-diaphorase activity were observed in the caudate-putamen nucleus. In...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)91022-2
更新日期：1976-04-23 00:00:00
abstract：:Morphine is one of the most potent analgesics used in medicine and it's long-term use is associated with the risk of the state of dependence. The cessation of chronic morphine administration leads to withdrawal signs which are associated with neurotransmitter dysregulations within mesolimbic system. Adenosine 5'-triph...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2019.05.025
更新日期：2019-09-15 00:00:00
abstract：:Converging evidence suggests that compromised motor abilities in hemiparetic cerebral palsy are not solely due to impairments in motor execution, but are also related to deficits in action planning. The present study had two aims. First, we compared grip planning in a sequential task between participants with left-sid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.12.074
更新日期：2009-03-19 00:00:00
abstract：:We immunocytochemically examined the effect of chronic salt loading on the content of calretinin, a calcium-binding protein, in both the supraoptic nucleus and the magnocellular parts of the hypothalamic paraventricular nucleus. In control rats that were given water for drinking, the supraoptic nucleus contained a clu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00003-9
更新日期：1995-03-06 00:00:00
abstract：:The maturation and growth of fetal serotonergic raphe neurons have been studied immunohistochemically and biochemically between 1 week and 5 months after grafting to the hippocampal formation in 5,7-dihydroxytryptamine-pretreated adult rats. The average number of surviving neurons in each group was 1800, which is equi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90490-8
更新日期：1988-08-16 00:00:00
abstract：:In order to investigate whether a fibrin-fibronectin-containing matrix of a peripheral regeneration chamber could promote the growth of central nervous system neurons, hippocampal and septal slices were co-cultured in the presence of this acellular substrate. In introducing the peripheral matrix into a 2-mm-long tube ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90506-q
更新日期：1991-02-01 00:00:00
abstract：:Dendrites were transected 100 microns from nerve cell somata. The probability of cell survival increased as a function of the extent of proximal segment retraction. The retraction effect is probably the result of increased resistance to calcium and sodium currents at the lesion. It is speculated that limitation of ner...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90526-9
更新日期：1987-11-10 00:00:00
abstract：:For the evaluation of glutamatergic and GABAergic transmission during seizures, rat hippocampal CA1 and CA3 areas were separately assessed by brain microdialysis, and extracelluar glutamate and GABA were measured through the course of the seizures after a systemic administration of kainic acid (KA). The generalized co...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00507-1
更新日期：1998-07-27 00:00:00
abstract：:The present study aimed to determine the kinetics of L-3, 4-dihydroxyphenylalanine (L-DOPA) uptake in an immortalised cell line of rat capillary cerebral endothelial cells (clones RBE 4 and RBE 4B), to define the type of inhibition produced by L-5-hydroxytryptophan (L-5-HTP), 2-aminobicyclo(2,2,1)-heptane-2-carboxylic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01387-6
更新日期：1999-05-22 00:00:00
abstract：:We investigated the effect of beta-estradiol (E2) on synaptogenesis in the hippocampus using organotypic hippocampal slice cultures and subregional hippocampal neuron cultures. E2 increased the expression of PSD95, a postsynaptic marker, specifically in stratum lucidum of Cornu Ammonis 3 (CA3SL) in cultured hippocampa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.02.093
更新日期：2007-05-30 00:00:00
abstract：:After a moderate global cerebral ischemia, two hypothetical populations of pyramidal neurons are present among the hippocampal CA1 pyramidal neurons: one that will die and another one that will survive. Prior analysis of dissected hippocampal CA1 regions has shown a reduction of the GluR1-3 mRNA following ischemia. In...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)01985-0
更新日期：2001-03-09 00:00:00
abstract：:The same cells in both the intact retina and retinal cultures of the rabbit retina take up exogenous serotonin or 5,7-dihydroxytryptamine. Both substances can be localised by immunohistochemistry using a monoclonal antibody directed against serotonin. The 5,7-dihydroxytryptamine can also be revealed directly in both f...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90125-3
更新日期：1986-09-10 00:00:00
abstract：:Substance P(SP), the heptapeptide SP and the stable analogue (p-Glu5-MePhe8-MeGly9) SP (DiMe-C7) induce a Ca2+-dependent release of Met5-enkephalin (MET) from slices of periaqueductal gray matter (PAG) and striatum of rats. The MET release from striatal slices is greater than that from PAG slices because of the higher...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90169-5
更新日期：1983-11-21 00:00:00
abstract：:Purkinje cell degeneration (pcd) mutant mice lose virtually all Purkinje cells. It was found that the pcd mutants did not show within-sessions habituation in terms of hole-poking responses. This type of perseverative responding has been found in rats with limbic system damage and implies a role for the cerebellum in b...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91354-1
更新日期：1989-02-06 00:00:00
abstract：:Epileptic discharge patterns in an acute experimental model of epilepsy were analyzed. Epileptic foci were induced by epicortical application of penicillin on the rat motor cortex in vivo. Patterns with regular 1/s discharges, patterns with irregular discharges of about 0.5/s as well as compound patterns comprising di...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90221-8
更新日期：1993-07-09 00:00:00
abstract：:The putative involvement of the dopaminergic innervation of the medial part of the prefrontal cortex (PFC) in short-term memory functioning was investigated by evaluating the effects of local infusions of dopaminergic drugs into the ventral part of the medial PFC of rats in an operant delayed-matching-to-position (DMT...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00025-l
更新日期：1995-03-20 00:00:00
