Abstract：
:Morphine is one of the most potent analgesics used in medicine and it's long-term use is associated with the risk of the state of dependence. The cessation of chronic morphine administration leads to withdrawal signs which are associated with neurotransmitter dysregulations within mesolimbic system. Adenosine 5'-triphosphate (ATP) and purinergic system play an important role in the activity of central nervous system (CNS). Purinergic receptors are widely distributed in neurons and glial cells throughout the CNS taking part in integration of functional activity between neurons, glial and vascular cells. In the present study the mRNA and protein expression of purinergic P2X4 and P2X7 receptors in selected mesolimbic structures (striatum, hippocampus and prefrontal cortex) during morphine withdrawal in rats was investigated by RT-PCR and Western Blot analysis. Two experimental models of morphine withdrawal were studied: single and repeated morphine withdrawal. We demonstrated that expression of P2X4 and P2X7 receptors was altered during morphine withdrawal period in rats. These alterations were varied in particular mesolimbic areas depending on the scheme of morphine administration. Our results extend the current knowledge on morphine withdrawal and for the first time high-light interactions between purinergic system and morphine withdrawal. It seems, the purinergic system may be a new, valuable tool in searching for a new strategy of management of opioid dependence.
journal_name
Brain Resjournal_title
Brain researchauthors
Metryka E,Gutowska I,Kupnicka P,Tarnowski M,Tkacz M,Listos J,Talarek S,Barczak K,Chlubek D,Baranowska-Bosiacka Idoi
10.1016/j.brainres.2019.05.025subject
Has Abstractpub_date
2019-09-15 00:00:00pages
49-56eissn
0006-8993issn
1872-6240pii
S0006-8993(19)30280-Xjournal_volume
1719pub_type
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