Abstract：
:Apoptotic cell death, in conjunction with mitosis, plays an important role in maintaining tissue homeostasis. Many cancer treatment modalities have been shown to induce apoptosis in sensitive cells. The purpose of this study was to determine the amount of apoptosis in-situ, the expression of apoptosis related genes p53 and bcl-2 and the proliferative marker nucleolar organiser region (AgNOR), in squamous cell carcinoma (SCC) of the larynx and correlate the results to clinical outcome. Paraffin-embedded samples of 66 patients with laryngeal SCC (34 glottic, 24 supraglottic and 8 transglottic) treated at Turku University Hospital were re-examined and divided into three histological grades of differentiation, four grades of keratinisation, and four grades of p53 and bcl-2 immunostaining. The apoptosis in-situ was assessed by TUNEL and was analysed as the number of apoptosis per volume corrected high power fields. The percentages of cells containing tumour nuclei with one to more than four AgNORs were counted. The patient median age was 65, the disease-free 5-year survival was 53% and the overall survival rate was 64%. In univariate analysis, nodal status, tumour size and general condition were associated with disease-free and overall survival. In a subgroup of patients with T1-2N0 glottic cancer receiving definitive irradiation therapy (n = 25), small tumour size and good histological differentiation were associated with good disease-free and overall survival. Apoptosis associated and proliferative markers did not seem to have more value to prediction of clinical outcome than the common clinical parameters.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Pulkkinen JO,Klemi P,Martikainen P,Grénman Rsubject
Has Abstractpub_date
1999-01-01 00:00:00pages
703-7issue
1Beissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract：BACKGROUND:In our previous study, it was suggested that nitrotyrosine, a product of nitrogen species, found in esophageal squamous cell carcinoma (ESCC), may contribute to the progression of esophageal cancer. MATERIALS AND METHODS:To clarify whether nitrotyrosine expression is associated with apoptosis and/or angioge...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The in vitro chemosensitivity of MAC 15A ascites cells to CNC-alanylalanine and CNC-glycinemethylamide was assessed using a clonogenic assay system. In vitro stability studies and in vivo pharmacokinetics were performed using a reversed-phase HPLC technique. Initial concentrations of CNC-alanylalanine and CNC-glycinem...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Macrophages are thought to be the cells that initially respond to environmental information and transmit this information to other immune cells. We hypothesize that there is a "network system" consisting of various tissue macrophages; the macrophages respond to stimulation and transmit secondary information to neighbo...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2006-11-01 00:00:00
abstract：BACKGROUND:The purpose of the present study was to correlate the nuclear expression of p53 and p21 with response to paclitaxel and carboplatin, progression-free survival (PFS) as well as overall survival (OS), in patients with urothelial metastatic transitional-cell carcinoma (TCC). PATIENTS AND METHODS:Histological s...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Extracts from Viscum album L. (VAL), which are one of the most widely used alternative cancer therapies in Europe, have been recognised to induce apoptosis. A clinically relevant direct anti-tumour effect might be induced only by their intratumoural or intrapleural injection, and thus effectiveness is highly...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:One of the challenges to improving access to care is identifying disparities in health care. To determine the influence of insurance status on outcome for patients with non-small cell lung cancer (NSCLC), we analyzed data from the National Cancer Data Base (NCDB) from 1998-2011. MATERIALS AND METHODS:Data f...
journal_title：Anticancer research
pub_type： 历史文章,杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma. MATERIALS AND METHODS:We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and im...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11226
更新日期：2016-12-01 00:00:00
abstract：:The immunomodulatory and antimetastatic/antitumor activity of thymic humoral factor-gamma 2 (THF-gamma 2) was evaluated in BALB/c-mice. Daily subcutaneous applications (7 consecutive days, 20, 200 ng of THF-gamma 2 per injection/mouse) upregulated counts of thymocytes and peripheral blood cells in tumor bearing mice. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Cyclin D1 is a key protein involved in cell cycle regulation. A common A870G single nucleotide polymorphism in exon 4 of the cyclin D1 gene (CCND1) has an effect on the transcription of two different cyclin D1 mRNAs. Association of the genetic polymorphism of A870G of CCND1 with several cancer risks has been...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:The cytosol hormone receptors were extracted from rabbit uteri and human tumors with tris buffer systems for three consecutive homogenization and centrifugation processes to obtain three cytosols C-1, C-2, and C-3. The assay results evaluated revealed that minimal to tremendous amounts of receptor activities were pres...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:The aggressive nature of gliomas is closely related to their capacity to evade the anti-tumoral immune response. The mechanisms implicated in this phenomenon are only partially understood. A subset of T cells, termed CD4+ CD25+ regulatory T cells (Treg), have been shown to inhibit the actions of effector lymphocytes. ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2008-03-01 00:00:00
abstract：:Receptors for peanut agglutinin (PNA) were isolated from Lewis lung carcinoma cells (3LL) by detergent solubilization and affinity chromatography on PNA-agarose. The isolated receptors showed heterogeneous yet distinct molecular species when they were analyzed by SDS gel electrophoresis. In vivo inoculation of the iso...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:We herein report a case of locally advanced human epidermal growth factor receptor 2 (HER2)-positive breast cancer that achieved a pathological complete response (pCR) with pertuzumab, trastuzumab and docetaxel therapy. A 70-year-old female presented with an elastic hard mass, 5.0 cm in diameter with broad redness and...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11530
更新日期：2017-04-01 00:00:00
abstract：:In the clinical setting, administration of high daily or bolus doses of vitamin D is often solely based on 25-hydroxyvitamin D [25(OH)D] testing. This review summarizes the evidence of the effect of vitamin D on cardiovascular disease (CVD). Meta-analyses of randomized controlled trials (RCTs) have demonstrated that C...
journal_title：Anticancer research
pub_type： 杂志文章,meta分析,评审
doi：10.21873/anticanres.13643
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND:Drug resistance to 5-fluorouracil (5-FU) is a major obstacle in colonic cancer treatment. Activation of nuclear factor-kappa B (NFκB), mitogen-activated protein kinase kinase kinase 8 (MAP3K8) and protein kinase B (AKT) is thought to protect cancer cells against therapy-induced cytotoxicity. MATERIALS AND M...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:Patients with an oral squamous cell carcinoma (OSCC) often develop multiple malignant lesions. This report examined whether individual tumours developed in a patient show the same genetic alteration, such as p53 mutations. This case study describes three SCCs and three leukoplakias which developed simultaneously in a ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：:Treatment of patients with glioblastoma improved dramatically when concomitant and adjuvant temozolomide was added to external radiation therapy. The addition of this new treatment schedule as well as the improvements in individually-tailored radiation treatment, has resulted in a larger proportion of patients being f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：BACKGROUND:Parathyroid hormone-related protein (PTHrP) is a key regulator of osteolytic metastasis of breast cancer (BC) cells, but its targets and mechanisms of action are not fully understood. This study investigated whether/how PTHrP (1-34) signaling regulates expression of vascular endothelial growth factor (VEGF) ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND:Glioblastoma multiforme is the most common and most aggressive type of primary brain tumor, accounting for 52% of all primary brain tumor cases and 20% of all intracranial tumors. Recently, evidence for a viral cause has been postulated, possibly SV40 or more likely cytomegalovirus (CMV). One report indicate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：UNLABELLED:Lesions containing somatostatin receptors (SSR) in rats and in man can be visualized in vivo using radiolabeled octreotide (OCT) analogs. SSR scintigraphy was initially performed with [123I-Tyr3]OCT and later with [111In-DTRA0]OCT. With the latter the residence time of radioactivity (111In) in SSR-positive t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The present study compared the effects of four lipophilic forms of selenium with regard to cancer chemopreventive activity, tissue selenium accumulation, and bioavailability for synthesis of a selenoprotein. These reagents included methylphenyl selenide, diphenyl selenide, triphenyl-selenonium chloride, and p-xylylbis...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND:Interleukin 32 (IL32) is an intracellular pluripotent cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells and seems to be involved in the pathogenesis of cancer and inflammatory diseases. Our purpose was to assess the role of protein expression and genetic polymorphisms o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12225
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND/AIM:Silica nanoparticles (nano-SiO2) are widely used in many industrial areas and there is much controversy surrounding cytotoxic effects of such nanoparticles. In order to determine the toxicity and possible molecular mechanisms involved, we conducted several tests with two breast cancer cell lines, MDA-MB-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12068
更新日期：2017-11-01 00:00:00
abstract：BACKGROUND:Endometrial carcinoma is a well known complex gynecological disorder. Our team suggests that this tumor is related to immortalization-up-regulated protein 1 and 2 (imup-1, imup-2), which are known to be involved in SV40-mediated immortalization. PATIENTS AND METHODS:RT-PCR and immunohistochemical staining w...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:Natural antibodies directed against CEA and a related antigen, NCA, have been demonstrated in all normal and pathological sera using an EIA, although they have never been detected by RIA. These antibodies are not anti-blood group antibodies, as their titer was decreased only slightly by absorption with blood group sub...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Grape seed extracts were more cytotoxic than grape peel extracts. Methanol and 70% methanol extracts of grape seed selectively killed two human oral tumor cell lines, more efficiently than human gingival fibroblasts. ESR spectroscopy revealed that these extracts produced radicals under alkaline conditions and enhanced...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：AIM:To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:MUC1 (DF3 antigen) is a member of a family of high molecular weight glycoproteins. Recent studies have demonstrated that MUC1 is expressed in tumors of various human organs and may function as an anti-adhesion molecule that inhibits cell-to-cell adhesion, inducing tumor metastasis. However, expression patterns of MUC1...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:Peripheral blood cells of cancer patients with advanced renal carcinoma and treated with interleukin-2 by intravenous application were studied by electron microscopy at different intervals from the beginning of rIL-2 administration. Morphofunctional modifications of lymphocytes, circulating hystiocytes containing phag...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Chloroform (CHCl3) is an established rodent carcinogen and a prevalent contaminant of chlorine-disinfected drinking water. Thus in the United States CHCl3, along with other trihalomethanes, is regulated not to exceed 100 ppb in potable water. Recently, several studies have shown that CHCl3 also has anti-cancer propert...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
