Abstract：
BACKGROUND:Extracts from Viscum album L. (VAL), which are one of the most widely used alternative cancer therapies in Europe, have been recognised to induce apoptosis. A clinically relevant direct anti-tumour effect might be induced only by their intratumoural or intrapleural injection, and thus effectiveness is highly dependent on the apoptosis-inducing potencies of the extracts used. MATERIALS AND METHODS:To characterise these properties, human lymphocytes were incubated for 72 hours with VAL extracts from different host trees and harvest times. Cell death-associated changes such as an apoptotic sub-G1 peak, binding of Annexin-V, generation of reactive oxygen intermediates (ROI), expression of mitochondrial Apo2.7 molecules, and uptake of the DNA-intercalating dye propidium iodide were measured by flow cytometry. RESULTS AND CONCLUSIONS:Biologically active VAL compounds, and thus cytotoxicity, are dependent on the manufacturing process, host tree, and time of harvest. Although the mistletoe lectin (ML) content of VAL extracts strongly correlated with their apoptosis-inducing properties, the presence of these proteins will not guarantee its biological activity, indicating the involvement of other components which may modulate ML cytotoxicity.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Büssing A,Schietzel Msubject
Has Abstractpub_date
1999-01-01 00:00:00pages
23-8issue
1Aeissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract：:The aim of our study was to establish a simple method for routine analysis of c-erbB-2 gene expression in tumour samples. We constructed a plasmid for the in vitro synthesis of competitor RNA to be used as an internal exogenous control during the RT-PCR detection of c-erbB-2 expression. The competitor RNA harbors a 19...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:Genetic polymorphisms of carcinogen-metabolizing enzyme genes have been associated with the risk of oral squamous cell carcinoma and oral leukoplakia. The overexpression of p53 protein is the most common genetic alteration in head and neck cancer. In the present study the combined or isolated presence of glu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:A 56-year-old Caucasian smoker male patient underwent right orchiectomy and spermatic cord resection for intrascrotal transilluminating mass. Histology findings suggested diagnosis of metastasis from Renal Cell Carcinoma (RCC). After positive CT-scan for primary neoplasm, total left nephrectomy was accomplished. Patie...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
abstract：AIM:Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI). MATERIALS AND METHODS:Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：AIM:Metabolic syndrome has been identified as a risk factor for colorectal cancer and adenoma. The aim of our study was to assess the risk of colorectal cancer and adenoma in an adult Italian population with metabolic syndrome. PATIENTS AND METHODS:Ninety patients with metabolic syndrome were prospectively compared ag...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：:In medullary carcinoma of the thyroid (MTC), multidrug resistance (MDR) remains the major obstacle to effective chemotherapy. In this work MDR was investigated in TT cells, a human MTC cell line. We studied the effect of an efficient MDR agent (SDZ PSC 833) on doxorubicin (DOX)-induced cytotoxicity in TT cells culture...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND:The chronomodulated infusion of 5-FU, FA and oxaliplatin allows a significant increase in dose intensity and antitumor efficacy in patients with metastatic colorectal cancer. Here we investigated if substitution of oxaliplatin with cisplatin produced a similar antitumor activity in previously untreated patie...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:The effects of two gallium (Ga) compounds, Ga chloride (GaCl3) and tris(8-quinolinolato)Ga (III) on the viability of A549 human malignant lung adenocarcinoma cells were investigated. The results demonstrated that both drugs reduced the viability of A549 cells but to different extents. The inhibitory effects of tris(8-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:This was a validation study of a regional register of oral cancer in Örebro, Sweden. The purpose was to assess the rate of errors in baseline, and treatment, and the completeness and accuracy of data on recurrences. MATERIALS AND METHODS:A total of 653 cases with squamous cell cancer in the oral cavity were...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13109
更新日期：2019-01-01 00:00:00
abstract：:Mammalian cells transfected with the S. cerevisiae APN1 protein acquire resistance to oxidizing agents, the damage of which is mainly repaired via DNA base excision repair (BER). We have recently hypothesized that this effect might be linked to the possible capacity of APN1 to accelerate mammalian BER by its 3' dieste...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND:Concurrent chemoradiotherapy (CCRT) is used to treat advanced head and neck cancer. The accuracy of evaluating lymph nodes metastases following CCRT is important for subsequent therapy. PATIENTS AND METHODS:Patients were divided into two groups, the complete response (CR) and the non-CR groups, as determine...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND/AIM:ANRIL is a long noncoding RNA located on INK4 locus, which encodes p15 and p16 that cause G1 phase arrest in the cell cycle. ANRIL positively regulates proliferation of several kinds of cancer cells such as lung and gastric cancers. This study, examined the effect of ANRIL in head and neck squamous cell ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13564
更新日期：2019-08-01 00:00:00
abstract：:In order to improve the treatment of advanced H&N cancer we must consider to adopt new strategies as: new/better cytostatic agents; new combinations of present cytostatic agents and; potentiation of radiotherapy and cytostatic agents by biochemical modulation, which we define as potentiation of therapy by non-chemothe...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1993-11-01 00:00:00
abstract：AIM:The aim of this study was to investigate the effects of medium-chain triglycerides (MCTs) on chemically-induced hepatic carcinogenesis (HCC) in mice. MATERIALS AND METHODS:In a first set of experiments, mice were treated with diethylnitrosoamine intraperitoneally at two weeks of age. They were fed chow containing ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13880
更新日期：2019-12-01 00:00:00
abstract：:The requirements on sunscreens have essentially changed, since some years ago it was demonstrated that approximately 50% of free radicals, that are formed in the skin by solar radiation, originate from the visible and infrared regions of the solar spectrum. In addition, a critical radical concentration threshold could...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:There is little effective therapy for patients with hormone-refractory breast cancer. Hormone resistance is frequently due to the transcriptional inactivation of the estrogen receptor (ER) gene. We determined the effect of DHAC, a cytosine DNA methyltransferase (CMT) inhibitor, on the estrogen sensitivity in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:C-reactive protein (CRP) is the prototypical biomarker of inflammation. Genetic variations within the CRP gene have been shown to be associated with alterations of CRP expression and prognosis in cancer patients. The purpose of this study was to evaluate the association between four polymorphisms of the CRP ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:The prognostic significance of angiogenesis, proliferation and apoptosis after interferon (IFN) was investigated in 96 patients with renal cell carcinoma (RCC). MATERIALS AND METHODS:Microvessel density in the tumor(MVD), proliferative index(PI) and apoptotic index(AI) were quantified using immunohistochemi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND/AIM:The purpose of this study was to investigate the clinical, pathological, and prognostic differences between adenocarcinoma (ADC) and mucinous adenocarcinoma (MUC) in colorectal cancer (CRC). PATIENTS AND METHODS:This was a retrospective study of a Japanese high-volume cancer Center over a 10-year period...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14115
更新日期：2020-03-01 00:00:00
abstract：:Human epidemiological studies suggest an association between N-acetyltransferase (NAT) activity and the incidence of bladder and colorectal cancers. In this study, paclitaxel was selected to examine the inhibition of arylamine NAT activity, gene expression and 2-aminofluorene-DNA adduct formation in a human osteogenic...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND/AIM:We evaluated the usefulness of reverse transcription-polymerase chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) translocations using cytology samples from lung cancer patients. MATERIALS AND METHODS:We analyzed ALK translocations by RT-PCR in cytology samples from lung cancer pati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11696
更新日期：2017-06-01 00:00:00
abstract：AIM:The expression level of DNA repair-related genes and their association with breast cancer status among participants of the New York site of the Breast Cancer Family Registry was investigated. MATERIALS AND METHODS:RNA from mononuclear cells in 194 sister sets (n=475 women) were assayed for ATM, BRCA1, MSH2, MUTYH ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND:Cyclooxygenase (COX) is a key enzyme in the conversion of arachidonic acid to prostaglandins and other eicosanoids. A recent report has indicated a selective COX-2 inhibitor resulted in increased apoptosis and down-regulated bcl-2 expression in the androgen-sensitive human prostate cancer cell. We investigat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：BACKGROUND:Women with recurrent ovarian cancer have a poor prognosis and short survival. However, some women are long-term survivors and it is unclear whether they share specific common characteristics. CASE REPORT:We present the case of a 63-year-old woman with histologically-proven recurrent ovarian cancer and a sur...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11110
更新日期：2016-10-01 00:00:00
abstract：:Cathepsin B (CB) is involved in degradation of extracellular matrix proteins during tumor progression in human solid organ tumors (such as colorectal, bladder, and breast cancers), including human prostate cancer. Its activities are regulated by endogenous inhibitors (such as stefins or cystatins). Increased expressio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：AIM:To evaluate the proliferation activity in gliomas using 18F-fluorothymidine (18F-FLT)-positron emission tomography/computed tomography (PET/CT). PATIENTS AND METHODS:Samples of 26 tumors were analyzed (mean age=51.6; range=26-72 years; 16 males, 10 females). All examinations were performed using a PET/CT scanner e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：:25 consecutive leukemia patients (21 AML, 4 ALL) with either primary resistance (n = 22) or resistant relapse (n = 3) of all FAB-subtypes were treated with 1 or 2 cycles of ID-ara C (1 g/m2 i.v. q 12h days 1-6) and AMSA (120 mg/m2 i.v. days 5-7). Patients reaching CR received 1 cycle of intensive consolidation using a...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Metastatic pancreatic malignant melanoma is considered to be a highly aggressive neoplasm, and only few metastasectomies for lesions originating from the skin or the ocular region have been reported. We report a case of resection of pancreatic metastasis of malignant melanoma originating from the nasal cavity. An isol...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2013-02-01 00:00:00
abstract：:Initial events in the metastatic spread of tumours involve loss of cell-cell adhesion within the primary tumour mass. The integrity and morphology of epithelial tumour cell colonies is maintained primarily by cell-cell adhesions mediated by E-cadherin and its associated intracellular catenin molecules. Hepatocyte grow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:We have recently demonstrated that Thymax, a gross thymic extract, induces an apoptotic effect against human breast cancer cells. In this study, the ability of Thymax to activate human dendritic cells (DCs) and the DC-directed T-cell response was examined in an in vitro culture model of peripheral blood mono...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
