Abstract:
BACKGROUND:Drug resistance to 5-fluorouracil (5-FU) is a major obstacle in colonic cancer treatment. Activation of nuclear factor-kappa B (NFκB), mitogen-activated protein kinase kinase kinase 8 (MAP3K8) and protein kinase B (AKT) is thought to protect cancer cells against therapy-induced cytotoxicity. MATERIALS AND METHODS:Using cytotoxicity assays and immunoblotting, the impact of inhibitory strategies addressing NFκB, AKT and MAP3K8 in chemoresistance was evaluated in a colonic cancer model in vitro. This model consisted of the cell lines SW480 and SW620, and three subclones with increasing degrees of chemoresistance in order to mimic the development of secondary resistance. RESULTS:NFκB protein p65 was selectively activated in all resistant cell lines. Consequently, several inhibitors of NFκB, MAP3K8 and AKT effectively circumvented this chemoresistance. As a cellular reaction, NFκB inhibition may trigger a feedback loop resulting in activation of extracellular signal-regulated kinase. The results suggest that chemoresistance to 5-FU in this colonic carcinoma model (cell lines SW480 and SW620) is strongly dependent on NFκB activation. The efficacy of MAP3K8 inhibition in our model potentially uncovers a new mechanism to circumvent 5-FU resistance.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Körber MI,Staribacher A,Ratzenböck I,Steger G,Mader RMsubject
Has Abstractpub_date
2016-04-01 00:00:00pages
1631-9issue
4eissn
0250-7005issn
1791-7530pii
36/4/1631journal_volume
36pub_type
杂志文章abstract::Urachal cancer is a rare malignancy and the standard treatment is surgical resection. The prognosis of recurrent and metastatic urachal cancer is extremely poor because there is no established chemotherapy regimen. Here, the response of one patient with recurrent urachal cancer to combination chemotherapy of gemcitabi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::We have previously reported that the presence of an extracapsular invasion (ECI) at sentinel lymph nodes (SLNs) is a strong predictor of non-SLN metastasis in breast cancer. We hypothesized that(18)F-fluorodeoxyglucose (FDG) uptake by metastatic SLNs reflects invasive disease, or ECI. In this study, we evaluated the a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract::We examined 11 patients (9 male and 2 female, age 41 +/- 13) presenting astrocytomas of different malignancy grade (WHO) with single voxel (PRESS) and double voxel (STABLE) spectroscopy techniques. The content of choline in the tumors was determined as a function of their grade of malignity and a significant correlati...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::The proteasome inhibitor, bortezomib, is known to be effective in the therapy of various neoplasms. The objective of this study was the analysis of the ex vivo activity of bortezomib in paediatric acute lymphoblastic leukaemia (ALL), in comparison to paediatric acute myeloid leukaemia (AML). A total of 159 patients en...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract::Two monoclonal antibodies, KM227 (IgM) and KM432 (IgG1), were established by immunizing mice with the crude membrane fraction of lung squamous cell carcinoma and the high-molecular-weight antigen in pleural perfusion from patients with lung adenocarcinoma, respectively. They were found to recognize the carbohydrate ep...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:The hedgehog signaling pathway (HH) is involved in tumorigenesis in a variety of human malignancies. In head and neck squamous cell carcinomas (HNSCC), Hh overexpression was associated with poor prognosis. Therefore, we analyzed the effect of Hh signaling blockade with cyclopamine on colony formation of cell...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
abstract::Human urothelial cell lines of non-malignant and malignant origin were classified into three grades of transformation, i.e. TGr I, TGr II, and TGr III, which differ from primary and early cultures of normal bladder mucosa (TGr 0) by growth in the absence of fibroblasts, prolonged survival, increasing chromosomal abnor...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Chronic lymphocytic leukaemia remains the most common adult leukaemia in Western countries. In this review we tried to give an overview of the important prognostic markers and the developments in this field. We also reflected about the accumulative character of the disease and the influence of the micro-environment on...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:The aim of this work was to study the constituents and cytotoxicity of the essential oils from the fruits of Angelica archangelica growing in Iceland. MATERIALS AND METHODS:Three samples of essential oils were prepared by steam distillation. Their composition was established with GC/MS. The effects of the o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND:Mifepristone has been demonstrated to improve longevity and quality of life in mice with spontaneous murine cancer without progesterone receptors and in human colon cancer. The present study evaluated the palliative effect of mifepristone in a variety of different types of human cancer. PATIENTS AND METHODS...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::The mouse/human chimeric antibody Ch F11-39, recently generated by ourselves, shows the same high specificity and affinity for carcinoembryonic antigen (CEA) as those of its parental mouse monoclonal antibody. Ch F11-39 is capable of mediating antibody-dependent cell-mediated cytotoxicity (ADCC) with human peripheral ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Since the anti-HER2 monoclonal antibody trastuzumab made its triumphant advance into breast cancer therapy, new selective agents, including pan-HER inhibitors are entering clinical practice. MATERIALS AND METHODS:This study investigates the expression of the four HER-family members, HER1-4, in 48 primary br...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND/AIM:N6-Methyladenosine (m6A), the most abundant internal modification of RNA, plays a critical role in cancer development. However, the clinical implications of m6A in hepatocellular carcinoma (HCC) remain unclear. MATERIALS AND METHODS:We analyzed 177 HCC and paired noncancerous liver tissues from patients...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14690
更新日期:2020-12-01 00:00:00
abstract::A consecutive series of 128 breast cancer patients detected by breast screening were enrolled in this study to analyze their age-dependent characteristics. They were classified into three groups; 40 years or less (young): 26 patients, 41-55 years (middle-aged): 67 patients and 56 years or over (old): 35 patients. The ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:Reduced serum-zinc concentrations are well known as typical laboratory characteristics in advanced head and neck cancer. Our aim was to follow the development of this phenomenon during the disease. PATIENTS AND METHODS:A total of 21 patients were included in this pilot-study (1 female, 20 male). The median ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:PS2-expression is controlled by estrogens and is a prognostic factor for long-term and post-relapse survival. Breast carcinomas associated with a BRCA1 mutation (BRCA1-BC'S) exhibit many specific features, particularly for ER expression. Our purpose was to determine if pS2-expression was different in BRCA1-B...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::Ten cell sublines the MDA-MB 231 human breast cell line were analyzed for their phenotypic diversity: morphology, karyotype, growth rate, clonogenicity in semisolid medium, tumorigenicity in nude mice, number and affinity of nuclear receptors for oestradiol, progesterone and glucocorticoids. Karyotypic analysis showed...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Between 1975 and 1982, 202 previously untreated patients with multiple myeloma (MM) and clinical evidence of disease progression received intensive chemotherapy every 3-5 weeks to induce remission. Treatment regimen included intermittent courses of a combination of cycle non-specific drugs (melphalan, cyclophosphamide...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Thymidylate synthase (TS) is a target enzyme of 5-fluorouracil (5-FU). TS has a polymorphic repeated sequence in the 5'-UTR and the polymorphism is associated with TS protein expression. TS polymorphism has been reported to link with the efficacy of 5-FU-based chemotherapy in colorectal cancer. In this study, we exami...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND/AIM:Breast cancer treatment represents a substantial amount of health-care costs and has a negative impact on womens' psychological health. Day-Surgery managment (DS) is a favorable alternative to a classic inpatient setting. In our prospective study we evaluated DS-treatment feasibility in terms of patient ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13451
更新日期:2019-06-01 00:00:00
abstract::The effect of alpha-Interferon (alpha-IFN), gamma-interferon (gamma-IFN), tumor necrosis factor alpha (TNF-alpha) and interleukin-2 (Il-2) on growth of two renal cancer cell lines (DNT-11, HTB-44) was studied. Cell proliferation was measured by a tetrazolium based microculture assay (MTT) after incubation of tumor cel...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Autophagy is an evolutionarily-conserved catabolic process which furthers cell survival, especially in times of nutritional stress. Whilst being by itself a pro-survival mechanism, it has many areas of overlap with apoptosis. Autophagic cell death is recognised as a subset of programmed cell death, with features more ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract::The response of the blood-forming and supportive stromal compartments of the marrow to a localized, fractionated course of radiotherapy [FxRT; 2.0 Gy (q24 h x 5)-->74 Gy] was examined in the C57Bl/6 mouse using the hematopoietic progenitor and the cobblestone-forming cell (CAFC) assays as end-points for the blood-form...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Late developing breast cancer metastases are common and lethal despite treatment with adjuvant chemotherapy at the time of primary tumor excision. Stimulation of an antitumor immune response is an alternative strategy for preventing this devastating development. MATERIALS AND METHODS:A mouse model of the hu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13029
更新日期:2018-12-01 00:00:00
abstract::Angiogenesis is critical for tumor growth and inflammation. Shiunko is a Chinese herbal ointment used for the treatment of burns in Japan. Its main ingredient is the root of Lithospermum erythrorhizon, which had been used for treating tumors and inflammation in China since the 5th century. We report here that shikonin...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::cis-Diamminedichloroplatinum(II) (CDDP) is the most active anticancer agent. It has been reported that intracellular accumulation of CDDP is an important step as a determinant for resistance to CDDP, which may be modulated by Na+, K(+)-ATPase activity. In this study, the significance of membrane Na+, K(+)-ATPase activ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:Endometrial carcinoma is a well known complex gynecological disorder. Our team suggests that this tumor is related to immortalization-up-regulated protein 1 and 2 (imup-1, imup-2), which are known to be involved in SV40-mediated immortalization. PATIENTS AND METHODS:RT-PCR and immunohistochemical staining w...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:Radiotherapy (RT) with or without chemotherapy (CT) plays an important role as exclusive treatment in patients with head and neck squamous cell cancer (HNSCC). Unfortunately, in some cases, benefit for patients is not recorded and only treatment-related complications are registered. MATERIALS AND METHODS:Da...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::The recombinant plasmid pBHIV-1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the reported chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants were obtained which were resistant to genetecin and expressed ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::For the selection of patients responding well to lentinan, we performed two immunological analysis of patients. Flow cytometric analysis was obtained from peripheral blood lymphocytes; the patients who had greater than 2.5 ratio of [CD11(-) CD8(+)/CD11(+) CD8(+) after treatment]/[CD11(-) CD8(+)/CD11(+) CD8(+) before t...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-07-01 00:00:00