Abstract：
BACKGROUND:Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma. MATERIALS AND METHODS:We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and immortalized keratinocyte (HaCaT) cells exposed to various isothiocyanates, under different experimental conditions. RESULTS:An experimental in vitro model utilizing low isothiocyanate concentrations (0.1-5 μM for 48 h with all treatments being refreshed after 24h) was shown to be (i) most efficient in exerting an anti-cancer effect when compared to higher concentrations (5-100 μM for 24 or 48 h added as a single bolus) and (ii) specific to A375 cells while A431 and HaCaT cells remained unaffected. Such effect involved the activation of several caspases including (iii) initiator caspases 8, 9, 4 (indicating the involvement of intrinsic, extrinsic and endoplasmic reticulum-based pathways) and (iv) effector caspases 3, 7 and 6. CONCLUSION:Utilization of low isothiocyanate concentrations (under the conditions described herein) exerts an anti-cancer effect specific to human malignant melanoma cells thus providing a therapeutic basis for their utilization in management of the disease.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Mantso T,Sfakianos AP,Atkinson A,Anestopoulos I,Mitsiogianni M,Botaitis S,Perente S,Simopoulos C,Vasileiadis S,Franco R,Pappa A,Panayiotidis MIdoi
10.21873/anticanres.11226subject
Has Abstractpub_date
2016-12-01 00:00:00pages
6303-6309issue
12eissn
0250-7005issn
1791-7530pii
36/12/6303journal_volume
36pub_type
杂志文章abstract：:Combined treatment of interleukin-2 (IL-2), 5-fluorouracil (5-FU) and mitomycin C (MMC) is reported to be effective against hepatic metastasis of colon carcinoma. To clarify this modality, we investigated lymphokine activated killer (LAK) activity of human peripheral lymphocytes on colon cancer cell lines by 51Cr rele...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Pure or metaplastic squamous cell carcinoma (SCC) of the breast is a rare entity with an unclear pathogeny and aggressive clinical behaviour. An attempt was made to characterize its differential immunohistochemical and biological profile. PATIENTS AND METHODS:Twenty-seven cases of SCC (pure or not) of the b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The association of anaemia and other predictors of fatigue was studied in cancer patients on palliative treatment. A cohort of 128 consecutive patients (61 men and 67 women, mean age 63.6 years; range 36-85) was interviewed using the Edmonton Symptom Assessment System (ESAS) questionnaire, with 11 items describing can...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：AIM:The American thyroid association (ATA) recommends that additional imaging procedures supplement cervical ultrasonography (US) in any patient with a basal calcitonin value above 150 pg/ml in the follow-up of medullary thyroid carcinoma (MTC). The aim of the present study was to reaffirm or challenge this cut-off for...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:Bone defects due to malignant tumor often lead to repeated surgery or amputation. Thus, a major objective in orthopedic surgery is the extracorporeal devitalization of tumor-bearing bone segments following reimplantation. Extracorporeal irradiation or autoclaving are possible methods even though they may cau...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2013-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1988-09-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2009-06-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
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doi：
更新日期：1994-11-01 00:00:00
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pub_type： 临床试验,杂志文章
doi：
更新日期：2007-07-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2009-08-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
