Abstract：
BACKGROUND:Parathyroid hormone-related protein (PTHrP) is a key regulator of osteolytic metastasis of breast cancer (BC) cells, but its targets and mechanisms of action are not fully understood. This study investigated whether/how PTHrP (1-34) signaling regulates expression of vascular endothelial growth factor (VEGF) produced by BC cells. MATERIALS AND METHODS:A mouse model of bone metastasis was prepared by inoculating mice with tumour cell suspensions of the human BC cell line MDA-MB-231 via the left cardiac ventricle. VEGF expression was examined by Western blot and real-time RT-PCR analysis, as well as by confocal microscopy in the bone microenvironment. RESULTS:PTHrP was expressed in cancer cells producing PTH/PTHrP receptor and VEGF that had invaded the bone marrow, and PTHrP was up-regulated VEGF in MDA-MB-231 in vitro. The culture medium conditioned by PTHrP-treated MDA-MB-231 cells stimulated angiogenesis and osteoclastogenesis compared with control medium, giving a response that was inhibited by VEGF-neutralizing antibody treatment. Inhibition of protein kinase C (PKC) prevented PTHrP-induced extracellular signal-regulated kinase (ERK1/2) and p38 activation, and PTHrP-induced VEGF expression. CONCLUSION:PTHrP plays an important role in modulating the angiogenic and bone osteolytic actions of VEGF through PKC-dependent activation of an ERK1/2 and p38 signaling pathway during bone metastasis by breast cancer cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Isowa S,Shimo T,Ibaragi S,Kurio N,Okui T,Matsubara K,Hassan NM,Kishimoto K,Sasaki Asubject
Has Abstractpub_date
2010-07-01 00:00:00pages
2755-67issue
7eissn
0250-7005issn
1791-7530pii
30/7/2755journal_volume
30pub_type
杂志文章abstract：:In procaryotic and also in eucaryotic cells, several systems for repairing the DNA damage have been discovered. The mechanism and genetic control of DNA excision-repair and SOS have been extensively studied. These repair processes help prevent lesions from interfering with DNA functions or from converting into permane...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1988-09-01 00:00:00
abstract：UNLABELLED:Backgroud: Helicobacter pylori (Hp) rarely proliferates in patients with fundic gland polyps (FGPs). We recently found that FGPs express lysozyme, one of the natural defence substances against infection. We aimed to assess the degree of lysozyme expression in a cohort of consecutive FGPs. MATERIALS AND METH...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Tyrosine kinase inhibitors (TKIs) directed against epidermal growth factor receptor (EGFR) are important in the treatment of non-small cell lung cancer (NSCLC), especially those harboring EGFR mutations. But little is known regarding the clinical value of serum tumor marker levels measured prior to treatment...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12018
更新日期：2017-10-01 00:00:00
abstract：:We have measured serum CEA levels in 207 patients with colorectal cancer. CEA sensitivity was related to tumor stage in tumors located in the right colon, but not in tumors located in the left colon or the rectum. CEA had prognostic value in patients with tumors located in the right colon (p < 0.001) but not in the le...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：BACKGROUND:Ano-genital carcinoma is a polyfactorial and polygenic disease. Certain strains of human papillomavirus (HPV) have been detected in a high percentage of patients. It has been suggested that p53 polymorphisms may be relevant for the interaction with viral proteins that inactivates p53. MATERIALS AND METHODS:...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Keratinocytes originating from adult human hair follicles, the most convenient biopsy tissue, can be serially cultured using a combination of two techniques. Primary cultures are established using plucked scalp hair follicles and the bovine eye lens capsule as a growth substrate. Subsequently, cells from these culture...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:This review summarizes our experiments which are investigating the relationship between the structure and activity of mainly phenothiazines, benzo[a]phenothiazines and benz[c]acridines. Phenothiazines had potent antiplasmid and antibacterial activities, but induced weak antimicrobial activity in vivo. Their antiplasmi...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1993-07-01 00:00:00
abstract：:We examined 11 patients (9 male and 2 female, age 41 +/- 13) presenting astrocytomas of different malignancy grade (WHO) with single voxel (PRESS) and double voxel (STABLE) spectroscopy techniques. The content of choline in the tumors was determined as a function of their grade of malignity and a significant correlati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND/AIM:Identifying useful biomarkers is central to selecting optimal therapeutic strategies for esophageal squamous cell carcinoma (ESCC). Serum p53 antibody (S-p53Ab), squamous cell carcinoma antigen (SCC-Ag), and carcinoembryonic antigen (CEA) were investigated to evaluate the significance of single and combi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12419
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND:Oxi4503 has been shown to inhibit tumor growth and improve survival in an animal model of colorectal (CRC) liver metastases. This agent appears to selectively target the endothelial cytoskeleton with resultant vessel occlusion and tumor necrosis. MATERIALS AND METHODS:This study evaluated the pattern of tum...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:The karyotypes and selected isoenzymes of five cell lines, HU 961b, HU 1922, HU 609, HCV-29 and HU 1703 He, were characterized. These cell lines were derived either from histologically normal human urothelial cells or from transitional cell carcinoma (TCC) of the urinary bladder. They displayed different life span in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:The development of cisplatin drug resistance remains a chief concern in ovarian cancer chemotherapy. β-Elemene is a natural plant product with broad-spectrum antitumor activity towards many types of carcinomas. This study aimed to define the biological and therapeutic significance of β-elemene in chemoresistant ovaria...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：AIM:The present study investigated the clinical significance of tartrate-resistant acid phosphatase type-5 (ACP5) expression in gastric cancer. MATERIALS AND METHODS:In 150 specimens of gastric cancer and adjacent normal mucosa, expression of ACP5 protein and mRNA and was determined by immunohistochemical staining and...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that ar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Bcl-2 oncoprotein, a member of a new category of oncogenes associated with the regulation of programmed cell death (apoptosis), has been considered to be involved in biological processes such as tumorigenesis and tumor development. To determine the role of bcl-2 oncoprotein in lung cancer, we preliminarily examined th...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Molecular chaperones, many of which are heat-shock proteins (HSPs), are an important class of molecules with various functions. Pathological conditions in which chaperones become etiological and/or pathogenic factors are called chaperonopathies, and are classified into by defect, by excess, and by 'mistake'. In the la...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2012-12-01 00:00:00
abstract：:The development of multidrug resistance (MDR) causes difficulties in the chemotherapy of of human cancer. Investigation of the possibility of reversal of MDR has been greatly aided by the use of cell lines with acquired resitance to anticancer agents in vitro or transfected with the mdrl gene. The aim of this study wa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:We studied a combined utilization of serum tumour markers and proton nuclear magnetic resonance 1H NMR spectroscopy of plasma in the detection of pancreatic cancer. Serum concentrations of carcinoembryonic antigen (CEA), carboanhydrate antigens CA 50 and CA 242, and 1H NMR spectra of plasma were determined in a series...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:Metastatic renal cell carcinoma (RCC) has a poor response to anticancer chemotherapy and radiotherapy. While immunotherapy and molecular targeted drugs have been used as first-line therapy for RCC metastasis, the response rate to these agents is low. We report the case of a patient with lung and bone metastases of RCC...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：:In order to improve the treatment of advanced H&N cancer we must consider to adopt new strategies as: new/better cytostatic agents; new combinations of present cytostatic agents and; potentiation of radiotherapy and cytostatic agents by biochemical modulation, which we define as potentiation of therapy by non-chemothe...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND/AIM:To investigate the impact of inguinal lymph node dissection (ILND) following neoadjuvant chemoradiotherapy (NACRT) for rectal cancer patients with ILN metastasis. PATIENTS AND METHODS:Forty-three patients with rectal cancer underwent NACRT followed by curative surgery between January 2005 and December 2...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13779
更新日期：2019-10-01 00:00:00
abstract：:The effects of the oral administration of 100 mg/kg/day egg-white lysozyme (EWL) on the expression of CD3, CD4, CD8 and CD25 antigens of lymphocytes harvested from IEL and mesenteric lymph nodes (MLNL) were tested in mice bearing MCa mammary carcinoma. Lysozyme, after oral administration, retains its enzymatic activit...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Using reverse transcriptase polymerase chain reaction amplification it was possible to detect the presence of oestrogen sulphatase mRNA in different hormone-dependent (MCF-7, T-47D) and hormone-independent (MDA-MB-231, MDA-MB-468) mammary cancer cell lines. The expression of this mRNA is significantly higher in T-47D ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:The complication of metastasis from the primary tumor site to the distant organ is difficult to control. Tumor cells usually spread through the vascular system: lymphatics and/or blood; or by direct extension into adjacent sites. Transplantable prostate adenocarcinoma (PA-III) cells in L-W rats produces a tumor in the...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:There is sparse information about cancer in the axillary tail of Spence (CATS). Eight hundred and thirty-nine patients with breast cancer were retrospectively studied for the occurrence of CATS. Ten patients were identified based on detection by imaging studies. A tendency towards stage II or III disease, and estrogen...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:The carcinogen Fe-NTA catalyzes the hydrogen peroxide-derived production of free radicals and possibly acts through a mechanism involving oxidative stress. Fermented papaya preparation (FPP) has been reported as a natural antioxidant able to prevent lipid peroxidation in vitro and in vivo. However, little is known abo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：BACKGROUND/AIM:Locoregional recurrence (LRR) of breast cancer is reported to occur at a rate of 5%-15%. Wide excision of LRR is the recommended treatment, which can increase the probability of subsequent local control. Herein, we describe a surgical technique wherein a pedicled skin and subcutaneous flap close the skin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14589
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND/AIM:Biosimilar agents are biologic products that have no clinically meaningful differences in terms of quality, efficacy, safety and immunogenicity compared to an already approved reference biological product, with the potential to reduce the costs of biologics. Considering the increasing numbers of oncology...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13552
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND/AIM:Lymphatic invasion (ly) may mainly reflect the selective affinity of breast cancer cells for lymph nodes. We conducted the present study to investigate whether the presence of lymphatic invasion is a predictor of sentinel lymph node (SLN) metastasis in clinically node-negative breast cancer. PATIENTS AN...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND/AIM:Advanced anaplastic thyroid cancer (ATC) is a rare, but highly aggressive malignancy, and its prognostic factors need to be further explored. We examined socioeconomic factors' predictive effect for survival performance in stage IV ATC patients. MATERIALS AND METHODS:Using the Surveillance, Epidemiology...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13198
更新日期：2019-02-01 00:00:00
