Abstract:
:It has been reported that artificial restoration of barrier function by a water vapor-impermeable membrane after tape stripping induces barrier abrogation in hairless mice, impeding rather than enhancing barrier recovery. To address this issue, we examined the morphologic changes in the epidermis after tape stripping and occlusion with a water vapor-impermeable membrane in murine skin. Male hairless mice were used for all studies of barrier perturbation and occlusion. Barrier disruption was achieved by repeated application of cellophane tape. Immediately after tape stripping the animals were wrapped in a tightly fitting water vapor-impermeable membrane. Transepidermal water loss (TEWL) was measured 20 min after tape stripping and 14, 24, 36, 48 and 60 h after occlusion. For electron microscopy the samples were treated with osmium tetroxide (OsO4) or ruthenium tetroxide (RuO4). When tape-stripped animals were wrapped in a water vapor-impermeable membrane, thereby preventing water flux, barrier function did not recover normally. These results demonstrate that an artificial block to TEWL with an impermeable membrane did not enhance barrier recovery. By electron microscopy many transitional cells and lacunae of various sizes were seen within the intercellular spaces of the stratum corneum after occlusion following tape stripping. Occlusion also caused alterations in both lipid lamellar membrane structures in the stratum corneum interstices and the lamellar bodies in the cytosol of granulocytes and transitional cells. Secreted lamellar body contents also appeared to be abnormal in the stratum corneum-stratum granulosum junction.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Jiang S,Koo SW,Lee SHdoi
10.1007/s004030050280subject
Has Abstractpub_date
1998-03-01 00:00:00pages
145-51issue
3eissn
0340-3696issn
1432-069Xjournal_volume
290pub_type
杂志文章abstract::The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) on type-IV-procollagen (basement-membrane collagen) and laminin synthesis, turnover, and secretion was studied in human A431 squamous epidermoid carcinoma cells. Type-IV procollagen and laminin were biochemically and immunologic...
journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00374074
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 历史文章,杂志文章
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00403844
更新日期:1980-01-01 00:00:00
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509062
更新日期:1982-01-01 00:00:00
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