Abstract:
AIM:To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS:737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested for the presence of C difficile toxin by EIA for toxin A and by cytotoxicity assay. Clinical data were evaluated in all cases positive by either method. RESULTS:With the cytotoxicity assay as a gold standard, the specificity of EIA for toxin detection was 99.3% and the sensitivity was 62.2%. No false negative EIA specimens were obtained from patients already being treated for C difficile colitis. Among patients with cytotoxicity positive specimens, those with EIA positive samples had no clinical features distinguishing them from patients with EIA negative samples. CONCLUSIONS:Although specific, the new EIA method directed against toxin A lacks sensitivity compared to cytotoxicity. False negative EIA tests are not associated with concurrent treatment for C difficile colitis nor with any specific clinical features examined in our study.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Vargas SO,Horensky D,Onderdonk ABdoi
10.1136/jcp.50.12.996subject
Has Abstractpub_date
1997-12-01 00:00:00pages
996-1000issue
12eissn
0021-9746issn
1472-4146journal_volume
50pub_type
杂志文章abstract:BACKGROUND/AIMS:The programmed cell death receptor 1 (PD-1) checkpoint inhibitor, nivolumab, has been approved for the treatment of metastatic renal cell carcinoma (RCC). However, the understanding of the expression and distribution of PD ligand 1 (PD-L1) in the tumour immune microenvironment and its prognostic role in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206092
更新日期:2020-08-01 00:00:00
abstract::Tumour associated antigens recognised by cellular or humoral effectors of the immune system are potential targets for antigen specific cancer immunotherapy. Different categories of cancer antigens have been identified that induce cytotoxic T lymphocyte (CTL) responses in vitro and in vivo, namely: (1) "cancer testis" ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.9.669
更新日期:2001-09-01 00:00:00
abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.040782
更新日期:2007-09-01 00:00:00
abstract:AIM:To develop a unified diagnostic approach for the detection of human papillomavirus (HPV) DNA in skin and mucosal biopsies from both immunocompetent and immunosuppressed individuals using a degenerate polymerase chain reaction (PCR) method. METHODS:The sensitivity and specificity of three published degenerate prime...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.8.606
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND:Gastric mucosa associated lymphoid tissue (MALT) lymphoma is a low grade B cell lymphoma histologically characterised by neoplastic B cells surrounding follicles in a marginal zone pattern and selectively infiltrating epithelium to form characteristic lymphoepithelial lesions. AIMS:To identify solitary epit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.023747
更新日期:2005-11-01 00:00:00
abstract:AIMS:To investigate the expression of the placental cell-specific associated proteins in infantile haemangioma (IH). METHODS:Immunohistochemical staining was used to investigate the expression of human chorionic gonadotrophin (hCG), human placental lactogen (hPL), human leucocyte antigen-G (HLA-G), cytokeratin 7 (CK7)...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200191
更新日期:2011-10-01 00:00:00
abstract::The sensitivity patterns to a number of antibiotic-impregnated discs of 126 strains of Gram-negative anaerobes and 36 strains of Gram-positive non-sporing anaerobes from various sources have been examined. The sensitivity patterns shown by Bacteroids fragilis and Bacteroides melaninogenicus were useful for the rapid i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.5.388
更新日期:1975-05-01 00:00:00
abstract::Targeted next-generation sequencing using the 50-gene Ion AmpliSeq Cancer Hotspot Panel v2 identified two significant point mutations in endometrial stromal sarcomas (ESS). Case 1 is a uterine mass from a quadragenarian woman with a karyotype lacking any known ESS rearrangements but demonstrated to have a CTNNB1-activ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206432
更新日期:2020-12-01 00:00:00
abstract:AIMS:The IHC4+C score combines assessment of oestrogen receptor (ER), progesterone receptor (PgR), HER2 and Ki67 with clinicopathological parameters to identify the risk of distant disease recurrence in patients with breast cancer, so, aiding treatment decision-making on adjuvant chemotherapy. Despite low cost and wide...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2015-203212
更新日期:2016-02-01 00:00:00
abstract:AIM:Nowadays, extracellular vesicles are of great interest in prostate cancer (PCa) research. Asparagine (N)-linked glycosylation could play a significant role in the pathological mechanism of these vesicles. We investigated if prostatic protein N-glycosylation profiles were related to urinary vesicle-associated prosta...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204312
更新日期:2017-10-01 00:00:00
abstract::Malignant histiocytosis (histiocytic medullary reticulosis) in a 45-year-old white man is described. Unusual features were presentation as a surgical emergency with signs of obstruction and peritonitis due to an ileal tumour and extensive spindle cell differentiation. Problems in the differential diagnosis of malignan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.2.120
更新日期:1977-02-01 00:00:00
abstract:AIMS:Diffuse large B-cell lymphoma (DLBCL) is an aggressive and potentially fatal disease. Prediction of risk of relapse is based on clinical markers. There is a need for more accurate biomarkers to select patients for more aggressive first-line treatments. Peroxiredoxins (Prxs) are a family of potent antioxidant prote...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202771
更新日期:2015-07-01 00:00:00
abstract::Cancer lineage/tissue-of-origin assignment in cancers of unknown primary remains a challenge even when aided by massively parallel sequencing. The stakes are high for patients as many contemporary therapeutic strategies are disease-specific, and the biological differences can influence the patients' responses. Herein,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206895
更新日期:2020-08-27 00:00:00
abstract:AIMS/BACKGROUND:A major medical role for postmortem examinations is the detection of clinically unexpected disease processes contributing to death. The aim of the present study was to determine whether simple clinical parameters can predict the presence of important unanticipated findings at necropsy. METHODS:Prospect...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.11.909
更新日期:1996-11-01 00:00:00
abstract::Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). Ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.6.475
更新日期:1999-06-01 00:00:00
abstract::The stability of the commonly used red cell radio labels chromium-51, indium-(111 or 113m), and technetium-99m, within intact red cells and stroma and their distribution within the cell were compared in undamaged and heat damaged red cells in relation to the clinical use of heat damaged cells in the assessment of sple...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.7.717
更新日期:1986-07-01 00:00:00
abstract::Serum lysozyme levels were determined by a turbidometric method using egg white lysozyme as standard in 100 patients with Crohn's disease, 86 with ulcerative colitis, 31 with coeliac disease, and in 38 normal control subjects. Though the levels in Crohn's disease were significantly higher than those in ulcerative coli...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.7.598
更新日期:1976-07-01 00:00:00
abstract:AIMS:To develop a quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) for severe acute respiratory syndrome coronavirus (SARS-CoV) detection and explore the potential of using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control to exclude false negative results. METHODS:SAR...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.016592
更新日期:2005-03-01 00:00:00
abstract::The microwave tissue coagulator (MTC) is used in hepatectomy because it provides excellent haemostasis during the procedure. A 59 year old man underwent partial hepatic lobectomy with MTC, for metastasis from colon cancer. A tumorous necrotic nodule was discovered in the liver. The nodule measured 2.5 cm at its larges...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.8.703
更新日期:1997-08-01 00:00:00
abstract::Although the UK National Institute for Health and Care Excellence guidelines recommend that in patients with biopsy-proven invasive lobular carcinoma (ILC), preoperative MRI scan is considered, the accuracy of diagnosis of ILC in core biopsy of the breast has not been previously investigated. Eleven pathology laborato...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2016-203886
更新日期:2016-12-01 00:00:00
abstract::Tumour tissue from 29 patients with primary brain lymphoma was reviewed to determine if there was an aetiological association between Epstein-Barr virus and polyclonal and monoclonal lymphoproliferations. The morphology and immunophenotype in 24 patients for whom paraffin wax embedded tissue was available were studied...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.3.220
更新日期:1990-03-01 00:00:00
abstract::The neutrophil shape change response to a chemotactic formylpeptide was assessed. Neutrophil bipolar shape formation (BSF) was also simultaneously assessed with a Boyden chamber-based neutrophil migration assay. Both assays were precise and relatively reproducible; the average coefficient of variation for the BSF assa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.4.342
更新日期:1990-04-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract::Prostaglandin E(2) (PGE(2)) was infused intravenously to eight women for the termination of pregnancy and tests of platelet function: coagulation and fibrinolysis were studied before and during the infusion.Platelet adhesiveness, as measured by a cellophane membrane test-cell system, was significantly diminished by PG...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.5.354
更新日期:1973-05-01 00:00:00
abstract:BACKGROUND:The terminology applied to vascular anomalies has been variable in previously published literature making interpretation suboptimal. The International Society for the Study of Vascular Anomalies (ISSVA) has proposed a revised classification based on clinical features and histopathological findings. This clas...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038240
更新日期:2006-12-01 00:00:00
abstract::The incidence of placenta accreta, defined as the abnormal adherence of the placenta to the uterine wall, has been increasing alarmingly in the developed as well as the developing world. There is considerable maternal morbidity and mortality related to the condition. The pathophysiology focuses on the balance between ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2008.055202
更新日期:2008-12-01 00:00:00
abstract::Meningitis due to Gemella haemolysans developed in a 73 year old woman after thermolysis of the Gasserian ganglion for trigeminal neuralgia. The taxonomy of this organism is discussed, and previous cases of infection are reviewed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.5.558
更新日期:1985-05-01 00:00:00
abstract::Protein HC and protein HC-IgA complex were measured in 18 different types of fluid sample from healthy subjects and patients with different illnesses to determine if the concentrations of protein HC and protein HC-IgA complexes could be used to monitor certain diseases, when measured separately. The normal values for ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.11.1176
更新日期:1988-11-01 00:00:00
abstract::A study has been made by clinical methods and by bio-assay of the effectiveness of a régime for prophylaxis against tetanus. The two principal objects of the method were to ensure that active immunization was offered to all patients who had received antitoxin and to measure the effect of the combined administration of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.1.23
更新日期:1965-01-01 00:00:00
abstract::In this necropsy review of 37 cases of acute pancreatitis, evidence is presented that there are at least two different initiating mechanisms in acute necrotising pancreatitis in man. Firstly, there is primary duct inflammation, with subsequent inflammation and necrosis of the pancreatic parenchyma surrounding the excr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.12.1125
更新日期:1980-12-01 00:00:00