Abstract:
AIMS:To develop a quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) for severe acute respiratory syndrome coronavirus (SARS-CoV) detection and explore the potential of using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control to exclude false negative results. METHODS:SARS-CoV and GAPDH mRNA were both measured in 26 specimens from 16 patients with SARS, 40 follow up specimens from the same batch of patients, and appropriate control subjects. The relation between SARS positivity and GAPDH mRNA concentration was investigated using the chi2 test. Increasing the sensitivity for SARS-CoV and GAPDH mRNA detection was investigated in follow up specimens in which SARS-CoV and GAPDH mRNA were not detected initially. RESULTS:Varying amounts of SARS-CoV were found in the 26 SARS-CoV positive specimens and SARS-CoV was not detected in the 40 follow up specimens and controls. In addition, concentrations of GAPDH mRNA were significantly different between the patients with SARS, follow up specimens, and healthy controls (Kruskal-Wallis test, p<0.05). Moreover, GAPDH mRNA concentrations were highly correlated with SARS-CoV positivity (chi2 = 5.43; p<0.05). Finally, SARS-CoV and GAPDH mRNA were both detected in three follow up urine specimens that were initially negative when the amount of cDNA used was increased from 5 microl to 10 and 15 microl. CONCLUSIONS:This Q-RT-PCR assay can be used to detect SARS-CoV. Moreover, GAPDH mRNA may be useful to rule out false negative results in SARS-CoV detection, and the current extraction method for urine may not be sensitive enough to detect low titres of SARS-CoV.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Wong SC,Chan JK,Lee KC,Lo ES,Tsang DNdoi
10.1136/jcp.2004.016592keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
276-80issue
3eissn
0021-9746issn
1472-4146pii
58/3/276journal_volume
58pub_type
杂志文章abstract::Mixed lymphocyte reactions (MLRs) were measured in 25 HLA-A, B and DR compatible sibling bone marrow transplants. Only four of 25 MLRs were positive and in these the low reactivity was of doubtful clinical importance. There was no correlation between MLR and the subsequent development or severity of graft versus host ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.11.1155
更新日期:1988-11-01 00:00:00
abstract::Human oxalated plasma stored at 4 degrees C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreem...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.6.457
更新日期:1960-11-01 00:00:00
abstract:AIMS:To analyse the expression of proteins involved in DNA double strand break detection and repair in the luminal and myoepithelial compartments of benign breast lesions and malignant breast tumours with myoepithelial differentiation. METHODS:Expression of the ataxia telangiectasia (ATM) and p53 proteins was immunohi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.017434
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abstract::The microwave tissue coagulator (MTC) is used in hepatectomy because it provides excellent haemostasis during the procedure. A 59 year old man underwent partial hepatic lobectomy with MTC, for metastasis from colon cancer. A tumorous necrotic nodule was discovered in the liver. The nodule measured 2.5 cm at its larges...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.8.703
更新日期:1997-08-01 00:00:00
abstract:AIM:To determine whether the pattern of involvement of the gastric mucosa in lymphocytic gastritis is predictive of the presence or absence of duodenal pathology. METHODS:50 cases (M:F, 26:24; median age 57 years) diagnosed as lymphocytic gastritis between 1986 and 1998 with concurrent duodenal (D2) biopsies were iden...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.11.815
更新日期:1999-11-01 00:00:00
abstract::Toxoplasmosis is an infection, congenital or acquired, caused by Toxoplasma gondii. A pilot study using toxoplasmin skin antigen was undertaken to establish the incidence of the infection in the London area as a preliminary to a larger study of the effects of the infection upon pregnancy, the foetus, and the newborn i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.6.649
更新日期:1969-11-01 00:00:00
abstract::The T(4)-free thyroxine index was used as the thyroid function test of first choice in a district general hospital for one year. Ninety-two patients were assessed both initially and at follow up by a single physician. The index agreed with clinical assessment in 69 out of 74 patients in whom diagnosis was unequivocal....
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.3.259
更新日期:1972-03-01 00:00:00
abstract::The excretion of 2,3-dinor-6-keto prostaglandin F1 alpha, a major urinary metabolite of prostacyclin, and the formation of thromboxane B2, a stable metabolite of thromboxane A2, by platelets stimulated by adenosine diphosphate, were studied in alcoholics, who had been admitted for detoxification. Once prolonged heavy ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.5.512
更新日期:1987-05-01 00:00:00
abstract::A case of pigmented basal cell carcinoma developing in a smallpox revaccination site is presented. Any progressive change within a smallpox vaccination scar should be thoroughly evaluated and treated appropriately after tissue diagnosis. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.2.134
更新日期:1980-02-01 00:00:00
abstract::Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting grow...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201234
更新日期:2013-04-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.8.742
更新日期:1993-08-01 00:00:00
abstract::Superficial inflammatory dermatoses are very common and comprise a wide, complex variety of clinical conditions. Accurate histological diagnosis, although it can sometimes be difficult to establish, is essential for clinical management. Knowledge of the microanatomy of the skin is important to recognise the variable h...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2005.027151
更新日期:2005-12-01 00:00:00
abstract:AIMS:Pim-1 is a serine/threonine kinase that has been shown to play an integral role in the development of a number of human cancers, such as haematolymphoid malignancies. Recently, evidence has shown Pim-1 to be important in prostatic carcinogenesis. In order to further our understanding of its role in prostate cancer...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.027672
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVES:To determine whether antibiotics are prescribed appropriately for acute tonsillitis in an emergency department (ED). METHODS:Cross-sectional observational study in large district general hospital in London. Patients diagnosed and coded with 'acute tonsillitis' in the ED over a 3-month period in 2015. Medica...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203808
更新日期:2016-09-01 00:00:00
abstract::A 65-year-old woman with a history of a left heminephrectomy for renal carcinoma developed hypercalcaemia 11 years after the operation. The same kidney was found to contain a recurrent renal carcinoma. After the radical nephrectomy of the left kidney, hypercalcaemia remitted but reappeared 11 months later. The right k...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.3.234
更新日期:1979-03-01 00:00:00
abstract::One hundred sera from 49 patients with glandular toxoplasmosis were examined by a latex agglutination test, the dye test, an indirect haemagglutination test, and a double antibody sandwich enzyme linked immunosorbent assay (ELISA) for antitoxoplasma IgM. The results support previous findings that the dye test, indirec...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.11.1293
更新日期:1984-11-01 00:00:00
abstract::An immunochemical procedure for routine laboratory diagnosis of pregnancy utilizing adsorption of human chorionic gonadotrophin onto kaolin and the Ortho pregnancy test reagents is described. The method is suitable for quantitative assay of urinary human chorionic gonadotrophin and can be adapted for use with serum. I...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.5.548
更新日期:1964-09-01 00:00:00
abstract:BACKGROUND:The TNM classification for renal cell cancer (RCC) should accurately predict and assign prognostic information for patients. In this study the recent 2010 revision to the TNM classification was compared with the previous 2002 classification with regard to survival outcomes. METHODS:All patients having radic...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200468
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:During the last decade, whole slide images (WSI) have been used in many areas of pathology such as teaching, research, digital archiving, teleconsultation and quality assurance testing. However, WSI have not regularly been used for routine diagnosis, because of the lack of validation studies. AIM:To test th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200277
更新日期:2012-02-01 00:00:00
abstract::Serum total calcium was measured in 1693 patients during a four-month period. We examined the effects of adjustment for albumin concentration on the interpretation of single measurements of serum total calcium and on the variation of series of measurements in individual patients. Markedly abnormal total calcium concen...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.1.56
更新日期:1979-01-01 00:00:00
abstract::Routine endometrial cytology was used instead of curettage as the first step in a morphological investigation of the endometrium in patients with postmenopausal bleeding. Premenopausal women with symptoms or signs indicative of premalignant or malignant disease were also studied using this method. Patients with alarmi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.1.36
更新日期:1986-01-01 00:00:00
abstract::Candida spp are the fourth most common group of nosocomial pathogens isolated from patients on medical, surgical, and intensive care wards. Polymicrobial candidaemia has rarely been described. The diagnosis of candidaemia from peripheral blood smears has not been widely reported. This report describes the case of a yo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2003.9340
更新日期:2004-02-01 00:00:00
abstract::Blood cultures drawn from a patient with clinically diagnosed invasive meningococcal disease, who had been previously administered benzylpenicillin, had beta-lactamases added to increase the probability of recovery of the causative organism. The blood cultures subsequently yielded Neisseria meningitidis but direct sus...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.11.893
更新日期:2001-11-01 00:00:00
abstract::A method for the neutron activation analysis of copper in biological material is described and applied to the diagnosis and management of four cases of Wilson's disease. Results obtained for serum and urine are in agreement with values obtained by established colorimetric techniques. The method described can estimate ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.1.8
更新日期:1968-01-01 00:00:00
abstract::Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.185
更新日期:1967-03-01 00:00:00
abstract::A simple, standardised procedure was used for processing data from External Quality Assessment Schemes and for producing performance charts. A graphic presentation of cumulative performance is generated for each test, using an integrated software package to display results in a standardised format. This permits interp...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.1.17
更新日期:1991-01-01 00:00:00
abstract::The stability of the commonly used red cell radio labels chromium-51, indium-(111 or 113m), and technetium-99m, within intact red cells and stroma and their distribution within the cell were compared in undamaged and heat damaged red cells in relation to the clinical use of heat damaged cells in the assessment of sple...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.7.717
更新日期:1986-07-01 00:00:00
abstract::A patient with multiple myeloma had antigenically related monoclonal Fc-gamma fragments and complete IgG-kappa molecules in the serum. The urine contained only Fc-gamma fragments in the absence of Bence-Jones protein. The two distinct M-components in the serum showed electrophoretic identity but could be separated by ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.10.936
更新日期:1980-10-01 00:00:00
abstract::The peripheral lymphocytes of 50 cases of human immunodeficiency virus (HIV) infection (13 of acquired immune deficiency syndrome (AIDS), 17 of AIDS related complex (ARC), and 20 healthy carriers) were studied immunoultrastructurally. The prevalence of "tubuloreticular structures" and "tubular confronting cisternae" i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.1.62
更新日期:1988-01-01 00:00:00
abstract:AIMS:To study the overexpression of p53 protein in Barrett's oesophagus with adenocarcinoma, and to correlate this expression with the pathological features of Barrett's syndrome. METHODS:Immunohistochemical staining was performed on frozen sections with a monoclonal antibody directed against wild type and mutated p53...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.4.330
更新日期:1993-04-01 00:00:00