Abstract：
AIMS:To investigate treatment with glycerol/washing as a potential substitute for freeze-thawing in the production of leucocyte depleted red cell concentrates for patients with a history of non-haemolytic reactions following transfusion. METHODS:The standard procedure of treatment with glycerol/-80 degrees C freezing/thawing/washing was compared with a similar procedure in which freezing was omitted. The quality of the resulting red cell products was assessed in relation to: (1) standard red cell biochemical parameters; (2) leucocyte and lymphocyte subset composition using flow cytometry with fluorescent labelled monoclonal antibodies; and (3) immunogenicity of the residual lymphocytes in mixed lymphocyte culture. RESULTS:Compared with red cells subjected to the standard freeze-thaw technique, red cells undergoing the non-freezing procedure and suspended in additive solutions had significantly better biochemical preservation after 21 days of storage (p < 0.001). Both procedures removed an average 98% of the initial leucocytes at the expense of 18-20% of the red cells. The non-freezing procedure resulted in higher residual concentrations of HLA class II bearing lymphocytes (p < 0.01), but not higher numbers of dendritic cells. Both procedures were equally effective in annulling the residual lymphocytes' ability to act as stimulator cells in one-way mixed lymphocyte culture. CONCLUSIONS:The non-freezing procedure produces a superior product for the provision of red cells to patients with granulocyte antibodies. These products may also offer a lower risk of HLA alloimmunisation to previously unexposed patients.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Farrugia A,Shea N,Knowles S,Holdsworth R,Piouronowski H,Portbury D,Romeo Adoi
10.1136/jcp.46.8.742subject
Has Abstractpub_date
1993-08-01 00:00:00pages
742-5issue
8eissn
0021-9746issn
1472-4146journal_volume
46pub_type
杂志文章abstract：AIMS:To compare the expression of immunohistochemical variables between benign and malignant components of malignant peripheral nerve sheath tumour (MPNST) arising within neurofibroma. METHODS:Eight cases of MPNST arising within a neurofibroma, associated with neurofibromatosis type 1 (NF1), were studied. The areas of...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.54.8.631
更新日期：2001-08-01 00:00:00
abstract：AIMS:Computational pathology platforms incorporate digital microscopy with sophisticated image analysis to permit rapid, continuous measurement of protein expression. We compared two computational pathology platforms on their measurement of breast tumour oestrogen receptor (ER) and progesterone receptor (PR) expression...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2016-204107
更新日期：2017-05-01 00:00:00
abstract：:Cytomegalovirus (CMV) is a recognised cause of morbidity and mortality in immunocompromised individuals. This review will concentrate on recent advances in the understanding of the complex interplay between the host and parasite and the pathological consequences of perturbation of the host immune system. The classic v...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.54.2.84
更新日期：2001-02-01 00:00:00
abstract：:Pre-mRNA splicing is an essential process in eukaryotic cells where the transcribed intronic sequences are removed, prior to translation into protein. PRPF31 is a ubiquitously expressed splicing factor, which aids in the assembly of the macromolecular spliceosome. Mutations in PRPF31 cause autosomal dominant retinitis...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jclinpath-2016-203971
更新日期：2017-09-01 00:00:00
abstract：:Electron microscopical and cytochemical studies of intestinal biopsies from a patient with typical features of the Cronkhite-Canada syndrome show that the primary process affects the crypts. This results in cystic dilatation associated with expansion and focal degeneration of the crypt compartment of the intestinal ep...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.38.3.271
更新日期：1985-03-01 00:00:00
abstract：:A "Centrifichem" centrifugal analyser was found using a double fluorescence/bacterial tracer method to be a source of airborne and surface contamination in the laboratory. The airborne contamination was controlled by a modification incorporating a filter. Ubiquitous surface contamination generally accompanied the proc...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.41.11.1229
更新日期：1988-11-01 00:00:00
abstract：AIMS:To investigate the association of cagA positivity and non-opsonic neutrophil activation capacity in wild-type Helicobacter pylori strains with peptic ulcer disease or chronic gastritis only. METHODS:Helicobacter pylori were isolated from antral biopsies of 53 consecutive patients with chronic antral gastritis, of...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.53.4.318
更新日期：2000-04-01 00:00:00
abstract：:Bone marrow aspirates and biopsy specimens were taken at diagnosis from 51 patients with acute myeloid leukaemia (AML). The diagnosis was based on morphological and cytochemical analyses, and the leukaemias were classified by FAB criteria. A considerable difference was observed between the results of bone marrow aspir...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.42.3.300
更新日期：1989-03-01 00:00:00
abstract：:Specimens from 95 patients attending a venereal diseases clinic were examined for gonococci by three methods--a conventional culture technique using modified Thayer-Martin medium, microscopy of a Gram-stained direct smear, and the Microcult system. For 56% of the specimens the results by all three methods agreed. Assu...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.3.209
更新日期：1978-03-01 00:00:00
abstract：:An imprint culture technique has been employed to study the prevalence and intraoral distribution of Candida albicans in 16 patients with Sjögren's syndrome and in 16 healthy controls matched for age, sex, and dental status. The prevalence and intraoral density of C. albicans was found to be significantly higher at al...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.33.3.282
更新日期：1980-03-01 00:00:00
abstract：AIMS:To describe and evaluate two apparently unique cases of inverted hyperplastic (metaplastic) polyposis of the colon. METHODS:The cases were analysed by standard histopathological, histochemical, and immunohistochemical techniques and the findings compared with those of regular hyperplastic polyps of the colorectum...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.1.56
更新日期：1993-01-01 00:00:00
abstract：AIMS:Determine whether a simple prewash step will provide adequate amounts of high-quality DNA from core needle biopsies for molecular sequencing studies. METHODS:The quantitative and qualitative metrics of DNA recovered from core needle biopsies processed either by 1) formalin fixation and paraffin embedding (FFPE), ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2016-204061
更新日期：2017-06-01 00:00:00
abstract：:Immunoreactive plasminogen activators were studied in tissue sections using a peroxidase method and monospecific antibodies to tissue plasminogen activator produced by a melanoma. Tissue plasminogen activator reactivity was found in skin melanomas and in endothelial and smooth muscle cells of arteries and veins. Vesse...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.38.2.140
更新日期：1985-02-01 00:00:00
abstract：:Laboratory studies on clotrimazole showed that it had marked activity in vitro against all the Candida spp. and Cryptococcus spp. tested, against almost all strains of dermatophytes, and against Aspergillus spp and other fungal genera responsible for systemic mycoses; it had limited activity towards Gram-positive bact...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.25.12.1089
更新日期：1972-12-01 00:00:00
abstract：:This review focuses on the pathological features of dysplasia in Barrett's oesophagus. Two categorisation schemes are used for grading dysplasia in the gastrointestinal tract, including Barrett's oesophagus. The inflammatory bowel disease dysplasia morphology study group system is the one most commonly used in the USA...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2005.035337
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVE:To describe practical experiences in the sharing of very large digital data bases of histopathological imagery via the Internet, by investigators working in Europe, North America, and South America. MATERIALS:Experiences derived from medium power (sampling density 2.4 pixels/microm) and high power (6 pixels/...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.55.6.452
更新日期：2002-06-01 00:00:00
abstract：AIMS:The signalling system of phosphoinositides (PIs) is involved in a number of cell and tissue functions including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation and cell and tissue polarity. Recently, a role in cell migration was hypothesised for PI and related molecules including...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2011-200096
更新日期：2011-10-01 00:00:00
abstract：:The prognostic value of detection of human papillomavirus (HPV) type 16 DNA in histologically cancer free lymph nodes was assessed in left obturator lymph nodes from cervical cancer patients with HPV-16 positive primary tumours. HPV-16 DNA was detected by polymerase chain reaction in 12 of 35 patients with histologica...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.50.11.960
更新日期：1997-11-01 00:00:00
abstract：:Organising pneumonia (otherwise referred to as bronchiolitis obliterans organising pneumonia) is characterised histologically by plugs of granulation tissue, which are present predominantly within small airways, alveolar ducts and peri-bronchiolar alveoli. This pattern is not specific for any disorder or cause, but is...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jclinpath-2012-201342
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:The "classical" antineutrophil cytoplasmic antibody (C-ANCA) pattern seen on indirect immunofluorescence (IIF) is characterised by granular cytoplasmic staining showing central or interlobular accentuation, and is strongly associated with antiproteinase-3 antibodies (PR3-ANCA) and Wegener's granulomatosis. H...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.2.124
更新日期：1999-02-01 00:00:00
abstract：AIMS:Inflammatory bowel disease (IBD)-associated precancerous lesions may be adenomatous or non-adenomatous with various histomorphologies. We aim to validate the newly proposed classification, to explore the neoplastic nature of the non-adenomatous lesions and to elucidate the molecular mechanisms underlying the diffe...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2019-206247
更新日期：2020-07-01 00:00:00
abstract：:Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). Ther...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.6.475
更新日期：1999-06-01 00:00:00
abstract：:A rare case of adult onset Langerhans' cell histiocytosis associated with dysgerminoma in a 35 year old Chinese woman is reported. The patient had a history of dysgerminoma of left ovary 15 years previously and had undergone surgery followed by radiotherapy and an uneventful recovery. She presented again in March 1994...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.48.10.963
更新日期：1995-10-01 00:00:00
abstract：:The nasal carrier status of 3,736 patients was determined throughout their stay in hospital. The carrier rate on admission, which was highest in patients under 20 years of age, did not appear to vary with season.The carriage of strains resistant to penicillin increased with the patients' stay in hospital from 13.% on ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.17.1.79
更新日期：1964-01-01 00:00:00
abstract：AIM:To evaluate the accuracy of the approximate linear formula of International Normalised Ratio (INR) imprecision by formal mathematical analysis. METHODS:Using probability theory, an exact formula for the coefficient of variation (CV) of the INR was derived. The CV from the approximate formula was compared with the ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.7.635
更新日期：1994-07-01 00:00:00
abstract：:A miniaturised method for the identification of anaerobic bacteria is described which employs microtitre fermentation tests, a spot indole test and a nitrate reduction disc test. The results obtained are directly comparable with those produced by a standard conventional method in use at present. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.37.1.70
更新日期：1984-01-01 00:00:00
abstract：AIMS:To investigate the problems involved in undertaking immunohistochemistry (IHC) and nuclear morphometry using Bouin's fixed prostate biopsies. METHODS:Archival Bouin's fixed and formalin fixed, paraffin wax embedded prostatic biopsies were immunostained for three nuclear biomarkers (minichromosome maintenance prot...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2004.019299
更新日期：2005-03-01 00:00:00
abstract：:A pseudolymphomatous reaction involving subcutaneous tissue and underlying skeletal muscle is described in a woman who was receiving phenytoin sodium and phenobarbitone. The histological appearance was of nodular, densely cellular tissue composed of histiocytes, plasma cells, lymphocytes, fibroblasts, and occasional p...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.8.761
更新日期：1978-08-01 00:00:00
abstract：:Investigations into the immunological functions of three patients with chronic mucocutaneous candidiasis were carried out. Phagocytosis of Candida albicans, nitroblue tetrazolium dye reduction, complement, and immunoglobulin levels were normal. Candidacidal assays using the neutrophils of the patients in autologous se...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.33.4.370
更新日期：1980-04-01 00:00:00
abstract：:Performance of CHROMagar Acinetobacter was assessed for the selective isolation and identification of Acinetobacter baumannii. The medium was effective in suppressing the growth of other Gram-positive and Gram-negative species while the addition of KPC supplement ensured growth of only carbapenem resistant A baumannii...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2010.083469
更新日期：2011-02-01 00:00:00
