Abstract:
AIMS:The signalling system of phosphoinositides (PIs) is involved in a number of cell and tissue functions including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation and cell and tissue polarity. Recently, a role in cell migration was hypothesised for PI and related molecules including the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signalling. The expression of PI-PLCs is tissue-specific and evidence suggests that it varies under different conditions such as tumour progression or cell activation. In order to obtain a complete picture, the expression of all PI-PLC isoforms was analysed in human endothelial cells (EC). METHODS:Using molecular biology methods (RT-PCR), the expression of PI-PLC isoforms was analysed in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for human EC. RESULTS:All the PI-PLC isoforms except PI-PLC β1, PI-PLC ε and PI-PLC ζ were expressed in HUVEC. CONCLUSIONS:The growing interest in the complex cascade of events occurring in angiogenesis will provide useful insights for therapeutic strategies. The expression of PI-PLC isoforms in HUVEC is a useful tool for further studies directed to understanding their role in angiogenesis. However, although HUVEC represent a widely used experimental model for human macrovascular EC, limitations remain in that they cannot fully represent the metabolic properties and interactions of the EC distributed in the entire organism.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Lo Vasco VR,Pacini L,Di Raimo T,D'arcangelo D,Businaro Rdoi
10.1136/jclinpath-2011-200096subject
Has Abstractpub_date
2011-10-01 00:00:00pages
911-5issue
10eissn
0021-9746issn
1472-4146pii
jclinpath-2011-200096journal_volume
64pub_type
杂志文章abstract:AIMS:To establish whether abnormalities in the course of the vertebral artery occur and whether they are relevant to arterial injury associated with head and neck movements. METHODS:Twenty vertebral arteries were carefully dissected at necropsy and abnormalities in course were noted, along with any other bony or carti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.7.637
更新日期:1995-07-01 00:00:00
abstract:AIMS:To describe characteristics of a series of people accidentally and deliberately killed by air powered weapons. METHODS:Five cases of fatal airgun injury were identified by forensic pathologists and histopathologists. The circumstances surrounding the case, radiological examination, and pathological findings are d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.7.525
更新日期:1998-07-01 00:00:00
abstract::Organising pneumonia (otherwise referred to as bronchiolitis obliterans organising pneumonia) is characterised histologically by plugs of granulation tissue, which are present predominantly within small airways, alveolar ducts and peri-bronchiolar alveoli. This pattern is not specific for any disorder or cause, but is...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2012-201342
更新日期:2013-10-01 00:00:00
abstract::In a case of atypical mucin-producing bronchial carcinoid in a 40-year-old man covert metastases were present at the time of lobectomy and were revealed at necropsy--a feature highlighting the malignant potential of the atypical carcinoid. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1218
更新日期:1978-12-01 00:00:00
abstract:AIMS:Inflammatory bowel disease (IBD)-associated precancerous lesions may be adenomatous or non-adenomatous with various histomorphologies. We aim to validate the newly proposed classification, to explore the neoplastic nature of the non-adenomatous lesions and to elucidate the molecular mechanisms underlying the diffe...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206247
更新日期:2020-07-01 00:00:00
abstract::The sensitivity of 12 commonly used anti-HIV-1/HIV-2 enzyme immunoassays was evaluated. The assays, each of which utilises at least one synthetic HIV antigen, were tested against a panel of 1092 specimens previously designated anti-HIV positive. In a total of 13 104 tests there were eight false negative results attrib...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.5.490
更新日期:1995-05-01 00:00:00
abstract:AIMS:To determine the causes of death in patients with osteogenesis imperfecta, excluding infants with the perinatal lethal form (type II). METHODS:Seventy nine patients with known osteogenesis imperfecta were identified, 37 of whom had been seen clinically in life. Causes of death were identified from death certifica...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.8.627
更新日期:1996-08-01 00:00:00
abstract::Serum and tissue hepatitis B virus (HBV) markers were compared in 35 alcoholic and 23 non-alcoholic subjects affected by chronic liver disease. Seventeen point one per cent of alcoholic and 21.7% of non-alcoholic subjects had HBV tissue markers, but not serum markers, for this virus. It is therefore concluded that sho...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.2.167
更新日期:1987-02-01 00:00:00
abstract:AIMS:Optical microscopic (OM) evaluation of peripheral blood (PB) cells is still a crucial step of the laboratory haematological workflow. The morphological cell analysis is time-consuming and expensive and it requires skilled operator. To address these challenges, automated image-processing systems, as digital morphol...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206857
更新日期:2020-09-14 00:00:00
abstract:AIMS:Neoadjuvant chemoradiation therapy (CRT) is an increasingly used therapeutic strategy for rectal cancer. Clinically, it remains a major challenge to predict therapeutic response and patient outcome after CRT. Rsf-1 (HBXAP), a novel nuclear protein with histone chaperon function, mediates ATPase-dependent chromatin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-200786
更新日期:2012-08-01 00:00:00
abstract::In two families with severe sex-linked muscular dystrophy, high levels of alpha-hydroxybutyrate dehydrogenase (HBD), lactate dehydrogenase (LD), aspartate transaminase (AspT), aldolase, and creatine phosphokinase (CPK) were found in the sera of three young affected males. In both families the mother had a raised level...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.3.250
更新日期:1966-05-01 00:00:00
abstract::Splenic pathology was analysed in 73 patients with immune thrombocytopenic purpura who underwent splenectomy for bleeding that had been resistant to adrenocorticosteroids. The mean splenic weight was 100 g. The only notable macroscopic feature was the prominence of Malpighian corpuscles in 15 cases. Microscopic examin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.9.985
更新日期:1985-09-01 00:00:00
abstract::Review of published work and analysis of clinical data and pathology of four biopsy specimens from two patients with laryngeal contact granuloma showed that its peptic origin was derived from a gastro-oesophago-laryngeal reflux. It is proposed that the term "peptic granuloma" should be given to this phenomenon. This t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.8.800
更新日期:1989-08-01 00:00:00
abstract::This paper urges the benefits of applying more widely a method for pathology laboratory costing originally devised for a clinical chemistry department, and illustrates these with examples drawn from costing studies in three clinical laboratories. Heads of pathology departments, laboratory managers, administrators and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.9.1028
更新日期:1983-09-01 00:00:00
abstract:OBJECTIVE:Acute B lymphoblastic leukaemia (B-ALL) is the most common type of childhood malignancy worldwide but little is known of its origin. Recently, many studies showed both a high incidence of Epstein-Barr virus (EBV) infection and high levels of CD4+CD25+Foxp3+(Treg cells) in children with B-ALL. In our study, we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203803
更新日期:2017-02-01 00:00:00
abstract:AIMS:Common variable immunodeficiency (CVID) is the most common serious primary immunodeficiency. This paper describes the immunological consequences of human immunodeficiency virus (HIV) infection in a patient with familial CVID subsequently treated with highly active antiretroviral therapy (HAART). METHODS:Serial me...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.9.713
更新日期:2001-09-01 00:00:00
abstract::A rare case of adult onset Langerhans' cell histiocytosis associated with dysgerminoma in a 35 year old Chinese woman is reported. The patient had a history of dysgerminoma of left ovary 15 years previously and had undergone surgery followed by radiotherapy and an uneventful recovery. She presented again in March 1994...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.10.963
更新日期:1995-10-01 00:00:00
abstract::Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and high-throughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TM...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2012-201091
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Optimal management of patients with lung cancer requires accurate cell typing of tumours and staging at the time of diagnosis. Endobronchial ultrasound-guided lymph node aspiration as a method of diagnosing and staging lung cancer is a relatively new technique. AIM:To report the use of liquid-based-thin-lay...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038901
更新日期:2007-04-01 00:00:00
abstract::A study of 16 haematological and 24 biochemical parameters in healthy, adult, male, volunteer Saudi blood donors was made, and enabled a preliminary determination of the reference values for these parameters to be made for Saudi Arabia. Statistically significant low means were observed for all the haematological param...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.1.69
更新日期:1982-01-01 00:00:00
abstract::A 43-year-old man, admitted with a swollen right thigh, was diagnosed with a deep vein thrombosis. On day 6 he became septic. A CT scan demonstrated a right-sided hydronephrosis and a large retroperitoneal collection. Blood cultures and perinephric pus grew Escherichia coli resistant to amoxicillin only. On day 12 a v...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.071787
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:Despite advances in therapy, lung cancer is still the leading cause of cancer-related mortality in the world. Further prognostic tools are warranted for risk-adapted therapeutic decisions. We analysed a cohort of primary surgically treated non-small cell lung cancers (NSCLCs) to determine the prognostic role ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202819
更新日期:2015-05-01 00:00:00
abstract::Acute myocardial disease, which may be associated with abnormal platelet activity, has been reported after routine immunisation. Thirty-two army apprentices undergoing immunisation were studied for changes in the heparin neutralising activity (HNA) of platelet poor plasma. HNA increased after immunisation. This increa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1152
更新日期:1979-11-01 00:00:00
abstract::The applicability of a recently developed non-radioactive DNA labelling and detection method, which uses the digoxigenin (DIG) enzyme linked immunosorbent assay (ELISA) system, for the detection of viral infections in pathology specimens by in situ hybridisation, was examined. Its efficacy was compared with that of bi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.10.806
更新日期:1990-10-01 00:00:00
abstract::Serum labelled with (59)Fe for ferrokinetic studies should contain only [(59)Fe] transferrin. [(59)Fe] ferric citrate was incubated at 37 degrees C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [(59)Fe] ferric citrate may be present for several hours. An an...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.5.472
更新日期:1971-07-01 00:00:00
abstract::One hundred and twenty-one rectal biopsies from 99 patients with carcinoma of the rectum or lower sigmoid colon were investigated using a high iron-diamine-Alcian blue technique for sulphated and non-sulphated acid mucins. It was found that in normal rectal mucosa sulphomucins are the main carbohydrate component of th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.2.123
更新日期:1972-02-01 00:00:00
abstract::Sodium-alpha-naphthylamine-4-sulphonate (Naphthionin) appears to shorten the bleeding time in normal subjects and to be of value in the management of the bleeding defect in patients with thrombocytopenia when given by intravenous or intramuscular injection. A good response was achieved in three patients suffering from...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.6.680
更新日期:1964-11-01 00:00:00
abstract::Incomplete expression of human haemoglobin beta-chains at birth may lead to difficulty in the early demonstration of an inherited beta-chain variant. In this case, the rare unstable variant, Hb Tacoma beta30 (B12) arg leads to ser, although not present in cord blood in sufficient amounts to be easily detected by routi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.9.883
更新日期:1978-09-01 00:00:00
abstract:AIMS:Evidence suggests that the presence of tumour necrosis is an adverse prognostic factor in renal cell carcinoma (RCC). However, it has also been shown that tumour regression, a microscopic feature associated with necrosis, may be a favourable short term prognostic factor in RCC. METHODS:Pathology reports of 253 RC...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.018846
更新日期:2005-01-01 00:00:00
abstract::A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The mo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.6.628
更新日期:1984-06-01 00:00:00