Abstract:
:A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The morphology of the labelled cells is well preserved for analysis by light microscopy and the preparations obtained can be kept permanently. This method is useful for the analysis of mixed cell proliferations, particularly in leukaemias.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Tavares de Castro J,San Miguel JF,Soler J,Catovsky Ddoi
10.1136/jcp.37.6.628subject
Has Abstractpub_date
1984-06-01 00:00:00pages
628-32issue
6eissn
0021-9746issn
1472-4146journal_volume
37pub_type
杂志文章abstract::A case is described in which marked hepatic fibrosis probably resulted from the prolonged treatment of acute leukaemia with methotrexate. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.1.81
更新日期:1966-01-01 00:00:00
abstract:AIMS:Meticillin-resistant Staphylococcus aureus (MRSA) strains isolated in Tawam Hospital, a tertiary care hospital in the United Arab Emirates, were examined in order to understand the reasons for a doubling of its incidence between 2003 and 2008 while maintaining the same infection control measures. METHODS:All cons...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200436
更新日期:2012-02-01 00:00:00
abstract:AIM:To assess the reliability of local international normalised ratios (INR) using a set of three international reference preparation (IRP) certified freeze dried plasmas. METHODS:55 centres in the United Kingdom and the Republic of Ireland participated; 36 centres employed coagulometers and 19 a manual prothrombin ti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.51.5.356
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:The terminology applied to vascular anomalies has been variable in previously published literature making interpretation suboptimal. The International Society for the Study of Vascular Anomalies (ISSVA) has proposed a revised classification based on clinical features and histopathological findings. This clas...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038240
更新日期:2006-12-01 00:00:00
abstract::A comparison of Skirrow's, Butzler's, Blaser's, Campy-BAP and Preston media for Campylobacter spp was made using human, animal and environmental specimens. Butzler's medium gave the lowest isolation rate and Preston medium, which was the most selective, the highest isolation rate. Enrichment culture using Preston enri...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.1.78
更新日期:1983-01-01 00:00:00
abstract:AIM:Recent events surrounding postmortem practices have necessitated several changes in the way necropsies are conducted both within the medicolegal and hospital settings. These changes have resulted in a striking reduction in the frequency of brain retention for neuropathological examination. Aberdeen Royal Infirmary,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.028274
更新日期:2006-04-01 00:00:00
abstract:AIMS:Clot waveform analysis (CWA) has been reported to extend the interpretation of clotting time measurement. The parameters obtained from successive derivatives of the clotting reaction curves reflect the rates of activation of individual coagulation factors, theoretically dissecting the cascade pathway. This study a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2018-205517
更新日期:2019-03-01 00:00:00
abstract::It was postulated that the short chain fatty acids (SCFA) produced by anaerobic bacteria might serve as microbial markers in purulent material. Eighteen pus specimens from various sources were analysed by gas-liquid chromatography (GLC), and the SCFA detected were compared with the microorganisms isolated by conventio...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1163
更新日期:1979-11-01 00:00:00
abstract::In four persons of one family the existence of an enzymatic defect, presumably consisting of a deficiency of glutaminase in the cells of the renal tubules, is postulated, and is implied by a reduced elimination of ammonia in the urine, by a relatively low urinary pH, and by its increased titratable acidity. The most c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.2.219
更新日期:1965-03-01 00:00:00
abstract::In colonic biopsies of 33 patients with acute colitis caused by campylobacter, salmonella, or shigella immunoglobulin containing cells were determined quantitatively using an indirect immunoperoxidase technique and morphometry with a graphic tablet. The findings were compared with those of 10 patients without histolog...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.7.774
更新日期:1985-07-01 00:00:00
abstract::The effect of radiotherapy on normal buccal mucosa was investigated using the quantitative techniques of cytomorphology (measurement of nuclear and cytoplasmic area) and DNA cytophotometry. These techniques were applied to smears obtained before, during, and after irradiation. Nuclear area and cytoplasmic area increas...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.9.940
更新日期:1989-09-01 00:00:00
abstract::Fourteen formalin fixed and paraffin wax embedded biopsy specimens from 10 women (age range 34-68) presenting with pseudomembranous trigonitis were studied using a combination of anti-oestrogen receptor monoclonal antibodies in an avidin-biotin immunoperoxidase technique. All epithelial areas showing vaginal metaplasi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.9.922
更新日期:1989-09-01 00:00:00
abstract::Using a short series of biochemical tests already in use in many routine laboratories, it is possible to identify strains of the genus Klebsiella and to differentiate K. aerogenes from other types. Marked production of slime is not peculiar to the group and colonial appearance alone is not a satisfactory basis for ide...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.6.617
更新日期:1964-11-01 00:00:00
abstract:AIM:To evaluate the clinical usefulness of three commercially available assays for Her-2/neu oncogene and protein measurements. The Her-2/neu protein is overexpressed, mostly as a result of gene amplification, in 20-30% of human breast cancers, and has been shown to have prognostic and predictive value for treatment wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.5.374
更新日期:2000-05-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract::Two isolates of Proteus mirabilis and four of Esch. coli which would not grow on commonly used commercial sensitivity test media are reported. These organisms appear to be resistant to co-trimoxazole, are exacting towards thymidine, and five of six were isolated from patients who had been treated with co-trimoxazole. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.12.1086
更新日期:1972-12-01 00:00:00
abstract::Gastrointestinal cell proliferation plays an important role in the maintenance of the integrity of the gastrointestinal system. The study of gastrointestinal proliferation kinetics allows a better understanding of the complexity of the system, and also has important implications for the study of gastrointestinal carci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.52.5.321
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND:Fibrinogen plays a crucial role in the pathophysiology of tumour cell growth, invasion and metastasis. The aim of this study was to evaluate the prognostic significance of pretreatment plasma fibrinogen levels in patients with diffuse large B cell lymphoma (DLBCL) METHODS: Data from 372 patients with DLBCL, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2015-203356
更新日期:2016-04-01 00:00:00
abstract::Experiments were performed to evaluate five commercial kit assays used for the detection of antibodies to dsDNA. The kits were compared using a performance index score as recommended by the guidelines of the European Committee for Clinical Laboratory Standards. The highest performance score was obtained using the radi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.11.1223
更新日期:1988-11-01 00:00:00
abstract::As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1202
更新日期:1978-12-01 00:00:00
abstract::A commercial latex slide test (Oxoid DR 620) for the identification of Escherichia coli O157 was compared with a standard tube agglutination method using E coli O157 antiserum (Difco). Thirty isolates previously confirmed as E coli O157 were positive by both methods: 30 isolates of E coli of serogroups other than O157...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.10.1109
更新日期:1989-10-01 00:00:00
abstract::Gastroblastoma is a rare gastric epitheliomesenchymal biphasic tumour composed of spindle and epithelial cells, reported by Miettinen et al in a series of three cases in 2009. All those cases arose in stomachs of young adults. Neither the epithelial nor the mesenchymal component displayed sufficient atypia to diagnose...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.074104
更新日期:2010-03-01 00:00:00
abstract:AIMS:To isolate RNA and DNA simultaneously from formalin fixed paraffin wax embedded tissue to assess the clonality of enteropathy associated T cell lymphomas and to analyse it in detail by a non-radioactive method of T cell receptor complementarity determining region 3 (CDR3) spectratyping. METHODS:DNA and RNA were i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.10.811
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:Optimal management of patients with lung cancer requires accurate cell typing of tumours and staging at the time of diagnosis. Endobronchial ultrasound-guided lymph node aspiration as a method of diagnosing and staging lung cancer is a relatively new technique. AIM:To report the use of liquid-based-thin-lay...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038901
更新日期:2007-04-01 00:00:00
abstract::A post-mortem examination, in the case of a 34-year-old man dying suddenly from head injuries sustained in a road accident, showed early cirrhosis of the liver and associated splenomegaly 17 years after an attack of infective hepatitis. The histology of the thymus showed abnormal changes in the medulla, consisting of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.5.445
更新日期:1963-09-01 00:00:00
abstract::The only method currently available to perform Neisseria gonorrhoeae antimicrobial susceptibility testing (Ng-AST) requires a viable organism obtained by culture. Reports of in vitro resistance to extended-spectrum cephalosporins, the treatment of choice for gonorrhoea, coupled with increasing gonorrhoea diagnoses is ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202392
更新日期:2014-11-01 00:00:00
abstract::A case of pigmented basal cell carcinoma developing in a smallpox revaccination site is presented. Any progressive change within a smallpox vaccination scar should be thoroughly evaluated and treated appropriately after tissue diagnosis. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.2.134
更新日期:1980-02-01 00:00:00
abstract:AIM:To evaluate the use of multimedia enhancements, using a computerised microscope, in the training of microscope skills. METHODS:The HOME microscope provides facilities to highlight features of interest in conjunction with either text display or aural presentation. A pilot study was carried out with 10 individuals, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.4.330
更新日期:1998-04-01 00:00:00
abstract::As patients with multiple myeloma (MM) have a variable clinical course, predictive markers would help determine the appropriate treatment strategy. Clinical staging is commonly used to predict outcome, but tumour marker expression and the underlying genetic changes are increasingly used to assess the biological aggres...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.049585
更新日期:2008-07-01 00:00:00
abstract::The clinical and pathological features of a case of malakoplakia of the adrenal gland occurring in a woman with Escherichia coli infection are described. This lesion mimicked a neoplasm, the true diagnosis only being revealed by histological examination. The light and electron microscopic features are described and it...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.6.606
更新日期:1981-06-01 00:00:00