Method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens.

Abstract:

:A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The morphology of the labelled cells is well preserved for analysis by light microscopy and the preparations obtained can be kept permanently. This method is useful for the analysis of mixed cell proliferations, particularly in leukaemias.

journal_name

J Clin Pathol

authors

Tavares de Castro J,San Miguel JF,Soler J,Catovsky D

doi

10.1136/jcp.37.6.628

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

628-32

issue

6

eissn

0021-9746

issn

1472-4146

journal_volume

37

pub_type

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