Pitfalls of formalin fixation for determination of antineutrophil cytoplasmic antibodies.

Abstract:

:Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). There is controversy over the value of formalin fixation of neutrophils in differentiating antibodies giving selective or preferential reaction with the nuclear or perinuclear area of neutrophils. In a comparative study of 77 sera, formalin fixation caused inconsistency, nonspecific effects, and false positivity owing to enhanced fluorescence. If formalin fixed neutrophils are used in the routine diagnostic laboratory, this will add confusion to the interpretation of the ANCA assay.

journal_name

J Clin Pathol

authors

Chowdhury SM,Broomhead V,Spickett GP,Wilkinson R

doi

10.1136/jcp.52.6.475

keywords:

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

475-7

issue

6

eissn

0021-9746

issn

1472-4146

journal_volume

52

pub_type

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