Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods.

Abstract:

:In situ hybridisation, immunohistochemistry, and morphological analysis for the detection of cytomegalovirus (CMV) were compared in routinely processed tissue sections from a patient with acquired immune deficiency syndrome (AIDS) and widespread CMV infection. Both in situ hybridisation and immunohistochemistry with the monoclonal antibody CCH2 labelled all "owl's eye" cells intensely and, in addition, nuclei of some morphologically normal cells. Quantitative evaluation of the results showed that in situ hybridisation and immunohistochemistry with CCH2 were considerably more sensitive than purely morphological analysis, particularly in tissues with only a few cells infected by CMV. It is further shown that immunohistochemistry with CCH2 detected a higher figure of CMV infected cells than in situ hybridisation. In conclusion, both in situ hybridisation and immunohistochemistry are rapid, sensitive, and specific methods for CMV detection. For routine purposes, however, immunohistochemistry seems to be more suitable.

journal_name

J Clin Pathol

authors

Niedobitek G,Finn T,Herbst H,Gerdes J,Grillner L,Landqvist M,Wirgart BZ,Stein H

doi

10.1136/jcp.41.9.1005

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

1005-9

issue

9

eissn

0021-9746

issn

1472-4146

journal_volume

41

pub_type

杂志文章
  • Morules in endometrial carcinoma and benign endometrial lesions differ from squamous differentiation tissue and are not infected with human papillomavirus.

    abstract:BACKGROUND:Squamous differentiation/squamous metaplasia is often associated with endometrial adenocarcinoma and benign lesions, such as endometrial hyperplasia and chronic endometritis. Morules have distinct histological characteristics, and are referred to as squamous metaplasia or squamoid metaplasia. AIM:To focus o...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2004.017996

    authors: Chinen K,Kamiyama K,Kinjo T,Arasaki A,Ihama Y,Hamada T,Iwamasa T

    更新日期:2004-09-01 00:00:00

  • Screening for lupus anticoagulant and anticardiolipin antibodies in women with fetal loss.

    abstract::Sixty six women with first or second trimester fetal loss were investigated for the presence of lupus anticoagulant by routine coagulation tests and the dilute Russell's viper venom time with a platelet neutralisation procedure, and for raised anticardiolipin antibodies by an enzyme linked immunosorbent assay. Of 35 w...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.44.1.45

    authors: Creagh MD,Malia RG,Cooper SM,Smith AR,Duncan SL,Greaves M

    更新日期:1991-01-01 00:00:00

  • Use of polymerase chain reaction for early identification of Mycobacterium tuberculosis in positive cultures.

    abstract:AIMS:To develop a readily applicable polymerase chain reaction (PCR) based technique which would permit the identification of Mycobacterium tuberculosis complex isolates from Bactec phials at an earlier stage than currently available methods. METHODS:Mycobacterial cells cultured in Bactec 12B medium were harvested by ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.45.7.601

    authors: Cormican MG,Barry T,Gannon F,Flynn J

    更新日期:1992-07-01 00:00:00

  • Primary squamous cell carcinoma of the terminal ileum.

    abstract::A case of squamous cell carcinoma of the terminal ileum with no underlying duplication or inflammatory disorder is described. The neoplasm seemed to have originated from the surface epithelium, invading the wall and metastasising to the regional lymph nodes. The 65 year old patient was free of disease three years afte...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.44.3.253

    authors: Platt CC,Haboubi NY,Schofield PF

    更新日期:1991-03-01 00:00:00

  • A comparison of complement fixation, indirect immunofluorescence for viral late antigens, and anti-complement immunofluorescence tests for the detection of cytomegalovirus specific serum antibodies.

    abstract::It was shown that, on average, the titres of the sera obtained by both the anti-complement immunofluorescence (ACIF) and indirect immunofluorescence for viral late antigens (IFA-LA) procedures were approximately eight times higher than those obtained by complement fixation. There was no significant difference between ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.31.9.827

    authors: Griffiths PD,Buie KJ,Heath RB

    更新日期:1978-09-01 00:00:00

  • Interpretation of respiratory tract histology in cot deaths.

    abstract::The degree of inflammation in the trachea, bronchi, and lungs of 139 cot deaths has been analysed and each case allocated to one of four groups. In group I the changes were considered to be serious enough to have caused death, while group II cases showed similar abnormalities but of a less severe nature, and in this g...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.28.11.899

    authors: Tapp E,Jones DM,Tobin JO

    更新日期:1975-11-01 00:00:00

  • Atypical aspirates of the breast: a dilemma in current cytology practice.

    abstract:AIMS:The probabilistic approach is widely adopted for breast fine needle aspiration cytology. However, a definite cytological diagnosis is not always possible for C3 (atypia) cases, which poses a management dilemma as this represents a mixed category of benign and malignant cases. It would be beneficial to be able to p...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,多中心研究

    doi:10.1136/jclinpath-2016-204138

    authors: Yu SN,Li J,Wong SI,Tsang JYS,Ni YB,Chen J,Tse GM

    更新日期:2017-12-01 00:00:00

  • Friend leukaemia integration-1 expression in malignant and benign tumours: a multiple tumour tissue microarray analysis using polyclonal antibody.

    abstract:BACKGROUND:Friend leukaemia integration-1 (FLI-1) antibody is a useful marker for Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and vascular tumours. However, it is also expressed in subsets of lymphoblastic lymphoma, Merkel cell carcinoma (MCC) and desmoplastic small round cell tumour (DSRCT). AIM:To de...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2006.039230

    authors: Mhawech-Fauceglia P,Herrmann FR,Bshara W,Odunsi K,Terracciano L,Sauter G,Cheney RT,Groth J,Penetrante R,Mhawech-Fauceglia P

    更新日期:2007-06-01 00:00:00

  • Comparison of antibiotic discs from different sources.

    abstract::Antibiotic discs from Oxoid, Mast, AB Biodisk, Difco, and Baltimore Biological Laboratories were compared, where discs of similar antibiotic content were available, in diffusion-sensitivity tests against organisms of known sensitivity. Discs from Oxoid and Mast gave zones 1-5 mm larger than discs from other manufactur...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.28.10.779

    authors: Brown DF,Kothari D

    更新日期:1975-10-01 00:00:00

  • A study in vitro of the sensitivity to antibiotics of Bacteroides fragilis.

    abstract::During a two-year period of observation Bacteroides species were isolated from specimens of pus and vaginal swabs from 115 patients in this hospital. Thirty-five representative strains proved on examination to be Bacteroides fragilis. Minimal inhibitory and minimal bactericidal concentrations of six antibiotics for th...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.21.4.432

    authors: Ingham HR,Selkon JB,Codd AA,Hale JH

    更新日期:1968-07-01 00:00:00

  • Fatal Campylobacter jejuni infection in a patient splenectomised for thalassaemia.

    abstract::A 35 year old man with a fatal Campylobacter jejuni infection is described. He had HbE/beta zero thalassaemia and had undergone splenectomy nine months previously for hypersplenism; he also had chronic hepatitis C infection. He presented with high grade fever but no gastrointestinal symptoms and rapidly progressed to ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.50.5.436

    authors: Jackson N,Zaki M,Rahman AR,Nazim M,Win MN,Osman S

    更新日期:1997-05-01 00:00:00

  • Caveolin-1 expression as a prognostic marker in triple negative breast cancers of Asian women.

    abstract:BACKGROUND:Triple-negative breast cancers (TNBCs) are defined by their lack of oestrogen receptor, progesterone receptor and epidermal growth factor receptor 2. Although heterogeneous, the majority are aggressive and treatment options are limited. Caveolin acts as tumour suppressor or promoter depending on the cancer t...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2017-204495

    authors: Yeong J,Thike AA,Ikeda M,Lim JCT,Lee B,Nakamura S,Iqbal J,Tan PH

    更新日期:2018-02-01 00:00:00

  • Multicentric evaluation of analytical performances digital morphology with respect to the reference methods by manual optical microscopy.

    abstract:AIMS:Optical microscopic (OM) evaluation of peripheral blood (PB) cells is still a crucial step of the laboratory haematological workflow. The morphological cell analysis is time-consuming and expensive and it requires skilled operator. To address these challenges, automated image-processing systems, as digital morphol...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2020-206857

    authors: Da Rin G,Benegiamo A,Di Fabio AM,Dima F,Francione S,Fanelli A,Germagnoli L,Lorubbio M,Marzoni A,Pajola R,Pipitone S,Rolla R,Seghezzi M,Baigorria Vaca MDC,Bartolini A,Buoro S

    更新日期:2020-09-14 00:00:00

  • Nasal polyposis in Peutz-Jeghers syndrome: a distinct histopathological and molecular genetic entity.

    abstract:BACKGROUND:Peutz-Jeghers syndrome (PJS) is an autosomal dominant hamartomatous polyposis syndrome of the gastrointestinal tract, caused by a germline STK11/LKB1 mutation. Nasal polyposis was described in the original report by Peutz. Recently, a molecular-genetic association between nasal polyposis and PJS has been rep...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2005.036418

    authors: de Leng WW,Westerman AM,Weterman MA,Jansen M,van Dekken H,Giardiello FM,de Rooij FW,Paul Wilson JH,Offerhaus GJ,Keller JJ

    更新日期:2007-04-01 00:00:00

  • Stromal CD10 expression in mammary fibroadenomas and phyllodes tumours.

    abstract:BACKGROUND/AIMS:CD10 (CALLA) has recently been reported to be expressed in spindle cell neoplasia, and has been used to differentiate endometrial stromal sarcoma from leiomyoma and leiomyosarcoma. In the breast, myoepithelial cells express CD10, but there are few studies of the expression of CD10 in mammary fibroepithe...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2004.020917

    authors: Tse GM,Tsang AK,Putti TC,Scolyer RA,Lui PC,Law BK,Karim RZ,Lee CS

    更新日期:2005-02-01 00:00:00

  • Use of Sephadex column chromatography in the assessment of thyroid status.

    abstract::A method is described for assessing thyroid status using Sephadex column chromatography of serum samples with added (125)I triiodothyronine. The method is simple and inexpensive. It gave a correct diagnostic result in 97% of 258 euthyroid, 90% of 80 hyperthyroid, 78% of 59 hypothyroid, and 67% of 50 euthyroid, ill pat...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.20.2.170

    authors: Gimlette TM

    更新日期:1967-03-01 00:00:00

  • Coexistence of an endocrine tumour in a serous cystadenoma (microcystic adenoma) of the pancreas, an unusual association.

    abstract::A pancreatic endocrine tumour arising within a serous cystadenoma is reported. A 49 year old woman was admitted with a history of epigastric pain, nausea, vomiting, and weight loss of two months duration. She had been diabetic for 12 years. An epigastric mass was palpated in the physical examination, and computed tomo...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.53.10.800

    authors: Ustün MO,Tuğyan N,Tunakan M

    更新日期:2000-10-01 00:00:00

  • Non-alcoholic steatohepatitis: a common cause of progressive chronic liver injury?

    abstract:AIM:To investigate the natural history of patients with non-alcoholic steatohepatitis by means of a prospective histological study. METHODS:One thousand five hundred and seventy one patients underwent liver biopsy at the Western Infirmary in Glasgow during the 10 year period 1985 to 1994. All biopsies were reported by...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.55.9.689

    authors: Evans CD,Oien KA,MacSween RN,Mills PR

    更新日期:2002-09-01 00:00:00

  • Changes in cytomorphology of childhood lymphoblastic leukaemia at the time of disease relapse. Childhood Leukaemia Working Party of the United Kingdom Medical Research Council.

    abstract:AIMS:Children in a United Kingdom national trial for relapsed non-B lymphoblastic leukaemia (ALL) had their diagnostic and relapse marrow cytomorphology compared to see what changes occur during the evolution of the disease. METHODS:Each relapse slide was assessed blindly for French American British (FAB) type and oth...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.11.1051

    authors: Lilleyman JS,Stevens RF,Hann IM,Gibson BE,Britton JA,Darbyshire PJ,Oakhill A

    更新日期:1995-11-01 00:00:00

  • Cytological changes preceding cervical cancer.

    abstract::Cervical smears were reviewed from 62 women who developed squamous carcinoma of the cervix up to 18 years later. The findings indicate that the prevention of cervical cancer by screening depends very largely on the detection of severe dyskaryosis. In this series there was no evidence that mild dyskaryosis was a foreru...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.47.3.278

    authors: Robertson JH,Woodend B,Elliott H

    更新日期:1994-03-01 00:00:00

  • Examination of International Normalised Ratio (INR) imprecision by comparison of exact and approximate formulas. Intermountain Laboratory Data Project.

    abstract:AIM:To evaluate the accuracy of the approximate linear formula of International Normalised Ratio (INR) imprecision by formal mathematical analysis. METHODS:Using probability theory, an exact formula for the coefficient of variation (CV) of the INR was derived. The CV from the approximate formula was compared with the ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.47.7.635

    authors: Bennett ST,Critchfield GC

    更新日期:1994-07-01 00:00:00

  • Digital slide and virtual microscopy based routine and telepathology evaluation of routine gastrointestinal biopsy specimens.

    abstract:AIMS:To evaluate a recently developed digital slide and virtual microscope system, and to compare this method with optical microscopy on routine gastrointestinal biopsy specimens in both local and remote access modes. METHODS:A fully computer controlled commercial microscope was used. The scanning program included obj...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.56.6.433

    authors: Molnar B,Berczi L,Diczhazy C,Tagscherer A,Varga SV,Szende B,Tulassay Z

    更新日期:2003-06-01 00:00:00

  • Tropical myositis: ultrastructural studies.

    abstract::Specimens of muscle were obtained from non-suppurating lesions of nine patients with tropical myositis. When examined in an electron microscope, these revealed patchy myocytolysis with loss of band structure. Perimysial cells were also degenerate. Sections from two out of nine patients revealed intracellular vesicles,...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.29.12.1081

    authors: Taylor JF,Fluck D,Fluck D

    更新日期:1976-12-01 00:00:00

  • The assay and properties of labile factor (factor V).

    abstract::Human oxalated plasma stored at 4 degrees C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreem...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.13.6.457

    authors: QUICK AJ

    更新日期:1960-11-01 00:00:00

  • ESTIMATION OF ALPHA-AMINO NITROGEN IN PLASMA AND URINE BY THE COLORIMETRIC NINHYDRIN REACTION.

    abstract::Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves th...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.17.2.150

    authors: MATTHEWS DM,MUIR GG,BARON DN

    更新日期:1964-03-01 00:00:00

  • Cultural method for large-scale screening for Chlamydia trachomatis genital infection.

    abstract::Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.34.7.712

    authors: Mallinson H,Sikotra S,Arya OP

    更新日期:1981-07-01 00:00:00

  • Interpretation of serum total calcium: effects of adjustment for albumin concentration on frequency of abnormal values and on detection of change in the individual.

    abstract::Serum total calcium was measured in 1693 patients during a four-month period. We examined the effects of adjustment for albumin concentration on the interpretation of single measurements of serum total calcium and on the variation of series of measurements in individual patients. Markedly abnormal total calcium concen...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.32.1.56

    authors: Payne RB,Carver ME,Morgan DB

    更新日期:1979-01-01 00:00:00

  • Hepatitis C virus replication in hepatocellular carcinoma.

    abstract::Hepatitis C virus (HCV) replication is reported in both tumour and non-tumour tissue in a case of hepatocellular carcinoma. Viral replication was established by showing the presence of minus strand HCV RNA by PCR amplification, after excluding residual reverse transcriptase activity of Taq polymerase. No minus strand ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.9.880

    authors: Niu J,Kumar U,Monjardino J,Goldin R,Rosin D,Thomas HC

    更新日期:1995-09-01 00:00:00

  • Immunocytological analysis of the Tn associated antigen 83D4 in serous effusions from patients with cancer: comparison with Tn soluble glycoprotein.

    abstract:AIMS:To determine whether the monoclonal antibody (MoAb) 83D4, previously shown to be highly specific for carcinoma cells, can be used as an immunocytological marker to discriminate between benign and malignant cells in serous effusions; and to test for a correlation between expression of the antigen reacting with MoAb...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.5.433

    authors: Beuzelin-Yvraut M,Bourguignat A,Phillips E,Roseto A,Osinaga E

    更新日期:1995-05-01 00:00:00

  • The use of an anaerobic incubator for the isolation of anaerobes from clinical samples.

    abstract::An anaerobic incubator was compared with a standard jar system for the isolation of anaerobes from clinical material. Seventy specimens were selected as likely to yield anaerobes: 342 different anaerobes were isolated in the incubator and 347 in anaebrobic jars. These included Bacteroides spp (43%), Peptococcus spp (2...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.10.1158

    authors: Berry PL,Taylor E,Phillips I

    更新日期:1982-10-01 00:00:00