Abstract:
:Human oxalated plasma stored at 4 degrees C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreement is not obtained with plasma stored at 37 degrees C. The stability of labile factor is closely associated with ionized calcium. The addition of thrombin to fresh oxalated plasma causes an apparent hyperactivity of labile factor, but this is completely removed by adsorption with Ca(3)(PO)(2). Oxalated plasma when adsorbed with Ca(3)(PO(4))(2) before treatment with thrombin does not develop this adventitious activity, nor does it occur in stored plasma treated with thrombin. The seemingly high labile factor activity in serum can be explained by the activation of this factor which is independent of labile factor but acts synergistically with it. The true labile factor concentration can be determined only after the accelerator is removed by adsorption with Ca(3)(PO(4))(2). A close agreement between the consumption of prothrombin and the loss of labile factor during clotting is observed.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
QUICK AJdoi
10.1136/jcp.13.6.457keywords:
subject
Has Abstractpub_date
1960-11-01 00:00:00pages
457-62eissn
0021-9746issn
1472-4146journal_volume
13pub_type
杂志文章abstract::A method utilizing a general dehydrogenase reaction has been used to demonstrate early gross myocardial infarctions. The procedure takes advantage of substrate and enzyme loss from the damaged myocardium. In the viable muscle, where endogenous substrates, coenzymes, and dehydrogenases are present, reduction of Nitro-B...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.5.479
更新日期:1966-09-01 00:00:00
abstract:BACKGROUND:Peutz-Jeghers syndrome (PJS) is an autosomal dominant hamartomatous polyposis syndrome of the gastrointestinal tract, caused by a germline STK11/LKB1 mutation. Nasal polyposis was described in the original report by Peutz. Recently, a molecular-genetic association between nasal polyposis and PJS has been rep...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.036418
更新日期:2007-04-01 00:00:00
abstract::The sensitivity of 12 commonly used anti-HIV-1/HIV-2 enzyme immunoassays was evaluated. The assays, each of which utilises at least one synthetic HIV antigen, were tested against a panel of 1092 specimens previously designated anti-HIV positive. In a total of 13 104 tests there were eight false negative results attrib...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.5.490
更新日期:1995-05-01 00:00:00
abstract::A case of B-CLL which was complicated by chronic renal failure due to leukaemic infiltration of the kidney is reported. Treatment with chlorambucil, prednisolone, and renal bed irradiation resulted in a substantial improvement in renal function which persisted until the the patient's death from marrow failure some eig...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.12.1131
更新日期:1993-12-01 00:00:00
abstract::The activities of phosphohexose isomerase and 6-phosphogluconate dehydrogenase have been studied in the vaginal fluid of women from the gynaecological departments. Women from these departments gave a very high false positive rate, though no cases of carcinoma in situ were not detected by estimating phosphohexose isome...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.4.378
更新日期:1966-07-01 00:00:00
abstract::The use of the dye calcon (1-(2 hydroxy-1-naphthylazo)-2-naphthol-4 sulphonic acid) for the estimation of calcium using E.D.T.A. and a commercial photoelectric titrimeter is described. The interfering effects of magnesium and phosphate have been overcome. The method has been extended to estimations on biological mater...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.1.18
更新日期:1963-01-01 00:00:00
abstract::Advanced non-small cell lung cancer samples are tested for epidermal growth factor receptor (EGFR) gene mutations. Their detection by direct sequencing is time-consuming. Conversely, the length analysis of fluorescently labelled PCR products is easier. To avoid labelled primers and the automated capillary electrophore...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201730
更新日期:2013-11-01 00:00:00
abstract::Streptex was compared to routine laboratory identification methods available. The results from Streptex sometimes required several attempts before final identification could be achieved. In the main, group D streptococci other than Strep. faecalis failed to group with the Streptex antisera, and this method cannot ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.12.1234
更新日期:1979-12-01 00:00:00
abstract:AIM:To compare the attitudes of students towards the necropsy at different stages of their undergraduate career. METHOD:Students in the first, fourth and sixth academic years (n = 283) were asked to respond anonymously to a questionnaire comprised of 26 attitude statements. These statements dealt with the importance o...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.1.64
更新日期:1997-01-01 00:00:00
abstract::Acute myocardial disease, which may be associated with abnormal platelet activity, has been reported after routine immunisation. Thirty-two army apprentices undergoing immunisation were studied for changes in the heparin neutralising activity (HNA) of platelet poor plasma. HNA increased after immunisation. This increa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1152
更新日期:1979-11-01 00:00:00
abstract::Severe osteomalacia (confirmed by the examination of thin undecalcified bone biopsy sections) associated with hypophosphataemia developed in a 60 year old woman. A skeletal x-ray survey showed a lytic lesion in the right proximal femur, and this was curetted, showing a vascular tumour. The patient's symptoms improved ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.4.446
更新日期:1987-04-01 00:00:00
abstract:AIMS:To investigate how the biopsy Gleason score (GS) and the clinical risk classification have been changed by the reporting rules. METHODS:565 prostate biopsy specimens were reassessed. Each Gleason pattern, 1 to 5, was interpreted according to the modified Gleason grading system proposed by the International Societ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2008.060632
更新日期:2009-03-01 00:00:00
abstract::The excretion of 2,3-dinor-6-keto prostaglandin F1 alpha, a major urinary metabolite of prostacyclin, and the formation of thromboxane B2, a stable metabolite of thromboxane A2, by platelets stimulated by adenosine diphosphate, were studied in alcoholics, who had been admitted for detoxification. Once prolonged heavy ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.5.512
更新日期:1987-05-01 00:00:00
abstract::Incomplete expression of human haemoglobin beta-chains at birth may lead to difficulty in the early demonstration of an inherited beta-chain variant. In this case, the rare unstable variant, Hb Tacoma beta30 (B12) arg leads to ser, although not present in cord blood in sufficient amounts to be easily detected by routi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.9.883
更新日期:1978-09-01 00:00:00
abstract:AIM:To develop a unified diagnostic approach for the detection of human papillomavirus (HPV) DNA in skin and mucosal biopsies from both immunocompetent and immunosuppressed individuals using a degenerate polymerase chain reaction (PCR) method. METHODS:The sensitivity and specificity of three published degenerate prime...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.8.606
更新日期:1998-08-01 00:00:00
abstract::A questionnaire was circulated to clinical biochemistry laboratories in the North West Thames region of the United Kingdom requesting information on extralaboratory equipment. Data on the types and numbers of instruments in use, their relationship with the laboratory, and quality assurance procedures were obtained. La...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.9.925
更新日期:1988-09-01 00:00:00
abstract::The production of heparinase by a wide range of anaerobic bacteria isolated from clinical specimens was investigated. None of the 29 strains of Bacteroides fragilis produced heparinase. Of 62 other Bacteroides tested, only two of four strains of B ovatus, two of three strains of B thetaiotaomicron, and two of four str...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.4.384
更新日期:1987-04-01 00:00:00
abstract::The occurrence of a severe generalized haemorrhagic state in an obstetric case due to a heparin-like anticoagulant is described. This appeared in the post-partum period apparently following a compatible blood transfusion. The pattern of results found in conventional laboratory tests for elucidating acute blood coagula...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.2.108
更新日期:1963-03-01 00:00:00
abstract::Leucocyte ascorbate (LA) and serum ascorbate (SA) were measured in patients who had sustained an acute cerebrovascular accident. There was a significant fall in LA and Sa within 24 hours of the incident and this persisted for several weeks. The "stress" of the event resulted in a rise in serum cortisol concentration a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.2.195
更新日期:1982-02-01 00:00:00
abstract::Inherited biochemical defects may present with acute life-threatening illness with a high mortality and morbidity. Some are treatable and have a good outcome with early appropriate intervention. However, because of their rarity, diagnosis is often delayed; they are not considered or investigated appropriately. This is...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2009.067884
更新日期:2011-03-01 00:00:00
abstract:AIMS:To determine the sensitivity of commercially available diagnostic assays for Chlamydia trachomatis using a simple method. METHODS:Nine commercial assays and an "in-house" polymerase chain reaction (PCR) were evaluated using serial dilutions of a laboratory grown H serovar--four of them using a laboratory grown E ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.5.408
更新日期:1993-05-01 00:00:00
abstract:AIMS:To show that medullary carcinomas of the thyroid are morphologically indistinguishable from gut carcinoids: the value of histochemistry in their identification and differential diagnosis from metastatic carcinoid tumours to the thyroid and some follicular cell neoplasms. METHODS:15 thyroid medullary carcinomas wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.2.113
更新日期:1993-02-01 00:00:00
abstract::Primary low grade marginal zone B cell lymphoma (MZL) of the breast and localised mammary amyloidosis are exceedingly rare entities. This report describes the case of a woman with long standing Sjögren's syndrome presenting with asymptomatic MZL of the breast showing plasmacytic differentiation, associated with local ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.1.74
更新日期:2003-01-01 00:00:00
abstract::Rabbit antiserum, elicited by the intravenous injection of a strain of Campylobacter jejuni heated to 100 degrees C, cross reacted strongly with all other thermophilic campylobacters tested as well as with "C pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. Antisera to formali...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.9.1007
更新日期:1984-09-01 00:00:00
abstract::Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). Ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.6.475
更新日期:1999-06-01 00:00:00
abstract::Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.4.378
更新日期:1978-04-01 00:00:00
abstract::IgD myeloma is a rare disorder with a very poor prognosis. An unusual case is reported where prolonged remission was obtained. Immunohistochemical staining of the bone marrow trephine biopsy specimen proved invaluable in documenting early relapse. It is suggested that immunohistochemistry should be adopted more widely...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.8.773
更新日期:1994-08-01 00:00:00
abstract::The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.3.243
更新日期:1980-03-01 00:00:00
abstract::Patients with congenital lung cysts are at increased risk of developing carcinoma, but the mechanisms concerned are not clear. The case of a young adult who developed a bronchioloalveolar carcinoma associated with a cystic congenital adenomatoid malformation is reported. The adjacent lung showed an unusual intra-alveo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.6.612
更新日期:1987-06-01 00:00:00
abstract:AIMS:Personalised breast cancer therapy requires pathological characterisation of tumours. The proliferative index, based on Ki67, is pivotal, but a standard method has not been established. Here we look for an easy and practical way to evaluate Ki67. METHODS:Immunohistochemical staining of estrogen receptors, progest...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202695
更新日期:2015-05-01 00:00:00