Evaluation and comparison of neutrophil bipolar shape formation with a migration assay.

Abstract:

:The neutrophil shape change response to a chemotactic formylpeptide was assessed. Neutrophil bipolar shape formation (BSF) was also simultaneously assessed with a Boyden chamber-based neutrophil migration assay. Both assays were precise and relatively reproducible; the average coefficient of variation for the BSF assay was 9.6% and 9.2% for the migration assay. In a blind study the BSF assay showed 100% sensitivity at detecting subjects with known abnormal neutrophil migration. Unlike the migration assay, the BSF assay does not require isolated neutrophils, reducing possible cell activation and monitoring the cell response under more physiological conditions. Small blood samples--1 ml or less compared with 20-40 ml for the migration assay--are used, and the method is technically simple. Results are available within 40 minutes, and routine (EDTA) blood samples are used. It is concluded that the BSF assay is a suitable motility screening test for both the clinical and pharmacological examination of the movement of polymorphonuclear leucocytes.

journal_name

J Clin Pathol

authors

Lord RA,Roath S

doi

10.1136/jcp.43.4.342

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

342-5

issue

4

eissn

0021-9746

issn

1472-4146

journal_volume

43

pub_type

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