Abstract:
BACKGROUND/AIMS:The programmed cell death receptor 1 (PD-1) checkpoint inhibitor, nivolumab, has been approved for the treatment of metastatic renal cell carcinoma (RCC). However, the understanding of the expression and distribution of PD ligand 1 (PD-L1) in the tumour immune microenvironment and its prognostic role in an Asian cohort is limited. Our group investigated PD-L1 protein expression in a cohort of Asian patients with RCC of mixed ethnicity, using two commercially available antibody clones. METHODS:E1L3N and SP263 anti-PD-L1 clones were used to categorise RCCs of various histological subtypes, diagnosed at our institution between 1995 and 2008, into PD-L1-positive or PD-L1-negative groups, based on a 1% Tumour Proportion Score (TPS) cut-off. RESULTS:In total, 267 (83%) clear cell (cc)RCC and 55 (17%) non-ccRCC cases were studied. Overall PD-L1 protein expression rates for the entire cohort were 13% and 8% for the E1L3N and SP263 clones, respectively. Patients bearing PD-L1-positive tumours experienced significantly decreased disease-free survival (DFS; E1L3N: p=0.01; SP263: p=0.03) but not overall survival, compared with those with PD-L1-negative tumours. Multivariate survival analysis further confirmed the results of the E1L3N clone (HR 1.85, 95% CI 1.10 to 3.13, p=0.02), but not SP263, after adjusting for pathological stage, histological subtype and grade. The addition of PD-L1 (E1L3N) TPS to clinicopathological features significantly increased the prognostic value for DFS (∆LRχ2=5.25; p=0.022), compared with clinicopathological features alone. CONCLUSIONS:PD-L1 protein expression was associated with an unfavourable prognosis in our study cohort. PD-L1 (E1L3N) expression was an independent prognostic indicator of clinical outcome in all RCCs when using a 1% cut-off.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Yeong J,Zhao Z,Lim JCT,Li H,Thike AA,Koh VCY,Teh BT,Kanesvaran R,Toh CK,Tan PH,Khor LYdoi
10.1136/jclinpath-2019-206092subject
Has Abstractpub_date
2020-08-01 00:00:00pages
463-469issue
8eissn
0021-9746issn
1472-4146pii
jclinpath-2019-206092journal_volume
73pub_type
杂志文章abstract:AIMS:The aim of this study was to assess the prevalence and drug resistance of Ureaplasma species and Mycoplasma hominis in outpatients with genital manifestation from 2005 to 2013 in Hangzhou, China. METHODS:A total of 2689 female and 2336 male patients with various genital symptoms were included in this study. Speci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202248
更新日期:2014-09-01 00:00:00
abstract:AIMS:To develop a specific radioimmunoassay for the routine determination of serum vitamin B12. METHODS:Antisera were raised in rabbits by immunisation with the monocarboxylic acid derivative of cyanocobalamin coupled to human serum albumin. Antibody titres and affinities were determined and the antiserum giving the h...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.4.328
更新日期:1992-04-01 00:00:00
abstract::Rosetting and non-rosetting lymphocytes collected from normal individuals were stained for the presence of beta-glucuronidase, periodic-acid Schiff activity, gamma glutamyl transpeptidase, acid phosphatase, and alpha-naphthyl butyrate esterase. Lymphocytes which formed rosettes with sheep erythrocytes and non-rosette ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.7.653
更新日期:1977-07-01 00:00:00
abstract::As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1202
更新日期:1978-12-01 00:00:00
abstract::A monoclonal antibody, produced by peptide immunisation was used to detect the distribution of p62c/myc by immunohistology in normal and coeliac small intestinal mucosa. The effect of gluten in four treated coeliac patients was investigated by taking serial jejunal biopsy specimens for six hours after a 10 g oral glut...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.3.307
更新日期:1987-03-01 00:00:00
abstract::A histochemical method is described of differentiating between normal and neoplastic cells in plasma using the activity of various enzymes characteristic of plasma cells. It was found that in both types of cell increased or diminished activity of cytoenzymes is a useful means of distinguishing between normal and abnor...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.3.345
更新日期:1965-05-01 00:00:00
abstract:AIMS:Soft tissue tumours are a heterogeneous group of neoplasms that can arise at almost every anatomical site. As they often show similar clinical and radiological findings, histology is the definitive diagnostic method and it is crucial that the surgical pathology report contains accurate, useful information for mana...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203495
更新日期:2016-09-01 00:00:00
abstract::Although Neisseria species other than N gonorrhoeae and N meningitidis normally comprise part of the commensal bacterial flora of the oropharynx, they may occasionally act as opportunistic pathogens. Infections in which these organisms have been implicated include cases of endocarditis, meningitis, septicaemia, otitis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.36.2.213
更新日期:1983-02-01 00:00:00
abstract:AIMS:Congenital neutropenia (CN) is a rare inherited disease that results in recurrent, life-threatening bacterial infections due to a deficiency of mature neutrophils. They are usually caused by heterozygous ELANE mutations although mutations in other genes like HAX-1, G6PC3 and GFI1 have also been reported. Identifyi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206306
更新日期:2020-06-01 00:00:00
abstract::A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.9.766
更新日期:1990-09-01 00:00:00
abstract::A 72 year old woman presented with a suspected myocardial infarction. An echocardiograph showed no acute changes but her plasma creatine kinase (CK) activity was increased at 343 U/l (< 175 normal range). The apparent creatine kinase-MB activity by a CK-M subunit immunoinhibition assay was 350 U/l. In view of the disc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.6.552
更新日期:1994-06-01 00:00:00
abstract::Sodium-alpha-naphthylamine-4-sulphonate (Naphthionin) appears to shorten the bleeding time in normal subjects and to be of value in the management of the bleeding defect in patients with thrombocytopenia when given by intravenous or intramuscular injection. A good response was achieved in three patients suffering from...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.6.680
更新日期:1964-11-01 00:00:00
abstract::The Compu-pet 100 diluter/dispenser is a versatile and accurate instrument which can be employed for a wide variety of microbiological tests and techniques. Time in performing tests is often more than halved when the diluter is used. Large numbers of tests can be performed with minimal fatigue. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.1.37
更新日期:1975-01-01 00:00:00
abstract::The Kallestad Corporation recently suggested that their new buffer system for the nephelometric detection of rheumatoid factor conferred advantages over existing systems. Two rate nephelometric procedures, the Kallestad QM-300 and the Beckman ICS-II (RF), were therefore compared. Sera (n = 157) were selected on the ba...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.3.243
更新日期:1990-03-01 00:00:00
abstract::The amyloid deposits in 21 renal biopsy specimens were subjected to a detailed immunohistochemical analysis using a panel of antibodies against recognised constituents of tissue amyloid. This was a retrospective study of material originally submitted during the investigation of various renal abnormalities and studied ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.3.200
更新日期:1991-03-01 00:00:00
abstract::Common acute lymphoblastic leukaemia antigen (CALLA) was first characterised in lymphoid leukaemic cells. The antigen is present in different stages of lymphoid cell differentiation as well as in subsets of myeloid cells, and further studies have also shown its presence in non-lymphoid tissues. The recent cloning and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.8.654
更新日期:1990-08-01 00:00:00
abstract:AIMS:To assess changes in volume and complexity of cellular pathology workload after clinical service reorganisation and alterations in pathology reporting practices, and to identify objective measures of change applicable to all cellular pathology departments. The ear, nose, and throat (ENT), head and neck (HN) specia...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.57.1.22
更新日期:2004-01-01 00:00:00
abstract::Aneuploidy is frequently noted in malignant tumours. There is much controversy about its cause and effect in relation to malignant tumours. Failure of the spindle checkpoint caused by mutation of the responsible genes may be one of the important factors for the development of aneuploidy. Telomere dysfunction may also ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.018952
更新日期:2004-12-01 00:00:00
abstract::Programmed death ligand 1 (PD-L1) is the principal ligand of programmed death 1 (PD-1), a coinhibitory receptor that can be constitutively expressed or induced in myeloid, lymphoid, normal epithelial cells and in cancer. Under physiological conditions, the PD-1/PD-L1 interaction is essential in the development of immu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2017-204853
更新日期:2018-03-01 00:00:00
abstract::The macrophage markers non-specific esterase, alpha 1-antitrypsin, alpha 1-antichymotrypsin, and lysozyme were compared with conventional microglial and macrophage stains in the human central nervous system. In a series of specimens from cases of head trauma, conventionally fixed and embedded, the modified Weil-Davenp...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.2.150
更新日期:1984-02-01 00:00:00
abstract::Isolates of Campylobacter jejuni, C coli, C fetus and C laridis were tested for agglutination reactions with a panel of five lectins: Arachis hypogaea, Bauhinia purpurea, Solanum tuberosum, Triticum vulgaris and Wisteria floribunda. Twenty three patterns of agglutination (lectin types) were recorded among 376 isolates...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.11.957
更新日期:1990-11-01 00:00:00
abstract::Gonococcal isolates resistant to ciprofloxacin collected from 2004 to 2005, in the Pretoria region, were characterised using Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST). The isolates were obtained from men presenting with urethritis to primary healthcare clinics and general practitioners. All isolate...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.052191
更新日期:2008-05-01 00:00:00
abstract::Sperm-agglutinating antibodies were present in three (2-6%) of 117 fertile men and in 19 (33-9%) of 56 men who had been vasectomized. Twenty-four of the 56 vasectomized men had been studied before vasectomy; sperm-agglutinating antibodies were present in one (4-3%) compared with eight (33-3%) after vasectomy. No sperm...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.12.1127
更新日期:1976-12-01 00:00:00
abstract::Patients with bacterial infection may show altered membrane permeability of the primary azurophilic lysosomes of blood neutrophils. A new enzyme cytochemical technique, sensitive to increased membrane permeability caused by contact of neutrophils with acetone, saponin, low pH, Streptolysin O, bacteria, and nylon wool,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.7.648
更新日期:1978-07-01 00:00:00
abstract:BACKGROUND:The Sysmex XN haematology instrument performs automatic reflex testing, depending on sample results. A nucleated red blood cell (NRBC) count is provided on all samples. The instrument has a smaller footprint (34%) than previous Sysmex XE analysers. METHODS:An evaluation comparing all results to the Sysmex X...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-200930
更新日期:2012-11-01 00:00:00
abstract::A variety of blood culture media were compared to determine the optimal method for the isolation of Mycobacterium avium-intracellulare (MAI) from the blood of patients with AIDS. Simulated laboratory blood cultures and clinical blood cultures were tested. Glucose broth, Bactec aerobic medium, Kirschner's medium, and B...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.6.687
更新日期:1988-06-01 00:00:00
abstract:AIMS:The Royal College of Pathologists recommend that a median of at least 12 lymph nodes should be harvested during pathological staging of colorectal cancer. It is not always easy to harvest the required number, especially in patients with rectal cancer receiving neoadjuvant therapy. Lymph node revealing solutions, f...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203281
更新日期:2016-06-01 00:00:00
abstract::Chromosome studies were attempted on 97% of necropsies carried out in the Department of Histopathology of the Adelaide Children's Hospital over the four-year period ending May 1981. Results were obtained from 89% of necropsies of which 7.5% had major chromosome abnormalities. The chromosome results are analysed accord...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.2.140
更新日期:1983-02-01 00:00:00
abstract:AIMS:Strong thyroglobulin immunoreactivity within sinus histiocytes in a lymph node draining a papillary thyroid carcinoma was observed in a recent case. This prompted the investigation of whether thyroglobulin immunoreactivity is common in regional lymph nodes in cases of thyroid malignancy. METHODS:Eighty seven lymp...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.4.314
更新日期:2001-04-01 00:00:00
abstract::A study has been made of 523 strains of staphylococci on the basis of biological properties, phage typing, and serology. The value of serology in the identification of pathogenic staphylococci has been assessed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.4.370
更新日期:1970-05-01 00:00:00