Abstract:
:The amyloid deposits in 21 renal biopsy specimens were subjected to a detailed immunohistochemical analysis using a panel of antibodies against recognised constituents of tissue amyloid. This was a retrospective study of material originally submitted during the investigation of various renal abnormalities and studied by a routine protocol including histochemistry, electron microscopy, and immunofluorescence. The presence of an amyloid was confirmed in all 21 cases. Seventeen cases contained P component and either amyloid A (AA) (11 cases) or an immunoglobulin light chain associated amyloid (six cases). Four cases contained amyloid material with unusual immunohistochemical findings; one case had AA and P-component (PC) in the interstitium, one case had lambda light chain and beta-2 microglobulin, one case had kappa light chain and Clq, and one case had lambda light chains only. It was possible, therefore, to identify precisely the amyloid constituents and thereby "type" the amyloid by immunohistochemical means. The availability of the antibodies used and their application using these techniques could simplify the confirmation of clinically suspected amyloidosis.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Fitzmaurice RJ,Bartley C,McClure J,Ackrill Pdoi
10.1136/jcp.44.3.200subject
Has Abstractpub_date
1991-03-01 00:00:00pages
200-4issue
3eissn
0021-9746issn
1472-4146journal_volume
44pub_type
杂志文章abstract::A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.11.1015
更新日期:1977-11-01 00:00:00
abstract::A simple, cheap and readily available test system for biological safety cabinets is described. It depends on the containment of an aerosol of Bacillus subtilis spores generated in a BIRD micronebulizer and the measurement of air flows with an anemometer. The system was set up to survey new equipment but equally valuab...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.7.585
更新日期:1974-07-01 00:00:00
abstract:BACKGROUND/AIMS:To evaluate the need for routine histopathological analysis of enucleated/eviscerated eyes and changes in indications for eye removal. METHODS:Retrospective review of all enucleation/evisceration histopathology reports over 20 years. Clinical history was correlated with pathological findings. Two 10 ye...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.029744
更新日期:2006-02-01 00:00:00
abstract::Serum total calcium was measured in 1693 patients during a four-month period. We examined the effects of adjustment for albumin concentration on the interpretation of single measurements of serum total calcium and on the variation of series of measurements in individual patients. Markedly abnormal total calcium concen...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.1.56
更新日期:1979-01-01 00:00:00
abstract::Twenty five specimens of branchial cyst from the same number of patients have been examined. On staining with haematoxylin and eosin a consistent finding was that the mural lymphoid follicles were always aligned with their mantle zones towards the luminal epithelium. With conventional staining lymphatic sinuses were n...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.7.784
更新日期:1985-07-01 00:00:00
abstract::Neisseria gonorrhoeae is one of the most important causes of sexually transmitted disease. We do not fully understand the pathogenesis of infection with this organism, although recent improvements in immunological and molecular techniques have brought us closer to an answer. These techniques are now also being used to...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.40.9.1088
更新日期:1987-09-01 00:00:00
abstract::Several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. While the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. This study has attempted to determine the prevalence of viral...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.4.377
更新日期:1979-04-01 00:00:00
abstract::Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C trachomatis IgA and IgG antibodies. Serological ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.6.686
更新日期:1984-06-01 00:00:00
abstract::Rates of urinary excretion concomitant with the molecular size distribution of filtered polymer fragments were determined in five normovolaemic men dosed with 30 g/m2 BSA of a species of HES (HES 350/0.60) possessing a M-W of 350 000 combined with an MS of 0.60. Approximately 13% of the total injected dose of HES 350/...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.4.361
更新日期:1981-04-01 00:00:00
abstract:AIM:We aimed to assess the one step nucleic acid amplification (OSNA) assay as an intraoperative method in comparison with frozen sections (FS) for detection of metastasis in sentinel lymph nodes (SLNs) of breast cancer. METHOD:100 SLNs from patients with breast carcinoma were enrolled within a 3-month period. Alterna...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202361
更新日期:2014-12-01 00:00:00
abstract:AIMS:As part of the UK antimicrobial resistance strategy and action plan, the Public Health Laboratory Service (PHLS) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. Most laboratories report urine Gram negative isolates, as "coliforms" according to morpholo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.7.524
更新日期:2002-07-01 00:00:00
abstract::The titre of antibodies to Toxoplasma gondii was compared in 1985 sera by use of an indirect haemagglutination test (ToxHA test--Wellcome Reagents Ltd) and the dye test. Sera from 42 patients with clinical or serological indications of recent toxoplasmosis were also examined to determine if the ToxHA test would be of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.7.644
更新日期:1980-07-01 00:00:00
abstract::Visceral botryomycosis is rare, and documented sites are lung, brain, kidney, liver and prostate. This report describes a rare autopsy case of disseminated visceral botryomycosis, with bulky, grape-like botryomycotic vegetations in the heart, and similar abscesses in the lungs and bone marrow. This is the first such r...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2008.057588
更新日期:2008-08-01 00:00:00
abstract:AIMS:To evaluate the IRIS flow microscope for the examination of urine specimens and to compare its performance with that of manual methods; in addition, to assess its usefulness as a screening system for the detection of significant bacteriuria in a routine clinical laboratory. METHODS:The IRIS system was compared wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.11.844
更新日期:2003-11-01 00:00:00
abstract::A chemical test for the detection of bacteriuria-Uriglox-has been compared with the results obtained on culturing urine obtained by suprapubic aspiration in 351 pregnant or puerperal women. The results of this comparison show a correlation of 96.9%, both false positive and false negative results occurring. ...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.26.5.375
更新日期:1973-05-01 00:00:00
abstract::To determine the functional importance of the low B12 and red cell folate concentrations repeatedly observed in the elderly 200 consecutive patients admitted to a geriatric unit were studied. Forty six of the patients had low serum concentrations of B12 (15), red cell folate (26), or both (five). Serum B12 and red cel...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1179
更新日期:1985-10-01 00:00:00
abstract:AIMS:This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia. METHODS:Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202668
更新日期:2015-03-01 00:00:00
abstract::Serum glycocholic acid (SGC) was measured by radioimmunoassay in 277 samples from 122 children with hepatobiliary disorders and from 23 healthy age-matched controls. In patients with hepatobiliary disease the SGC was more frequently abnormal (83%) than values for serum albumin (7%), prothrombin time (17%), bilirubin (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.9.1011
更新日期:1982-09-01 00:00:00
abstract:OBJECTIVE:To study the expression of nuclear beta-catenin and Ki-67 in patients with normal gestation products (NGP), complete hydatidiform moles (CHM), and choriocarcinoma to elucidate their roles in carcinogenesis and their interrelations. METHODS:Expression of nuclear beta-catenin and Ki-67 was studied by immunohis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026666
更新日期:2006-04-01 00:00:00
abstract:AIMS:Experience in the use of whole slide imaging (WSI) for primary diagnosis in pathology is very limited. We aimed to determine the accuracy of interpretation of WSI compared with conventional light microscopy (CLM) in the diagnosis of routine gynaecological biopsies. METHODS:All gynaecological specimens (n=452) rec...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202524
更新日期:2015-01-01 00:00:00
abstract::Serial dilutions of Clostridium difficile culture filtrates were incubated overnight with HeLa cell monolayers. Cells were fixed in formalin, stained with crystal violet, rinsed, and drained. Cell rounding could be observed microscopically in the stained monolayers. Absorbance of the retained dye on monolayers in the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.6.672
更新日期:1986-06-01 00:00:00
abstract:BACKGROUND:Clinical outcome in patients with diffuse large B cell lymphomas (DLBCL) is highly variable and poorly predictable. Microarray studies showed that patients with DLBCL with a germinal centre B cell-like (GCB) phenotype have a better prognosis than those with an activated B cell-like (ABC) phenotype. The BMI1 ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038752
更新日期:2007-02-01 00:00:00
abstract::An ultramicro electrophoretic technique for the separation of serum lipoproteins is described using agarose gel on microscope slides.The interaction between the agarose gel and lipoproteins has been reduced and the separation between beta- and pre-beta-lipoproteins has been improved over existing methods. A semiquanti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.1.83
更新日期:1971-02-01 00:00:00
abstract:AIM:We examined the genetic alterations in a mother and son with multiple eosinophilic chromophobe renal cell carcinomas (chRCCs) showing no other features. METHODS:Germline DNA and bilateral renal cell carcinoma DNA were genetically analysed by whole-exome sequencing. Candidate gene alterations in the first patient's...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2018-205211
更新日期:2018-10-01 00:00:00
abstract::The test is an application of the method originally described by Boyden (1951) and adapted by Stavitsky and Arquilla (1958). Sera which contain anti-Panton-Valentine leucocidin agglutinate the prepared red cells, often to a very high dilution, and this has provided a useful serological test for the diagnosis of staphy...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.14.2.161
更新日期:1961-03-01 00:00:00
abstract::A new rapid automated method for the identification and classification of microorganisms is described. It is based on the incorporation of 35S-methionine into cellular proteins and subsequent separation of the radiolabelled proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.40.9.1070
更新日期:1987-09-01 00:00:00
abstract:BACKGROUND:Peutz-Jeghers syndrome (PJS) is an autosomal dominant hamartomatous polyposis syndrome of the gastrointestinal tract, caused by a germline STK11/LKB1 mutation. Nasal polyposis was described in the original report by Peutz. Recently, a molecular-genetic association between nasal polyposis and PJS has been rep...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.036418
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:The terminology applied to vascular anomalies has been variable in previously published literature making interpretation suboptimal. The International Society for the Study of Vascular Anomalies (ISSVA) has proposed a revised classification based on clinical features and histopathological findings. This clas...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038240
更新日期:2006-12-01 00:00:00
abstract:PURPOSE:To investigate p53 and hepatoma-derived growth factor (HDGF) expression and their association with clinicopathological features of Ewing family tumour (EFT). EXPERIMENTAL DESIGN:A total of 108 cases of EFT were retrospectively analysed. p53 and HDGF expression were detected using immunohistochemistry, and the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201705
更新日期:2014-03-01 00:00:00
abstract::A blood group change from A2 to Ax is described in a boy who had Fallot's tetralogy, oesophageal atresia, and a tracheo-oesophageal fistula. The change of blood group created a transfusion problem. The unusual serology is discussed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.12.962
更新日期:1975-12-01 00:00:00