Immunohistological characterisation of amyloid deposits in renal biopsy specimens.

Abstract:

:The amyloid deposits in 21 renal biopsy specimens were subjected to a detailed immunohistochemical analysis using a panel of antibodies against recognised constituents of tissue amyloid. This was a retrospective study of material originally submitted during the investigation of various renal abnormalities and studied by a routine protocol including histochemistry, electron microscopy, and immunofluorescence. The presence of an amyloid was confirmed in all 21 cases. Seventeen cases contained P component and either amyloid A (AA) (11 cases) or an immunoglobulin light chain associated amyloid (six cases). Four cases contained amyloid material with unusual immunohistochemical findings; one case had AA and P-component (PC) in the interstitium, one case had lambda light chain and beta-2 microglobulin, one case had kappa light chain and Clq, and one case had lambda light chains only. It was possible, therefore, to identify precisely the amyloid constituents and thereby "type" the amyloid by immunohistochemical means. The availability of the antibodies used and their application using these techniques could simplify the confirmation of clinically suspected amyloidosis.

journal_name

J Clin Pathol

authors

Fitzmaurice RJ,Bartley C,McClure J,Ackrill P

doi

10.1136/jcp.44.3.200

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

200-4

issue

3

eissn

0021-9746

issn

1472-4146

journal_volume

44

pub_type

杂志文章
  • Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy.

    abstract::A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.30.11.1015

    authors: Coleman DV,Russell WJ,Hodgson J,Pe T,Mowbray JF

    更新日期:1977-11-01 00:00:00

  • A test system for the biological safety cabinet.

    abstract::A simple, cheap and readily available test system for biological safety cabinets is described. It depends on the containment of an aerosol of Bacillus subtilis spores generated in a BIRD micronebulizer and the measurement of air flows with an anemometer. The system was set up to survey new equipment but equally valuab...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.27.7.585

    authors: Newsom SW

    更新日期:1974-07-01 00:00:00

  • Twenty year review of histopathological findings in enucleated/eviscerated eyes.

    abstract:BACKGROUND/AIMS:To evaluate the need for routine histopathological analysis of enucleated/eviscerated eyes and changes in indications for eye removal. METHODS:Retrospective review of all enucleation/evisceration histopathology reports over 20 years. Clinical history was correlated with pathological findings. Two 10 ye...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2005.029744

    authors: Saeed MU,Chang BY,Khandwala M,Shivane AG,Chakrabarty A

    更新日期:2006-02-01 00:00:00

  • Interpretation of serum total calcium: effects of adjustment for albumin concentration on frequency of abnormal values and on detection of change in the individual.

    abstract::Serum total calcium was measured in 1693 patients during a four-month period. We examined the effects of adjustment for albumin concentration on the interpretation of single measurements of serum total calcium and on the variation of series of measurements in individual patients. Markedly abnormal total calcium concen...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.32.1.56

    authors: Payne RB,Carver ME,Morgan DB

    更新日期:1979-01-01 00:00:00

  • An immunohistochemical study of branchial cysts.

    abstract::Twenty five specimens of branchial cyst from the same number of patients have been examined. On staining with haematoxylin and eosin a consistent finding was that the mural lymphoid follicles were always aligned with their mantle zones towards the luminal epithelium. With conventional staining lymphatic sinuses were n...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.38.7.784

    authors: Crocker J,Jenkins R

    更新日期:1985-07-01 00:00:00

  • Neisseria gonorrhoeae: a versatile pathogen.

    abstract::Neisseria gonorrhoeae is one of the most important causes of sexually transmitted disease. We do not fully understand the pathogenesis of infection with this organism, although recent improvements in immunological and molecular techniques have brought us closer to an answer. These techniques are now also being used to...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1136/jcp.40.9.1088

    authors: Easmon CS,Ison CA

    更新日期:1987-09-01 00:00:00

  • Viral exposure and abnormal liver function in haemophilia.

    abstract::Several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. While the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. This study has attempted to determine the prevalence of viral...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.32.4.377

    authors: McVerry BA,Ross MG,Knowles WA,Voke J

    更新日期:1979-04-01 00:00:00

  • Serum specific IgA antibody to Chlamydia trachomatis in patients with chlamydial infections detected by ELISA and an immunofluorescence test.

    abstract::Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C trachomatis IgA and IgG antibodies. Serological ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.37.6.686

    authors: Cevenini R,Sarov I,Rumpianesi F,Donati M,Melega C,Varotti C,La Placa M

    更新日期:1984-06-01 00:00:00

  • Urinary excretion kinetics of hydroxyethyl starch 350/0.60 in normovolaemic man.

    abstract::Rates of urinary excretion concomitant with the molecular size distribution of filtered polymer fragments were determined in five normovolaemic men dosed with 30 g/m2 BSA of a species of HES (HES 350/0.60) possessing a M-W of 350 000 combined with an MS of 0.60. Approximately 13% of the total injected dose of HES 350/...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.34.4.361

    authors: Mishler JM,Ricketts CR,Parkhouse EJ

    更新日期:1981-04-01 00:00:00

  • Assessment of suitability of the one step nucleic acid amplification (OSNA) assay as an intraoperative procedure for detection of metastasis in sentinel lymph nodes of breast cancer.

    abstract:AIM:We aimed to assess the one step nucleic acid amplification (OSNA) assay as an intraoperative method in comparison with frozen sections (FS) for detection of metastasis in sentinel lymph nodes (SLNs) of breast cancer. METHOD:100 SLNs from patients with breast carcinoma were enrolled within a 3-month period. Alterna...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2014-202361

    authors: Jara-Lazaro AR,Hussain IH,Thike AA,Wong CY,Ho GH,Yong WS,Ong KW,Madhukumar P,Tan BK,Oey CL,Hwang JS,Tan PH

    更新日期:2014-12-01 00:00:00

  • A comparison of the performance of cystine lactose electrolyte deficient (CLED) agar with Oxoid chromogenic urinary tract infection (CUTI) medium for the isolation and presumptive identification of organisms from urine.

    abstract:AIMS:As part of the UK antimicrobial resistance strategy and action plan, the Public Health Laboratory Service (PHLS) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. Most laboratories report urine Gram negative isolates, as "coliforms" according to morpholo...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.55.7.524

    authors: Fallon D,Andrews N,Frodsham D,Gee B,Howe S,Iliffe A,Nye KJ,Warren RE

    更新日期:2002-07-01 00:00:00

  • An evaluation of the ToxHA test for the detection of antibodies to Toxoplasma gondii in human serum.

    abstract::The titre of antibodies to Toxoplasma gondii was compared in 1985 sera by use of an indirect haemagglutination test (ToxHA test--Wellcome Reagents Ltd) and the dye test. Sera from 42 patients with clinical or serological indications of recent toxoplasmosis were also examined to determine if the ToxHA test would be of ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.33.7.644

    authors: Balfour AH,Bridges JB,Harford JP

    更新日期:1980-07-01 00:00:00

  • Cardiac botryomycosis: an autopsy report.

    abstract::Visceral botryomycosis is rare, and documented sites are lung, brain, kidney, liver and prostate. This report describes a rare autopsy case of disseminated visceral botryomycosis, with bulky, grape-like botryomycotic vegetations in the heart, and similar abscesses in the lungs and bone marrow. This is the first such r...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2008.057588

    authors: Gupta K,Das A,Radotra BD,Bhalla A

    更新日期:2008-08-01 00:00:00

  • Evaluation of the IRIS 939 UDx flow microscope as a screening system for urinary tract infection.

    abstract:AIMS:To evaluate the IRIS flow microscope for the examination of urine specimens and to compare its performance with that of manual methods; in addition, to assess its usefulness as a screening system for the detection of significant bacteriuria in a routine clinical laboratory. METHODS:The IRIS system was compared wi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.56.11.844

    authors: Hughes C,Roebuck MJ

    更新日期:2003-11-01 00:00:00

  • Clinical trial of a chemical test for bacteriuria.

    abstract::A chemical test for the detection of bacteriuria-Uriglox-has been compared with the results obtained on culturing urine obtained by suprapubic aspiration in 351 pregnant or puerperal women. The results of this comparison show a correlation of 96.9%, both false positive and false negative results occurring. ...

    journal_title:Journal of clinical pathology

    pub_type: 临床试验,杂志文章

    doi:10.1136/jcp.26.5.375

    authors: Paterson L,Miller AW

    更新日期:1973-05-01 00:00:00

  • Importance of low serum vitamin B12 and red cell folate concentrations in elderly hospital inpatients.

    abstract::To determine the functional importance of the low B12 and red cell folate concentrations repeatedly observed in the elderly 200 consecutive patients admitted to a geriatric unit were studied. Forty six of the patients had low serum concentrations of B12 (15), red cell folate (26), or both (five). Serum B12 and red cel...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.38.10.1179

    authors: Blundell EL,Matthews JH,Allen SM,Middleton AM,Morris JE,Wickramasinghe SN

    更新日期:1985-10-01 00:00:00

  • Herpesvirus in the oral cavity of children with leukaemia and its impact on the oral bacterial community profile.

    abstract:AIMS:This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia. METHODS:Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2014-202668

    authors: Bezerra TM,Ferreira DC,Carmo FL,Pinheiro R,Leite DC,Cavalcante FS,Belinho RA,Peixoto RS,Rosado AS,dos Santos KR,Castro GF

    更新日期:2015-03-01 00:00:00

  • Radioimmunoassay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: comparative study of children with liver disease.

    abstract::Serum glycocholic acid (SGC) was measured by radioimmunoassay in 277 samples from 122 children with hepatobiliary disorders and from 23 healthy age-matched controls. In patients with hepatobiliary disease the SGC was more frequently abnormal (83%) than values for serum albumin (7%), prothrombin time (17%), bilirubin (...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.9.1011

    authors: Matsui A,Psacharopoulos HT,Mowat AP,Portmann B,Murphy GM

    更新日期:1982-09-01 00:00:00

  • Nuclear beta-catenin and Ki-67 expression in choriocarcinoma and its pre-malignant form.

    abstract:OBJECTIVE:To study the expression of nuclear beta-catenin and Ki-67 in patients with normal gestation products (NGP), complete hydatidiform moles (CHM), and choriocarcinoma to elucidate their roles in carcinogenesis and their interrelations. METHODS:Expression of nuclear beta-catenin and Ki-67 was studied by immunohis...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2005.026666

    authors: Wong SC,Chan AT,Chan JK,Lo YM

    更新日期:2006-04-01 00:00:00

  • Validation of whole slide imaging in the primary diagnosis of gynaecological pathology in a University Hospital.

    abstract:AIMS:Experience in the use of whole slide imaging (WSI) for primary diagnosis in pathology is very limited. We aimed to determine the accuracy of interpretation of WSI compared with conventional light microscopy (CLM) in the diagnosis of routine gynaecological biopsies. METHODS:All gynaecological specimens (n=452) rec...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2014-202524

    authors: Ordi J,Castillo P,Saco A,Del Pino M,Ordi O,Rodríguez-Carunchio L,Ramírez J

    更新日期:2015-01-01 00:00:00

  • Technique for measuring 50% end points in cytotoxicity assays for Clostridium difficile toxins.

    abstract::Serial dilutions of Clostridium difficile culture filtrates were incubated overnight with HeLa cell monolayers. Cells were fixed in formalin, stained with crystal violet, rinsed, and drained. Cell rounding could be observed microscopically in the stained monolayers. Absorbance of the retained dye on monolayers in the ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.39.6.672

    authors: Rothman SW

    更新日期:1986-06-01 00:00:00

  • Expression of the polycomb-group gene BMI1 is related to an unfavourable prognosis in primary nodal DLBCL.

    abstract:BACKGROUND:Clinical outcome in patients with diffuse large B cell lymphomas (DLBCL) is highly variable and poorly predictable. Microarray studies showed that patients with DLBCL with a germinal centre B cell-like (GCB) phenotype have a better prognosis than those with an activated B cell-like (ABC) phenotype. The BMI1 ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2006.038752

    authors: van Galen JC,Muris JJ,Oudejans JJ,Vos W,Giroth CP,Ossenkoppele GJ,Otte AP,Raaphorst FM,Meijer CJ

    更新日期:2007-02-01 00:00:00

  • Microscope slide electrophoresis of serum lipoproteins in agarose gel.

    abstract::An ultramicro electrophoretic technique for the separation of serum lipoproteins is described using agarose gel on microscope slides.The interaction between the agarose gel and lipoproteins has been reduced and the separation between beta- and pre-beta-lipoproteins has been improved over existing methods. A semiquanti...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.24.1.83

    authors: Elphick MC

    更新日期:1971-02-01 00:00:00

  • A family case with germline TSC1 and mtDNA mutations developing bilateral eosinophilic chromophobe renal cell carcinomas without other typical phenotype of tuberous sclerosis.

    abstract:AIM:We examined the genetic alterations in a mother and son with multiple eosinophilic chromophobe renal cell carcinomas (chRCCs) showing no other features. METHODS:Germline DNA and bilateral renal cell carcinoma DNA were genetically analysed by whole-exome sequencing. Candidate gene alterations in the first patient's...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2018-205211

    authors: Sakamoto H,Yamasaki T,Sumiyoshi T,Utsunomiya N,Takeda M,Kamba T,Nakamura E,Ogawa O

    更新日期:2018-10-01 00:00:00

  • A haemagglutination test for anti-Panton-Valentine leucocidin in serum: an interim report on 200 cases.

    abstract::The test is an application of the method originally described by Boyden (1951) and adapted by Stavitsky and Arquilla (1958). Sera which contain anti-Panton-Valentine leucocidin agglutinate the prepared red cells, often to a very high dilution, and this has provided a useful serological test for the diagnosis of staphy...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.14.2.161

    authors: TOWERS AG

    更新日期:1961-03-01 00:00:00

  • Automation in clinical microbiology: a new approach to identifying micro-organisms by automated pattern matching of proteins labelled with 35S-methionine.

    abstract::A new rapid automated method for the identification and classification of microorganisms is described. It is based on the incorporation of 35S-methionine into cellular proteins and subsequent separation of the radiolabelled proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1136/jcp.40.9.1070

    authors: Tabaqchali S,Silman R,Holland D

    更新日期:1987-09-01 00:00:00

  • Nasal polyposis in Peutz-Jeghers syndrome: a distinct histopathological and molecular genetic entity.

    abstract:BACKGROUND:Peutz-Jeghers syndrome (PJS) is an autosomal dominant hamartomatous polyposis syndrome of the gastrointestinal tract, caused by a germline STK11/LKB1 mutation. Nasal polyposis was described in the original report by Peutz. Recently, a molecular-genetic association between nasal polyposis and PJS has been rep...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2005.036418

    authors: de Leng WW,Westerman AM,Weterman MA,Jansen M,van Dekken H,Giardiello FM,de Rooij FW,Paul Wilson JH,Offerhaus GJ,Keller JJ

    更新日期:2007-04-01 00:00:00

  • Histopathological reporting of paediatric cutaneous vascular anomalies in relation to proposed multidisciplinary classification system.

    abstract:BACKGROUND:The terminology applied to vascular anomalies has been variable in previously published literature making interpretation suboptimal. The International Society for the Study of Vascular Anomalies (ISSVA) has proposed a revised classification based on clinical features and histopathological findings. This clas...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2006.038240

    authors: Al-Adnani M,Williams S,Rampling D,Ashworth M,Malone M,Sebire NJ

    更新日期:2006-12-01 00:00:00

  • p53 and hepatoma-derived growth factor expression and their clinicopathological association with Ewing family tumour.

    abstract:PURPOSE:To investigate p53 and hepatoma-derived growth factor (HDGF) expression and their association with clinicopathological features of Ewing family tumour (EFT). EXPERIMENTAL DESIGN:A total of 108 cases of EFT were retrospectively analysed. p53 and HDGF expression were detected using immunohistochemistry, and the ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2013-201705

    authors: Yang Y,Zhen T,Zhang F,Dai S,Kang L,Liang Y,Xue L,Han A

    更新日期:2014-03-01 00:00:00

  • Change of blood group from A2 to Ax in a child with congenital abnormalities.

    abstract::A blood group change from A2 to Ax is described in a boy who had Fallot's tetralogy, oesophageal atresia, and a tracheo-oesophageal fistula. The change of blood group created a transfusion problem. The unusual serology is discussed. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.28.12.962

    authors: Bird GW,Roberts KD,Wingham J

    更新日期:1975-12-01 00:00:00