Abstract:
BACKGROUND:The Sysmex XN haematology instrument performs automatic reflex testing, depending on sample results. A nucleated red blood cell (NRBC) count is provided on all samples. The instrument has a smaller footprint (34%) than previous Sysmex XE analysers. METHODS:An evaluation comparing all results to the Sysmex XE-2100 and manual microscopic differential and morphology (n=390) was performed followed by a workflow study of 1000 samples to compare speed of operation and number of blood films reviews required from both systems. RESULTS:The new features on the instrument are: (1) white cell and NRBC channel, all samples include the NRBC count; (2) white cell precursor channel: false positive flags for blasts, abnormal lymphocytes and atypical lymphocytes are reduced significantly without a statistical increase of false negatives; (3) low white cell count mode: suggested setting of <0.5×10(9)/l. An extended count is more precise and provides an accurate differential. Fluorescent platelet count is performed in a dedicated channel. If the red cell or platelet size histograms are abnormal or if the platelet count is low, then a fluorescent platelet count is automatically performed. Good correlation with the XE-2100 and manual differential was found and the improved results compared to the reference flow cytometric analysis for platelet counts, especially below 30×10(9)/l (XE-2100, R(2)=0.500; XN, R(2)=0.875). CONCLUSION:The XN showed reduced sample turnaround time of 10% and reduced number of blood films for examination, 49% less than the XE-2100 without loss of sensitivity with more precise and accurate results on low cell counts.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Briggs C,Longair I,Kumar P,Singh D,Machin SJdoi
10.1136/jclinpath-2012-200930subject
Has Abstractpub_date
2012-11-01 00:00:00pages
1024-30issue
11eissn
0021-9746issn
1472-4146pii
jclinpath-2012-200930journal_volume
65pub_type
杂志文章abstract::By means of enzyme immunoassays based on two monoclonal antibodies with specificities for distinct forms of placental type alkaline phosphatase (Pl-ALP), together with L-leucine inhibition studies, it has been possible to distinguish the Nagao type carcinoplacental enzyme from other placental type alkaline phosphatase...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.7.826
更新日期:1984-07-01 00:00:00
abstract:AIMS:Strong thyroglobulin immunoreactivity within sinus histiocytes in a lymph node draining a papillary thyroid carcinoma was observed in a recent case. This prompted the investigation of whether thyroglobulin immunoreactivity is common in regional lymph nodes in cases of thyroid malignancy. METHODS:Eighty seven lymp...
journal_title:Journal of clinical pathology
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abstract::Antigenic extracts were prepared from Aspergillus fumigatus, A niger, A flavus and A terreus for use in enzyme linked immunosorbent assay (ELISA) and immunodiffusion (ID) tests for Aspergillus antibodies to determine whether the use of antigenic extracts from species other than A fumigatus increased the sensitivity of...
journal_title:Journal of clinical pathology
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doi:10.1136/jcp.42.11.1215
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abstract::A case of recurrent intussusception in a 76 year old man associated with vascular proliferation is reported. The initial biopsy specimen showed that ulceration and inflammation were not features. The proliferation was so florid as to point to an angiomatous lesion. At this stage a diagnosis of intussusception was not ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.1.91
更新日期:1993-01-01 00:00:00
abstract::The histological appearances of the breast in a patient with mucoviscidosis are those of complete lobular agenesis. Ducts and ductules are well developed, and there is abundant fibrous tissue giving the breast a normal contour. The appearances are contrasted with those of gynaecomastia, and the aetiology is discussed....
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.2.119
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abstract::The excretion of 2,3-dinor-6-keto prostaglandin F1 alpha, a major urinary metabolite of prostacyclin, and the formation of thromboxane B2, a stable metabolite of thromboxane A2, by platelets stimulated by adenosine diphosphate, were studied in alcoholics, who had been admitted for detoxification. Once prolonged heavy ...
journal_title:Journal of clinical pathology
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doi:10.1136/jcp.40.5.512
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abstract::Sixty six women with first or second trimester fetal loss were investigated for the presence of lupus anticoagulant by routine coagulation tests and the dilute Russell's viper venom time with a platelet neutralisation procedure, and for raised anticardiolipin antibodies by an enzyme linked immunosorbent assay. Of 35 w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Chromogenic in situ hybridisation (CISH) is an alternative to immunohistochemistry or FISH for the assessment of HER2 oncogene status in breast cancer. Although CISH is being used increasingly in routine diagnostics, there are no established inter-laboratory quality assurance programmes for this test. METHO...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2007.053850
更新日期:2008-06-01 00:00:00
abstract::The apparent volumes of distribution of Evans blue and (59)Fe-dextran were determined in 18 studies.(59)Fe-dextran volume was 5% lower than Evans blue volume, a highly significant difference. The rate of loss of (59)Fe dextran from the circulation was significantly less than that of Evans blue. ...
journal_title:Journal of clinical pathology
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更新日期:1970-05-01 00:00:00
abstract::During a two-year period of observation Bacteroides species were isolated from specimens of pus and vaginal swabs from 115 patients in this hospital. Thirty-five representative strains proved on examination to be Bacteroides fragilis. Minimal inhibitory and minimal bactericidal concentrations of six antibiotics for th...
journal_title:Journal of clinical pathology
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更新日期:1968-07-01 00:00:00
abstract:BACKGROUND:Currently, no specific immunohistochemical markers are available to differentiate primary mucinous epithelial ovarian cancer (MOC) from adenocarcinomas originating at other sites that have metastasised to the ovary, which may have an impact on patient management and prognosis. AIM:To investigate the express...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2005.034223
更新日期:2007-06-01 00:00:00
abstract::Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.185
更新日期:1967-03-01 00:00:00
abstract::Renal endocrine tumours are extremely rare, and carcinoid tumoral elements in renal cell carcinoma have never been reported. This is the first report of a composite renal cell carcinoma containing a clear cell renal cell carcinoma associated with carcinoid tumoral elements, in a patient with synchronous metastatic dis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.070292
更新日期:2010-02-01 00:00:00
abstract:AIMS:The aim of this study is to explore DNA hypermethylation analysis in sputum and exhaled breath analysis for their complementary, non-invasive diagnostic capacity in lung cancer. METHODS:Sputum samples and exhaled breath were prospectively collected from 20 lung cancer patients and 31 COPD controls (Set 1). An add...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202414
更新日期:2014-08-01 00:00:00
abstract::A study has been made of 523 strains of staphylococci on the basis of biological properties, phage typing, and serology. The value of serology in the identification of pathogenic staphylococci has been assessed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.4.370
更新日期:1970-05-01 00:00:00
abstract::A case is reported of a patient with plasmacytoma which assumed different clinicopathological features over seven years. The tumour first presented as a subcutaneous mass which was misdiagnosed as anaplastic carcinoma. Complete response to radiotherapy ensued but recurrence took the form of an intracerebral tumour. Fu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.10.951
更新日期:1994-10-01 00:00:00
abstract:AIM:To establish whether infection with Epstein-Barr virus (EBV) contributed to the development of coronary artery lesions in a six year old child with an aneurysm and stenoses of the coronary arteries and suspected Kawasaki disease. METHODS:Postmortem paraffin wax sections of the coronary artery and myocardium were e...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.2.161
更新日期:1997-02-01 00:00:00
abstract:AIMS:Traditionally, advanced stage ovarian carcinoma is treated by debulking surgery followed by chemotherapy. However, in some circumstances preoperative chemotherapy may be given before optimal surgical debulking. This study aims to describe the morphological features found in ovarian carcinoma after chemotherapy bec...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.1.27
更新日期:2002-01-01 00:00:00
abstract:AIMS:The aim of this study was to develop a computer-aided diagnosis (CADx) system for identifying breast pathology. METHODS:Two sets of 100 consecutive core needle biopsy (CNB) specimens were collected for test and validation studies. All 200 CNB specimens were stained with antibodies targeting oestrogen receptor (ER...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2017-204478
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:A small subset (10-15%) of gastrointestinal stromal tumours (GISTs) lack mutations in KIT and PDGFRA (wild-type GIST). Recently, a novel BRAF exon 15 mutation (V600E) was detected in imatinib-naive wild-type high-risk intestinal GISTs (4%). However, the frequency and distribution of BRAF mutations within the...
journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.6.499
更新日期:1990-06-01 00:00:00
abstract::Accurate diagnosis of Clostridium difficile infection (CDI) is essential for optimal treatment, prevention and control. There are two reference assays for CDI diagnosis: the cell cytotoxicity assay (CCTA) and toxigenic culture (TC). Importantly, these tests actually detect different targets: CCTA detects the presence ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.080135
更新日期:2011-01-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.4.343
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abstract::Phosphate in both inorganic and organic form is essential for several functions in the body. Plasma phosphate level is maintained by a complex interaction between intestinal absorption, renal tubular reabsorption, and the transcellular movement of phosphate between intracellular fluid and bone storage pools. This home...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2018-205130
更新日期:2019-11-01 00:00:00
abstract::A 45-year-old female patient underwent right hemihepatectomy for metastatic rectal adenocarcinoma. Preoperative imaging demonstrated an area of focal nodular hyperplasia (FNH) in segment VIII and metastatic carcinoma in segment VI of the liver. Gross and microscopic examination of the former lesion showed features typ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.12.967
更新日期:2002-12-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.9.828
更新日期:1992-09-01 00:00:00
abstract::The use of liquid media to detect the production of beta-lactamase by beta-lactamase producing organisms has been compared with the conventional method of inoculation on to agar media. Pharyngeal cultures were obtained from 162 children treated with penicillin for acute tonsillitis. beta-lactamase producing organisms ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.12.1392
更新日期:1984-12-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.031716
更新日期:2006-10-01 00:00:00